For Internal Use Only

Dot Plot Page

a. Histograms

b. Scatter Plots

c. Flow Rate Plots

d. Hemoglobin Plots

Data Page

a. General run information – Fields 1-10 and 54

b. CBC5R Information – Fields Calibration Factors – Fields 16-22

c. Diagnostic Values – Fields 23-34 and 51-53

d. Patient Results – Fields 35-49

e. Flags –Field 50For Internal Use Only

FSL HISTOGRAMFSH HISTOGRAMEXT HISTOGRAMRAS HISTOGRAMTOF HISTOGRAM

RED HISTOGRAMS

For Internal Use Only

EX

T

RAS

EXT vs RAS

RED SCATTER PLOTS

FSH

FS

L

FSL vs FSHEXT vs TOF

EX

T

TOFFor Internal Use Only

WHITE HISTOGRAMS

For Internal Use Only

FSL HISTOGRAMFSH HISTOGRAMEXT HISTOGRAMRAS HISTOGRAMTOF HISTOGRAM

WHITE SCATTER PLOTS

For Internal Use Only

EXT vs RAS

EX

TRAS

TOF vs FSH

TO

FFSH

TOF vs RAS int.

TO

FRAS int.

FLOW RATE PLOTS

For Internal Use Only

RED

WHITE

HGB PLOTS

For Internal Use Only

RED

WHITE

For Internal Use Only

1. Compare the two Hemoglobin Values to check dilutions

2. EXT vs RAS for Red Run

3. EXT vs RAS for White Run

4. TOF vs FSH for White Run

THE BASICS OF DOT PLOT ANALYSIS

For Internal Use Only

HGB value from white run

HGB value from red run

Comparing the two HGB values to check dilutions.

1. The two hemoglobin values are obtained from separate dilutions

2. The Red HGB is measured with the blue LED while the HGB is still inside the RBCs

3. The White HGB is measured with the green LED after the HGB has been released from the RBCs – this is the reported value

4. The two values are usually within a few g/dL of each other.

For Internal Use Only

White HGB = 13.51

Red HGB = 12.43

HGB values are close

Conclusion:

Dilutions were appropriate

For Internal Use Only

EXT - RAS for the Red Run

For Internal Use Only

EXT - RAS for the Red Run

RED BLOOD CELLSFor Internal Use Only

EXT - RAS for the Red Run

PLATELETSFor Internal Use Only

EXT - RAS for the Red Run

RBC FRAGMENTSFor Internal Use Only

EXT - RAS for the Red Run

RETICULOCYTESFor Internal Use Only

EXT - RAS for the Red Run

DOUBLETSFor Internal Use Only

For Internal Use Only

TOF-FSH for the White Run

For Internal Use Only

TOF-FSH for the White Run

For Internal Use Only

TOF-FSH for the White Run

Unlysed Red Blood Cells (uRBCs)

For Internal Use Only

EXT-RAS for the White Run

For Internal Use Only

EXT-RAS for the White Run

For Internal Use Only

EXT-RAS for the White Run

Qualibeads

For Internal Use Only

EXT-RAS for the White Run

Lymphocytes

For Internal Use Only

EXT-RAS for the White Run

Monocytes

For Internal Use Only

EXT-RAS for the White Run

Neutrophils

For Internal Use Only

EXT-RAS for the White Run

Eosinophils

For Internal Use Only

EXT-RAS for the White Run

Basophils

For Internal Use Only

EXT-RAS for the White Run

R7 – Residual WBCs from Red

Run

For Internal Use Only

EXT-RAS for the White Run

R8 – Platelets, Aggregated Platelets,

and sometimes Decayed Neutrophils

For Internal Use Only

Differences Between Species

1. Appearance of RBC population on EXT-RAS

2. Location of Eosinophils on scatter plots

3. General size of the Unlysed RBC population

4. Tendency for platelets to aggregate during

the blood draw

Appearance of RBC Population on EXT - RAS

Canine Feline Equine

For Internal Use Only

Location of Eosinophils on Scatter Plots

Canine

Left of Neuts

on EXT-RAS

Feline

Right of Neuts

on EXT - RAS

Equine

Right on Neuts

on TOF - FSH

For Internal Use Only

General Size of Unlysed RBC Population

Canine

Few to Moderate

Numbers uRBC

Feline

Large Numbers

uRBC

Equine

No uRBC to

Few uRBC

For Internal Use Only

Tendency for Platelets to Aggregate During the Blood Draw

Equine – Platelets will Rarely Aggregate

Canine – Platelets will Sometimes Aggregate

Feline – Platelets will Frequently Aggregate

For Internal Use Only

For Internal Use Only

High Platelet Aggregates vs No Platelet Aggregates

Platelet Aggregates

No Platelet Aggregates

Questions?

Practice Analyzing Dotplots