Figures removed due to copyright reasons. Please see: Fig ...€¦ · Using Bimodular Polyketide...
Transcript of Figures removed due to copyright reasons. Please see: Fig ...€¦ · Using Bimodular Polyketide...
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Figures removed due to copyright reasons.
Please see: Fig.2A-D in Top Curr Chem 209 (2000): 1-51.
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Figure removed due to copyright reasons. Please see Fig. 1 in Current Opinion in Chemical Biology 7 (2003): 285-295.
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chain length factor (CLF)--> reduces carbonyl to hydroxyl
like dehydratasesCYC and ARO very similar --> same type of protein
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Figure removed due to copyright reasons.
Please see Fig. 44 in Nat Prod Rep 18 (2001): 380-416.
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KR / reduction
changes way PK folds on itself
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Figure removed due to copyright reasons. Please see Fig. 1 in JACS 125 (2003): 12708-12709.
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self cleavage from the thioester (see novobiocin biosynthesis from NRPS section)
K
KR --> each KR has own regioselectivity --> timing of KR. --> sometimes before ARO/CYC
sometimes acts after ARO/CYC
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Figure removed due to copyright reasons.
Please see Fig. 11 in Top Curr Chem 209 (2000): 1.
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Figure courtesy of: Tang, Yi, Taek Soon Lee, and Chaitan Khosla.
"Engineered Biosynthesis of Regioselectively Modified Aromatic Polyketides Using Bimodular Polyketide Synthases." PLoS Biology 2, no.2 (2004): 0227-0238.
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* = 14C (radioisotope)
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starting material
"Primary metabolism"immediate precursor
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Figure removed due to copyright reasons. Please see Fig. 8 in Nat Prod Rep 18 (2001): 380-416.
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Content removed due to copyright reasons. Please see: Mann, John. Chemical Aspects of Biosynthesis. New York, NY: Oxford University Press, 1994, pp. 22-23, 26-27.
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Figure removed due to copyright reasons. Please see Fig. 23 in Nat Prod Rep 18 (2001): 380-416.
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Figure removed due to copyright reasons.
Figure removed due to copyright reasons.
Please see Scheme 1 in J Biol Chem 275 (2000): 38393-38401.
Please see Scheme 1 in ChemBioChem 4 (2003): 713-715.
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Figure removed due to copyright reasons.Please see Fig. 6-13 in Nat Prod Rep 18 (2001): 380-416.