Fertilization a process at the beginning of ourselves.
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Transcript of Fertilization a process at the beginning of ourselves.
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Fertilizationa process at the beginning of ourselves
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•Sperm A human sperm. •Egg Overview The zona pellucida.
Structure of Sperm and Egg
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Scanning electron micrograph of a human sperm contacting a hamster egg. For detail
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Scanning electron micrograph of a clam egg with
sperm bound to its surface.
For detail
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Block to polyspermy
• A depolarization of the egg plasma membrane a rapid, primary block to polyspermy
• Egg cortical reaction a slow, secondary block to polyspermy
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primary block to polyspermy
• Na+ influx triggered by fusing with sperm make the membrane potential of egg shift from –60mV to +20mV within a few second.
• Low concentration of Na+ raise the incidence of polyspermy. Artificially depolarized an unfertilized egg prevent sperm from fusing with egg.
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primary block to polyspermy
• It seems likely that the depolarization alter the conformation of a crucial protein in the egg plasma menbrane so that the sperm membrane can not fuse with the egg membrane.
• The egg membrane potencial returns to normal within a few minutes after fertilization.
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• a second mechanism must provide a long-term barrier to polyspermy.
• In most egg, including those of mammals, this barrier is provided by Cortical Reaction.Back
Therefore
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Second block to polyspermy
• When the sperm fuses with the egg plasma membrane, it activates the inositol phospholipid cell-signaling pathway in the egg. This, in turn, causes a local increase in cytosolic Ca2+, which spreads through the cell in a wave. The rise in Ca2+ activate the egg and initiate the cortical reaction.
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Second block to polyspermy
• cortical granules located just under the plasma membrane of the egg release their contents by exocytosis. A,B
• The enzymes released by the cortical reaction change the structure of the zona pellucida, which becomes hardened so that sperm no longer bind to it, thereby providing a slow, secondary block to polyspermy. End
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A mature Xenopus egg, ready for fertilization. For detail
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The zona pellucida
The zona pellucida. For detail
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A human sperm. It is shown in longitudinal section
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Drawing of the midpiece of a mammalian sperm
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Depolarization &
Cortical reaction
are the mechanics of
Block to Polyspermy
continue
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Acrosome Reaction
• Sperm penetrate the zona pellucida by acrosome reaction.
• Process of acrosome reaction. Back
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Process of Fertilization
• Acrosome reaction• Deplarization• Cortical Reaction• 2nd oocyte complete Meiosis Ⅱ• Pronucleus• Replication of DNA in pronucleus• First Mitosis
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