Flowcytometry Workshop - Tanta Universityorl.tanta.edu.eg/cecr/Research Team/ICBS Workshop.pdf ·...
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ﺑﺎﻹﺷﺘﺮﺍﻙ ﻣﻊUnder the Patronage of Prof. Ashraf El-Shihy Minister of Higher Education Prof. Ibrahim Salem Vice President of Tanta University Acting President of Tanta University Honory Chairman Prof. Tarek M. Fayed, Dean of Faculty of Science Chairmen Prof. Mohamed H. Mona, President of ICBS Prof. Khadiga Gaafar, President of EGSZ Chair, Scientific and Organizing Committee Prof. Mohamed L. Salem, Prof. of Immunology, Faculty of Science Tanta University, 9-10 November, 2016 ﺍﳌﺆﲤﺮ ﺍﻟﺪﻭﱃ ﺍﻟﺜﺎﻣﻦ ﻟﻠﻌﻠﻮﻡ ﺍﻟﺒﻴﻮﻟﻮﺟﻴﺔ ﻟﻠﺠﻤﻌﻴﺔ ﺍﳌﺼﺮﻳﺔ ﺍﻷﳌﺎﻧﻴﺔ ﻟﻌﻠﻢ ﺍﳊﻴﻮﺍﻥ21 ﺍﳌﺆﲤﺮ ﺍﻟﺪﻭﱃTHE 8 th International Conference on Biological Sciences in Collaboration with 21 st International Conference of Egyptian German Society of Zoology TANTA UNIVESITY FACULTY OF SCIENCE Faculty of Science TANTA UNIVERSITY EGYPT T H E I N T E R N A T I O N A L C O N F E R E N C E O N B I O L O G I C A L S C I E N C E S E G Y P T I A N G E R M A N S O C I E T Y O F Z O O L O G Y ﻥ ﺍ ﻮ ﻴ ﳊ ﺍ ﻢﻠ ﻌ ﻟ ﺔﻴﻧ ﺎ ﳌﻷ ﺍ ﺔﻳ ﺮ ﺼ ﳌ ﺍ ﺔ ﻴ ﻌ ﻤ ﳉ ﺍ١٩٩٠ ١٩٩٠ Under Patronage Prof. Ibrahim Salem President, Tanta University Prof. Amgad Abdel-Raoof Dean, Faculty of Medicine Prof. Tarek Fayed Dean, Faculty of Science Prof. Gamal Mousa Director, Tanta University Educational Hospital Lecturers Prof. Mohamed Labib Salem, PhD Prof. of Immunology, Tanta University Executive Director, CECR Prof. Mohamed Attia Saad, PhD Assistant Prof. of Clinical Pathology, Tanta University Director, Flow Cytometry Unit, CECR Dr. Abdel Aziz Awad Zidan, PhD Lecturer of Immunology, Damanhur University Director, Genomic & Proteomic Unit, CECR Moderators Prof. Said Hammad Abdou, MD Prof. of Clinical Pathology, Tanta University Lab Director, CECR Prof. Mohamed Ramadan Elshanshory, MD Prof. of Hematology & Oncology, Tanta University Clinical Director, CECR Workshop Topics • Basics of Flow Cytometry technology and how does it work • Fluorescene and fluorochromes • Flow Cytometric applications in cell proliferation apoptosis, DNA content and cell cycle • Advantages, disadvantages, limitations and opportunities • Intensive hands-on Training Registration Registration Fees: 500 L.E Including Printed Materials, Lunch Break and Certificates CECR is a project funded by Science & Technology Development Fund (STDF) Flowcytometry Workshop
Transcript of Flowcytometry Workshop - Tanta Universityorl.tanta.edu.eg/cecr/Research Team/ICBS Workshop.pdf ·...
باإلشتراك مع
Under the Patronage of
Prof. Ashraf El-ShihyMinister of Higher Education
Prof. Ibrahim SalemVice President of Tanta University
Acting President of Tanta University
Honory Chairman Prof. Tarek M. Fayed, Dean of Faculty of Science
Chairmen Prof. Mohamed H. Mona, President of ICBSProf. Khadiga Gaafar, President of EGSZ
Chair, Scienti�c and Organizing CommitteeProf. Mohamed L. Salem, Prof. of Immunology, Faculty of Science
Tanta University,9-10 November,
2016
املؤمتر الدوىل الثامن للعلوم البيولوجيةاملؤمتر الدوىل 21 للجمعية املصرية األملانية لعلم احليوان
THE 8th International Conference on Biological Sciences
in Collaboration with 21st International Conference of Egyptian German Society of Zoology
T A N T A U N I V E S I T YF A C U L T Y O F S C I E N C E
Faculty of ScienceTANTA UNIVERSITY
E G Y P T
THE INTERNATION
AL CO
NFERENCE ON BIO
LOG
ICA
L SC
IENCES
EGYP
TIAN
GERMAN SOCIETY OF ZOO
LOG
Y
ناجلمعية املصرية األملانية لعلم احليوا
١٩٩٠١٩٩٠
Under PatronageProf. Ibrahim SalemPresident, Tanta University
Prof. Amgad Abdel-RaoofDean, Faculty of Medicine
Prof. Tarek FayedDean, Faculty of Science
Prof. Gamal MousaDirector, Tanta University Educational Hospital
LecturersProf. Mohamed Labib Salem, PhDProf. of Immunology, Tanta UniversityExecutive Director, CECR
Prof. Mohamed Attia Saad, PhDAssistant Prof. of Clinical Pathology, Tanta UniversityDirector, Flow Cytometry Unit, CECR
Dr. Abdel Aziz Awad Zidan, PhDLecturer of Immunology, Damanhur UniversityDirector, Genomic & Proteomic Unit, CECR
ModeratorsProf. Said Hammad Abdou, MDProf. of Clinical Pathology, Tanta UniversityLab Director, CECR
Prof. Mohamed Ramadan Elshanshory, MDProf. of Hematology & Oncology, Tanta UniversityClinical Director, CECR
Workshop Topics• Basics of Flow Cytometry technology and how does it work• Fluorescene and �uorochromes• Flow Cytometric applications in cell proliferation apoptosis,
DNA content and cell cycle• Advantages, disadvantages, limitations and opportunities• Intensive hands-on Training
RegistrationRegistration Fees: 500 L.EIncluding Printed Materials, Lunch Break and Certi�catesCECR is a project funded by Science & Technology Development Fund (STDF) Flowcytometry
Workshop
Opening and Registration
T H E O R E T I C A L S E S S I O N
Introduction: Basics of �ow cytometry and how does the machine workProf. Mohamed Labib Salem, Director, CECR
Machine and its instrumentations (Fluidic-Optic-Electronic)Prof. Mohamed Attia Saad, Flow Cytometry Unit Director, CECR
C o f f e e B r e a k
Principles of hand on processing of samplesMs. Mona Zidan, MSc,Research and teaching Assistant, CECR
P R A C T I C A L S E S S I O N
Surface Staining: Processing of peripheral blood sampleMs. Mona F. Zidan (GP A) Ms. Sohaila Galal (GP B)Mr. Ismail Attia (GP C)Research Assistants Sta�, CECR
L u n c h B r e a k
Samples AcquisitionMs. Mona F. Zidan, Research and Teaching Assistant, Flow Cytometry Unit, CECR
09:00 - 09:30 AM
09:45 - 10:30 AM
09:45- 10:30 AM
10:45 - 11:15 AM
11:15 - 11:45 AM
12:15 - 02:00 PM
12:15 - 02:00 PM
02:00 - 02:45 PM
02:45 - 03:45 AM
11:45 - 12:15 AM
W O R K S H O P P R O G R A MD A Y 1 : 9 t h N O V E M B E R , 2 0 1 6
Opening and Registration
T H E O R E T I C A L S E S S I O NIntroduction of applications of Flow cytometry applications in research and DiagnosisProf. Mohamed Attia Saad, Flow Cytometry Unit Director, CECR
Flow Cytometric quantitation of DNA content and cell cycleDr. Abdel-Aziz Awad Zidan, Director of Genomics & Proteomics Unit, CECR
C o f f e e B r e a k
Preparation of PBMCsMr. Sohaila Galal, Assistant Lecturer, Tanta University
P R A C T I C A L S E S S I O NIsolation of PBMCs by FicollMs. Mona F. Zidan (GP A) Ms. Sohaila Galal (GP B) Mr. Ismail Attia (GP C)Research Assistants Sta�, CECR
Samples Staining by (Annexin V) for detection of apoptosisMs. Sohaila Galal (GP A) Ms. Mona F. Zidan (GP B) Mr. Ismail Attia (GP C)Research Assistants Sta�, CECR
L u n c h B r e a k
Sample AcquisitionMs. Mona F. Zidan, Research and Teaching Assistant, Flow Cytometry Unit, CECR
Summary, Evaluation and Certi�cation
09:00 - 09:30 AM
10:00 - 10:45 AM
10:00 - 10:45 AM
11:00 - 11:45AM
11:45 - 12:00 AM
12:00 - 12:15 PM
12:30 - 01:15 PM
12:30 - 01:15 PM
01:15 - 01:45 PM
01:45 - 02:30 PM
02:30 - 03:30 PM
03:30 - 04:30 PM
W O R K S H O P P R O G R A MD A Y 2 : 1 0 t h N O V E M B E R , 2 0 1 6
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