Freeze-Drying drying Sales Sheet.pdf · Freeze-drying, or lyophilization, is necessary due to the...
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PO Box 348 Lebanon, NJ 08833 www.opsdiagnostics.com p: 908.253.3444 | f: 908.575.1660 Laboratory Products for Sample Preparation Freeze-drying, or lyophilization, is necessary due to the degradation and instability of certain biomolecules (e.g., proteins and nucleic acids) when stored in aqueous solutions. OPS Diagnostics’ proprietary, ready-to-use, freeze-drying reagents preserve samples during freeze-drying and subsequent storage. Reagents Microbial Freeze Drying Buffer – Filter Sterilized Product No. MFDB 500-06 Microbial Freeze Drying Buffer is a proprietary formulation of plant protein and a carbohydrate lyoprotectant. It has evolved from a proven solution developed by the ATCC, but is free of all animal products/proteins. Viability of bacteria freeze-dried with this reagent far exceeds the performance of freeze-drying methods using sucrose or skim milk over an extended period of time. To use, re-suspend cultured microorganisms in an equal volume of Microbial Freeze Drying Buffer and freeze-dry. Lyophilization Reagent (2X) – Filter Sterilized Product No. LR2X 500-02 This proprietary solution is a combination of a lyoprotectant and matrix-forming excipient that work synergistically to allow for a high primary drying temperature (from -10°C to -5°C) to expedite lyophilization. This unique formulation helps to form a solid matrix and provides excellent stability for proteins, in particular. Reagent can be used at full strength, or diluted 1:1 with samples, prior to freeze-drying. Freeze Drying Indicator – Filter Sterilized Product No. LRFI 500-04 This colorimetric indicator can be used in conjunction with samples undergoing the freeze- drying process in Lyophilization Reagent (2X) to confirm when freeze-drying is complete; it is not intended for use as a lyoprotectant. This reagent is also useful for mapping and validating freeze- dryer units. Key Features Microbial Freeze Drying Buffer ● Ready-to-use ● Contains no animal products or proteins ● More cost effective than BSA formulations ● 24-month shelf life when stored under sterile conditions at room temperature Freeze-Drying Lyophilization Reagent (2X) ● Ready-to-use ● Expedites primary drying phase ● Ideal for proteins Freeze Drying Indicator ● Ready-to-use ● Changing color provides real-time indication of sample dryness Related Products Item Product No. 2 ml Clear Serum Vials * LBVC 02-500-07 5 ml Clear Serum Vials * LBVC 05-500-09 10 ml Clear Serum Vials * LBVC 10-400-11 13 mm Split Rubber Stoppers RSSB 13-500-13 20 mm Split Rubber Stoppers RSSB 20-400-14 13 mm Aluminum Seals TNAS 13-500-15 20 mm Aluminum Seals TNAS 20-400-16 13 mm Crimper KIHC 13-17 20 mm Crimper KIHC 20-18 * also available in amber OPS Diagnostics’ products are intended for research purposes only; not for clinical use
Transcript of Freeze-Drying drying Sales Sheet.pdf · Freeze-drying, or lyophilization, is necessary due to the...
PO Box 348Lebanon, NJ 08833www.opsdiagnostics.comp: 908.253.3444 | f: 908.575.1660Laboratory Products for Sample Preparation
Freeze-drying, or lyophilization, is necessary due to the degradation and instability of certain biomolecules (e.g., proteins and nucleic acids) when stored in aqueous solutions.
OPS Diagnostics’ proprietary, ready-to-use, freeze-drying reagents preserve samples during freeze-drying and subsequent storage.
ReagentsMicrobial Freeze Drying Buffer – Filter SterilizedProduct No. MFDB 500-06
Microbial Freeze Drying Buffer is a proprietary formulation of plant protein and a carbohydrate lyoprotectant. It has evolved from a proven solution developed by the ATCC, but is free of all animal products/proteins. Viability of bacteria freeze-dried with this reagent far exceeds the performance of freeze-drying methods using sucrose or skim milk over an extended period of time. To use, re-suspend cultured microorganisms in an equal volume of Microbial Freeze Drying Buffer and freeze-dry.
Lyophilization Reagent (2X) – Filter Sterilized Product No. LR2X 500-02
This proprietary solution is a combination of a lyoprotectant and matrix-forming excipient that work synergistically to allow for a high primary drying temperature (from -10°C to -5°C) to expedite lyophilization. This unique formulation helps to form a solid matrix and provides excellent stability for proteins, in particular. Reagent can be used at full strength, or diluted 1:1 with samples, prior to freeze-drying.
Freeze Drying Indicator – Filter SterilizedProduct No. LRFI 500-04
This colorimetric indicator can be used in conjunction with samples undergoing the freeze-drying process in Lyophilization Reagent (2X) to confirm when freeze-drying is complete; it is not intended for use as a lyoprotectant. This reagent is also useful for mapping and validating freeze-dryer units.
Key FeaturesMicrobial Freeze Drying Buffer
● Ready-to-use
● Contains no animal products or
proteins
● More cost effective than BSA
formulations
● 24-month shelf life when stored
under sterile conditions at room
temperature
Freeze-Drying
Lyophilization Reagent (2X)
● Ready-to-use
● Expedites primary drying phase
● Ideal for proteins
Freeze Drying Indicator
● Ready-to-use
● Changing color provides real-time
indication of sample dryness
Related ProductsItem Product No.
2 ml Clear Serum Vials * LBVC 02-500-07
5 ml Clear Serum Vials * LBVC 05-500-09
10 ml Clear Serum Vials * LBVC 10-400-11
13 mm Split Rubber Stoppers RSSB 13-500-13
20 mm Split Rubber Stoppers RSSB 20-400-14
13 mm Aluminum Seals TNAS 13-500-15
20 mm Aluminum Seals TNAS 20-400-16
13 mm Crimper KIHC 13-17
20 mm Crimper KIHC 20-18
* also available in amber
OPS Diagnostics’ products are intended for research purposes only; not for clinical use
Viability of E. coli Following Lyophilization Using Three Different Methods The following graph illustrates the ability of Microbial Freeze Drying Buffer, sucrose, and skim milk to protect Escherichia coli following lyophilization. Results are pronounced, demonstrating that Microbial Freeze Drying Buffer greatly improves viability compared to skim milk and sucrose.
Freeze-Drying
Related ProductsExcipients
D-Trehalose — Lyophilization CertifiedProduct No. EXTR 500-05
D-Trehalose is a non-anomeric disaccharide that is extremely useful as a lyoprotectant for freeze-drying. The crystalline dehydrate powder can be added to samples prior to freeze drying in concentrations of 1-2% weight/volume.
Sucrose — Lyophilization CertifiedProduct No. EXSU 500-03
Similar to D-Trehalose, sucrose is a lyoprotectant effective at maintaining the integrity of biological systems during lyophilization. This lyophilization certified disaccharide is a less expensive alternative to D-Trehalose. Sucrose can be added to samples prior to freeze drying in concentrations up to 10% weight/volume.
Mannitol — USP Grade, Lyophilization CertifiedProduct No. EXMN 500-01
Mannitol is a sugar alcohol (polyol) that forms a matrix in solution, effectively preventing sample collapse by allowing water to escape during lyophilization. Mannitol is extremely effective in preserving proteins and biomolecules. This excipient can be added to samples prior to freeze drying in concentrations up to 10% weight/volume.
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Viability of E. coli in Three Lyophilization Solutions
Log
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Months
Skim Milk
Sucrose
MFDB
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PO Box 348Lebanon, NJ 08833www.opsdiagnostics.comp: 908.253.3444 | f: 908.575.1660Laboratory Products for Sample Preparation
