Finger Print of Life. What does DNA look like? macroscopic view: nanoscopic view:
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Transcript of Finger Print of Life. What does DNA look like? macroscopic view: nanoscopic view:
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Finger Print of LifeFinger Print of Life
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What does DNA look like?
macroscopic view: nanoscopic view:
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DNA is an organism’s “blueprint”
Tells your cells what proteins they need to make Proteins determine what physical traits and characteristics
you (and all organisms) have
General Function of DNA
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There are three parts to every nucleotide:
There are four nitrogenous bases: Adenine, Thymine, Cytosine, Guanine
Nucleotides
a phosphate group a 5-carbon sugar“deoxyribose”
1 of 4 nitrogenous(nitrogen-containing)
bases
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DNA (DeoxyriboNucleic Acid) is a double helix
Backbone is made of sugar-phosphate
Base pairs bind the backbone together
Adenine always pairs with Thymine
Guanine binds with Cytosine
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Published in 1871 First to isolate and identify DNA and suggested its role in
heredity.
Friedrich Miescher
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Published in 1950 Found that the amount of adenine was the same as
thymine and the amount of cytosine was the same as guanine in any DNA sample
Erwin Chargaff
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Worked together ~1950 Studied DNA using x-ray diffraction techniques
Both worked together (on and off again) at King’s College in London
Maurice Wilkins & Rosalind Franklin
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Published in 1953 Determined the structure of DNA (a double helix) and
published a one-page article in Nature in April 1953
James Watson & Francis Crick
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The Double Helix Watson & Crick explained
that hydrogen bonds hold the nitrogen bases together: A always pairs with T C always pairs with G
Hydrogen bonds provide just enough force to hold the strands together, yet can be easily broken if needed.
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Before a cell divides, it must make a copy of its genetic
material. DNA is copied in a process called
DNA Replication “Each strand has all the information needed to reconstruct
the other half by the process of base-pairing!”-Watson & Crick
How does DNA make copies?
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1. DNA helicase (an enzyme) “unzips” the
base pairs, unwinding the DNA double helix
2. Free DNA nucleotides find their complementary bases along the new strands
3. DNA polymerase (an enzyme) helps assemble the sugar-phosphate backbone to complete the new DNA strands
DNA Replication
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The result is two DNA molecules identical to each other
and to the original molecule Each DNA molecule has one original strand and one new
strand; we call this the semi-conservative replication process.
DNA Replication
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Watch the following video clip to see an animation of how
DNA replication works!
http://www.youtube.com/watch?v=2iVltkYy0jg&safe=active
DNA Replication
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Base pairs A-T – double hydrogen
bond G -C – triple hydrogen
bond
Nucleotide (3 chemical groups) Sugar – deoxyribose
contains 5 Carbon atoms
Phosphate group A base (A, T, G, C)
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How Does DNA Copy Itself?How Does DNA Copy Itself?
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History of Replication
Watson & Crick – realized that complementary base pairing provided a way for DNA to copy itself - base pairing could allow a new strand to be built on an old strand - 3 possible models of DNA replication
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3 Models of Replication
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3 Models of Replication:
Semi-conservative Replication Each strand in a DNA molecule is used as a template to build a new strand using complementary base pairing Results in new molecule with one original DNA strand and one new strand
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3 Models of Replication:
Conservative Replication
Leaves the original DNA intact and produces another molecule of DNA identical to the first
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3 Models of Replication:Dispersive Replication
Creates two molecules that are a mix of new and old DNA interspersed along each strand of the molecule
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The Question is Answered
1957 Matthew Meselson & Franklin Stahl -conducted an experiment proving replication is semiconservative - each DNA molecule has one new strand and one old strand
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DNA Replication: Process
Replication begins when the enzyme DNA helicase opens the DNA forming replication bubbles
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DNA Replication: Process
Multiple replication bubbles are opened simultaneously allows the molecule to be replicated quickly
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DNA Replication: Process
The nitrogen bases on the original DNA strands are exposed in the replication bubbles. They serve as a template to build new DNA strands
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DNA Replication
The ends of the replication bubbles known as the
replication fork is where replication begins
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DNA Replication – DNA Polymerase
The enzyme DNA polymerase brings new nucleotides to the replication fork - it pairs them according to base
pairing rules A pairs with T C pairs with G
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DNA Replication – Leading Strand
The process of replication proceeds from 5’ to 3’ leading strand- replication is continuous – it is built toward the
replication fork
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DNA Replication – Lagging Strand
lagging strand- replication occurs in short segments - called Okazaki fragments - the new DNA strand grows away from the replication fork
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DNA Replication- The Big Picture
Each Bubble has 2 Forks – each fork has a leading and lagging strand
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DNA Replication
The process continues until 2 complete copies of the DNA are produced
Each copy of the DNA contains one strand of
DNA from the original DNA molecule and one new strand that was produced by replication
Known as semi-conservative replication
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DNA Replication
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DNA Replication-
Vocabulary
replication DNA helicase replication bubble replication fork DNA polymerase leading strand lagging strand Okazaki Fragment semi-conservative replication
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DNA replication
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Multiple Replication Forks During Multiple Replication Forks During EukaryoticEukaryotic DNA Synthesis DNA Synthesis
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MUTATIONSMUTATIONS Changes in DNA that affect genetic
information
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Gene MutationsGene Mutations Point Mutations – changes in one
or a few nucleotides– Substitution
THE FAT CAT ATE THE RAT THE FAT HAT ATE THE RAT
– Insertion THE FAT CAT ATE THE RAT THE FAT CAT XLW ATE THE
RAT– Deletion
THE FAT CAT ATE THE RAT THE FAT ATE THE RAT
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Chromosome MutationsChromosome MutationsChanges in number and structure of
entire chromosomes Original Chromosome ABC * DEFDeletion AC * DEFDuplication ABBC * DEFTransposition ACB * DEFInversion AED * CBFTranslocation ABC * JKL
GHI * DEF
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Significance of MutationsSignificance of Mutations• Most are neutral
• Eye color• Birth marks
• Some are harmful• Cystic Fibrosis• Down Syndrome
• Some are beneficial• Sickle Cell Anemia to Malaria• Immunity to HIV
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What Causes Mutations?What Causes Mutations? There are two ways in which DNA can
become mutated:– Mutations can be inherited.
Parent to child– Mutations can be acquired.
Environmental damageMistakes when DNA is copied