FluoBolt –Noggin€¦ · FIA-1701 Ultra-Sensitive, Single-step Noggin Immunoassay Signal Enhanced...
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FluoBolt ™ –Noggin FIA-1701 Ultra-Sensitive, Single-step Noggin Immunoassay Signal Enhanced Fluorescence Immunoassay in a standard 96-well format Assay Characteristics Method Metal Enhanced Direct Sandwich Fluorescence Immunoassay in 96-well plate format Sample type Serum, Plasma Standard range 0-16 ng/mL (6 standards and 2 controls in a human serum based matrix) Conversion factor 1 pg/mL = 0.015 pmol/L (MW: 66 kD) Sample volume 20 µl (undiluted sample) / well Incubation steps / time / temperature Single-step assay, 4 h / 37°C or overnight / RT Sensitivity LOD (0 pmol/L + 3 SD): 1 pmol/L; LLOQ: 0.3 ng/mL Specificity The assay detects circulating uncomplexed human Noggin Cross reactivity Noggin is highly conserved across species. Human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin. Cross reactivity of this assay with other species than human has not been tested. About FluoBolt ™ –Technology DETECT THE UNDETECTABLE FluoBolt ™ Technology is based on a physical effect called “Metal Enhanced Fluorescence” which is generated by metal nano structures on the bottom of our microplates. The unique features of FluoBolt™ Technology enable us to develop direct fluorescence immunoassays with the following benefits: • High Sensitivity • Single-step Procedure • No Washing Steps • No Enzyme Substrate required • Long-term Stable Signals • No special instrumentation required
Transcript of FluoBolt –Noggin€¦ · FIA-1701 Ultra-Sensitive, Single-step Noggin Immunoassay Signal Enhanced...
FluoBolt™–NogginFIA-1701
Ultra-Sensitive, Single-step Noggin ImmunoassaySignal Enhanced Fluorescence Immunoassay in a standard 96-well format
Assay Characteristics
Method Metal Enhanced Direct Sandwich Fluorescence Immunoassay in 96-well plate format
Sample type Serum, Plasma
Standard range 0-16 ng/mL (6 standards and 2 controls in a human serum based matrix)
Conversion factor 1 pg/mL = 0.015 pmol/L (MW: 66 kD)
Sample volume 20 µl (undiluted sample) / well
Incubation steps / time / temperature Single-step assay, 4 h / 37°C or overnight / RT
Sensitivity LOD (0 pmol/L + 3 SD): 1 pmol/L; LLOQ: 0.3 ng/mL
Specificity The assay detects circulating uncomplexed human Noggin
Cross reactivityNoggin is highly conserved across species. Human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin. Cross
reactivity of this assay with other species than human has not been tested.
About FluoBolt™–Technology
DETECT THE UNDETECTABLE
FluoBolt™ Technology is based on a physical effect called “Metal Enhanced Fluorescence” which is generated by metal nano structures on the bottom of our microplates. The unique features of FluoBolt™ Technology enable us to develop direct fluorescence immunoassays with the following benefits:
• High Sensitivity
• Single-step Procedure
• No Washing Steps
• No Enzyme Substrate required
• Long-term Stable Signals
• No special instrumentation required
ML-00-00483Rev02
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*Research use only
About FluoBolt™–NogginFIA-1701
Noggin is a secreted homo dimeric glycoprotein which predominantly binds to BMP-4 and BMP-2 and antagonizes their bioactivities by preventing binding to both Type I and Type II BMP receptors. The FluoBolt™ Noggin assay detects free bioactive, not BMP complexed human Noggin, which was shown by inhibiting the signal of Noggin in human serum with recombinant BMPs.
Noggin is an early developmental biology TGF-beta growth factor that is intrinsic to many tissue development processes and therefore profusely connected to many diseases states. Some of the most commonly investigated are related to bone and nervous system, but Noggin has recently been characterized as an early biomarker in non-alcoholic fatty liver disease (NAFLD).
FluoBolt™ Noggin vs Standard Noggin ELISAs
Supplier Assay Type LOD (pM) LOD (pg/mL) rel Sens. x fold Sens.
Fianostics MEF-FIA 1.5 99 100.0% n.a.
Supplier 1 ELISA 47 3,100 3.2% 31
Supplier 2 ELISA 47 3,100 3.2% 31
Supplier 3 ELISA 47 3,100 3.2% 31
Supplier 4 ELISA 236 15,600 0.6% 157
Supplier 5 ELISA 473 31,200 0.3% 315
Also available:
FluoBolt™ Asporin, FIA-1702
FluoBolt™ Periostin, FIA-1703
FluoBolt™ α-Klotho, FIA-1704
Increased Noggin Detection Sensitivity with MEF Technology vs ELISA Noggin- New NAFLD Biomarker
MEF microplate
Standard microplate
Human Noggin (pM)
Figure: Serum levels of Human Noggin were determined using FluoBolt Noggin. Subjects with simple steatosis (SS) or Nonalcoholic steatohepatitis (NASH) show decreased Noggin levels compared to normal subjects.
