Fluorescent Multiplex IHC Services

Overview

Indivumed’s Fluorescent Multiplex IHC (mIHC) services allow for an analysis of multiple biomarkers on single FFPE tissue sections at highest specificity. Our dedicated scientists follow streamlined workflows, ensuring highest quality standards of mIHC assay development, validation, and analysis while maintaining short turnover times.

Your benefits

• Visualize co-expression of multiple markers in a single section• Reveal spatial relationships of markers and cells• Characterize composition of the tumor immune cell landscape• Save valuable biological material • Benefit from our market-leading tissue quality

Technical details

• Five-color multiplex staining (including DAPI) of FFPE tissue• Highest sensitivity by tyramine signal amplification (TSA)• Automated and highly reproducible staining on the Leica Bond

RX platform, ensuring greatest robustness of our assays• High-resolution whole slide scans with the Zeiss Axio Scan.Z1

Complementary services

• State-of-the-art digital histopathology• Slide reading by experienced in-house pathologists• High-quality biospecimens from the Indivumed biobank

Product Profile No. 03

CD3

CK

CD8

FoxP3

10 µm

10 µm 10 µm

10 µmIH

C PP

2.0

/200

/01.

2018

50 µm

ContactEurope Tel.: +49 (40) 41 33 83 18USA Tel.: +1 (570) 768 4053

Email: CustServ@indivumed.comwww.indivumed.com

Specifications

Indivumed conducts highly standardized, automated immuno-histochemistry (IHC) to support preclinical and clinical R&D. More than one hundred IHC assays, covering important cancer-related and immune markers are established.

Assay development

• RobustIHCassaysensuringhighsensitivityandlowbackground• Validationcomplieswithstandardclinicaltrialsrequirements(specificity,accuracy,linearity,precision)

• Development process utilizes commercial or proprietary antibodies

• Customer-developedassayscanbeutilizedbyIndivumed

Prevalence studies

• Fully validated IHC assays and IVD reagents• Biomarker analysis under GCP compliance• High-resolutionwholeslidescanning

Additionaltechnologies

• RNA in situhybridizationusingRNAscopetechnology• DNA in situhybridizationusingVentanaprobes• Digital pathology

Immunohistochemistry Services

Product Profile No. 03

Workflow of Assay Development

Milestone ISpecificity Testing and Pre-selection of AntibodiesValidation: Specificity

Milestone IIImmunohistochemistry Assay DevelopmentValidation: Specificity and Accuracy

Milestone IIIFull Assay ValidationValidation: Linearity, Sensitivity, Precision

CD3/CD8 Vimentin

About Indivumed

Indivumed GmbH is a physician-led, integrated global oncology company with the world‘s premier high-content tumor database and highest quality biobank. Indivumed’s standard operating procedures are trusted as the global benchmark for biospecimen and clinical data collection. Our long-standing biobanking expertise is ISO 9001-certified and includes strict protocols for tissue sampling and processing with regard to crucial parameters such as tumor region, ischemia time, and information management. The “Indivumed Standard“ means that when choosing our products and services, you are assured of receiving only the highest quality.