First Cloning Experiment Cohen et al., 1973 “Recombinant DNA”

First Cloning Experiment

Cohen et al., 1973

“Recombinant DNA”

Cloning Vectors: pBR322 plasmid

Cloning into pBR322

RT-PCR and Cloning

Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615

“Supermice”

Gene Therapy: Adenovirus vector

Golden Rice

Weeding Out Land MinesFirst Glowing Pet

Polypeptide Polypeptide “X”“X”

Anti-”X” Anti-”X” AntibodiesAntibodies

•Antibodies can be Antibodies can be utilized to detect utilized to detect the presence of the presence of protein “X” in a protein “X” in a

given samplegiven sample

Antibody ProductionAntibody Production

Y YY

Y

Y Y

Western Blot AnalysisWestern Blot Analysis

SDS-PAGESDS-PAGE

Isolate ProteinsIsolate Proteins

P3P3

S6S6

Incubate Incubate with with

AntiseraAntisera

Transfer to nitrocellulos

e

YYYY

Proteomics Approach in Arabidopsis

First Cloning Experiment Cohen et al., 1973 “Recombinant DNA”

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