Fig. 1-29. Pg. 25 Inherited Disorders “Sporadic” Cancers Aging Lysosomal Storage diseases I-Cell...
55
Fig. 1-29
-
Upload
evangeline-quinn -
Category
Documents
-
view
217 -
download
0
Transcript of Fig. 1-29. Pg. 25 Inherited Disorders “Sporadic” Cancers Aging Lysosomal Storage diseases I-Cell...
Fig. 1-29
Pg. 25
Inherited Disorders“Sporadic” CancersAging
Lysosomal Storage diseasesI-Cell Disease
Cystic FibrosisCholera
“Sporadic” CancersAging
Alzheimer’sDisease
Cystic Fibrosis
MuscularDystrophy
Drug Targets
Microtubules
Membrane receptors
Translation componentstRNA synthetases
Ribosomes
Ion channels
Protein kinases
G proteins
Bcl-2 Family proteins
Telomerase
Transport vesicles
Protein “pumps”
Topoisomerases
Pg. 10
100 µM
Microscopy
Light Microscopy
Electron Microscopy
Pg. 10
Light Microscopy
Bright Field
Fluorescence
Phase-Contrast
Differential Interference Contrast (DIC)
Electron Microscopy (EM)
Scanning EM
Transmission EM
Chemical Composition of Cells
Pg. 58
Pg. 79
Chemical Bond Energies
Gene Expression
Monomer/Polymer
Nitrogenous Bases of DNA and RNA
Pg. 76
Pg. 175
3’
3’
5’
5’
0.34 nm
Pg. 171
Major Groove
Minor Groove
Topoisomerase Treated DNA
Topoisomerase Relieves Stress of OverwindingDuring DNA Replication
Camptothecin
Topoisomerase is an Anti-Cancer Drug Target
Werner Syndrome
15 yrs. 48 yrs.
Mutation in gene encoding RecQ helicase
Human Karyotype
Pg. 179
247 M bp
47 M bp
Nucleus
~ 2 meters of DNA fits in the nucleus!
Nitrogenous Bases of DNA and RNA
Pg. 76
RNA Molecules
5’ 5’
3’3’
Types of RNA Molecules
mRNA (gene expression)
Transfer RNA (Translation)
Ribosomal RNA (Translation)
Small nuclear RNA (exon splicing)
Micro RNA (Gene regulation)
Small cytoplasmic RNA (protein transport)
Ribozymes (numerous biochemical functions)
Protein FunctionsEnzymesMembrane transportIntercellular transportHormonesReceptorsNanomachinesAntibodiesToxinsFibersBioluminescence/Fluorescence
Pg. 74
(Pgs. 74-75)
Amino Acid Side Chains
Extra carbon atom!
ß-Sheet
alpha-helix
Hydrophobic Interactions in Tertiary Structure
Hydrogen Bonding in Tertiary Structure
Representations of Protein Tertiary Structure
Backbone
Ribbon
Wire Frame
Space Filling
Pg. 132
Quaternary Structure
Pg. 141
Protein Domains
(Src protein)
Chloride Ion Channel Protein
Comparison of Protein Primary Structure
Cyclin-dependent Kinase Proteins
(cytosol or ER membrane)
Complexity of Human Gene Expression
Pg. 202 (Modified for RNA synthesis)
RNASynthesis
OH
OH
OH
Pg. 233
1 µm
Pg. 233
Exon/Intron Organization of Eukaryotic Genes
Pg. 238
Sequence Structure of Introns
Pg. 239
Pg. 241
Alternative Splicing
Pg. 237
5’ Guanine Cap Structure
