Forensic Biology by Richard Li

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Chapter 19: Y-STR Profiling and Gender Typing

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Chapter 19: Y-STR Profiling and Gender Typing. Forensic Biology by Richard Li. Basics. Y chromosome inherited by the father Unique to males Y-STR Sexual assault investigations w/ male suspects Mixtures Number of perpetrators Paternal lineage can be est. Missing person cases. Basics. - PowerPoint PPT Presentation

Transcript of Forensic Biology by Richard Li

Page 1: Forensic Biology by Richard Li

Chapter 19: Y-STR Profiling and Gender Typing

Page 2: Forensic Biology by Richard Li

Y chromosome inherited by the father Unique to males Y-STR

Sexual assault investigations w/ male suspects

Mixtures Number of perpetrators Paternal lineage can be est. Missing person cases

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Disadvantages of Y-STR Discriminating power are low Product rule does not apply Cannot distinguish individuals with the

same paternal lineage

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60 million base pairsTwo regions:

Psuedo-Autosomal Region (PAR) Male Specific Region Y (MSY)

Repetitive elements: STRs Alu LINE SNPs

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400 STR loci have been identified Majority on long arm (60%)

22% on short arm Core repeat sizes:

9% dimeric, 39% trimeric, 45% tetrameric, 9% pentameric, 1% hexameric

Haplotype- collection of alleles that are linked

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European Minimal Haplotype (EMH) 1997 Core set of nine loci DYS19, DYS385a/b, DYS389I, DYS389II, DYS390,

DYS391, DY5392, DY5393

U.S. Haplotype 2003 SWGDAM EMH set plus tow additional loci: DYS438 &

DYS439Forensic Biology by Richard Li 6

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Y-STR in forensic casework started in Europe

Office of Chief Medical Examiner in NYC

Facilitated by commercially available kits Y-PLEX 6

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Victim identification & Sexual assault cases

Amelogenin (AMEL) marker usedAMELX & AMELYAMELY null mutations

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