7/24/2019 Formal Report Experiment 2 Extraction

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EXTRACTION

Lorenzo Inigo D. Jimenez, Karen Grace P. Julao, Kyle Dillan T. Lansang, Dixee Allison O. Liao,Bea Patrice G. Liscano, John Wil re! T. "ala#anan

Gro$% & '( Pharmacy Organic (hemistry La#oratory

ABSTRACT

(a eine )as extracte! rom !rie! tea lea*es thro$gh the single extraction techni+$e. A -g tea #ag )as#oile! in a sol$tion o anhy!ro$s so!i$m car#onate an! !istille! )ater, an! then )as %lace! in ase%aratory $nnel )ith -mL o !ichloromethane. The extract )as !raine! into a #ea/er then trans erre!to e*a%orating !ish containing hal s%at$la o anhy!ro$s so!i$m s$l ate. A ter that, it )as co*ere! #y a%er orate! ilter %a%er so that e*erything in the mixt$re exce%t ca eine co$l! e*a%orate as )ell. The%ercent yiel! )as com%$te! #y getting the ratio o the )eight o the ca eine an! )eight o the tea lea*es$se!. The %ercentage yiel! )as &0.

INTRODUCTION

1xtraction is the techni+$e $se! to

se%arate an organic com%o$n! rom a mixt$re o com%o$n!s. The theory o extraction lies in theconce%t o immisci#ility #et)een t)o %hases tose%arate a sol$te rom the other %hase. Thereare t)o main ty%es o extraction in chemistrynamely2 3oli!4Li+$i! extraction an! Li+$i!4Li+$i!1xtraction )hich is $rther !i*i!e! into t)o ty%es5single an! m$lti%le.6 Li+$i!4li+$i! techni+$e is a*ery common metho! in Organic (hemistry.

The ty%e o extraction $se! #y the gro$%

in this ex%eriment )as single li+$i!4li+$i!extraction. In this ex%eriment, the gro$% sho$l!#e a#le to attain the ollo)ing o#7ecti*es2 5 6extract ca eine rom !rie! tea lea*es 5'6/no) the amo$nt o ca eine %resent in -g o tea lea*es, 586 com%are single an! m$lti%leextractions, an! 596 calc$late the %ercentageyiel! or #oth extraction %roce!$res.

EXPERIMENTAL

A. Com oun!" Te"#e! $"am le" u"e!%The #ran! name o the tea sam%le $se! )as "y"ar*el Tahee#o. Ten grams or 9 tea #ags o '.&4gram %ac/s are $se! or the extractionex%eriment.

B. Proce!ure"&. Bo'l'n( o) Tea Lea*e"

A sol$tion o 9.9 g o anhy!ro$s so!i$mcar#onate an! --ml !istille! )ater %lace! in a

'&- mL #ea/er )as heate! *ia )ater #ath $ntil

the soli! !issol*e!. Ten grams o tea lea*escontaine! in tea #ags )ere a!!e! in the mixt$rean! #oile! or - min$tes on lo) lame, the#ea/er #eing co*ere! #y a )atch glass. Thetea#ag )as then remo*e! an! the li+$i! )ass+$eeze! #y %ressing it against the si!es o the#ea/er $sing a glass ro!.

+. S'n(le E #rac#'on

The a+$eo$s sol$tion )as extracte! )ith- "l o !ichloromethane in a se%aratory $nnel.

It )as le t to stan! or ' min$tes 5or more6 $ntilthe se%aration #et)een t)o layers )as clearly*isi#le. The organic layer )as !raine! into aclean #ea/er an! the a+$eo$s layer )as!iscar!e!.

7/24/2019 Formal Report Experiment 2 Extraction

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-'(ure &. S'm le E #rac#'on Se# u

/. O0#a'nmen# o) 1e'(2# o) Ca))e'neCollec#e!

The )eight o the e*a%orating !ish containingthe collecte! ca eine )as o#taine! $sing a

)eighing #alance. The )eight o the resi!$e )aso#taine! #y s$#tracting the )eight o the em%tye*a%orating !ish to the )eight o the e*a%orating!ish containing the collecte! ca eine.

3. Calcula#'on o) Percen#a(e 4'el!

The %ercentage yiel! )as calc$late! #y gettingthe ratio o the )eight o ca eine 5resi!$e6 an!)eight o tea lea*es $se! then m$lti%lying it #y

--0.

RESULTS AND DISCUSSIONS

(a eine )as extracte! rom the !rie! tealea*es thro$gh sim%le extraction metho!.Dichloromethane )as a!!e! to the tea mixt$reonce. The t)o sol$tions )ere mixe! thro$ghmane$*er !one on the se%aratory $nnel.(onse+$ently, t)o layers o !i erent colors )ere%ro!$ce!.

As sho)n in :ig$re , the layers )ere

i!enti ie! as the a+$eo$s layer 5$%%er layer6 an!the organic layer 5lo)er layer6. The a+$eo$slayer )as #ro)n in color, )hich is the tea )hilethe organic layer )as clear, )hich is the!ichloromethane. It sho)s that !ichloromethane%hase is !enser than a+$eo$s sol$tion. Thetannins rom the a+$eo$s sol$tion !i! not mix)ith !ichloromethane !$e to the a!!ition o anhy!ro$s so!i$m car#onate )hich %reci%itatesthe tannins into salts an! ma/e it more sol$#le in)ater. The !ichloromethane %hase ha!

$n!ergone the %rocess o e*a%oration )hereinthe a!!ition o anhy!ro$s so!i$m s$l ate isa%%lie!.

A ter s$#7ecting the tea mixt$re to theentire %rocess o extraction, !ecantation an!e*a%oration, cr$!e ca eine )as collecte! rom -grams o tea lea*es. A %ercentage yiel! o '&0)as com%$te! a ter getting the ratio o the)eight o ca eine 5resi!$e6 an! )eight o tealea*es $se! then m$lti%lying it #y --0. This

means that or e*ery - grams o tea lea*es, anamo$nt o '.& grams can #e collecte! $sing thetechni+$e o sim%le extraction metho!.

DATA OBTAINED

Weight o Tea Lea*es;se! - g

Weight o 1m%ty1*a%orating Dish . "., :arro), :. L.,Pena, G. T., 3arile, A. 3., Torres, P. (. 5'- 96.La#oratory "an$al in Organic (hemistry 5>e*ise!1!ition6. $ezon (ity, Phili%%ines2 ( 1 P$#lishingInc.

'.

http://www.chemicool.com/definition/extraction.html

8. http://www.chem.ubc.ca/courseware/123/tutor ials/exp10

9. Princi%les o 1xtraction an! the 1xtraction o 3emi*olatile Organics rom Li+$i!s

http://www.2.sci.u-szeged.hu/inorg/Sample prepfororganics.pdf

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