MatricesMultiple food matrices tested, including berries, fruit, minced meat, water and seafood (e.g. Pacific oysters, shrimp, mussels, tuna steaks).

Specificity100% specificity, 10 different virus species have been tested, comprising several virus families with RNA and DNA genomes.

SensitivityLimit of detection (LOD): 3 virus copies per reaction for HAV- and NoV GI RNA and 10 virus copies per reaction for NoV GII RNA.

For safer food – BIOTECON Diagnostics –simply builds up trust!

Food- and waterborne outbreaks involving hepatitis A virus (HAV) and norovirus (NoV)

have been linked to different contaminated matrices, including berries, fruits and

vegetables, seafood and fish, meat, dairy products and drinking water.

The unique, all-in-one, multiplex real-time RT-PCR test, foodproof® Norovirus (GI, GII)

plus Hepatitis A Virus Detection Kit allows simultaneous, qualitative detection and

differentiation of HAV, NoV (genogroups I, II) and a Process Control, bacteriophage

MS2. BIOTECON Diagnostics offers dedicated foodproof® virus detection kits for NoV

and HAV separately as well as together in our multiplex assay. Each kit includes a

solution of the Process Control, which can be added to the sample. The virus kits

allow simple and safe detection of viral RNA and Process Control for each sample in

parallel.

Safe: 3 controls included - positive,

negative and Process Control.

Recovery rates: Calculation possible

with a four-point calibration curve.

ISO/TS 15216 - compliance: The Pro-

cess Control (solution of bacteriophage

MS2) has to be added at the start of the

sample processing.

Easy: Add 10 μl RNA sample in one step

to the mastermix.

RNA extraction kit: Manual or semi-

automated (using a vacuum manifold).

Experienced: Manufacturer of PCR-

based rapid tests for the food industry

since 1998 with an ISO 17025 accredited

service lab.

RT-PCR Multiplex Assay

Norovirus (GI, GII) plus Hepatitis A Virus Detection Kit

foodproof®

Instrument Compatibility

5‘Nuclease / TaqMan® cycler (e.g. PikoReal 24, AriaMx, LC480, LC 96, ABI 7500)

BIOTECON Diagnostics GmbH

Hermannswerder 17 14473 Potsdam Germany

Phone: +49 (0)331-2300-200 Fax: +49 (0)331-2300-299

bcd@bc-diagnostics.com www.bc-diagnostics.com

Workflow

Startvirus concentration*

RNA Extractionapprox. 45 min

PCR Setupapprox. 10 min

Real-Time PCR Run140 min

Ana

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entr

atio

n no

t in

clud

ed)

* addition of bacteriophage MS2 (included in the detection kit)

Storage at -15 °C to -25 °C64 Reactions with a final volume of 25 µl

foodproof® is a registered trademark of BIOTECON Diagnostics.The above mentioned real-time PCR instruments are registered trademarks of their respective holders.

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Real-Time RT-PCR Kits R 302 50 foodproof® Norovirus (GI, GII) plus Hepatitis A Virus Detection Kit

R 302 37 foodproof® Hepatitis A Virus Detection Kit

R 302 38.1 foodproof® Norovirus Detection Kit (GI, GII)

RNA Extraction KitS 400 16 foodproof® Sample Preparation Kit IV