FIRST AUTHOR: DOINA GHERESCU OTHER AUTHORS: ADRIANA VÂNTU PAUL-CIPRIAN FIC Ă MARCEL PERIAN R Ă...
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Transcript of FIRST AUTHOR: DOINA GHERESCU OTHER AUTHORS: ADRIANA VÂNTU PAUL-CIPRIAN FIC Ă MARCEL PERIAN R Ă...
![Page 1: FIRST AUTHOR: DOINA GHERESCU OTHER AUTHORS: ADRIANA VÂNTU PAUL-CIPRIAN FIC Ă MARCEL PERIAN R Ă ZVAN CONSTANTIN ERBAN Coordinators: Prof. Dr. Dan Dobreanu.](https://reader036.fdocuments.us/reader036/viewer/2022081503/56649efa5503460f94c0cbb7/html5/thumbnails/1.jpg)
Long-term follow-up of type 1 diabetic Wistar rats
FIRST AUTHOR: DOINA GHERȚESCU
OTHER AUTHORS: ADRIANA VÂNTU
PAUL-CIPRIAN FIȘCĂ
MARCEL PERIAN
RĂZVAN CONSTANTIN ȘERBAN
Coordinators: Prof. Dr. Dan Dobreanu Ass. Prof. Dr. Alina Scridon
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Background
Diabetes mellitus (DM) = a complex chronic metabolic disease characterized by:
hyperglycemia
- carbohydrate
impaired - lipid metabolism
- protein
Our study!
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Background
Experimental models ─ essential for clarifying the pathogenesis and progression of the disease.
therapeutic strategies
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Objective
Long-term follow-up on Wistar rats with Streptozotocin induced type 1 Diabetes mellitus
Success and mortality rates
Reinjection – is it efficient?
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Materials and Methods
31 6-week-old Wistar rats
Controln=14
Diabetes mellitus
n=17
Initial bodyweig
ht:177.88 ±
4.01 g 11 weeks – Streptozotocin injection (i.p. 60 mg/kg of bw)
12 weeks – if plasma glucose ≥ 250 mg/dl => Diabetics
< 250 mg/dl => Reinjection
o Streptozotocin (STZ) = a cytotoxic glucose analogue that
preferentially accumulates in pancreatic β-cells.
o The most widely used diabetogenic chemical in DM
research
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Materials and Methods
For 28 weeks
weekly
Bodyweight
Food intake
Water intake
11 and 38 weeks
SystolicBP
HeartRate
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Materials and MethodsNoninvasive photopletismography method using a pneumatic tail cuff
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ResultsSucces rates of Streptozotocin administration
58.82%29.41%
11.77%
Succes Rates
1 injection 2 injections Failure
Overall success: 88.23%
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ResultsMortality rates
Control => 0 deathsDiabetes mellitus => 4 deaths out of 17 rats (23.5 %)
2 STZ-reinjected
(unsuccessfull)
2 DM-related
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ResultsLong-term evolution of bodyweight
5 10 15 20 25 30 35 40100
200
300
400
500
600
Bodyweight
Control DM
Age (weeks)
Bodyw
eig
ht
(g)
Spearman r = 0.71, p< 0.001
Spearman r = 0.30, p< 0.001365.46 ± 10.03g
511.43 ± 13.54g
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ResultsLong-term evolution of food intake
6 11 16 21 26 31 36 410
10
20
30
40
50
60 Food intakeControl DM
Age (weeks)
Fo
od
in
take
(g
/24
h)
44.64 ± 2.31 g/24h
25.86 ± 0.88 g/24h
p<0.001
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ResultsLong-term evolution of water intake
6 11 16 21 26 31 36 410
50
100
150
200
250Water intake
Control DM
Age (weeks)
Wate
r in
take (
ml/24 h
)
78.57 ± 2.43 ml/24h
210.71 ± 13.55 ml/24h
p<0.001
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ResultsSystolic blood pressure
11 weeks 38 weeks0
40
80
120
160
126.07141.71
127.41 134.50
Systolic Blood Pressure
Age
Sys
tolic
Blo
od P
ress
ure
(m
mH
g)
p<0.01
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ResultsHeart rate
11 weeks 38 weeks0
100
200
300
400
319.21388.57
311.53 293.10
Heart Rate
Age
Heart
Rate
(bpm
)
p<0.001
p<0.001
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Results1 STZ vs 2 STZ
0
100
200
300
400
500
600
377.63
47.25
215.63
137.2
286.43
521.63
346
39.2
190
130.2
279.4
489.21 STZ vs 2 STZ
Diabetes mellitus (1 STZ) Diabetes mellitus (2 STZ)
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Discussion
High success Low mortality
rates
Diabetic rats - a modest gain in bodyweight + significantly increased food and water consumption
STZ-induced DM model in Wistar rats mimics the clinical signs of human patients with type
1 DM
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Discussion
The different response in SBP and HR - impaired autonomic balance with vagal hyperactivity
STZ reinjection - safe and efficient => a fact of great procedural importance in long-term studies
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Conclussion
One of the most detailed, long-term follow-ups of the most widely used experimental model of type 1 DM
▪The first one to demonstrate that STZ can be safely reinjected, without any changes in rats’ outcomes
▪The STZ-diabetic rat – useful experimental model that reliably replicates many of the major clinical signs of type 1 human DM
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Thank you very much for your attention!