Final Capstone How bacterial growth is affected using oil and (1)
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Transcript of Final Capstone How bacterial growth is affected using oil and (1)
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How bacterial growth is affected using oil and powder extracts of Curcuma longa?Arry PatelVicki Stanavitch, Dr. Dorothy Anthony, Jason KovalKeystone CollegeDate: 17th April , 2013
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Background Information Curcuma longa (C. longa)
A Perennial herb1
Curcumin, an active ingredient1
Commonly known as “Turmeric” Raw, dried, powder and oil1
Antioxidant, anti-inflammatory, anti-platelet, cholesterol lowering, antibacterial and anti-fungal effects1
Figure 1: Turmeric plant from Turmeric.co.in
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Why I chose this topic? Personal use or learning of
turmeric powder Wondering how Turmeric oil can
be useful? Determine the healing effect
Oil and powder extracts
Figure 2: Turmeric Powder from Mothernaturenetwork
Figure 3: Turmeric Oil by Anand Oil website
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Purpose The purpose of my research is to determine how
bacterial growth is affected using oil and powder extract of Curcuma longa.
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Methods Oil and powder extraction
Soxhlet apparatus Reverse Dean-Stark method
Figure 5: Reverse Dean-Stark from Fischer ScientificFigure 4: Soxhelt Apparatus from
Fischer Scientific
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Methods 7 bacterium
S.aureus, S.albus, S.epidermis, B.cereus, B.subtilis, E.coli, P.aeruginosa
Agar Diffusion Method using Muller Hinton agar medium
3 trials Concentrations:
0.1ml - 0.5ml per trial per bacteria. Incubated the agar plates at 37° C for
24 hours. Measured zone of inhibition in
millimeters.
Bacteria Gram Positive or Gram negative
B.cereus +
B.subtilis +
E.coli -
S.aureus +
S.epidermis +
S.albus +
P.aeruginosa -
Table 1: Bacteria used for the project
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Results
Figure 6: 0.1 ml Turmeric Oil
B.cereus B.subtilis E.coli
S.aureus S.epidermis S.albus
P.aeruginosaGraph 1: Zone of Inhibition at 0.1ml Turmeric oil
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Results
Graph 2 : Zone of Inhibition at 0.2 ml Turmeric oil Figure 7: 0.2 ml Turmeric oil
B.cereus B.subtilis E.coli
S.aureus S.epidermis S.albus
P.aeruginosa
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Results
Figure 8: 0.3 ml Turmeric Oil P.aeruginosaGraph 3: Zone of Inhibition at 0.3 ml Turmeric oil
B.cereus B.subtilis E.coli
S.aureus S.epidermis S.albus
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Results
Figure 9 : 0.4 ml Turmeric OilGraph 4: Zone of Inhibition at 0.4 ml Turmeric oil P.aeruginsoa
S.aureus S.epidermis S.albus
B.cereus B.subtilis E.coli
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Results
Figure 10: 0.5 ml Turmeric Oil Graph 5: Zone of Inhibition at 0.5 ml Turmeric oil
B.cereus B.subtilis E.coli
S.aureus S.epidermis S.albus
P.aeruginosa
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Results Inhibition zone increases as the concentration of
turmeric oil increases for gram positive bacteria. But little or no bacterial inhibition on gram
negative bacteria. P.aeruginosa
No inhibition zone
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Results
Figure 11 : 0.1 ml Turmeric powder Graph 6: Zone of Inhibition at 0.1 ml Turmeric powder
B.cereus B.subtilis E.coli
S.aureus S.epidermis S.albus
P.aeruginosa
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Results
Figure 12: 0.2 ml Turmeric powder Graph 7: Zone of Inhibition at 0.2 ml Turmeric powder
P.aeruginosa
S.aureus S.epidermis S.albus
E.coliB.subtilisB.cereus
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Results
Figure 13: 0.3 ml Turmeric powder Graph 8: Zone of Inhibition at 0.3 ml Turmeric powder
B.cereus B.subtilis E.coli
S.aureus S.epidermis S.albus
P.aeruginosa
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Results
Figure 14: 0.4 ml Turmeric powder Graph 9: Zone of Inhibition at 0.4ml Turmeric powder
B.cereus B.subtilis E.coli
S.aureus S.epidermis S.albus
P.aeruginosa
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Results
Figure 15: 0.5 ml Turmeric powderGraph 10:Zone of Inhibition at 0.5 ml Turmeric powder
B.cereus B.subtilis E.coli
S.aureus S.epidermis S.albus
P.aeruginosa
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Results Used Oneway ANOVA in SPSS to statistically
analyze the results. There is statistically a significant difference in
bacterial inhibition among different types of bacteria (gram +/gram -) using turmeric oil. 0.000, 0.006, 0.002 (p-value<0.05)
There is statistically no significant difference in bacterial inhibition among different types of bacteria (gram +/ gram -)using turmeric powder. 0.779, 0.066, 0.004 (p-value >0.05)
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Results It is also analyzed that the effect of oil and powder
extracts is noticeable more in gram positive bacteria than in gram negative bacteria.
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Discussion One study done in Nepal presented that antibacterial
activity of Curcuma longa is “more prominent on the Gram positive bacteria than the Gram negative bacteria .”2
Because of Difference in sensitivity 2
Difference in morphological organization in microorganisms 2
Another past study stated that Curcuma Longa aqueous extract possessed good antimicrobial activity at low concentration.3
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Acknowledgement Dr. Dorothy Anthony Vicki Stanavitch Christine Paraise Jason Koval Dr. Patricia Miller KC Security
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References 1. Alavijeh, P. K., Alavijeh, P. K., & Sharma, D. (2012). A study of antimicrobial
activity of few medicinal herbs. Asian Journal of Plant Science and Research, 2(4), 496-502. ISSN: 2249-7412
2. Chanda, S., & Baravalia, Y. (2010). Screening of some plant extracts against some skin diseases caused by oxidative stress and microorganisms. African Journal of Biotechnology, 9(21), 3210-3217. ISSN: 1684–5315
3. Niamsa, N., & Sittiwet, C. (2009). Antimicrobial activity of curcuma longa aqueous extract. Journal of Pharmacology and Toxicology, 4(4), 173-177. ISSN:1816-496X
4. http://www.mnn.com/sites/default/files/Turmeric_main_0127.jpg5. http://www.anandaapothecary.com/oil-im/turmeric.jpg6. http://www.turmeric.co.in/images/turmeric_plant.gif7. http://www.fishersci.com/ecomm/servlet/fsproductdetail_10652_633906_29104_-1_08. http://www.fishersci.com/ecomm/servlet/fsproductdetail?
productId=632328&catCode=RE_SC&&storeId=10652#