Fig. S3. Potential HGT region donors -...

RB1 5-end C1 to C2, 18618 bp; MGAS23530_0120 - 0142M49_NZ131

M87_NGAS743

M82_NGAS596

M5_Manfredo

M18_MGAS8232

M6_MGAS10394

M53_Alab49

M59_MGAS15252

M2_MGAS10270

M89_MGAS23530

M89_MGAS11027

M14_HSC5

M4_MGAS10750

M?_ATCC19615

M6_JRS4M6_D471

M83_NGAS327

M83_7F7M83_STAB1102

M28_M28PF1M28_MGAS6180

M89_MGAS27061

M1_NCTC8198

M1_SF370M1_AP1

M59_MGAS1882

M3_SSI-1M3_STAB902

M3_MGAS315

0.0010

M1_5448

M1_MGAS5005

M1_MTB313M1_MTB314

M1_A20M1_476

M44_STAB901M44_1E1

M12_HKU16M12_MGAS9429M12_MGAS2096

M12_HKU360

A

RB1 FCT C1 to C2, 13525 bp; MGAS23530_0143 - 0152

M82_NGAS596 M87_NGAS743

M89_MGAS11027

M49_NZ131

M?_ATCC19615M18_MGAS8232

M53_Alab49

M5_Manfredo

M4_MGAS10750

M2_MGAS10270

M14_HSC5

M12_HKU16M12_MGAS9429

M12_HKU360

M12_MGAS2096

M6_D471M6_JRS4M6_MGAS10394


M44_STAB901M44_1E1

M59_MGAS15252M59_MGAS1882

M89_MGAS23530M89_MGAS27061

M3_STAB902M3_SSI-1M3_MGAS315

M83_NGAS327M83_7F7

M83_STAB1102

M1_5448

M1_SF370

M1_476M1_MGAS5005

M1_MTB313M1_MTB314

M1_A20

M1_NCTC8198

M1_AP10.1

B

M89_MGAS27061


M5_ManfredoM18_MGAS8232

M14_HSC5

M?_ATCC19615

M53_Alab49

M89_MGAS11027


M82_NGAS596

M28_MGAS6180M28_M28PF1

M87_NGAS743M4_MGAS10750

M49_NZ131

M83_7F7

M83_NGAS327M83_STAB1102

M6_D471M6_MGAS10394

M6_JRS4

M12_HKU16M12_HKU360


M44_1E1M44_STAB901

M3_STAB902

M3_MGAS315M3_SSI-1

0.0010

M1_476M1_MGAS5005

M1_MTB313M1_MTB314

M1_A20M1_5448

M1_SF370M1_AP1 M1_NCTC8198

RB1 3-end C1 to C2, 39631 bp; MGAS23530_0153 - 0191

C

RB2 C1 to C2, 2094 bp; MGAS23530_0315 - 0316

M89_MGAS27061

M2_MGAS10270

M89_MGAS11027

M89_MGAS23530

M12_HKU360M87_NGAS743

M82_NGAS596

M?_ATCC19615

M14_HSC5

M1_SF370

M1_AP1

M1_NCTC8198

M1_MTB314M1_MTB313M1_5448

M83_NGAS327

M53_Alab49

M4_MGAS10750M23_M23ND

M5_Manfredo

M18_MGAS8232

M49_NZ131

M12_HKU16M12_MGAS9429M12_MGAS2096


M6_JRS4M6_D471M6_MGAS10394

M44_STAB901M44_1E1


M1_MGAS5005

M1_476M1_A20

M83_STAB1102M83_7F7

M3_STAB902

M3_SSI-1M3_MGAS315

0.0010

D

RB3-4 C1 to C2, 27172bp; MGAS23530_0786-0812

M89_MGAS27061M89_MGAS23530



M53_Alab49M14_HSC5

M?_ATCC19615

M49_NZ131

M2_MGAS10270M87_NGAS743


M82_NGAS596

M12_HKU16M12_HKU360


M3_STAB902M3_SSI-1M3_MGAS315


M44_1E1M44_STAB901

M1_SF370M1_5448

M1_MGAS5005M1_A20

M1_476

M1_MTB313M1_MTB314

M1_AP1M1_NCTC8198


M83_NGAS327M83_STAB1102M83_7F7

0.0010

E

RB5 C1 to C2, 39037bp; MGAS23530_0930-0970

0.0010

M89_MGAS11027


M18_MGAS8232

M4_MGAS10750M5_Manfredo

M2_MGAS10270

M89_MGAS27061M89_MGAS23530

M82_NGAS596

M?_ATCC19615M53_Alab49

M87_NGAS743M49_NZ131

M14_HSC5 M12_HKU16

M12_MGAS2096M12_MGAS9429M12_HKU360

M3_MGAS315M3_STAB902

M3_SSI-1

M6_JRS4

M1_SF370

M1_5448

M1_476

M1_MGAS5005

M1_MTB313

M1_A20

M1_MTB314

M1_AP1M1_NCTC8198

M44_STAB901M44_1E1


M83_7F7

M6_D471



F

Fig. S3. Potential HGT region donors

RB6 C1 to C2, 7789 bp; MGAS23530_1027 - 1032

G


M?_ATCC19615

M87_NGAS743M53_Alab49

M49_NZ131

M23_M23ND

M82_NGAS596

M14_HSC5M89_MGAS11027



M89_MGAS27061

M44_STAB901, M44_1E1

M83_STAB1102M83_NGAS327

M83_7F7

M5_ManfredoM1_A20M1_476

M1_MGAS5005M1_NCTC8198M1_AP1

M1_MTB314

M1_MTB313

M1_SF370

M1_5448


M3_SSI-1M3_STAB902M3_MGAS315

M6_MGAS10394M6_JRS4

M6_D471


M12_MGAS2096M12_HKU360

0.0010

RB7 C1 to C2, 17515 bp; MGAS23530_1532 - 1544

H

M5_Manfredo

M14_HSC5

M89_MGAS11027M89_MGAS23530


M49_NZ131

M87_NGAS743M82_NGAS596

M53_Alab49 M?_ATCC19615

M6_MGAS10394

M6_D471M6_JRS4

M23_M23ND

M18_MGAS8232

M12_HKU16M12_HKU360M12_MGAS2096M12_MGAS9429

M1_A20

M1_MTB313

M1_MTB314M1_SF370

M1_AP1M1_476

M1_NCTC8198

M1_MGAS5005

M1_5448

M83_NGAS327M83_STAB1102M83_7F7


M44_STAB901M44_1E1


M89_MGAS27061

M3_SSI-1M3_STAB902M3_MGAS315

0.01

RB9 C2 to C3, 1764 bp; MGAS23530_0118 - 0119

J

M2_MGAS10270


M18_MGAS8232 M5_Manfredo

M23_M23ND

M89_MGAS27061M59_MGAS15252M59_MGAS1882

M87_NGAS743

M14_HSC5

M?_ATCC19615M49_NZ131

M83_NGAS327

M1_NCTC8198M1_SF370M1_AP1M1_5448M1_MGAS5005M1_MTB313M1_MTB314

M1_A20M1_476

M53_Alab49

M12_2096, M12_9429, M12_HKU16, M12_HKU360M6_MGAS10394

M83_STAB1102M83_7F7

M44_1E1M44_STAB901

M6_JRS4M6_D471

M3_SSI-1M3_STAB902

M3_MGAS315


0.0010

M89_MGAS23530M89_MGAS11027

I

RB8 C1 to C2, 19532 bp; MGAS23530_1561 - 1581


M5_Manfredo

M18_MGAS8232

M87_NGAS743M2_MGAS10270

M53_Alab49

M82_NGAS596


M59_MGAS1882

M44_STAB901

M?_ATCC19615

M14_HSC5M83_NGAS327M83_7F7

M83_STAB1102

M4_MGAS10750

M49_NZ131


M12_MGAS9429

M44_1E1

M59_MGAS15252

M89_MGAS23530M89_MGAS27061

M6_JRS4M6_MGAS10394

M6_D471 M3_STAB902

M3_SSI-1M3_MGAS315

0.0010

M1_MTB314M1_SF370

M1_A20

M1_NCTC8198

M1_5448

M1_MGAS5005

M1_MTB313

M1_AP1

M1_476

RB11 C2 to C3, 352 bp; MGAS23530_0265

L

M82_NGAS596

M49_NZ131



M23_M23NDM2_MGAS10270

M87_NGAS743

M89_MGAS23530

M4_MGAS10750M6_MGAS10394M18_MGAS8232M89_MGAS11027

M6_JRS4M6_D471

M44_STAB901M44_1E1

0.0010

M1_A20

M1_MTB314M1_NCTC8198M1_476M1_SF370

M1_MTB313M1_MGAS5005

M1_AP1M1_5448

M83_7F7

M12_HKU16

M59_MGAS1882

M?_ATCC19615


M12_MGAS2096

M12_HKU360

M12_MGAS9429

M14_HSC5

M3_MGAS315M3_SSI-1M3_STAB902

M53_Alab49

M59_MGAS15252

RB10 C2 to C3, 10407 bp; MGAS23530_0173-0183

K

M49_NZ131

M2_MGAS10270

M89_MGAS23530

M18_MGAS8232

M5_ManfredoM23_M23ND

M82_NGAS596

M14_HSC5M?_ATCC19615

M4_MGAS10750

M89_MGAS11027

M53_Alab49

M89_MGAS27061

M12_HKU16


M12_MGAS9429

M6_JRS4

M6_MGAS10394M6_D471

M1_NCTC8198

M1_SF370M1_AP1

M3_MGAS315

M3_SSI-1M3_STAB902

M83_7F7M83_STAB1102M83_NGAS327


M87_NGAS743


M44_STAB901M44_1E1

0.01

M1_5448M1_476

M1_MGAS5005

M1_MTB314

M1_MTB313

M1_A20

, M1_476


RB13 C2 to C3, 801 bp; MGAS23530_1543 - 1544

N

RB12 C2 to C3, 764 bp; MGAS23530_1464

M

0.01

M12_HKU16M82_NGAS596


M?_ATCC19615

M44_STAB901

M14_HSC5 M87_NGAS743M53_Alab49

M5_Manfredo

M18_MGAS8232



M44_1E1

M3_SSI-1

M3_STAB902M3_MGAS315

M89_MGAS23530M89_MGAS11027

M1_5448M1_476

M1_AP1M1_MGAS5005

M1_MTB313M1_MTB314

M1_SF370

M1_NCTC8198

M1_A20

M6_JRS4M6_D471M6_MGAS10394M89_MGAS27061

M23_M23ND

M12_MGAS2096


M83_7F7M49_NZ131

M83_STAB1102M83_NGAS327

O

RB14 C2 to C3, 1193 bp; MGAS23530_1670 - 1671

0.001

M18_MGAS8232

M4_MGAS10750

M23_M23ND

M12_MGAS9429 M87_NGAS743 M82_NGAS596


M2_MGAS10270


M49_NZ131

M3_SSI-1M3_MGAS315

M3_STAB902

M44_STAB901M44_1E1

M12_MGAS2096

M12_HKU360M12_HKU16

M89_MGAS23530M89_MGAS11027

M?_ATCC19615M83_STAB1102

M14_HSC5M53_Alab49M83_7F7M83_NGAS327

M89_MGAS27061

M6_D471M6_JRS4

M6_MGAS10394M5_Manfredo

M1_MGAS5005

M1_476M1_AP1

M1_MTB313M1_A20

M1_5448M1_MTB314M1_NCTC8198M1_SF370

RB15 C3 to SC3D, 17818 bp; MGAS23530_0365 - 0385

P

M89_MGAS28980

M87_NGAS743

M89_MGAS23530

M18_MGAS8232

M49_NZ131

M12_MGAS2096

M12_HKU360M12_HKU16

M4_MGAS10750

M2_MGAS10270

M82_NGAS596

M?_ATCC19615

M5_Manfredo


M59_MGAS15252

M53_Alab49

M6_JRS4M6_D471M6_MGAS10394

M3_STAB902M3_MGAS315M3_SSI-1

M83_STAB1102M83_7F7M83_NGAS327

M12_MGAS9429

M89_MGAS11027

M44_1E1M44_STAB901

M59_MGAS1882

M14_HSC5

M23_M23ND

M1_MTB314

M1_476M1_NCTC8198M1_AP1M1_MTB313

M1_SF370

M1_5448

M1_A20M1_MGAS5005

0.01

M89_MGAS27061

0.001

M23_M23ND

M18_MGAS8232

M5_Manfredo

M89_MGAS27061

M82_NGAS596


M14_HSC5

M2_MGAS10270

M44_1E1M44_STAB901

M53_Alab49

M4_MGAS10750

M1_A20M1_476M1_5448M1_MGAS5005M1_AP1M1_MTB313M1_MTB314M1_NCTC8198M1_SF370M3_MGAS315M3_SSI-1M3_STAB902

M49_NZ131

M?_ATCC19615


M83_7F7


M12_HKU360M89_MGAS11027

M12_MGAS2096M12_MGAS9429M12_HKU16


M6_JRS4

M6_MGAS10394M6_D471

Fig. S3. Potential horizontal gene transfer (HGT) region donors. Shown for each of the predicted recombi-

nation blocks (RB) separating the clades are the genetic relationships among the three emm89 clade refer-

ence strains and 39 strains of 18 other emm types for which there are complete genome sequences publi-

cally available as of July 10, 2015. Sequences flanking the predicted recombination blocks in the strain

MGAS23530 genome were used to define the corresponding regions in the other strains using blastn. The

sequences corresponding to the predicted recombination blocks among all 42 strains were aligned using

MAFFT, and relationships were inferred by neighbor-network split-decomposition using SplitsTree. The

length of the recombination block and locus tags of the genes involved are listed relative to strain

MGAS23530. Of note, seven of the eight recombination blocks separating all 359 clade 1 strains from all

78 clade 2 strains share a more recent common ancestor with emm2 reference strain MGAS10270 than

with reference strains of any of the other emm-types used in this comparison.


Fig-S3_donors-1.pdfFig-S3_donors-2.pdfFig-S3_donors-3.pdf

Fig. S3. Potential HGT region donors -...

Documents

Transcript of Fig. S3. Potential HGT region donors -...