FluidFM micropipette FluidFM nanosyringe FluidFM nanopipette

2, 4, 8 µm

aperture

600 nm

aperture

300 nm

aperture

Adhesion of single cells

Colloidal spectroscopy

Cell isolation

Nano-injection into single cells

Nano-extraction from single cells Spotting

Adhesion of single bacteria

Nano- printing

70+PUBLICATIONS

100% AFMCAPABILITIES

The FluidFM® ADD-ON greatly extends the application scope of classical atomic force microscopes beyond imaging and force spectroscopy.

TAILOREDTO YOUR AFM

Take advantage of more than 8 distinctive applications, now accessible thanks to Fluidic Force Microscopy (FluidFM). This technology is a unique combination of microfluidics and atomic force microscopy. Each type of patented hollow FluidFM probe unlocks a wealth of new applications for AFM users; from single cell adhesion via nano-injection, through to single cells and colloidal studies, to nano-printing, and beyond.

Adhesion of single cellsA HeLa cell is detached from adherent culture with a FluidFM micropipette. The adhesion force is quantified during the process. 10-fold higher throughput and force range can be achieved vs. standard SCFS. Works also for yeast, algae, bacteria and other microbes.

Nano-injection into single cellsFluorescent tracer is injected into HeLa cells with a FluidFM nanosyringe. The injected volumes can be quantified through image analysis and are typically in the range of 50 fL to 300 fL.

Nano-printingNano-printing of 2D structures is possible with a FluidFM nanopipette in air or water and using a wide range of inks and viscosities.

UNLEASH YOUR AFM. With the three distinct FluidFM probe families you get access to unique and high impact applications. More information on the diverse applications: www.cytosurge.com/r/fluidfm-addon

SELECTED APPLICATIONS.

The FluidFM ADD-ON is available for specific AFMs from:

10 µm100 µm 30 µm

FluidFM® ADD-ON.

POWERFUL UPGRADE SOLUTION FOR AFM SYSTEMS.

www.cytosurge.com/r/fluidfm-addon

At the core of all FluidFM systems are the patented hollow probes enabling all FluidFM applications.

FluidFM probes are easy to use and are tailored to the specific geometrical and physical requirements of any compatible AFM system. A FluidFM probe consists of two parts:• The pre-glued FluidFM chip which integrates the microfluidics as well as the hollow cantilever.• The polymer adapter which is tailored to the specific AFM. It integrates a liquid reservoir

and ensures a tight and reliable pneumatic connection up to the cantilever, as well as precise positioning for the AFM feedback.

Cantilever. Pyramidal tip of the nanosyringe with a 600 nm aperture.

Intranuclear injection with the nanosyringe.

ZOOM IN: FluidFM NANOSYRINGE.

FluidFM® PROBE.

CORE TECHNOLOGY

FluidFM cantilever

Liquid reservoir

1.5 mmFluidFM chip

FluidFM probe. All FluidFM chips come pre-glued to an AFM-specific adapter.

FluidFM adapter

AFM-SPECIFIC FluidFM PROBES.FluidFM probe with a specific adapter for JPK and Nanosurf AFMs.

FluidFM probe with a specific adapter for Bruker AFMs.

30 µm 1 µm 30 µm

Subject to change without notice. Revision: 1.0 Copyright © 2020 Cytosurge AG, Switzerland

CYTOSURGE AG, SÄGEREISTRASSE 25, 8152 GLATTBRUGG, CHPHONE +41 43 544 87 00, WWW.CYTOSURGE.COM