Final stuff:Final stuff: Lab practicalLab practical

– Coleoptera, HemipteraColeoptera, Hemiptera

Final exam: Fri May 2 @ 2:15Final exam: Fri May 2 @ 2:15– Assessment with InvertebratesAssessment with Invertebrates

Lecture material (IDEM protocol)Lecture material (IDEM protocol) Stone et al. (2005): Ag streams in Stone et al. (2005): Ag streams in

IllinoisIllinois Signatures of ICI; Ohio EPA use of ICI Signatures of ICI; Ohio EPA use of ICI

TodayToday

IDEM samplingIDEM sampling

Stone et al. (2005) paperStone et al. (2005) paper

Assessment exerciseAssessment exercise

MACROINVERTEBRATE COMMUNITY

MONITORING EXAMPLE

INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT

STAGES OF BIOLOGICAL COMMUNITY ASSESSMENT

DATA REDUCTION AND ANALYSIS

FIELD PROCESSING LABORATORY PROCESSING

SAMPLE COLLECTION

EcologicalIntegrity

What are We Trying to Measure ??

Water Quality

Habitat, Flow regime

Biological Interactions

“The ability of an aquatic ecosystem to support and maintain a balanced, integrated, adaptive community of

organisms having a species composition, diversity, and functional organization

comparable to the best natural habitats within a region.”

BIOLOGICAL INTEGRITY

STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY

ASSESSMENTASSESSMENT

DATA REDUCTION AND ANALYSIS

FIELD PROCESSING

LABORATORY PROCESSING

SAMPLE COLLECTION

Aquatic Larvae

Aquatic Adults

Terrestrial Adults

Adult and Larval Caddisflies

(Trichoptera)

USEPA Rapid Bioassessment Protocols

Riffle Habitats

Two Composite

KICK Screen Samples

STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY

ASSESSMENTASSESSMENT

DATA REDUCTION AND ANALYSIS

FIELD PROCESSING

LABORATORY PROCESSING

SAMPLE COLLECTION

Sample Elutriation

Remove Gravel and Sand

Remove Excess CPOM

The Final Sample Ready for

Preservation

Field Preservation of Samples

GPS for Spatial Reference

PAPERWORK…PAPERWORK…. TRIPLE REDUNDANCY

Water Chemistry

Habitat Assessments

Field Observations

STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY

ASSESSMENTASSESSMENT

DATA REDUCTION AND ANALYSIS

FIELD PROCESSING

LABORATORY PROCESSING

SAMPLE COLLECTION

All Samples are Returned to Laboratory for Processing

Squares (25 cmSquares (25 cm22))

Removed by Suction to Removed by Suction to Petri DishPetri Dish

100 Organism Sub-Sample

15 Minute Sort Sub-Sample

100 Organism Sub-Sample

Selected at Random

Second Sub-Sample 15 Minutes

Entire Pan “Randomly” Sorted

Using Forceps

Final Sort Under Microscope

Sub-Samples Processed to Sub-Samples Processed to Family LevelFamily Level

All Samples Preserved for Permanent Museum Storage

STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY

ASSESSMENTASSESSMENT

DATA REDUCTION AND ANALYSIS

FIELD PROCESSING

LABORATORY PROCESSING

SAMPLE COLLECTION

TEN SUB-METRICS

SITE INFORMATIO

NTAXONOMIC

INFORMATION

AND

TAXA COUNTS

LABORATORY BENCHSHEET

Example of 2 Sub-Metrics

E

P

T

EPT Index = 6 Families

EPT Count = 7 Individuals

Metric Scores = 0, 2, 4, 6, 8

SCORING CRITERIA FOR THE FAMILY LEVELMACROINVERTEBRATE INDEX OF BIOTIC INTEGRITY

(mIBI)USING PENTASECTION AND CENTRAL TENDENCY ON THE

LOGARITHMIC TRANSFORMED DATA DISTRIBUTIONS OF THE 1990-1994 RIFFLE KICK SAMPLES

CLASSIFICATION SCORE

8 6 4 2 0

Family LevelHBI

4.08 4.09 - 4.54 4.55- 5.05 5.06 - 5.62 5.63

Number ofTaxa

18 17-15 14-11 10-8 7

Number ofIndividuals

350 349-213 212-130 129-80 79

PercentDominant

Taxa 22.1 22.2-31.1 31.2-43.8 43.9-61.5 61.6

EPTIndex

8 7-6 5-4 3 2

EPTCount

195 194-92 91-43 42-20 19

EPTCount To

TotalNumber ofIndividuals

0.69 0.68-0.47 0.46-0.30 0.29-0.14 0.13

EPT CountTo

ChironomidCount

11.66 11.65-5.71 5.70-2.56 2.55-0.89 0.88

ChironomidCount

6 7-19 20-54 55-146 147

TotalNumber ofIndividualsTo Numberof Squares

Sorted

410 409-172 171-72 71-30 29

Scoring Criteria

for Riffle KICK

Samples

Not All Rivers and Streams Have Riffle Habitats

Multi-Plate Samples

are Collected

IndividuallyMulti-Plate

Samples are Preserved in the Field and

Scraped in the Laboratory

Systematic Community Assessments of Benthic

Macroinvertebrate Communities are Effective

SUMMARY

IBI -- Family Level Multi-Metric Indices Provide:

Cost Effective Data of Ecological Health of Rivers and Streams

Assessment Information to Make

Management Decisions