Importance of Blastocyst Morphology in Selection for Transfer
Fertilization, Maternal recognition of pregnancy and … › wp-content › uploads › 2020 › 04...
Transcript of Fertilization, Maternal recognition of pregnancy and … › wp-content › uploads › 2020 › 04...
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Fertilization, Maternal recognition of pregnancy and Implantation
• Prof G N Purohit
Head Department of Veterinary Gynecology and Obstetrics, College of Veterinary and Animal Science, Bikaner, Rajasthan, India.
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Some definitions
• Obstetrics Science of midwifery (obstare "stand opposite to)
• A branch of medicine that deals with care of pregnant women during pregnancy, at child birth and afterwards
• Veterinary Obstetrics:
• A branch of Veterinary Medicine that deals with
• care of animals during pregnancy, at parturition
• and during the postpartum period
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• Embryo: from fertilization to the differentiation stage (45 days in cattle)
• Fetus: from differentiation till parturition• Conceptus: Fetus with its fetal membranes.• Nullipara: An animal which has not become pregnant and not
parturated.• Primipara: An animal which has become pregnant and would
parturate for the first time• Monotocous(Uniparous): Animals giving birth to single young ones• Polytocous : Animals giving birth to many young ones• Embryology: Science of physiological development of the fetus• Teratology: Embryology + pathology dealing with abnormal fetal
development•
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Carazol: a beta-blocker given to sows to reduce the expulsive time of labor in sows.Clenbuterol: A beta-adrenergic that quietens the uterus and inhibits labor.Psuedopregnancy: False pregnancy occurs in the bitch and the goat and in many other species like mare.Feathering: Tapping of the vagina of a whelping bitch with finger leads to oxytocin release and delivery of a pup.Single pup syndrome: The presence of a single pup in a bitch leading to problem in initiation of parturition and dvelopment of an extra large puppy.Spalding sign: Overlapping of the fetal skull bones seen on radiography of a bitch with dead fetuses.Fergusons reflex: The release of huge quantities of oxytocin in cows when the fetal legs touches thee pelvis during parturition.Theilers disease: Infectious hepatitis in mare developing due to injection of an eCG.
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PREGNANCY IN DOMESTIC ANIMALS
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• The period of intrauterine development (from fertilization to parturition) in mammals istermed pregnancy or gestation.
• Ovum + sperm fertilization Embryo
Completion of gestation Growth in uterus
(Pregnancy) Parturition
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FERTILIZATION
• External Amphibians
• Internal Mammals
• Fertilization in mammals
– Gamete transport
– Changes in gamete structure
– Cleavage and blastocyst formation
– Maternal recognition of pregnancy
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Gamete transport
• Synchronous transport of male and female gametes to oviduct ampulla should occur
• Semen deposited in vagina (human,
• cattle, buffalo, sheep, goats, rabbits).
• Semen deposited in the uterus
• (in dogs, horses, pigs).
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Oocyte transport
• Except mares the oocyte is picked by the fimbriae and enters the ostium
• Propelled towards the ampulla by ciliated cells
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Species Fertile life
sperm (hrs)
Fertile life
ovum
(hrs)
Day of
entry of
embryo in
uterus
Blastocyst
formation
Cattle 24-48 20-24 3-3.5 7-8
Horse 72-120 6-8 4-5 6
Sheep 30-48 16-24 3 6-7
Swine 24-72 8-10 2 5-6
Dog 120-168 48-72 5 7-8
Buffalo 24-32 6-12 4 5-6
Fertile life of gametes in domestic animals
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Changes in gamete structure
• Sperm capacitation, sperm oocyte interaction, acrosomereaction, oocyte activation, zona reaction and intermingling of male and female gametes
• Capacitation occurs in female tract
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Effect of CapacitationEpididymal Ejaculated
SeminalPlasmaand/or
EpididymalSecretions
+
Capacitated
FemaleTract+
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Zona Pellucida
PerivitellineSpace
Oocyte
Ca2+
Ca2+
Ca2+
Ca2+
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Zona Pellucida
PerivitellineSpace
Oocyte
Ca2+
CorticalGranules
Zona Block to Polyspermy
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Sperm acrosomal
region binds to sperm
receptor on zona
Sperm penetrates
zona
Initiates -
Zona &
Vitelline
Reaction
Initiates completion of
Meiosis II and release
of 2nd polar body
Zona and Vitelline
Reactions block
polyspermy
Perivitelline
Space
Vitelline
Membrane
Cortical
GranuleZP3
Binding
Initiation of
Acrosome
Reaction
Continuation of
ReactionAcrosome-Reacted
SpermPenetration
Excluded Sperm
Zona
Reaction
Cortical
Granule
Release
Fusion
1st Polar BodyZona
Pellucida
Redrawn from
Wasserman, P.M. 1988 Scientific American. p78
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Na+ [Na+]
H2O
Zona Pellucida
Trophectoderm
Inner Cell Mass
Blastulation
Tight Junctions
Gap Junctions
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Hatching
• Bovine
– 9 - 11 days
• Equine, Ovine
– 7 - 8 days
• Porcine
– 6 days
Buffalo
6.5 days
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Conceptus Growth
Spherical
Filamentous
Cow
• Day 15, 1-2 mm
• Day 18-19, 10-20 cm
Tubular
Embryonic Area
ElongatingTrophoblast
Mare remains spherical!
Occurs in cow, pig and sheep
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Elongated Day 15 Porcine Conceptus
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Uterine Location of Elongating Ruminant Blastocyst
CorpusLuteum
Bovine and Ovine
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Pig Intrauterine Migration
Day 5
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Pig Intrauterine Migration
Day 7
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Pig Intrauterine Migration
Day 12
Embryos become fixed
Transuterine migration is rare in cow and ewe!
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Trans-uterine Migration in the Mare
CorpusLuteum
Fixation on day 15 - 16
Fixation can occur in either horn!
Begins Day 10
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Gastrulation
BlastocoeleCavity
Endoderm
Inner Cell Mass Trophectoderm
Formation of Germ Layers
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Gastrulation
EndodermEndoderm
Yolk Sack
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Gastrulation
EndodermEndoderm
Yolk Sack
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GastrulaEctoderm Mesoderm
Yolk Sack
Endoderm Trophectoderm (Chorion)
ExtraembryonicCoelom
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Germ Layers• Ectoderm
– CNS
– Sense organs
– Mammary glands
– Sweat glands
– Skin
– Hair
– hooves
• Mesoderm
– Circulatory
– Skeletal
– Muscle
– Reproductive tracts
– Kidneys
– Urinary ducts
Endoderm» Digestive
» Liver
» Lungs
» Pancreas
» Thyroid gland
» Other glands
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Fetal membrane Formation
Yolk Sack
Chorion
EmbryonicEctoderm
Mesoderm
Trophectoderm
Amniotic Folds
Extraembryonic Ceolom
Endoderm
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Extraembryonic Ceolom
Amniotic FoldAmniotic Cavity
Yolk Sack
Allantois
Chorion
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Yolk Sack
Extraembryonic Ceolom
Chorion
AmnionAmnionic Cavity
Carnuncle
Allantois
UterineLumen
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Chorion
Allantochorion(Chorioallantois)
Allantois Cavity
Cotyledon
Amniotic Cavity
Amnion
YolkSack
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Fetal (Placental) Membranes
• Yolk Sack– In birds to nourish embryo
– In mammal atrophies but source of blood cells and primordial germ cells
• Amnion– Non-vascular, fluid filled
– Fluid produced by fetus
– Protective cushion
– Ruptures at birth (not breaking of water)
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Placental Membranes
• Allantois
– Blood vessels
– Fuses with chorion
• Allantochorion or chorioallantois
• Brings blood vessels to chorion
• Chorion
– Outermost membrane
– Attachment to mother
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Maternal Recognition of Pregnancy
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Luteal Regression
PGF2a
(Equine)
No Uterine Factor(human)
PGF2a
(Bovine, Ovine,Porcine)
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CL Status
CL
(+)Uterine PGF2a
Filamentous Embryo(+)
Maintained
CAMechanisms
CL Regression•Luteolytic•No CL support
CL Maintenance•Antiluteolytic
» Block PGF2a effects•Luteotrophic
» Stimulate progesterone production by CL
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Recognition of Pregnancy in Bovine and Ovine
Conceptus Secretions• Interferons
•oIFN-t•bIFN-t
Mechanism• Antiluteolytic
• Inhibit oxytocin receptor synthesis• Inhibit PGF2a synthesis
Critical Days• Bovine - 16 to 17• Ovine - 12 to 13
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Recognition of Pregnancy in the PorcineConceptus Secretions
• Estradiol
Critical Days
11 to 12
Mechanism
•Antiluteolytic
» Redirect PGF2a
» Requires 2 embryos per horn
PGF2a
PGF2a
PGF2a
PGF2a
E2
E2
E2
E2
E2
E2
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Recognition of Pregnancy in the Mare
PGF2aPGF2a
Conceptus Secretion• ?
Critical Days10 to 14
Mechanism• antiluteolytic
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Recognition of Pregnancy in the Human
HCG
Conceptus Secretes
• HCG
Mechanism
• luteotrophic
Critical Days•8 to 12
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Dog and Cat
• CL does not regress if not pregnant
– no uterine effect
• Psuedopregnancy
– CL last similar length as in pregnancy
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Implantation
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Time of ImplantationAttachment to Uterus
Species Beginning Completion
pig 13 24
cow 22 40
sheep 15 28
mare 24 - 40 95 - 100
human 7 - 8 30
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Species Time (days )
Cattle 17 to 35
Sheep 14 to 18
Pigs 12 to 16
Horses 17 to 56
Dogs 14 to 18
Cats 12 to 14
Human 6 to 7
The time from ovulation, during which implantation occurs
in different species.
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Type of Implantation
• Centric– Chorion lies opposed to uterine wall
– Farm animals
• Eccentric– Chorionic sack in a uterine fold
– Rodent
• Interstitial– Embryo digests part of uterine wall
– human
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