Field of View 4X

Field of View 10X

Field of View 40X

4X

10X

40X

Depth of Field – the vertical distance of an object that is in focus. When magnification is increased, less of the object is in focus (depth of field decreases) – BUT greater detail of the area in focus can be seen.

Depth of Field

Not in focus

In focus

Stage Micrometer

Ocular Micrometer

Stage Micrometer = 10 units =10*0.01mm = .1mm

Ocular Micrometer = 9 units

1 ocular division = .1mm / 9 units = 0.011mm

Each small division = 0.011 mm

This cell spans across 11 ocular divisions. How wide is this cell? 11*0.011=0.121mm.

CytoplasmNucleus

Plasma membrane

Generic Animal Cell

Eukaryotic – With a membrane bound nucleus

Animal cell anatomy

Plant cell anatomy

Flagellum

Cell WallCytoplasm

Plasma Membrane

Ribosome

Nucleoid Region

Generic Prokaryote Cell

Prokaryotic – Without a membrane bound nucleus