Figure I. FPLC FXN 29 33 34 35 36 29 33 34 35 36 + D-4F Sc D-4F STOKES DIAMETER nm 17 12.2 10.4 8.2...
10
Figure I. FPLC FXN 29 33 34 35 36 29 33 34 35 36 + D-4F Sc D- 4F STOKES DIAMETER nm 17 12.2 10.4 8.2 7.1 HDL PEAK HDL PEAK C C P C C P 30K
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Transcript of Figure I. FPLC FXN 29 33 34 35 36 29 33 34 35 36 + D-4F Sc D-4F STOKES DIAMETER nm 17 12.2 10.4 8.2...
Figure I.
FPLC FXN 29 33 34 35 36 29 33 34 35 36
+ D-4F Sc D-4F
STOKESDIAMETER
nm17
12.210.48.27.1
HDLPEAK
HDL PEAKC C P C C P
30K
Figure II.
F irst di mension
Seco
nd
dim
en
sion
Water Control + D-4F
9.5
8.2
7.1
[n m ]
Pre-beta HDL Pre-beta HDL
0.0
5.0
10.0
15.0
20.0
25.0
30.0
35.0
40.0
20 30 400.0
10.0
20.0
0.0
5.0
10.0
15.0
20.0
25.0
30.0
35.0
40.0
20 30 400.0
10.0
20.0
CH
OLES
TER
OL,
mg
r /
DL
CH
OLES
TER
OL,
mg
r /
DL
PO
N A
CTIV
ITY
, U
/ m
L
PO
N A
CTIV
ITY
, U
/ m
L
FRACTION # FRACTION #
CHOLESTEROLPON ACTIVITY
CHOLESTEROLPON ACTIVITY
Sc D-4F + D-4F
Figure III.
MIGRATED MONOCYTES
PERFIELD
0
2
4
6
8
10
12
14
0
2
4
6
8
10
12
14
No Addition
Control
LDL+
hHDL
Assay Controls
50 µg HDL Cholesterol
10 µg CCP Cholesterol
P=0.01
P=0.01
16
LDL+
D-4F CCP
LDL+
Chow Control CCP
LDL+
D-4F HDL
LDL
Chow Control HDL
LDL+
Figure IV.
Figure V.
0
5
10
15
20
25
30
35
Control human
HDL
Sc D-4F D-4F
% C
hole
ste
rol Effl
ux
from
Macro
ph
ag
es
0.1% BSA
P<0.01
Murine HDL, 50 g
LOOHPer
Cholesterol
A B C
D E
LOOHPer
Cholesterol
D-4FChow
Pre-Beta HDL *
0.00 0.00
D-4F
0.00
Chow
FPLC Fractions after Pre-beta HDL
0
1.5
3.0
4.5
6.0
0
1.5
3.0
4.5
6.0
Sc-D-4F D-4FChow0
2
4
6
8
10
12 VLDL/IDL
*
Sc -D-4F D -4FChow
LDL
*
-D 4FChow
HDL
ScD-4F
0
2.0
1.0
0.5
1.5
2.5
0
2
4
6
8
10
12
14
1614
*
Figure VI.
Sc-D-4F Sc-D-4F
0
10
20
30
40
50
60
70
P
ON
un
its
Water Control
Sc D-4F D-4F
*
Figure VII.
Scrambled D-4F D-4F
Pre-beta Pre-beta alpha-HDL alpha-HDL
First Dimension
Secon
d d
imen
sio
n
Figure VIII.
F irst di mensio nS
eco
nd
dim
en
sion
Water Control + D-4F
8.2
7.1
[n m ]
Pre-beta HDL Pre-beta HDL
Figure IX.
Control, Chow
D-4FControl, Chow
D-4F
ng
LO
OH
per
well
*
*
Pre-Beta HDL
HDL
0
500
1000
250
750
1250
Figure X.
