Faculty of Biological Sciences - Jihoesk univerzita

Research and study programmes 2004/5 – biennial information prospectus

University of South Bohemia

in České Budějovice, Czech Republic

Faculty of Biological Sciences

Biennial information prospectus for everyone interested in collaboration with or the study of biology and ecology

at the Faculty of Biological Sciences October 2006

České Budějovice – the old downtown

Faculty of Biological Sciences – building “B”

... come to discover the world of nature

Research and study programmes 2004/5 – biennial information prospectus

University of South Bohemia

in České Budějovice, Czech Republic

Faculty of Biological Sciences

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For updated information, see www-pages:

http://www.bf.jcu.cz

“This material is issued by the University of South Bohemia in České Budějovice, with the financial support of the European Community Commission within the framework of the Socrates Programme. The publication is the responsibility of the publisher himself and does not necessarily represent the views of the EU Commission or its services and offices.”

Faculty of Biological Sciences University of South Bohemia in České Budějovice, Czech Republic

Research and study programmes at Faculty of Biological Sciences 2004–2005



Published in October 2006

by Faculty of Biological Sciences, University of South Bohemia in České Budějovice, Czech Republic Typesetting and Design by academic officers and co.

Printed by: WIP Reklama spol. s r.o.

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Preface This biennial information prospectus is meant to serve as an initial information and reference source for anyone interested in collaboration with the Faculty of Biological Sciences, the University of South Bohemia in České Budějovice, Czech Republic, or in the study of biology and ecology. Any such material is limited by two factors: its extent and the time of publication. It was not possible to include all relevant information and we are aware of the changes which will arise after issue due to the development of our institution. Thus, we have to ask the reader to look for further and more regularly updated information on the www pages of our Faculty at the address given on the previous page and – if more information is required – to use the e-mail addresses of the Faculty officers and ask them directly. The www pages also serve to inform the reader of the formalities of arranging long-term residence permits for foreign students, accommodation details, living costs, and other matters of interest to a person visiting or staying in the Czech Republic. Authors Organized by František Sedláček; numerous people from various departments of the Faculty collected data for this material. The help given by Petr Kos and Christopher Steer from the Language Department is greatly appreciated as well as the help of Oldřich Nedvěd with typographic corrections.

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Contents

Preface ........................................................................................................ 3 Contents ...................................................................................................... 4 About the university .................................................................................. 5 About the faculty ....................................................................................... 7 Collaborating scientific institutes .......................................................... 15 Departments ............................................................................................. 19

Department of Animal Physiology ..................................................... 19 Department of Botany ......................................................................... 23 Department of Ecology and Hydrobiology ....................................... 28 Department of General Biology ......................................................... 34 Department of Genetics ...................................................................... 38 Department of Mathematics, Physics and Chemistry ..................... 44 Department of Molecular Biology and Biochemistry ...................... 46 Department of Parasitology ............................................................... 50 Department of Plant Physiology and Anatomy ................................ 57 Department of Zoology ....................................................................... 61 Language Department ........................................................................ 66

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Basic information about the University The University of South Bohemia is a public educational and research institution of the university type, with five faculties offering a selection of tertiary education programmes. Rector´s Office: Branišovská 31, CZ-370 05 České Budějovice, Czech Republic Prof. PhDr. Václav Bůžek, CSc. (Rector) Prof. RNDr. Zdeněk Brandl, CSc. (Vice-Rector, Education and Student Affairs) Prof. PaedDr. Vladimír Papoušek, CSc. (Vice-Rector, External and Foreign Relations) Prof. RNDr. Jan Zrzavý, CSc. (Vice-Rector, Research) Prof. Ing. František Střeleček, CSc. (Vice-Rector, Development) Doc. PhDr. Jana Šemberová, CSc. (Vice-Rector, Lifelong Learning) Ing. Hana Kropáčková (Bursar) Dr. Ing. Alois Křišťan, Th.D. (Academic Senate, Chairman) Number of Students: 9,290 Total number of staff: 852 Faculties Faculty of Agriculture Dean: Prof. Ing. Magdalena Hrabánková, CSc. Address: Studentská 13, CZ-370 05 České Budějovice, Czech Republic http://www.zf.jcu.cz/ Faculty of Biological Sciences Dean: Prof. RNDr. Libor Grubhoffer, CSc. Address: Branišovská 31, CZ-370 05 České Budějovice, Czech Republic http://www.bf.jcu.cz/ Faculty of Health and Social Studies Dean: Prof. MUDr. Miloš Velemínský, CSc. Address: Jírovcova 24, CZ-370 04 České Budějovice, Czech Republic http://www.zsf.jcu.cz/ Pedagogical Faculty Dean: Prof. RNDr. Miroslav Papáček, CSc. Address: Jeronýmova 10, CZ-371 15 České Budějovice, Czech Republic http://www.pf.jcu.cz/ Philosophical Faculty (from January 1, 2006) Dean: PhDr. Miroslav Novotný, CSc. Address: Na Mlýnské stoce 35, CZ-370 01 České Budějovice, Czech Republic http://www.ff.jcu.cz/

Theological Faculty Dean: Doc. Dr. Jiří Kašný, Th.D. Address: Kněžská 8, CZ-370 01 České Budějovice, Czech Republic http://www.tf.jcu.cz/

University Institutes Institute of History (till December 31 2005) Director: PhDr. Miroslav Novotný, CSc. Address: Jeronýmova 10, CZ-371 15 České Budějovice, Czech Republic http://www.hu.jcu.cz/ Research Institute of Fish Culture and Hydrobiology Director: Doc. Ing. Otomar Linhart, DrSc. Address: Zátiší 728/II, CZ-389 25 Vodňany, Czech Republic http://www.vurh.jcu.cz/ Institute of Physical Biology Director: RNDr. Dalibor Štys, CSc. Address: Zámek Nové Hrady, Zámecká 136, CZ-373 33 Nové Hrady, Czech Republic http://www.ufb.jcu.cz/

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History and characteristics of the University of South Bohemia The University was established by Act of Parliament in September, 1991, as a part of the system of public university institutions. At the very beginning, the new university consisted of just two faculties, the Pedagogical Faculty and the Faculty of Agriculture, that had previously existed independently in the town from the middle of the 20th century. Soon after this, in November 1991, the University Academic Senate decided to establish three more, including the Faculty of Biological Sciences. The original two faculties are still the largest components of the university, while the other three are of the size of approximately one tenth of the total student number each.

The University does not cover all possible fields of tertiary education. It is focused on the education of teachers, specialists in agriculture and its management and economics, non-physician staff of medical and health-care institutions, people in various religious services – and biologists. The future plans of the university include the enlargement of this selection to include humanities and further branches of science. The University offers study programmes at the bachelor, master and doctoral levels. The accredited doctoral programmes are in Biology, Agriculture and Agricultural Management, History, History of Czech Literature, and Theology. The University is located in the regional centre of the South Bohemia Region, the town of České Budějovice (population approx. 100 000), about 150 km south of Prague. The town was established in the thirteenth century at the confluence of the river Vltava and its tributary the Malše where the Czech King Přemysl Otakar II founded a “King’s town”. The wisdom of the King’s choice of location can still clearly be seen while walking around the large central square of the town. During its history, the town was a crossroads of commerce not only in the region but also on trade routes from the European South to Central Europe. The first European horse-drawn railway connected the town with Linz in Austria in 1832. Further industrial development (pencils and beer) stimulated the growth of the city and turned it into a regional centre.

Three of the faculties of the University are located in the old part of the town while the Faculties of Agriculture and of Biological Sciences are placed in the ever growing university campus on the western edge of the town. Besides these two faculties the campus comprises of student residences, a Rector’s office and also the buildings of the Biological Institutes of the Academy of Sciences of the Czech Republic.

The University includes not only the six faculties but also some other institutions, such as the European Information Centre, the University Training Farm, etc. Of special interest to the biologist are the Research Institute of Fish Culture and Hydrobiology, and the Institute of Physical Biology. The former is located in the near-by town of Vodňany, surrounded by carp ponds which are a typical component of the South-Bohemian countryside. The research programme of this institute includes basic and applied research in aquaculture, namely genetics, reproduction and the breeding of carp and other pond-cultivated fish, fish-farming technologies, and the ecology and ecotoxicology of water environment.

The Institute of Physical Biology in Nové Hrady village is a scientific institution focused on basic and applied research including the development of new technologies and devices concerning the physiology of biomembrane processes during photosynthesis in plant cells. Besides research and development activities, the institute offers a number of short-time, highly specialized postgraduate courses for graduate students and postdoctoral scientists involving numerous teachers from other countries.

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Basic information about the Faculty In 1991, scientists from the Academy of Sciences of the Czech Republic (AS CR) established the Faculty of Biological Sciences (BF JU) – see organization chart, figure 1. The faculty became a crucial element responsible for the establishment of the University of South Bohemia. It has served as a base for education and training in biological disciplines within the framework of accredited programmes. At first, only undergraduate programs were provided by the faculty. Later, the undergraduate programs were naturally extended to graduate and post-graduate levels, respectively. Despite administrative separation, close links between the faculty and institutions of the AS CR have been maintained. This association is demonstrated in figure 2, which shows the existing organizational structure of the faculty. Scientists from the AS CR are, to a large extend, involved in teaching and they also supervise Ph.D. studies. The BF JU and corresponding institutions of the AS CR mutually share accreditation for Ph.D. programs.

Fig. 1: Organizational chart of the Faculty of Biological Sciences

Academic Senate RNDr. Roman Fuchs, CSc. (chairman) Mgr. Pavel Hrouzek (vice-chairman) Mgr. Tomáš Hauer RNDr. Jan Kaštovský, Ph.D. Mgr. Roman Kuchta Prof. RNDr. Jan Š. Lepš, CSc. Mgr. Simona Poláková Bc. Pavel Říha Prof. Ing. Hana Šantrůčková, CSc. Ing. Milan Štech, Ph.D. Mgr. Eva Uhlířová, Ph.D.

Scientific Board Prof. RNDr. Zdeněk Brandl, CSc. Prof. RNDr. Václav Hypša, CSc. Doc. RNDr. Marek Jindra, CSc. Prof. RNDr. Jan Š. Lepš, CSc. Prof. RNDr. František Marec, CSc. Prof. RNDr. Karel Prach, CSc. Prof. Ing. Hana Šantrůčková, CSc. Prof. Ing. Miloslav Šimek, CSc. Prof. RNDr. Jan Zrzavý, CSc.

external members RNDr. Jiří Hejnar, CSc. Prof. RNDr. Milan Kodíček, CSc. Doc. Ing. Jan Krekule, DrSc. Doc. Ing. Petr Ráb, DrSc. Prof. RNDr. Jan Šmarda, CSc. Prof. RNDr. Jan Zima, DrSc.

Dean Prof. RNDr. Libor Grubhoffer, CSc.

Vice-Dean, Student Affairs

Doc. RNDr. Šárka Klementová, CSc.

Vice-Dean, Research and

Ph.D.-programmes Prof. Ing. Miloslav

Šimek, CSc.

Secretary of the Faculty

Ing. Alena Kamišová

Vice-Dean, Development

Doc. RNDr. Petr Šmilauer, Ph.D.

Vice-Dean, Foreign Relations

Doc. RNDr. František Sedláček,

CSc.

Individual departments (structure on page 16)

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Research Research at the faculty is mainly focused on ecology and biology. The profile is, however, systematically modified in accordance with the concept of “integrative biology”. This concept puts strong emphasis on the evolutionary aspects of biology and ecology. To gain insight into the dynamic development of living systems model systems are studied at all levels, from whole populations to organisms and molecules.

The Faculty of Biological Sciences is formally divided into departments, however collaboration among researchers in these departments is maintained, providing important flexibility and the transfer of knowledge between otherwise historically separated fields. The faculty has gained international respect in the 15 years of its existence, mainly due to support from national as well as international grant agencies. In addition, there has also been significant support from local grant agencies to individual research groups. All grants and research proposals submitted to grant agencies support the long-term development and integration of research performed by the faculty. Research performed at the faculty is predominantly oriented towards the biodiversity.

Biodiversity is one of the most remarkable features of life on earth; from the variety of molecular mechanisms that underlie living functions to the number of alleles in a population, from the morphological variety of organisms to the diversity of ecosystems on Earth. Human society is also dependent on the stability and functioning of the ecosystem. However, we change and threaten the functioning of ecosystems. The study of biodiversity and general biological factors, and the relationship between biodiversity and ecosystem functioning, has recently become a priority scientifically and politically. The ability to predict the behaviour of organisms to a changing environment and how the environment also is shaped by these changes in biodiversity and the functions of organisms is a major task of biological sciences.

The list of problems linked to the modern biodiversity crisis indicates the necessity to focus on the application of molecular approaches. Phylogenetic reconstruction today inevitably needs the identification and sequencing of appropriate genes, which requires specific genomic knowledge. Nowadays, application of molecular methods is widespread in the study of population, ecological and applicable conservation issues. The development of molecular biology as a tool for studying biodiversity is occurring by widening the spectra of model organisms and also further, intensive research on the functional and structural genomics of traditional models (Trypanosoma, Drosophila, Caenorhabditis). Concrete problems under study at the faculty could be divided into five domains as follows:

1) Biodiversity and functional ecology of communities and ecosystems

• Relation between biodiversity and function of plant communities and the mechanisms of species diversity.

• Interaction and equilibrium between basic functional types in field communities. • Functional relationships between soil and field communities • Economic utilisation of plant sources and soil microorganisms. • Analysis of the direction, velocity and causes of seral changes and the impact of

invasive species on various seral stages. • Interaction, function and stability of water community organisms. • Modelling life strategies and growth of organisms, populations and communities.

2) Biodiversity: from systematic biology to wildlife protection • Tropical ecology. • Biodiversity in the Czech Republic and its protection. • Synantropisation, synurbisation and fragmentation of terrestrial vertebrate

populations.

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3) Phylogeny and evolutionary biology of organisms: the origin of biodiversity • Phylogeny of Metazoa. • Phylogeny and evolution of the ecology of insects. • Phylogeny and evolution of the biology of vertebrates.

4) Biodiversity and evolution of ecological association and social systems • Parasitism and symbiosis. • Mimicry. • Anti predatory behaviour and intra-specific aggression of vertebrates.

5) Evolutionary genomics of model organisms • Functional genomics of trypanosomes. • Functional genomics of transcription factors in nematodes, fruit flies and new insect

models. • Study of the control of growth and development in fruit flies. • Structural genomics: Study of molecular mechanisms in trypanosomes and inhibitor

development.

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Teaching The Faculty of Biological Sciences offers programmes at all three levels: Bachelor's, Master's and Ph.D. studies. Above all the Doctoral and partly the Master's levels may be of interest to foreign students. Bachelor's Programmes The three-year Bachelor's Programmes should supply a student with the basic knowledge necessary either for subsequent master's studies or for practical professional employment. Students choose either “Introductory Biology Programme” (preparation for further studies in Biology or Biology for teaching) or “Biophysics”, or some professionally orientated programmes – “Environmental Care” or “Biomedical Laboratory Technology”. These programmes are conducted in the Czech language only, although the students are required to achieve a knowledge of English sufficient to allow them to study from English biological textbooks. Master's Programmes The Master's Programmes are open to any student who has successfully finished a Bachelor's programme in biology or related fields at any university. Applicants are accepted on a competitive basis, depending on their results in the admission examination. They may enter different programmes – “Biophysics”, “Botany”, “Clinical Biology”, “Ecology”, “Experimental Biology”, “Parasitology”, and “Zoology”. Most of these programmes are conducted in Czech only, some also in English and the number of English versions is expected to grow. Students are meant to gain more theoretical knowledge in their field of specialization but should also produce a diploma thesis which should result in a paper to be published in the appropriate regular international scientific journal. A good command of English is a skill which students must obtain during this programme. Due to the growing interest in exchange programmes for students (such as Socrates/ Erasmus) in Europe or even further afield, the programmes offer foreign students a set of half-year courses and within those courses a number of interconnected topics. Doctoral Programmes The intention of the doctoral programmes is education for highly qualified scientific and research work. The main duty of Ph.D. students is seen in individual work on their theses. During the study programme, which lasts 3 years, the students enlarge their knowledge in a specific field of study. They attend recommended lectures and courses and also are involved in the teaching of undergraduate students. Each year the students have to give a seminar or talk focused on the methodology (first year) and the results of their own experimental work. The students are encouraged to present the results at international conferences). The study is completed by passing the final examinations and by the defence of the student’s doctoral thesis. It is desirable that the thesis comprises of published and accepted papers in recognized international journals.

The student is supervised by a thesis advisor, who is responsible fort the appropriate scientific content of the work. The title Doctor of Philosophy (Ph.D.) in a given area of specialization is awarded to successful graduates. A citizen of any country is eligible for enrolment if he/she meets the requirements for the selected area of specialisation, is able to meet the financial obligations related to the studies – tuition fees and residency requirements in the Czech Republic.

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Biology – specialization Entomology Programme Director: Doc. RNDr. Oldřich Nedvěd, CSc. Study specialization in entomology offers the training of students in basic entomological disciplines and their general scientific basis with respect to the modern field and laboratory methods and laboratory equipment. All the thesis advisors are actively working in the field in question and most of them are distinguished specialists in entomology. Subjects of Ph.D. theses are related to both general and/or theoretical research in (arachno-) entomology, i.e. morphology, physiology, genetics, systematics and taxonomy, and phylogeny, as well as to applied research.

Areas of Ph.D. research: Relations of herbivores and plants in the tropical rain forest, and in the temperate forest. Metapopulation ecology and mobility of butterflies. Taxonomy of selected insect groups. Relations of diapause and cold/heat/drought resistance in arthropods. Aphidophagous insects and their use in biological control. Biology – specialization Hydrobiology Programme Director: Prof. RNDr. Karel Šimek, CSc. The doctoral programme in hydrobiology assures the training of students in various areas of current limnology, dealing mainly with the production, microbial and chemical processes and nutrient transformation in reservoir and lake ecosystems and their watersheds. In the scope of the programme, one can select specific topics of fundamental research of different aspects of the functioning of reservoir and lake ecosystems. Alternatively, an holistic approach and other approaches suitable in applied research and water management area are taught. Specific aspects of fundamental research can be solved by means of the cultivation of organisms under controlled conditions in simplified model systems. Special attention is paid to the acquisition of proper experimental and monitoring skills, supporting the students’ ability to appropriately plan experiment and devise sampling strategies and statistical evaluations.

Areas of Ph.D. research: Nutrient resources in watersheds and their transport by streams and rivers to reservoirs and lakes. Chemistry and microbiology of sediments in reservoirs and lakes – importance for nutrient cycles. Mathematical modelling of hydrodynamics, eutrophication, and water chemistry in reservoirs. Feeding biology of fish, spatial distribution of fish in reservoirs and lakes, interaction of fish-zooplankton. The zooplankton population structure in relationship to environmental conditions in lakes and reservoirs. Trophic relationships within predaceous crustacean zooplankon. Life strategy and migration of the genus Daphnia. Ecological interactions in reservoir and lake phytoplankton communities. Extracellular enzyme activities in a water environment: ecological significance, resources, kinetics. Factors shaping bacterial dynamics and community composition in lakes and reservoirs. Food-web interactions of pelagic protists with bacteria and phytoplankton. Biology – specialization Parasitology Programme Director: Prof. MVDr. Iva Dyková, DrSc. This study program offers training in the basic fields of parasitology. The lectures and laboratory assignments are given by instructors engaged in parasitological research. Dissertation topics involve fundamental research in the biology of parasitic organisms and their host relations and the area of medical and veterinary parasitology.

Areas of Ph.D. research: Human parasitoses: protozoan infections in immunodeficient patients, host-parasite relation including the immune response. Protozoan and myxosporean parasites in fish: morphology, life cycles and pathogenicity. Amphizoic amoebae – distribution in fish, pathogenicity and molecular phylogeny. Biology, ecology and diversity of all principal groups of helminths parasitic in fish. Molecular biology and biochemistry of parasites: phylogenetic relationships of selected groups of protists based on the analysis of

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ribosomal RNA genes; structure of mitochondrial DNA in various groups of protists. Molecular ecology of blood sucking vectors in relation to mechanisms of pathogen transmission: protein-saccharide interactions, immune reactions in disease vectors. Botany – specialization Botany Programme Director: Prof. RNDr. Jan Š. Lepš, CSc. Education of students in all basic botanical disciplines including the problems of both cryptogams and vascular plants. Lectures are given by specialists experienced in various branches of botanical science, who usually have long research experience and who participate in projects and scientific programmes at the Faculty of Biological Sciences of the University of South Bohemia, and/or at the Institute of Botany of the Academy of Sciences. The themes of dissertations represent a wide spectrum of botanical research. Several projects are closely connected with the core programme of Czech botanists, i.e. the elaboration of the complete Flora of the Czech Republic, but other projects are broadly concerned with important hydrobiological, ecological or landscape ecological problems. Several areas of study focus on the problems of speciation, diversification, and modern molecular taxonomy of cyanobacteria, algae, bryophytes and vascular plants. The latest modern laboratory (genetic, physiological) and field methodological procedures (population biology, ecological and autecological approach) are used for this work; the methods aimed at the investigation of the ecology of plant communities, succession, invasive plants, the role of plants in ecosystems and in landscape are an integral part of the educational procedures. Students participate in several important Czech or international research projects.

Areas of Ph.D. research: Taxonomy and ecology of cyanobacteria, algae and vascular plants and their speciation, diversification and function in ecosystems. Population ecology, dynamics and variability in population development. Fytogeography and chorology of higher plants. Geobotany. Co-existence and competition of species in plant communities. Invasive plants. Vegetation succession. Archeobotany and palynology. Ecology – specialization Ecology Programme Director: Prof. Ing. Hana Šantrůčková, CSc. The programme is focused on the principles and methods of contemporary ecology, with special attention paid to the mechanisms of ecosystem functions. The interactions among plants and animals, in both aquatic and terrestrial ecosystems are considered, with emphasis on the evolutionary consequences of ecological phenomena. Experimental approaches are emphasised, with courses on the design of ecological experiments and analysis of ecological data. Ecological theory is dealt with in several courses.

Areas of Ph.D. research: Population ecology of selected species. Community ecology of plants and animals. Theoretical ecology and mathematical modelling of ecological systems. Mechanisms of species coexistence. Plant roots and their symbionts. Community ecology of soil organisms. Carbon and nitrogen cycling in soil and ecosystem. Greenhouse gas production in and emissions from soil. Soil biological processes and their impact on soil fertility/quality. Molecular and Cell Biology – specialization Molecular and Cell Biology and Genetics Programme Director: Doc. RNDr. Marek Jindra, CSc. Independent thinking, good English, and motivation to strive to discover new things, even at the expense of one’s own comfort, are the main prerequisites to succeed in this programme. Students are guided toward independent scientific research in the fields of molecular, cell and developmental biology. They need to master molecular and genetic methods of work with diverse model organisms ranging from protists and yeast to plants and animals. Student

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projects are secured by supervisors active in international research. Topics of Ph.D. theses are derived from the aims of grant projects and rely on high-tech equipment as well as on international collaboration; most students experience a short assignment in a foreign partner’s laboratory. Their projects typically aim to clarify some of the fundamental issues of current biology, such as those concerned with the regulation of cell functioning or differentiation. Students are also trained in molecular cytogenetics, gene engineering and animal and plant genetics; special attention is devoted to the techniques of genetic modifications.

Areas of Ph.D. research: Structure of proteins and nucleic acids. Function of mitochondrial proteins, mechanisms of transcription and RNA editing in parasitic protists (Trypanosoma, Leishmania). Evolution of the telomere structure. Sex chromosomes. Transcription factors and regulation of gene expression. Cell cycle and proliferation (growth factors, oncogenes and tumour suppressors). Development and differentiation (Drosophila melanogaster and Caenorhabditis elegans models). Molecular basis of photosynthesis. Genes encoding silk. Molecular mechanism of circadian rhythms. Transgenic approaches in plants and animals. Functional genomics of plant viruses. Repetitive sequences in eukaryotic genomes. Development of DNA chips. Physiology and Immunology – specialization Anatomy and Physiology of Plants Programme Director: Doc. Ing. Jiří Šantrůček, CSc. The Ph.D. course offers advanced education in several important branches of plant physiology and anatomy. It is supported by the experience of supervisors, renewed scientists working at the Institutes of the Academy of Sciences of the Czech Republic, employing new biochemical and biophysical methods and hi-tech equipment in plant physiology, anatomy and ecophysiology projects. Subjects of the doctoral theses are focused on various topics of the photochemistry of photosynthesis in higher plants, algae and cyanobacteria, on the structural and functional aspects of biomembranes and on interactions of the leaf and the whole plant with the environment (environmental and stress plant biology). The graduates will be competent in a broad scale of plant science research, agricultural, forestry, horticultural and biotechnology programs and should have advanced skills in scientific communication.

Areas of Ph.D. research: Photosystem (PS) II studies. Light induced degradation of PS II proteins. Structure and function of small PS II proteins. Adaptation of algae to abiotic stress. Fractioning of stable isotopes in photosynthesis and in plant metabolism. Function and structure of plant cuticle. Spatial heterogeneity of leaf and plant photosynthesis. Plant carbon economy and optimization of stomatal function. Physiology and Immunology – specialization Animal Physiology and Developmental Biology Programme Director: Prof. RNDr. Ladislav Janský, DrSc. The animal physiology and developmental biology study discipline provides schooling in various general and specialised physiological fields. Basic research at molecular, cellular or organismic levels from the environmental aspect is being performed on insect, vertebrate and human models. Thesis advisors are well known scientists working actively in the field with the help of modern methodology. Students learn to use up-to-date methods of physiology, biochemistry, endocrinology, cell biology and immunology using computerised data acquisition systems and are trained to present their data at scientific meetings.

Areas of Ph.D. research: Regulations and mechanisms of metabolic adaptations to cold in insects and mammals. Physiological basis of cold hardiness. Adaptations of anaerobic metabolism of fish. Cold adaptation in man. Physiological and immunological mechanisms of fever. Regulatory mechanisms controlling insect diapause and mammalian hibernation. Mode of action of brain-gut peptides at cellular and molecular levels. Insect hormones – control of

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secretion. Insect hormone agonists and antagonists – practical aspects. Effect of plant protective compounds on nutrient utilisation of phytophagous insects. Zoology – specialization Zoology Programme Director: Prof. RNDr. Jan Zrzavý, CSc. The Ph.D. program provides instruction in basic zoological disciplines, supervised by thesis advisors who are active zoological and ecological research workers able to apply modern methods and laboratory equipment. The topics of Ph.D. theses include problems and methodology of modern biology and ecology of animals, especially animal phylogeny, tropical ecology, host-parasite coevolution, arthropod morphology and systematics, bird ecology and behaviour, mammal ecology, neuroethology and sociobiology, and fish ecology and phylogeny.

Areas of Ph.D. research: Phylogeny of selected animal taxa (e.g. Arthropoda, Vertebrata). Experimental and evolutionary analysis of insect warning coloration and its relationship to bird predation. Influence of fluctuating temperatures on the development and survival of the ectotherm animals. Population and feeding ecology of fish. Phylogeny and historical ecology of the selected fishes (Cichlidae). Nesting biology, feeding ecology and habitat preferences of selected bird species (Falconidae, Laridae, Passeriformes). Behaviour and sociobiology of fossorial mammals (Arvicolidae, Bathyergidae). Sense ecology and neuroethology of small mammals.

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Collaborating scientific institutes of the Academy of Sciences of the Czech Republic Five institutes of the Academy of Sciences of the Czech Republic located in České Budějovice and grouped into the Biology Centre as well as five other institutes participate in organising the doctoral study programmes (see Fig. 2). Biology Centre AS CR Director: Vít Našinec, CSc. Address: Branišovská 31, CZ-370 05 České Budějovice, Czech Republic http://www.bc.cas.cz/ Hydrobiological Institute Director: Doc. RNDr. Josef Matěna, CSc. Address: Na Sádkách 7, CZ-370 05 České Budějovice, Czech Republic http://www.hbu.cas.cz/ Institute of Entomology Director: Doc. RNDr. Jan Šula, CSc. Address: Branišovská 31, CZ-370 05 České Budějovice, Czech Republic http://www.entu.cas.cz/ Institute of Parasitology Director: Prof. RNDr. Tomáš Scholz, CSc. Address: Branišovská 31, CZ-370 05 České Budějovice, Czech Republic http://www.paru.cas.cz/ Institute of Plant Molecular Biology Director: Doc. Ing. Josef Špak, DrSc. Address: Branišovská 31, CZ-370 05 České Budějovice, Czech Republic http://www.umbr.cas.cz/ Institute of Soil Biology Director: RNDr. Václav Pižl, CSc. Address: Na Sádkách 7, CZ-370 05 České Budějovice, Czech Republic http://www.upb.cas.cz/ Institute of Animal Physiology and Genetics, AS CR Director: Doc. MVDr. Ivan Míšek, CSc. Address: Rumburská 89, CZ-277 21 Liběchov, Czech Republic http://www.iapg.cas.cz/ Institute of Botany, AS CR Director: Doc. RNDr. Jan Kirschner, CSc. Division of Plant Ecology in Třeboň Address: Dukelská 135, CZ-379 82 Třeboň, Czech Republic http://www.butbn.cas.cz/ Institute of Microbiology, AS CR Director: Prof. RNDr. Blanka Říhová, DrSc. Division of Autotrophic Microorganisms at Třeboň Address: Opatovický mlýn, CZ-379 81 Třeboň, Czech Republic http://www.biomed.cas.cz/mbu/autotroph.html Institute of Systems Biology and Ecology, AS CR Director: Prof. RNDr. Ing. Michal V. Marek, DrSc. Address: Na Sádkách 7, CZ-370 05 České Budějovice, Czech Republic http://www.usbe.cas.cz/ Institute of Vertebrate Biology, AS CR Director: Prof. RNDr. Jan Zima, DrSc. Address: Květná 8, CZ-603 65 Brno, Czech Republic http://www.ivb.cz/

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Fig. 2 Relations of the Faculty to Academy Institutes

Departments Collaborating scientific institutes (numbers indicate intensive connections) of the Academy of Sciences (accredited for doctoral programmes)

Dept. of Botany

Dept. of Ecology and Hydrobiology

Dept. of Genetics

Dept. of Plant Physiology and Anatomy

Dept. of Animal Physiology

Dept. of Molecular and Cell Biology

Dept. of General Biology

Dept. of Parasitology

Dept. of Zoology

Dept. Mathematics, Physics, Chemistry

3, 5, 9

1, 3, 4, 5, 8

4, 6, 7

4, 7

4

4, 6

4, 6, 10

3, 7

1, 2, 4, 6, 10

4

Language Department Joint divisions of the faculty and collaborating scientific institutes of the Academy of Sciences Laboratory of Molecular Taxonomy Laboratory of Electron Microscopy Laboratory of Sequential Methods Library

1 - Hydrobiological Inst.

2 - Inst. of Animal Physiology and Genetics

3 - Inst. of Botany

4 - Inst. of Entomology

5 - Institute of Microbiology

6 - Inst. of Parasitology

7 - Inst. of Plant Molecular Biology

8 - Inst. of Soil Biology

9 - Inst. of Systems Biology and Ecology

10 - Inst. of Vertebrate Biology

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Lecture hall of institutes of the Academy of Sciences

Training in microscopy

The Laboratory of Electron Microscopy

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Joyful pipetting in the Laboratory of Molecular Taxonomy

Field course of sea invertebrates

Hypselodoris – an amazing capture

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Departments Staff – Scientific activities – Study programmes – Selected publications

Department of Animal Physiology

Staff Doc. RNDr. Dalibor Kodrík, CSc. (Head) – [email protected] – insect physiology, insect

neurohormones, protein purification

Prof. RNDr. Pavel Blažka, CSc. – [email protected] – physiology of aquatic animals

Prof. RNDr. Ladislav Janský, DrSc. – [email protected] – human physiology, physiology

and temperature

Prof. RNDr. František Sehnal, CSc. – [email protected] – insect endocrinology,

developmental biology

Prof. RNDr. Jan Žďárek, DrSc. – [email protected] – insect etology and endocrinology

Doc. RNDr. Magdalena Hodková, CSc. – [email protected] – insect ecophysiology,

chronobiology

Doc. Ing. Vladimír Košťál, CSc. – [email protected] – insect diapause, cryobiology

Doc. RNDr. Václav Němec, CSc. – [email protected] – insect physiology, insect

endocrinology

Doc. RNDr. František Sedláček, CSc. – [email protected] – mammal neuroethology

Doc. RNDr. Stanislav Vybíral, CSc. – [email protected] – human physiology, physiology

and temperature

RNDr. Ivo Hodek, CSc. – [email protected] – insect ecophysiology, photoperiodism

RNDr. Blanka Kalinová, CSc. – [email protected] – insect neurophysiology

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Scientific activities The Department of Animal Physiology deals with (1) insect physiology, (2) human

physiology and (3) the physiology of fish.

Insect physiology is focused on insect endocrinology. The insect physiology research

group deals with insect metabolic and stress hormones – adipokinetic peptides from the

AKH/RPCH family. The group recently isolated and characterised two new adipokinetic

octapeptides from the insect Pyrrhocoris apterus corpora cardiaca and is now studying their

function in the insect’s body. It was found that topical application and/or injection of the

peptide induces lipid mobilisation, but is inactive in the mobilisation of carbohydrates.

Application of the hormones also stimulates insect locomotion. The ELISA test revealed that

the whole AKH content fluctuates during the development of the adult life of the bug and also

within a 24-hour period. The diel changes of AKH content in CNS are correlated with diel

changes of adipokinetic response and also with locomotor activity. In addition, significant

differences of AKH content among macropterous, brachypterous and diapausing adults were

recorded.

Another insect physiology research group studies changes in the content of proteins,

glycogen, neutral lipids and sterols in locust eggs from oviposition to hatching. The amount of

proteins increased progressively during embryogenesis, while glycogen and lipids were

successively metabolised or utilised for the development of the embryo.

Human physiology research group deals with the physiological mechanisms of cold

adaptation and fever. It was found that whole body exposure to cold water induces a decrease

in rectal temperature, while the local peripheral cooling of lower extremities does not

influence rectal temperature and metabolic rate, but increases heart rate and brain

temperature. The group also analysed dynamics in the activity of different thermoregulatory

outputs and changes in the functions of thermoregulatory centres after peripheral

administration of cytokines.

The fish physiology research group studies primarily anaerobic metabolism in the anoxia

resistant C. carassius and in the wild form of C. auratus (silver crucian carp). The ammonia

excretion rate in anoxia at 5°C is significantly lower than suggested previously and

corresponds evidently to the catabolism of damaged proteins. Current studies deal with

mitochondrial anoxia, the role of CO2 in anaerobic metabolism and the variability of

anaerobic end products under different environmental conditions.

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Study programmes The Department of Animal Physiology co-ordinates study programmes and student theses in

animal physiology. The department is responsible for the master study programme

“Experimental Biology” – specialisation: “Animal Physiology” and Ph.D. study programme

“Physiology and Immunology” – specialisation: “Physiology and Developmental Biology of

Animals” (guarantees: D. Kodrík and P. Blažka).

Selected publications of the department

2004 Begum M., Breuer M., Kodrík D., Rahman M.M., DeLoof A., 2004. The NMDA receptor antagonist MK-801

inhibits vitellogenesis in the flesh fly Neobellieria bullata and in the desert locust Schistocerca gregaria. Journal of Insect Physiology 50: 927–934.

Hagele B.F., Wang F.H., Sehnal F., Simpson S.J., 2004. Effects of crowding isolation and transfer from isolation

to crowding on total ecdysteroid content of eggs in Schistocerca gregaria. Journal of Insect Physiology 50: 621–628.

Janský L., Stará T., Matějovská T., 2004. Humoral modulation of cytokine release from human peripheral blood

mononuclear cells (PBMC). Physiological Research 53: 13. Janský V., Vybíral S., 2004. Thermal homeostasis in systemic inflammation: modulation of neuronal

mechanisms. Frontiers in Bioscience 9: 3068–3084. Košťál V., Tamura M., Tollarová M., Zahradníčková H., 2004. Enzymatic capacity for accumulation of polyol

cryoprotectants changes during the diapause development in the adult red firebug Pyrrhocoris apterus. Physiological Entomology 29: 344–355.

Košťál V., Tollarová M., Šula J., 2004. Adjustments of the enzymatic complement for polyol biosynthesis and

accumulation of diapausing cold-acclimated adults of Pyrrhocoris apterus. Journal of Insect Physiology 50: 303–313.

Košťál V., Vambera J., Bastl J., 2004. On the nature of pre-freeze mortality in insects: water balance ion

homeostasis and energy charge in the adults of Pyrrhocoris apterus. Journal of Experimental Biology 207: 1509–1521.

Lorenz M.W., Zemek R., Kodrík D., Socha R., 2004. Lipid mobilisation and locomotor stimulation in Gryllus

bimaculatus (de Geer) (Ensifera, Gryllidae) by topically applied adipokinetic hormone. Physiological Entomology 29: 146–151.

Rakouský S., Ondřej M., Sehnal F., Habuštová O., Hussein H.M., Ovesná J., Kučera L., Kocourek F., Říha K.,

Dostálová R., Seidenglanz M., Tejklová E., Griga M., 2004. Transgenic plant products and their introduction into the environment and to crop protection systems, a risk assessment. In: Nap, J.-P., Atanassov, A., Stiekema, W. J. (eds.): Proc. NATO Advanced Res. Workshop Genomics for Biosafety in Plant Biotechnology, Bansko, Bulgaria, 15–19 October, 2004, NATO Science Series: Life and Behavioural Science, 359. IOS Press, Amsterdam, The Netherlands, pp. 173–184.

Romanovsky A.A., Steiner A.A., Barczayova L., Janský L., Gourine V.N., 2004. Arginine vasopressin in fever:

a still unsolved puzzle. Journal of Thermal Biology 29: 407–411.

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Roth J., Vybíral S., Zeisberger E., Janský L., 2004. Endogenous antipyretics: Neuropeptides and glucocorticoids. Frontiers in Bioscience 9: 816–826.

Sehadová H., Markova E.P., Sehnal F., Takeda M., 2004. Distribution of circadian clock-related proteins in the

cephalic nervous system of the silkworm, Bombyx mori. Journal of Biological Rhythms 19: 466–482.

Sehnal F., Žurovec M., 2004. Construction of silk fiber core in Lepidoptera. Biomacromolecules 5: 666–674. Socha R., Kodrík D., Šimek P., Patočková M., 2004. The kind of AKH-mobilized energy substrates in insects

can be predicted without a knowledge of the hormone structure. European Journal of Entomology 101: 29–35.

Socha R., Šula J., Kodrík D., 2004. Wing morph-related differences in developmental pattern in accessory gland

proteins in adult males of Pyrrhocoris apterus (L.) and their endocrine control. Journal of Insect Physiology 50: 893–901.

Vybiral S., Pešanová Z., Barczayova L., Janský L., 2004. Pyrogenic effects of cytokines (IL-1 IL-6 TNF) and

their mode of action on thermoregulatory centers and functions. Journal of Thermal Biology 30: 19–28.

2005 Eizaguirre M., Schafellner C., Lo´pez C., Sehnal F., 2005. Relationship between an increase of juvenile hormone

titer in early instars and the induction of diapause in fully grown larvae of Sesamia nonagrioides. Journal of Insect Physiology 51: 1127–1134.

Hussein H.M., Dimetry N., Zidan Z., Iss-Hak R.R., Sehnal F., 2005. Effects of insect growth regulators on the

hairy rose beetle, Tropinota squalida (Col., Scarabeidae). Journal of Applied Entomology 129: 142–148.

Hussein H.M., Habuštová O., Sehnal F., 2005. Beetle-specific Bacillus thuringiensis Cry3Aa toxin reduces

larval growth and curbs reproduction in Spodoptera littoralis (Boisd.). Pest Management Science 61: 1186–1192.

Kludkiewicz B., Kodrík D., Grzelak K., Nirmala X., Sehnal F., 2005. Structurally unique recombinant Kazal-

type proteinase inhibitor retains activity when terminally extended and glycosylated. Protein Expression and Purification 43: 94–102.

Kodrík D., Socha R., 2005. The effect of insecticide on adipokinetic hormone titre in the insect body. Pest

Management Science 61: 1077–1082. Neuwirth A., Kodrík D., Birkenbeil H., Sehnal F., 2005. Tris stimulates ecdysteroid secretion via

Ca2+messenger system in the prothoracic glands of Locusta migratoria. Physiological Entomology 30: 270–277.

Socha R., Kodrík D., Šula J., 2005. Wing morph-specific differences in the metabolism and endocrine control of

reserve mobilization in adult males of a flightless bug, Pyrrhocoris apterus (L.) (Heteroptera). Journal of Comparative Physiology B-Biochemical Systematic and Environmental Physiology 175: 557–565.

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Department of Botany

Staff Prof. RNDr. Karel Prach, CSc. (Head) – [email protected] – plant ecology, restoration

ecology

Prof. Dr. Sándor Bartha – (external member, Inst. Ecology and Botany, Hungarian Academy

of Sciences, Vácrátót) – [email protected] – plant ecology

Prof. RNDr. Jiří Komárek, DrSc. – [email protected] – algology

Prof. RNDr. Jan Lepš, CSc. – [email protected] – plant ecology, biostatistics

Doc. RNDr. Josef Elster, CSc. (external member) – [email protected] – ecology of algae

Doc. RNDr. Lubomír Hrouda, CSc. (external member) – [email protected] – taxonomy

and phytogeography of higher plants

Doc. RNDr. Jitka Klimešová, CSc. (external member) – [email protected] – plant

ecology

Mgr. Marek Bastl – [email protected] – plant ecology, biostatistics

PhDr. Jaromír Beneš – [email protected] – archeobotany

RNDr. Jan Kaštovský, Ph.D. – [email protected] – algology

Ing. Miloslava Kavková, Ph.D. – [email protected] – mycology

RNDr. Zdenka Křenová, Ph.D. – [email protected] – plant ecology (population ecology)

Mgr. Jan Kučera, Ph.D. – [email protected] – taxonomy of bryophytes

RNDr. Jaromír Lukavský, CSc. (external member) – [email protected] – algology

RNDr. Stanislav Mihulka, Ph.D. – [email protected] – plant ecology (ecology of invasive

plants)

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RNDr. Majka Šmilauerová, Ph.D. – [email protected] – plant ecology (root systems)

RNDr. Miroslav Šrůtek, CSc. – [email protected] – forest ecology

Ing. Milan Štech, Ph.D. – [email protected] – taxonomy and phytogeography of higher

plants

Scientific activities In the past two years, the Department of Botany conducted research activities predominantly

in the following fields, mostly as continuations of previous studies: taxonomy and ecology of

cyanobacteria and algae, taxonomy of bryophytes, ecology of selected groups of fungi,

taxonomy and phytogeography of higher plants, ecology of populations and communities of

higher plants, vegetation ecology, restoration ecology, and archeobotany. Particular topics are

evident in the titles of the publications listed below, however the following ones may be

highlighted: Taxonomic studies on cyanobacteria are oriented mostly on polyphasic

evaluation of diversity of selected genera, mainly from planktic and extreme biotopes. The

traditional cytomorphological, ecological, and molecular approachs are applied for revision of

strains and natural populations. The cyanobacterial microflora of extreme biotopes is studied

mainly in maritime Antarctica, and in various tropical habitats in American continents.

In a comparative study carried out in five European countries it was demonstrated that

sampling effect is the most important part of the biodiversity effect in the diversity–

productivity studies. The conclusion was confirmed by a pot experiment conducted in

controlled conditions. The most successful woodland invasion in Papua New Guinea

confirmed the rule that in moist tropics, invasions are restricted disturbed habitats.

Comparisons of a higher number of successional seres in human disturbed habitats revealed a

pattern across the seres, distinguishing between “ruderal” and “non-ruderal” seres being more

important than primary or secondary status of succession. Substratum pH appeared to be the

main driving environmental variable. Proportions of woody species used in ancient

settlements were quantified using archeobotanical samples, and based on this, the forest

vegetation around the settlements was tentatively reconstructed.

Some research and education activities were conducted in close collaboration with the

Institute of Botany, Academy of Sciences of the Czech Republic, as well as with several

foreigner universities and institutes, e.g. University of Groningen, and University of

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Wageningen, The Netherlands; Institute of Botany and Ecology, Hungarian Academy of

Sciences; University of Liverpool, and University of Sheffield, England; University of

California, Davis; University of Uppsala; University of Helsinki; University of Palermo and

others. Two projects within the EU programme Framework V are carried out together with

two international consorcia, comprising altogether more than ten European countries.

The department organised various excursions inside the country and abroad, among them

to the Crimean Peninsula or to the Kola Peninsula. At the end of 2002, the whole Department

moved into a newly reconstructed building, which supported closer collaboration and

communication among the particular groups inside the Department, which had been located in

four distant sites.

Study programmes The Department of Botany co-ordinates study programmes and student theses in botany. This

department is responsible for the master program “Botany” with six specialisations:

“Algology” – guaranteed by J. Komárek, “Mycology” – guaranteed by M. Kavková,

“Systematics of Higher Plants” – guaranteed by M. Štech and J. Kučera, “Ecology of

Populations and Communities” – guaranteed by J. Lepš, “Vegetation Ecology” – guaranteed

by K. Prach, “Archeobotany” – guaranteed by J. Beneš.

After finishing M.S. degree, students can take part in Ph.D. studies in the fields of botany.

Generally, an emphasis is put, besides theoretical disciplines and experimental approaches, on

broad field experience. The ability to recognise plants and their communities in the field and

observe their ecological relations is considered a basis for other research.


2004 Bastlová D., Čížková H., Bastl M., Květ J., 2004. Growth of Lythrum salicaria and Phragmites australis plants

originating from a wide geographical area: response to nutrient and water supply. Global Ecology and Biogeography 13: 259–271.

Beneš J., 2004. Paleoecology of the LBK: Earliest agriculturist and landscape Bohemia, Czech Republic, In:

Lukeš A., Zvelebil M. (eds), LBK Dialogues. Studies in formation of the Linear Pottery Culture, BAR International Series 1304: 143–150.

Callaghan T.V., Bjorn L.O., Chernov Y., Chapin T., Christensen T.R., Huntley B., Ims R.A., Johansson M.,

Jolly D., Jonasson S., Matveyeva N., Panikov N., Oechel W., Shaver G., Elster J., Henttonen H., Laine K., Taulavuori K., Taulavuori E., Zockler C., 2004. Biodiversity distributions and adaptations of arctic species in the context of environmental change. Ambio 33: 404–417.

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Callaghan T.V., Bjorn L.O., Chernov Y., Chapin T., Christensen T.R., Huntley B., Ims R.A., Johansson M., Jolly D., Jonasson S., Matveyeva N., Panikov N., Oechel W., Shaver G., Elster J., Jonsdottir I.S., Laine K., Taulavuori K., Taulavuori E., Zockler C., 2004. Responses to projected changes in climate and UV-B at the species level. Ambio 33: 418–435.

Ceschi-Berrini C., DeAppolonia F., Valle L.D., Komárek J., Andreoli C., 2004. Morphological and molecular

characterization of a thermophilic cyanobacterium (Oscillatoriales) from Euganean thermal springs (Padua, Italy). Archiv für Hydrobiologie/Algological Studies 113: 73–85.

Cronberg G., Komárek J., 2004. Some nostocalean cyanoprokaryotes from lentic habitats of Eastern and

Southern Africa. Nova Hedwigia 78: 71–106. Day J.G., Lukavský J., Lorenz M., Friedl T., Elster J., Campbell C.N., Brand J.J., 2004. Pringsheim’s living

legacy: CAUP CCALA CCAP SAG and UTEX culture collections of algae. Nova Hedwigia 79: 27–37.

Doležal J., Ishii H., Vetrova V.P., Sumida A., Hara T., 2004. Tree growth and competition in a Betula

platyphylla-Larix cajanderi post-fire forest in central Kamchatka. Annals of Botany 94: 333–343. Doležal J., Šťastná P., Hara T., Šrůtek M., 2004. Neighbourhood interactions and environmental factors

influencing old-pasture succession in the Central Pyrenees. Journal of Vegetation Science 15: 101–108.

Elster J., Benson E.E., 2004. Life in the Polar Terrestrial Environment: A Focus on Algae. In: Benson E.E. et al.

(eds), Life in the frozen state. Taylor & Francis, London, pp. 111–149. Chaloupecká E., Lepš J., 2004. Equivalence of competitor effects and tradeoff between vegetative multiplication

and generative reproduction: case study with Lychnis flos-cuculi and Myosotis nemorosa. Flora 199: 157–167.

Janeček Š., Janečková P., Lepš J., 2004. Influence of soil heterogeneity and competition on growth features of

three meadow species. Flora 199: 3–11. Jonášová M., Prach K., 2004. Central-European mountain spruce (Picea abies (L.) Karst.) forests: regeneration

of tree species after a bark beetle outbreak. Ecological Engineering 23: 15–24. Klimešová J., Martínková J., Kočvarová M., 2004. Biological flora of Central Europe: Rorippa palustris (L.)

Besse. Flora 199: 453–463. Kočí V., Lukavský J., Mlejnek K., Kochánková L., Grabic R., Ocelka T., 2004. Application of semipermeable

membrane device (SPMD) for assessment of organic substances dangerous for green microalga Scenedesmus subspicatus. Archiv für Hydrobiologie/Algological Studies 111: 173–186.

Komárek J., Cepák V., Kaštovský J., Sulek J., 2004. What are the cyanobacterial genera Cyanothece and

Cyanobacterium? Contribution to the combined molecular and phenotype taxonomic evaluation of cyanobacterial diversity. Archiv für Hydrobiologie/Algological Studies 113: 1–36.

Lepš J., 2004. Variability in population and community biomass in a grassland community affected by

environmental productivity and diversity. Oikos 107: 64–71. Lepš J., 2004. What do the biodiversity experiments tell us about consequences of plant species loss in the real

world? Basic and Applied Ecology 5: 529–534. Martínková J., Klimešová J., Mihulka S., 2004. Resprouting after disturbance: an experimental study with short-

lived monocarpic herbs. Folia Geobotanica 39: 1–12. Mataloni G., Komárek J., 2004. Gloeocapsopsis aurea a new subaerophytic cyanobacterium from maritime

Antarctica. Polar Biology 27: 623–628.

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Novotný V., Miller S.E., Lepš J., Bito D., Janda M., Hulcr J., Basset Y., Damas K., Weiblen G.D., 2004. No tree an island: the plant-caterpillar food web of secondary rainforest in New Guinea. Ecology Letters 7: 1090–1110.

Petrů M., Menges E.S., 2004. Shifting sand in Florida scrub gaps and roadsides: Dynamic microsites for herbs.

American Midland Naturalist 151: 101–113. Prach K., 2004. Book review: The restoration and management of derelict land: Modern approach, Wong M.H.,

Bradshaw A.D. Restoration Ecology 12: 130. Suda J., Malcová R., Abazid D., Banas M., Procházka F., Šída O., Štech M., 2004. Cytotype distribution in

Empetrum (Ericaceae) at various spatial scales in the Czech Republic. Folia Geobotanica 39: 161–171.

Špačková I., Lepš J., 2004. Variability of seedling recruitment under dominant, moss and litter removal over four

years. Folia Geobotanica 29: 41–55. Via-Ordorika L., Fastner J., Kurmayer R., Hisbergues M., Dittmann E., Komárek J., Erhard M., Chorus I., 2004.

Distribution of microcystin-producing and non-microcystin-producing Microcystis sp. in European freshwater bodies: Detection of microcystins and microcystin genes in individual colonies. Systematic and Applied Microbiology 27: 592–602.

2005 Davis M.A., Pergl J., Truscott A., Kollmann J., Bakker J.P., Domenech R., Prach K., Prieur-Richard A.,

Veeneklaas R.M., Pyšek P., del Moral R., Hobbs R.J., Collins S.L., Pickett S.T.A., Reich P.B., 2005. Vegetation change: a reunifying concept in plant ecology. Perspectives in Plant Ecology, Evolution and Systematics 7: 69–76.

Day J., Benson E., Harding K., Knowles B., Idowu M., Bremmer D., Santos L., Santos F., Friedl T., Lorenz M.,

Lukešová A., Elster J., Lukavský J., Herdman M., Rippka R., Hall T., 2005. Cryopreservation and conservation of microalgae: The development of a pan-european scientific and biotechnological resource (The COBRA project). Cryo Letters 26: 231– 238.

de Bello F., Lepš J., Sebastia M., 2005. Predictive value of plant traits to grazing along a climatic gradient in the

Mediterranean. Journal of Applied Ecology 42: 824–833. Janeček Š., Lepš J., 2005. Effect of litter, leaf cover and cover of basal internodes of the dominant species

Molinia caerulea on seedling recruitment and established vegetation. Acta Oecologica 28: 141–147.

Kavková M., Čurn V., 2005. Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) as a potential

mycoparasite on Sphaerotheca fuliginea (Ascomycotina: Erysiphales). Mycopathologia 159: 53–63.

Köckinger H., Kučera J., Stebel A., 2005. Pohlia nutans subsp. schimperi (Müll.Hal.) Nyholm, a neglected

Nordic moss in Central Europe. Journal of Bryology 27: 351–355. Komárek J., Anagnostidis K., 2005. Cyanoprokaryota -2. Teil/ 2nd Part: Oscillatoriales. In: Süsswasserflora von

Mitteleuropa 19/2, Büdel B., Krienitz L., Gärtner G., Schagerl M. (eds), Elsevier/Spektrum, Heidelberg, pp. 759.

Kučera J., Holyoak D.T., 2005. Lectotypification of Bryum moravicum Podp. (Bryopsida: Bryaceae). Journal of

Bryology 27: 161–168. Lanta V., Lepš J., 2005. Effect of functional group richness and species richness in manipulated productivity–

diversity studies: a glasshouse pot experiment. Acta Oecologia 29: 85–96. Rajaniemi P., Hrouzek P., Kaštovská K., Williame R., Rantala A., Hoffmann L., Komárek J., Sivonen K., 2005.

Phylogenetic and morphological evaluation of the genera Anabaena, Aphanizomenon, Trichormus

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and Nostoc (Nostocales, Cyanobacteria). International Journal of Systematic and Ecological Microbiology 55: 11–26.

Štech M., Drábková L., 2005. Morphometric and RAPD study of the Melampyrum sylvaticum group in the

Sudeten, the Alps and Carpathians. Folia Geobotanica: Journal of Ecology and Systematics. 40: 177–193.

Williamson M., Pyšek P., Jarošík V., Prach K., 2005. On the rates and patterns of spread of alien plants in the

Czech Republic, Britain, and Ireland. Ecoscience 12: 424–433. Znachor P., Jezberová J., 2005. The occurrence of a bloom-forming green alga Pleodorina indica (Volvocales)

in the downstream reach of the River Malse (Czech Republic). Hydrobiologia 541: 221–228.

Department of Ecology and Hydrobiology

Staff Prof. Ing. Hana Šantrůčková, CSc. (Head) – [email protected] – microbial ecology, nutrient

cycling in soil

Prof. RNDr. Zdeněk Brandl, CSc. – [email protected] – ecology of zooplankton,

predatory feeding

Doc. Ing. Jiří Kopáček, CSc. – [email protected] – limnology, acidification of the

catchment

Prof. RNDr. Josef Rusek, DrSc. – [email protected] – soil micromorphology, taxonomy and

ecology of collembolans, proturans, diplurans, elaterids

Prof. RNDr. Karel Šimek, CSc. – [email protected] – water microbiology, bacteria-

protozoa interactions

Prof. Ing. Miloslav Šimek, CSc. – [email protected] – soil nitrogen transformations, trace

gas formation and emission

Doc. Ing. Josef Hejzlar, CSc. – [email protected] – limnology of reservoirs, eutrofication

Doc. RNDr. Vlastimil Křivan, CSc. – [email protected] – population ecology,

mathematical ecology

Doc. RNDr. Jan Kubečka, CSc. – [email protected] – fish ecology

Doc. RNDr. Petr Šmilauer, Ph.D. – [email protected] – ecological statistics

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RNDr. Jakub Borovec, Ph.D. – [email protected] – limnology of lakes and reservoirs,

sediments

Mgr. Miloslav Devetter (external member) – [email protected] – hydrobiology, zooplankton

Mgr. Jiří Dušek, PhD. – [email protected] – wetland ecology, constructed wetlands

Dr. Keith Edwards – [email protected] – ecosystem management, wetland ecology

RNDr. Jaroslava Komárková, CSc. – [email protected] – plankton primary production,

taxonomy od algae

RNDr. Jan Květ, CSc. – [email protected] – production ecology of wetlands, conservation

and managment

RNDr. Jiří Macháček, CSc. – [email protected] – zooplankton ecology

Ing. Tomáš Picek, Ph.D. – [email protected] – soil microbiology, constructed wetlands

RNDr. Ota Rauch, CSc. – [email protected] – pedology, soil chemistry, plant–soil

interactions

RNDr. Viera Straškrabová, DrSc. – [email protected] – water microbiology, bacteria-

phyto- and zooplankton interactions

Mgr. Eva Uhlířová, PhD. – [email protected] – soil microbiology

RNDr. Jaroslav Vrba, CSc. – [email protected] – water microbiology, extracellular enzyme

activity

PhDr. Jana Vrbová – [email protected] – secretary of the Department, communication skills

RNDr. Vojtěch Vyhnálek, CSc. (external member) – [email protected] – environmental impact

assesment

Scientific activities 1. Nutrient fluxes in the ecosystem and catchment

Attention is paid to the effect of eutrofication and atmospheric acidification on biological and

chemical processes in lakes, sediments and soil. Investigating lakes and sediments, a special

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emphasis is put on C, P and N - cycling and on the regulation mechanisms of plankton

composition and dynamics. Soil studies are focused on C, N and P cycling in soil and on

regulation of soil microbial metabolism by temperature, moisture, texture and nitrogen

availability. A special emphasis is put on underlying mechanisms and processes of trace gas

formation and emission from the soil.

2. The effect of eutrofication and management on wetlands

The research deals with the water balance and C and N cycling in the eutrophicated wetland

ecosystems and on the reaction of the wetland plants on high load of nutrients. The results

contribute to the knowledge of nutrient removal and retention in constructed wetlands

designed for wastewater treatment.

3. The investigation of fish communities in the valley reservoirs

The research includes fish stock assessment, studies of fish feeding, migration, and behaviour

and population dynamics. Special part of the research activities is the adaptation of scientific

echosounders for studies of various parameters of water environment.

4. Evolutionary and population ecology

The research is carried out by a group of theoretical ecologists. The group synthesises existing

empirical information on the role of individual behaviour and life-histories in shaping

community dynamics, identify the limitations of existing theory in dealing with issues of

scaling from individuals to communities and have begun developing new mathematical tools

that explicitly deal with scaling from individual-level processes to community dynamics.

Study programmes The Department of Ecology and Hydrobiology co-ordinates study programmes and student

theses in ecology. This department guarantees one bachelor, four master and two Ph.D.

programmes. Students doing bachelor degree can study the “Environmental care” programme

both for decision-makers and for analysts. M.Sc. students can choose from among the

programmes of “Applied Ecology”, “Ecology”, “Limnology” and “Soil Biology”. After

finishing M.Sc. degree, students can take part in Ph.D. studies in the fields of “Ecology”, and

“Hydrobiology”.

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2004 Bastlová D., Čížková H., Bastl M., Květ J., 2004. Growth of Lythrum salicaria and Phragmites australis plants


Cressman R., Křivan V., Garay J., 2004. Ideal free distributions, evolutionary games and population dynamics in

multiple species environments. The American Naturalist 164: 473–489. Edwards K., 2004. Book review: Primary succesion and ecosystem rehabilitation. Restoration Ecology 12: 464–

465. Frouzová J., Kubečka J., 2004. Changes of acoustic target strength during juvenile perch development. Fisheries

Research 66: 355–361. Hrouzek P., Lukešová A., Šimek M., 2004. Comparison of light and dark nitrogenase activity in selected soil

cyanobacteria. Folia Microbiologica 49: 435–440. Kopáček J., Brzáková M., Hejzlar J., Nedoma J., Porcal P., Vrba J., 2004. Nutrient cycling in a strongly acidified

mesotrophic lake. Limnology and Oceanography 49: 1202–1213. Kopáček J., Hardekopf D., Majer V., Pšenáková P., Stuchlík E., Veselý J., 2004. Response of alpine lakes and

soils to changes in acid deposition: the MAGIC model applied to the Tatra Mountain region Slovakia-Poland. Journal of Limnology 63: 143–156.

Kopáček J., Kaňa J., Šantrůčková H., Picek T., Stuchlík E., 2004. Chemical and biochemical characteristics of

alpine soils in the Tatra Mountains and their correlation with lake water quality. Water Air and Soil Pollution 153: 304–327.

Křivan V., Schmitz O.J., 2004. Trait and density mediated indirect interactions in simple food webs. Oikos 107:

239–250. Křivan V., Vrkoč I., 2004. Should "handled" prey be considered? Some consequences for functional response

predator-prey dynamics and optimal foraging theory. Journal of Theoretical Biology 227: 167–174.

Oravecz O., Elhottová D., Krištůfek V., Šustr V., Frouz J., Tříska J., Márialigeti K., 2004. Application of

ARDRA and PLFA analysis in characterizing the bacterial communities of the food gut and excrement of saprophagous larvae of Penthetria holosericea (Diptera: Bibionidae): a pilot study. Folia Microbiologica 49: 83–93.

Pichlová R., Weber A., Gosser B., 2004. Leptodora kindtii survival in the laboratory. Aquatic Ecology 38: 537–

546. Pokorný J., Květ J., 2004. Aquatic plants and lake ecosystems. In: The lake handbook. Vol. 1. Limnology and

limnetic ecology, O´Sullivan P.E. et Reynolds, C.S. (eds). Oxford Blackwell Publishing LTD, pp. 309–340.

Porcal P., Hejzlar J., Kopáček J., 2004. Seasonal and photochemical changes of DOM in an acidified forest lake

and its tributaries. Aquatic Sciences 66: 211–222. Rusek J., 2004. Collembola succession on deposits from a chemical factory. Pedobiologia 48: 519–525. Straškrábová V., Izmest’eva L.R., Maksimova E.A., Fietz S., Nedoma J., Borovec J., Kobanova G.I.,

Shchetinina E.V., Pislegina E.V., 2004. Primary production and microbial activity in the euphotic zone of Lake Baikal (Southern Basin) during late winter. Global and Planetary Change 46: 57–73.

32

Šantrůčková H., Picek T., Tykva R., Šimek M., Pavlů B., 2004. Short-term partitioning of C-14-[U]-glucose in the soil microbial pool under varied aeration status. Biology and Fertility of Soils 40: 386–392.

Šantrůčková H., Vrba J., Picek T., Kopáček J., 2004. Soil biochemical activity and phosphorus transformations

and losses from acidified forest soils. Soil Biology and Biochemistry 36: 1569–1576. Šimek K., Jezbera J., Horňák K., Vrba J., Seďa J., 2004. Role of diatom-attached choanoflagellates of the genus

Salpingoeca as pelagic bacterivores. Aquatic Microbial Ecology 36: 257–269. Šimek M., Elhottová D., Klimeš F., Hopkins D.W., 2004. Emission of N2O and CO2, denitrification

measurements and soil properties in red clover and reygrass stands. Soil Biology and Biochemistry 36: 9–21.

Tříska J., Kuncová G., Macková M., Nováková H., Paasivirta J., Lahtiperä M., Vrchotová N., 2004. Isolation

and identification of intermediates from biodegradation of low chlorinated biphenyls (Delor-103). Chemosphere 54: 725–733.

Uzunov J., Vadineanu A., Adamescu M., Beltram G., David S., Květ J., Ott I., Piorkowski H., Sedláková J.,

Ulevicius A., Watt A., Young J., 2004. Inland aquatic habitats. In: Young, J. et al (eds), Conflicts between human activities and the conservation of biodiversity in agricultural landscapes grasslands forests wetlands and uplands in the Acceding and Candidate Countries (ACC). Banchory Centre for Ecology and Hydrology Banchory, pp. 57–74.

Vašek M., Kubečka J., 2004. In situ diel patterns of zooplankton consumption by subadult/adult roach Rutilus

rutilus, bream Abramis brama, and bleak Alburnus alburnus. Folia Zoologica 53: 203–214. Vašek M., Kubečka J., Peterka J., Čech M., Draštík V., Hladík M., Prchalová M., Frouzová J., 2004.

Longitudinal and vertical spatial gradient in the distribution of fish within a canyon-shaped reservoir. International Review of Hydrobiology 89: 352–362.

Vrba J., Callieri C., Bittl T., Šimek K., Bertoni R., Filandr P., Hartman P., Hejzlar J., Macek M., 2004. Are

bacteria major producers of extracellular glycolytic enzymes in aquatic environments? International Review of Hydrobiology 89: 102–117.

Vrchotová N., Tříska J., Urban O., Pěknic L., 2004. Variability of catechin and 4-hydroxyacetophenone

distribution in Norway spruce needles in relation to their position age and growing conditions. Environmental Pollution 131: 55–59.

2005 Brandl Z., 2005. Freshwater copepods and rotifers: predators and their prey. Hydrobiologia 546: 475–489. Cao X., Štrojsová A., Znachor P., Zapomělová E., Liu G., Vrba J., Zhou Y., 2005. Detection of extracellular

phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China). European Journal of Phycology 40: 251–258.

Čech M., Kratochvíl M., Kubečka J., Draštík V., Matěna J., 2005. Diel vertical migrations of bathypelagic perch

fry. Journal of Fish Biology 66: 685–702. Draštík V., Kubečka J., 2005. Fish avoidance of acoustic survey boat in shallow waters. Fisheries Research 72:

219–228. Edwards K., 2005. Assembly rules and restoration ecology: bridging the gap between theory and practise.

Restoration Ecology 13: 580–581. Edwards K., Mills K., 2005. Aboveground and belowground productivity of Spartina alterniflora (smooth

cordgrass) in natural and created Louisiana salt marshes. Estuaries 28: 252–265. Edwards K., Travis S., Proffitt E., 2005. Genetic effects of a large-scale Spartina alterniflora (smooth cordgrass)

dieback and recovery in the northern Gulf of Mexico. Estuaries 28: 204–214.

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Frouzová J., Kubečka J., Balk H., Frouz J., 2005. Target strength of some European fish species and its

dependence on fish body parameters. Fisheries Research 75: 86–96. Horňák K., Mašín M., Jezbera J., Bettarel Y., Nedoma J., Sime-Ngando T., Šimek K., 2005. Effects of decreased

resource availability, protozoan grazing and viral impact on a structure of bacterioplankton assemblage in a canyon-shaped reservoir. FEMS Microbiology Ecology 52: 315–327.

Jezbera J., Horňák K., Šimek K., 2005. Food selection by bacterivorous protists: insight from the analysis of the

food vacuole content by means of fluorescence in situ hybridization. FEMS Microbiology Ecology 52: 351–363.

Kopáček J., Borovec J., Hejzlar J., Ulrich K.U., Northon S.A., Amirbahman A., 2005. Aluminum control of

phosphorus sorption by lake sediments. Environmental Science and Technology 39: 8784–8789. Kopáček J., Klementová Š., Norton S.A., 2005. Photochemical production of ionic and particulate aluminum and

iron in lakes. Environmental Science and Technology 39: 3656–3662. Kopáček J., Stuchlík E., Wright R.F., 2005. Long-term trends and spatial variability in nitrate leaching from

alpine catchment-lake ecosystems in the Tatra Mountains (Slovakia-Poland). Environmental Pollution 136: 89–101.

Kopáček J., Veselý J., 2005. Sulfur and nitrogen emissions in the Czech Republic and Slovakia from 1850 till

2000. Atmospheric Environment 39: 2179–2188. Krištůfek V., Elhottová D., Chroňáková A., Dostálková I., Picek T., Kalčík J., 2005. Growth strategy of

heterotrophic bacterial population along successional sequence on spoil of brown coal colliery substrate. Folia Microbiologica 50: 427–435.

Mankiewicz J., Komárková J., Izydorczyk K., Jurczak T., Tarczynska M., Zalewski M., 2005. Hepatotoxic

cyanobacterial blooms in lakes of northern Poland. Environmental Toxicology 20: 499–506. Picek T., Dušek J., Zemanová K., 2005. Nitrogen transformations and microbial activities in gravel bed

horizontal subsurface flow wetlands – aerobic and anaerobic incubation experiments. Natural and Constructed Wetlands: Nutrients, Metals and Management. In: Vymazal, J. (ed.). Leiden, Backhuys Publishers, pp. 199–206.

Proffitt C.E., Chiasson R.L., Owens A.B., Edwards K., Travis S.E., 2005. Spartina alterniflora genotype

influences facilitation and suppression of high marsh species colonizing an early successional salt marsh. Journal of Ecology 93: 404–416.

Rejmánková E., Komárková J., 2005. Response of cyanobacterial mats to nutrient and salinity changes. Aquatic

Botany 83: 87–107. Rejmánková E., Komárková J., Rejmánek M., 2005. Delta15N as an indicator of N2-fixation by cyanobacterial

mats in tropical marshes. Biochemistry 673: 353–368. Straškrabová V., Izmest'yeva L.R., Maksimova E.A., Fietz S., Nedoma J., Borovec J., Kobanova G.I.,

Shchetinina E.V., Pislegina E.V., 2005. Primary production and microbial activity in the euphotic zone of Lake Baikal (Southern Basin) during late winter. Global and Planetary Change 46: 57–73.

Šantrůčková H., Bird M.I., Elhottová D., Novák J., Picek T., Šimek M., Tykva R., 2005. Heterotrophic fixation

of CO2 in soil. Microbial Ecology 49: 218–225. Šantrůčková H., Kurbatova J.A., Shibistova O.B., Šmejkalová M., Uhlířová E., 2005. Short-term kinetics of soil

microbial respiration – A general parameter across scales? In: Binkley D. (ed.). Trees and Soil Interactions, Implications to Global Climate Change. Kluwer Acad Press, Dordrecht, pp. 229–246.

34

Šimek K., Horňák K., Jezbera J., Mašín M., Nedoma J., Gasol J.M., Schauer M., 2005. Influence of top-down and bottom-up manipulations on the R-BT065 subcluster of beta-proteobacteria, an abundant group in bacterioplankton of a freshwater reservoir. Applied and Environmental Microbiology 71: 2381–2390.

Šourková M., Frouz J., Fettweis U., Bens O., Hüttl R.F., Šantrůčková H., 2005. Soil development and properties

of microbial biomass succession in reclaimed post mining sites near Sokolov (Czech Republic) and near Cottbus (Germany). Geoderma 129: 73–80.

Šourková M., Frouz J., Šantrůčková H., 2005. Accumulation of carbon, nitrogen and phosphorus during soil

formation on alder spoil heaps after brown-coal mining, near Sokolov (Czech Republic). Geoderma 124: 203–214.

Štrojsová A., Vrba J., Nedoma J., Šimek K., 2005. Extracellular phosphatase activity of freshwater

phytoplankton exposed to different in situ phosphorus concentrations. Marine and Freshwater Research 56: 417–424.

Štrojsová M., Vrba J., 2005. Direct detection of digestive enzymes in planktonic rotifers using enzyme-labelled

fluorescence (ELF). Marine and Freshwater Research 56: 189–195. Uhlířová E., Elhottová D., Tříska J., Šantrůčková H., 2005. Physiology and microbial community structure in

soil at extreme water content. Folia Microbiologica 50: 161–166. Uhlířová E., Šimek M., Šantrůčková H., 2005. Microbial transformation of organic matter in soils of montane

grasslands under different management. Applied Soil Ecology 28: 225–235.

Department of General Biology

Staff Doc. RNDr. Jan Kopecký, CSc. (Head) – [email protected] – immunology

Prof. RNDr. Richard Petrásek, CSc. – [email protected] – human physiology

Prof. MUDr. Karel Smetana, DrSc. – [email protected] – blood cytology, nucleolus

Prof. MUDr. Václav Zamrazil, DrSc. – [email protected] – clinical endocrinology,

malfunction of thyroid gland, iodine

Doc. RNDr. Richard Hampl, DrSc. – [email protected] – steroid hormones (biochemistry

and analytic)

Doc. Ing. Ivo Šafařík, DrSc. – [email protected] – enzymology, biomagnetic research and

technology

Doc. MUDr. Milan Špála, CSc. – [email protected] – human pathophysiology

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Doc. MUDr. Miroslav Vondra, DrSc. – [email protected] – clinical endocrinology, diabetes

Doc. RNDr. František Weyda, CSc. – [email protected] – morphology and anatomy of

arthropods, electron microscopy, digital imaging

Mgr. Ján Štěrba – [email protected] – clinical biochemistry, toxicology

Ing. Radovan Bílek, CSc. – [email protected] – proteo-hormones, biofactors

RNDr. Evžen Buchar, CSc. – [email protected] – pharmacology

RNDr. Jarmila Čeřovská, CSc. – [email protected] – anthropometrical aspects of nutrition

MUDr. Marie Dryáková, CSc. – diagnostic and treatment of diabetes

MUDr. Radmila Janovská – pathology

MUDr. Josef Klíma, CSc. – [email protected] – pharmacology

RNDr. Jan Kovář, CSc. – [email protected] – metabolism of lipoproteins,

atherosclerosis

RNDr. Jan Kvíčala, CSc. – [email protected] – trace elements in organism

MUDr. Jana Přádná – [email protected] – histology

MUDr. Miroslav Verner – [email protected] – clinical biochemistry

MUDr. Ivan Vonke – [email protected] – clinical hematology

RNDr. Zdenka Vrajová – [email protected] – methods of clinical immunology

MUDr. Pavel Žampach – [email protected] – clinical immunology

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Scientific activities The research in the Department of General Biology focuses on several different topics

including many areas of biomedicine, imunology, enzymology, insect morphology, and

methods for particle and protein separation, and digital imaging. The Department is devoted

to the study of molecules, microorganisms and cells, for the diagnosis and management of

illness and for the investigation of the mechanisms and pathogenesis of disease. Areas of

biomedical research include hemathology, immunology, clinical biochemistry, clinical

endocrinology, pharmacology and other biomedical fields.

In the field of enzymology, magnetic carriers, adsorbents and substrates (e.g., cross-linked

erythrocytes and modified Saccharomyces cerevisiae cells) have been developed and prepared

for enzyme isolation and characterisation. Some proteolytic enzymes and low-molecular-

weight organic compounds (e.g., tricyclic drugs) and xenobiotics (e.g., dyes) have been

isolated using magnetic adsorbents. Magnetic solid-phase extraction has been applied for the

preconcentration of malachite green and crystal violet from large volume of water or urine,

and selected xenobiotics have been determined using magnetically modified enzymes.

In insect functional morphology the research has dealt with ultrastructural aspects of the

ontogeny of fat body and glands (tarsal and labial) of two termite species. Ultrastructure and

function of various organs in tsetse flies, mites, primitive arthropods etc. has been studied.

Other topics focused upon are digital imaging – application of new technologies and

equipment for professional use in entomology (and biology in general).

Immunological research is focused on the immunomodulatory compounds present in tick

saliva and their effect on the transmission of important human pathogens like Borrelia

burgdorferi and tick-borne encephalitis virus. Another part of immunological research is

devoted to the research of immunity against microsporidia, causative agents of opportunistic

infections in AIDS patients.

Study programmes The Department of General Biology co-ordinates one bachelor and one master study

programme and student theses in general biology and biomedicine.

1. B. Sc. programme – “Biomedical Laboratory Technique”

(Supervisor F. Weyda). Teaches skills necessary for practical work in biological and health

institutions including diagnostic methods based on the analysis of nucleic acids and proteins.

37

Graduates will find positions in laboratories and in the industry of laboratory supplies and

equipment.

2. M. Sc. programme – “Clinical Biology”

(Supervisor R. Petrásek). The aim of this programme is to provide students with knowledge of

the principles and theories underlying the various methods and their application within the

fields of clinical biology and biomedicine. The programme is to help students develop the

skill of combining theoretical and technical knowledge in order to solve problems when

developing methods, performing and interpreting elaborate diagnostic tests and improving

quality. The programme also provides an appropriate educational and professional

environment, preparing students for specialisation at postgraduate level. Graduates will find

positions in clinical laboratories and in biomedical research institutions.


2004 Morais J.P.M.G., Azevedo R.B., Silva L.P., Lacava Z.G.M., Bao S.N., Silva O., Pelegrini F., Gansau C., Buske

N., Šafařík I., Šafaříková M., Morais P.C., 2004. Magnetic resonance investigation of magnetic-labeled baker's yeast cells. Journal of Magnetism and Magnetic Materials 272–276: 2400–2401.

Pechová J., Kopecký J., Salát J., 2004. Effect of tick salivary gland extract on the cytokine production by mouse

epidermal cells. Folia Parasitologica 51: 367–372. Salát J., Sak B., Le T., Kopecký J., 2004. Susceptibility of IFN-gamma or IL-12 knock-out and SCID mice to

infection with two microsporidian species Encephalitozoon cuniculi and E. intestinalis. Folia Parasitologica 51: 275–282.

Šafařík I., Šafaříková M., 2004. Magnetic nano- and microparticles in biosciences and biotechnology. In:

Nedkov I., Tailhades P. (eds). Nanoscale Magnetic Oxides and Bio-World. Sofia Heron Press, pp. 93–122.

Šenigl F., Kopecký J., Grubhoffer L., 2004. Distribution of E and NS1 proteins of TBE virus in mammalian and

tick cells. Folia Microbiologica 49: 213–216. 2005 Salát J., Kopecký J., Janečková B., Ditrich O., 2005. Different routes of infection with Encephalitozoon

intestinalis affect the development of immune response in immunocompetent and immunodeficient mice. Acta Parasitologica 50: 180–188.

Severinová J., Salát J., Kročová Z., Řezníčková J., Demová H., Horká H., Kopecký J., 2005. Co-inoculation of

Borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice. Folia Microbiologica 50: 457–463.

Šafařík I., Šafaříková M., Weyda F., Mosiniewicz-Szablewska E., Slawska-Waniewska A., 2005. Ferrofluid-

modified plant-based materials as adsorbents for batch separation of selected biologically active compounds and xenobiotics. Journal of Magnetism and Magnetic Materials 293: 371–376.

38

Šafaříková M., Kibriková I., Ptáčková L., Hubka T., Komárek K., Šafařík I., 2005. Magnetic solid phase extraction of non-ionic surfactants from water. Journal of Magnetism and Magnetic Materials 293: 377–381.

Šafaříková M., Ptáčková L., Kibriková I., Šafařík I., 2005. Biosorption of water-soluble dyes on magnetically

modified Saccharomyces cerevisiae subsp. uvarum cells. Chemosphere 59: 831–835. Šobotník J., Weyda F., Hanus R., 2005. Ultrastructural study of tergal and posterior sternal glands in

Prorhinotermes simplex (Isoptera : Rhinotermitidae). European Journal of Entomology 102: 81–88.

Department of Genetics

Staff Doc. RNDr. Jindřich Bříza, CSc. (Head) – [email protected] – general and plant genetics

Prof. Ing. Otomar Linhart, DrSc. – [email protected] – fish genetics

Prof. RNDr. František Marec, CSc. – [email protected] – genetics and molecular

cytogenetics of insects

Prof. Ing. Jaroslav Petr, DrSc. – [email protected] – animal genetics

Doc. RNDr. Miroslav Šíp, DrSc. – [email protected] – biophysics of nucleic acids

Doc. Ing. Josef Špak, DrSc. – [email protected] – plant virology

Doc. RNDr. Michal Žurovec, CSc. – [email protected] – molecular and cell biology,

animal genetics

RNDr. Jiří Macas, PhD. – [email protected] – plant molecular cytogenetics

RNDr. Jaroslav Matoušek, CSc. – [email protected] – plant molecular genetics

Mgr. Daniela Pavingerová, CSc. – [email protected] – genetic transformation of plants

RNDr. Karel Petrzik, CSc. – [email protected] – plant molecular virology

39

RNDr. Slavomír Rakouský, CSc. – [email protected] – plant tissue cultures and genetics

Mgr. Ondřej Scheinost – [email protected] – human genetics and cytogenetics

RNDr. Josef Vlasák, CSc. – [email protected] – molecular biology and genetics

Doc. RNDr. Michal Žurovec, CSc. – [email protected] – molecular and cell biology,

genetics, growth factors in Drosophila

PaedDr. Martina Žurovcová, PhD. – [email protected] – population genetics

Scientific activities The Department of Genetics deals with plant molecular genetics, namely transgenosis and

genetics of plant viruses, with molecular cytogenetics of plants and insects and with

population genetics of insects. In plant transgenosis, research has been carried out on the

introduction of selected cloned genes and antisense constructs (GUS, GFP, chalcone synthase,

etc.) into the genomes of selected plant species (tobacco, Chrysanthemum, potato, tomato,

flax). In molecular genetics of plant viruses, developmental relationships of SVBV

(strawberry vein banding virus) and PMV (potato mop virus) were studied. In molecular

cytogenetics, one research group has examined the structure of repetitive DNA sequences in

the family Viciaceae. Another group has mapped the occurrence of TTAGG telomeric repeats

in chromosomes of insects and other arthropods and on the basis of new findings, suggested a

hypothesis on the origin of TTAGG repeats.

Study programmes The department runs two specialized study programmes and co-ordinates student theses in

genetics.

The master programme “Experimental Biology” – specialisation: “Genetics and Gene

Engineering” provides broad options for the study of plant transgenosis, the structure of the

plant genome, plant and insect molecular cytogenetics, the genetics of plant viruses and

viroids, plant – microbe interactions, manipulations, and human genetics.

The Ph.D. programme “Molecular and Cell Biology and Genetics”, run together with the

Department of Molecular Biology, offers possibilities for doctoral studies in the molecular

40

genetics and cytogenetics of plants and insects, genetically modified plants and genetics of

plant viruses and viroids.


2004 Emelianov I., Marec F., Mallet J., 2004. Genomic evidence for divergence with gene flow in host races of the

larch budmoth. Proceedings of the Royal Society of London Series B 271: 97–105. Frydrychová R., Grossman P., Trubač P., Vítková M., Marec F., 2004. Phylogenetic distribution of TTAGG

telomeric repeats in insects. Genome 47: 163–178. Gaudinová A., Špak J., Dobrev P., Malbeck J., Motyka V., Vaňková R., 2004. Changes in phytohormone

contents in radish exposed to virus infection. Acta Physiologiae Plantarum 26: 129. Klerks M.M., Lindner J.L., Vašková D., Špak J., Jelkmann W., Schoen C.D., 2004. Detection and tentative

grouping of strawberry crinkle isolates. European Journal of Plant Pathology 110: 45–52. Matoušek J., Orctová L., Steger G., Riesner D., 2004. Biolistic inoculation of plants with viroid nucleic acids.

Journal of Viroogical Methods 122: 153–164. Matoušek J., Orctová L., Steger G., Škopek J., Moors M., Dědič P., Riesner D., 2004. Analysis of thermal stress-

mediated PSTVd variation and biolistic inoculation of progeny of viroid "thermomutants" to tomato and Brassica species. Virology 323: 9–23.

Mediouni J., Fuková I., Frydrychová R., Dhouibi M.H., Marec F., 2004. Karyotype sex chromatin and sex

chromosome differentiation in the carob moth Ectomyelois ceratoniae (Lepidoptera: Pyralidae). Caryologia 57: 184–194.


Dostálová R., Seidenglanz M., Tejklová E., Griga M., 2004. Transgenic plant products and their introduction into the environment and to crop protection systems, a risk assessment. In: Nap, J.-P., Atanassov, A., Stiekema, W. J. (eds). Proc. NATO Advanced Res. Workshop Genomics for Biosafety in Plant Biotechnology, Bansko, Bulgaria, 15–19 October, 2004, NATO Science Series: Life and Behavioural Science 359. IOS Press, Amsterdam, The Netherlands, pp. 173–184.

Schubert J., Matoušek J., Mattern D., 2004. Pathogen-derived resistance in potato to Potato virus Y: aspects of

stability and biosafety under field conditions. Virus Research 100: 41–50. Šmahel M., Pokorná D., Macková J., Vlasák J. 2004. Enhancement of immunogenicity of HPV16 E7 oncogene

by fusion with E. coli beta-glucuronidase. Journal of Gene Medicine 6: 1092–1101. Špak J., Přibylová J., Špaková V., Kubelková D., 2004. The presence of phytoplasma in black currant infected

with the blackcurrant reversion disease. Journal of Phytopathology 152: 1–6. Tomimura K., Špak J., Katis N.K., Jenner C.E., Walsh J.A., Gibbs A.J., Ohshima K., 2004. Comparisons of the

genetic structure of populations of Turnip mosaic virus in west east Eurasia. Virology 330: 408–423.

Vašková D., Špak J., Klerks M.M., Jelkmann W., Thompson J.R., 2004. Real-time NASBA detection of

strawberry vein banding virus. European Journal of Plant Pathology 110: 213–221.

41

Field course in tropical habitats in Papua New

Guinea

Students take part in the botanical and zoological expedition “Altai 06”

Afrotropical subterranean rodent Fukomys

mechowi in laboratory breeding colony

42

Seminars shape essential part of the study

Students take part in international conferences

and summer schools

Pub relaxation is fundamental to student´s life too

43

2005 Bystřická D., Lenz O., Mráz I., Piherová L., Kmoch S., Šíp M., 2005. Oligonucleotide-based microarray: A new

improvement in microarray detection of plant viruses. Journal of Virological Methods 128: 176–182.

Doležal T., Doleželová E., Žurovec M., Bryant P.J., 2005. A Role for Adenosine Deaminase in Drosophila

Larval Development. Plos Biology 35: 381–389. Doleželová E., Žurovec M., Doležal T., Šimek P., Bryant P.J., 2005. The emerging role of adenosine deaminases

in insects. Insect Biochemistry and Molecular Biology 35: 381–389. Fuková I., Nguyen P., Marec F., 2005. Codling moth cytogenetics: karyotype, chromosomal location of rDNA,

and molecular differentiation of sex chromosomes. Genome 48: 1083–1092. Hraška M., Rakouský S., Kocábek T., 2005. Use of a simple semiquantitative method for an appraisal of green

fluorescent protein (GFP) gene expression in transgenic tobacco plants. Biologia Plantarum 49: 313–316.

Macas J., Koblížková A., Neumann P., 2005. Characterization of Stowaway MITEs in pea (Pisum sativum L.)

and identification of their potential master elements. Genome 48: 831–839. Marec F., Neven L.G., Robinson A.S., Vreysen M., Goldsmith M.R., Nagaraju J., Franz G., 2005. Development

of genetic sexing strains in lepidoptera: from traditional to transgenic approaches. J. of Economic Entomology 98: 248–259.

Matoušek J., Schubert J., Ptáček J., Kozlová P., Dědič P., 2005. Complete nucleotide sequence and molecular

probing of Potato virus S genome. Acta Virologica 49: 195–205. Matoušek J., Vrba L., Novák P., Patzak J., De Keukeleire J., Škopek J., Heyerick A., Roldan-Ruiz I., De

Keukeleire D., 2005. Cloning and molecular analysis of the regulatory factor HiMyb1 in hop (Humulus lupulus L.) and the potential of hop to produce bioactive prenylated flavonoids. J. of Agricultural and Food Chemistry 53: 4793–4798.

Navrátilová A., Neumann P., Macas J., 2005. Long-range organization of plant satellite repeats investigated

using strand-specific FISH. Cytogenetic and Genome Research 109: 58–62. Neumann P., Požárková D., Koblížková A., Macas J., 2005. PIGY, a new plant envelope-class LTR

retrotransposon. Molecular Geneticsand Genomics 273: 43–53. Petrzik K., Holá M., Špak J., 2005. Complete nucleotide sequence of radish mosaic virus RNA polymerase gene

and phylogenetic relationships in the genus Comovirus. Acta Virologica 49: 271–275. Tek A.L., Song J.Q., Macas J., Jiang J.M., 2005. Sobo, a recently amplified satellite repeat of potato, and its

implications for the origin of tandemly repeated sequences. Genetics 170: 1231–1238. Vítková M., Král J., Traut W., Zrzavý J., Marec F., 2005. The evolutionary origin of insect telomeric repeats,

(TTAGG) N. Chromosome Research 13: 145–156. Vrba L., Matoušek J., 2005. Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants.

Biologia Plantarum 49: 371–380. Yoshido A., Marec F., Sahara K., 2005. Resolution of sex chromosome constitution by genomic in situ

hybridization and fluorescence in situ hybridization with (TTAGG)(n) telomeric probe in some species of Lepidoptera. Chromosoma 114: 193–202.

Yukawa Y., Felis M., Englert M., Stojanov M., Matoušek J., Beier H., Sugiura M., 2005. Plant 7SL RNA genes

belong to type 4 of RNA polymerase III-dependent genes that are composed of mixed promoters. Plant Journal 43: 97–106.

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Department of Mathematics, Physics and Chemistry

Staff Prof. RNDr. Pavel Kindlmann, DrSc. (Head) – [email protected] – evolutionary ecology,

mathematical modelling

Doc. RNDr. Šárka Klementová, CSc. – [email protected] – photochemistry, chemistry of

water

Mgr. Kateřina Houdková – [email protected] – statistics, mathematical modelling,

predator-prey systems

Mgr. Petra Janečková – [email protected] – ecology of terrestrial orchids

RNDr. Jana Jersáková, Ph.D. – [email protected] – ecology of terrestrial orchids

Mgr. Adriana Rico – [email protected] – edge effects, vertebrates

Mgr. Iva Schödelbauerová – [email protected] – metapopulations, ecology of terrestrial

orchids

RNDr. Jan Šíma, Ph.D. – [email protected] – chemistry

Kateřina Wotavová – [email protected] – ecology of terrestrial orchids

Scientific activities Due to its heterogenity the Department consists of two small and relatively independent

sections, represented usually by one scientist and a group of his/her students, each with its

own research programme. Their work is widely cited with more than 200 citations in SCI

(excluding autocitations) in 2002. This number is likely to rise, as their average age is well

below 40 years.

Research in the section of theoretical ecology focuses on the modelling of the life histories

of insects (mainly aphids and their predators) and plants (especially terrestrial orchids and

strategies of their pollination). Differential equations and the theory of evolutionarily stable

strategies are used as the main tools. Research in the section of chemistry is concentrated in

investigating photochemical decay of pesticides in freshwater systems, photochemical

45

oxygenation of biopolymers (related to photodynamic tumour treatment) and in

environmental analytical chemistry.

Study programmes The department provides, above all, educational support in non biological courses. However,

the department is also responsible for undergraduate programme leading to a M.Sc. degree

“Ecology” – specialisation: “Conservation Biology”.

Selected publications of the department 2004 Dostálková I., Kindlmann P., 2004. Evolutionarily stable strategies for random processes. Theoretical Population

Biology 65: 205–210. Halkett F., Harrington R., Hulle M., Kindlmann P., Menu F., Rispe C., Plantegenest M., 2004. Dynamics of

production of sexual forms in aphids: theoretical and experimental evidence for adaptive “coin-flipping” plasticity. The American Naturalist 163: E112–E125.

Jersáková J., Kindlmann P., 2004. Reproductive success and gender variation in nectarless vs. rewarding orchids.

International Journal of Plant Sciences 165: 779–785. Kindlmann P., Arditi R., Dixon A.F.G., 2004. A simple aphid population model. In: Simon J.C., Dedryver C.A.,

Rispe C., Hulle M. (eds). Aphids in a new millenium. INRA Editions, INRA, Paris, pp. 325–330. Kindlmann P., Aviron S., Burel F., Ouin A., 2004. Can the assumption of a non-random search improve our

prediction of butterfly fluxes between resource patches? Ecological Entomology 29: 447–456. Kindlmann P., Dixon A.F.G., 2004. Past and future models of predator-prey population dynamics: the

importance of generation time ratio. In: Soares A.O., Ventura M.A., Garcia V., Hemptinne J.L., (eds). Ecology of Aphidophaga: Biology, Ecology and Behaviour of Aphidophagous Insects. ARQUIPÉLAGO – Life and Marine Sciences, Bulletin of the University of the Azores, Ponta Delgada, Azores, Portugal, pp. 65–71.

Kindlmann P., Stadler B., 2004. Temporal fluctuations in throughfall carbon concentrations in a spruce forest.

Ecological Modelling 176: 381–388. Šíma J., Dědina J., Rychlovský P., 2004. The efficiency of the electrochemical generation of volatile hydrides

studied by radiometry and atomic absorption spectrometry. Spectrochimica Acta Part B-atomic Spectroscopy 59: 125–133.

Wotavová K., Balounová Z., Kindlmann P., 2004. Factors influencing the occurrence and extinction of the

terrestrial orchid, Dactylorhiza majalis, in wet meadows. Biological Conservation 118: 271–279. 2005 Haraštová-Sobotková M., Jersáková J., Kindlmann P., Čurn V., 2005. Morphometric and genetic divergence

among populations of Neotinea ustulata (Orchidaceae) with different flowering phenologies. Folia Geobotanica 40: 385–405.

Houdková K., Kindlmann P., 2005. Optimal foraging in insect predators: consequences for population dynamics.

In: Yasuda, H. (ed.), Proceedings of International Symposium on Biological Control of Aphids

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and Coccids. Tsuruoka, Japan, September 25–29, 2005, Faculty of Agriculture, Yamagata University, pp. 197–204.

Kindlmann P., Aviron S., Burel F., 2005. When is landscape matrix important for determining animal fluxes

between resource patches? Ecological Complexity 2: 150–158. Kindlmann P., Houdková K., 2005. Intraguild predation models: how relevant are they for aphidophagous

insects? In: Yasuda, H. (ed.), Proceedings of International Symposium on Biological Control of Aphids and Coccids. Tsuruoka, Japan, September 25–29, 2005, Faculty of Agriculture, Yamagata University, pp. 172–176.

Kindlmann P., Jersáková J., 2005. Floral display, reproductive success, and conservation of terrestrial orchids.

Selbyana 26: 136–144. Kindlmann P., Yasuda H., Kajita Y., Dixon A.F.G., 2005. Field test of the effectiveness of ladybirds in

controlling aphids. In: Hoddle M.S. (ed.). International Symposium on Biological Control of Arthropods. Davos, Switzerland, September 12–16, 2005, USDA Forest Service, FHTET-2005-08, pp. 441–447.

Kopáček J., Klementová Š., Norton S.A., 2005. Photochemical production of ionic and particulate aluminum and

iron in lakes. Environmental Science and Technology 39: 3656–3662. Mráček Z., Bečvář S., Kindlmann P., Jersáková J., 2005. Habitat preference for entomopathogenic nematodes,

their insect hosts and new faunistic records for the Czech Republic. Biological Control 34: 27–37. Mráček Z., Kindlmann P., Webster J., 2005. Steinernema affine (Nematoda: Steinernematidae), a new record for

North America and its distribution relative to other entomopathogenic nematodes in British Columbia. Nematology 7: 495–501.

Department of Molecular Biology and Biochemistry

Staf

Doc. RNDr. Julius Lukeš, CSc. (Head) – [email protected] – molecular biology of parasitic

protozoa

Prof. RNDr. František Sehnal, CSc. – [email protected] – insect endocrinology,

developmental biology

Doc. RNDr. Marek Jindra, CSc. – [email protected] – molecular biology and genetics of

D. melanogaster, B. mori and C. elegans

Doc. RNDr. Ivo Šauman, Ph.D. – [email protected] – molecular and developmental

biology, insect physiology and behavior, circadian rhythms

Mgr. Tomáš Doležal, Ph.D. – [email protected] – developmental genetics,

D. melanogaster as a model to study human pathologies

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Masako Asahina–Jindrová Ph.D. – [email protected] – molecular genetics of nematodes

Ing. Miroslav Oborník, Ph.D. – [email protected] – molecular taxonomy and

phylogenetics

Mgr. Lukáš Trantírek, Ph.D. – [email protected] – NMR structure of DNA and proteins

Scientific activities

1. Steroid signaling in insects and nematodes

Nuclear receptors of the steroid signaling pathway in model lepidopteran insects have been

characterized at the molecular level. We have developed new approaches for targeted gene

silencing using the novel method of RNA interference, applied to the organism via germline

transformation. The goal is to understand the genetics underlying the steroid control of

molting and metamorphosis in non-drosophilid insects.

Molecular identification of the drosophila ecdysoneless (ecd) gene based on available ecd

mutants will provide a general insight into the steroid signaling pathway.

The conserved nuclear receptor Ftz-F1, a crucial factor in steroidogenesis in mammals and

molting and metamorphosis in insects, has been genetically implicated in molting and the

gonad differentiation of the nematode Caenorhabditis elegans. Mechanisms of the Ftz-F1

mode of action are being investigated in our current projects.

2. Functional genetics of a stress-related coactivator

Using reverse genetic methods of mutagenesis and RNA interference in the classical genetic

models Drosophilla melanogaster and Caenorhabditis elegans, we identify the functions and

genetic interactions of a novel type coactivator in aging and stress.

3. Genetic and molecular analyses of growth factors in Drosophila

We focus on two types of growth factors: (i) a family of Imaginal Disc Growth Factors

(IDGFs) that cooperate with insulin signaling to stimulate the proliferation, polariyation and

motility of imaginal disc cells, and (ii) we have identified a previously unknown family of

polypeptide growth factors, named ADGFs for their similarity to adenosine deaminases

(ADA). Our projects aim to identify the developmental and physiological roles of growth

factors using a combination of genetic, molecular and cell biological techniques.

4. Molecular characterization of silk

Protein microsequencing and the isolation of silk-specific cDNAs has led to the identification

of the components of waxmoth (Galleria mellonella) silk, most of which have counterparts in

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the silkmoth (Bombyx mori). A previously unknown protein, which we named seroin,

probably has an antibacterial function, and so do two other low-molecular silk proteins that

we have discovered. The goal is to understand the purpose of all the components of

lepidopteran silk.

5. Molecular basis of the insect circadian clock and diurnal rhythmicity

Circadian biological clocks are found in most living organisms and their fundamental

properties are highly conserved. Although the fruitfly (Drosophilla melanogaster) served as a

premier model for the molecular analysis of the circadian rhythms, we have demonstrated that

there are striking differences between the molecular regulations of the circadian timing

system even among holometabolous insects. The long-term goal of this project is to gain a

generally more valid understanding of the cellular and molecular mechanisms that underlie

circadian rhythmicity in insects.

6. Molecular parasitology

We are interested in the structure of mitochondrial (=kinetoplast) DNA in primitive flagellates

of the order Kinetoplastida. It has a unique structure being composed of a network of

mutually interlocked circular molecules. Moreover, it encodes genes whose transcripts

undergo extensive editing. We study the evolution of the kinetoplast DNA and the extent and

mechanism of RNA editing. Recently we have been emploing the process of RNA

interference in order to selectively silence the transcription of genes involved in editing and in

packaging of the kinetoplast DNA.

Study programmes The department co-ordinates one graduate study programme and student theses in molecular

and cell biology.

Ph.D. programme – “Molecular and Cell Biology and Genetics”, supervised by F. Sehnal.

This course offers 3-year projects to those outstanding students who wish to excell in

molecular biology and genetics as methods of addressing basic biological questions.The

Doctoral theses of graduates must be published in recognized international journals and the

students will spend a part of the program in a collaborating laboratory abroad.

Selected publications of the department 2004

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Avliyakulov N.K., Lukeš J., Ray D.S., 2004. Mitochondrial histone-like DNA-binding proteins are essential for normal cell growth and mitochondrial function in Crithidia fasciculata. Eukaryotic Cell 3: 518–526.

Foy B.D., Myles K.M., Pierro D.J., Sanchez-Vargas I., Uhlířová M., Jindra M., Beaty B.J., Olson K.E., 2004.

Development of a new Sindbis virus transducing system and its characterization in three Culicine mosquitoes and two Lepidopteran species. Insect Molecular Biology 13: 89–100.

Gažiová I., Bonnette P.C., Henrich V.C., Jindra M., 2004. Cell-autonomous roles of the ecdysoneless gene in

Drosophila development and oogenesis. Development 131: 2715–2725. Jindra M., Gažiová I., Uhlířová M., Okabe M., Hiromi Y., Hirose S., 2004. Coactivator MBF1 preserves the

redox-dependent AP-1 activity during oxidative stress in Drosophila. EMBO Journal 23: 3538–3547.

Johansson C., Finger L.D., Trantírek L., Mueller T.D., Kim S., Laird-Offringa I.A., Feigon J., 2004. Solution

structure of the complex formed by the two N-terminal RNA-binding domains of nucleolin and a pre-rRNA target. Journal of Molecular Biology 337: 799–816.

Podlipaev S.A., Votýpka J., Jirků M., Svobodová M., Lukeš J., 2004. Herpetomonas ztiplika n. sp.

(Kinetoplastida: Trypanosomatidae) – parasite of the blood-sucking biting midge Culicoides kibunensis Tokunaga 1937 (Diptera: Ceratopogonidae). Journal of Parasitology 90: 342–347.

Podlipaev S.A., Sturm N., Fiala I., Fernandes O., Westenberger S.J., Dollet M., Campbell D.A., Lukeš J., 2004.

Diversity of insect trypanosomatids assessed from the spliced leader RNA and 5S rRNA genes and intergenic regions. Journal of Eukaryotic Microbiology 51: 283–290.


Dostálová R., Seidenglanz M., Tejklová E., Griga M., 2004. Transgenic plant products and their introduction into the environment and to crop protection systems, a risk assessment. In: Nap, J.-P., Atanassov, A., Stiekema, W. J. (eds.). Proc. NATO Advanced Res. Workshop Genomics for Biosafety in Plant Biotechnology, Bansko, Bulgaria, 15–19 October, 2004, NATO Science Series: Life and Behavioural Science 359. IOS Press, Amsterdam, The Netherlands, pp. 173–184.

Votýpka J., Lukeš J., Oborník M., 2004. Phylogenetic relationship of Trypanosoma corvi and other Old World

avian trypanosomes. Acta Protozoologica 43: 225–231. Zeiner G., Foldynová S., Sturm N.R., Lukeš J., Campbell D.A., 2004. SmDl is required for spliced leader RNA

biogenesis. Eukaryotic Cell 3: 241–244. Zemanová E., Jirků M., Mauricio I.L., Miles M.A., Lukeš J., 2004. Genetic polymorphism within the

Leishmania donovani complex: correlation with geographical origin. American Journal of Tropical Medecine and Hygiene 70: 613–617.

2005 Doležal T., Doleželová E., Žurovec M., Bryant P.J., 2005. A Role for Adenosine Deaminase in Drosophila

Larval Development. Plos Biology 35: 381–389. Doležel D., Vaněčková H., Šauman I., Hodková M., 2005. Is period gene causally involved in the photoperiodic

regulation of reproductive diapause in the linden bug, Pyrrhocoris apterus? Journal of Insect Physiology 51: 655–659.

Doleželová E., Žurovec M., Doležal T., Šimek P., Bryant P.J., 2005. The emerging role of adenosine deaminases

in insects. Insect Biochemistry and Molecular Biology 35: 381–389. Foldynová-Trantírková S., Paris Z., Sturm N.R., Campbell D.A., Lukeš J., 2005. The Trypanosoma brucei La

protein is a candidate poly(U) shield that impacts spliced leader RNA maturation and tRNA intron removal. International Journal for Parasitology 35: 359–366.

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Horváth A., Horáková E., Dunajčíková P., Verner Z., Pravdová E., Šlapetová I., Cuninková L., Lukeš J., 2005. Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei. Molecular Microbiology 58: 116–130.

Kludkiewicz B., Kodrík D., Grzelak K., Nirmala X., Sehnal F., 2005. Structurally unique recombinant Kazal-

type proteinase inhibitor retains activity when terminally extended and glycosylated. Protein Expression and Purification 43: 94–102.

Lukeš J., Hashimi H., Zíková A., 2005. Unexplained complexity of the mitochondrial genome and transcriptome

in kinetoplastid flagellates. Current Genetics 48: 277–299. Lukeš J., Horák A., Scholz T., 2005. Helminth genome projects: all or nothing. Trends in Parasitology 21: 265–

266. Marande W., Lukeš J., Burger G., 2005. Unique Mitochondrial Genome Structure in Diplonemids, the Sister

Group of Kinetoplastids. Eukaryotic Cell 4: 1137–1146. Neuwirth A., Kodrík D., Birkenbeil H., Sehnal F., 2005. Tris stimulates ecdysteroid secretion via Ca2+messenger

system in the prothoracic glands of Locusta migratoria. Physiological Entomology 30: 270–277. Pellis-van Berkel W., Verheijen M.H.G., Cuppen E., Asahina M., de Rooij J., Jansen G., Plasterk R.H.A., Bos

J.L., Zwarkruis F.J.T., 2005. Requirement of the Caenorhabditis elegans RapGEF pxf-1 and rap-1 for Epithelial Integrity. Molecular Biology of the Cell 16: 106–116.

Sychrovský V., Muller N., Schneider B., Smrečki V., Špirko V., Šponer J., Trantírek L., 2005. Sugar Pucker

Modulates the Cross-Correlated Relaxation Rates across the Glycosidic Bond in DNA. Journal of the American Chemical Society 127: 14663–14667.

Šauman I., Briscoe A.D., Zhu, H., Shi D., Froy O., Stalleicken J., Yuan Q., Casselman A., Reppert S.M., 2005.

Connecting the Navigational Clock to Sun Compass Input in Monarch Butterfly Brain. Neuron 46: 457–467.

Šilhánková M., Jindra M., Asahina M., 2005. Nuclear receptor NHR-25 is required for cell-shape dynamics

during epidermal differentiation in Caenorhabditis elegans. Journal of Cell Science 118: 223–232. Vondrušková E., van den Burg J., Zíková A., Ernst N.L., Stuart K., Benne R., Lukeš J. 2005. RNA Interference

Analyses Suggest a Transcript-specific Regulatory Role for Mitochondrial RNA-binding Proteins MRP1 and MRP2 in RNA Editing and Other RNA Processing in Trypanosoma brucei. Journal of Biological Chemistry 280: 2429–2438.

Wijová M., Moravec F., Horák A., Modrý D., Lukeš J., 2005. Phylogenetic position of Dracunculus medinensis

and some related nematodes inferred from 18S rRNA. Parasitological Research 96: 133–135.

Department of Parasitology

Staff Doc. RNDr. Václav Hypša, CSc. (Head) – [email protected] – molecular phylogeny of

parasites, host-parasite co-evolution

51

Prof. MVDr. Iva Dyková, DrSc. – [email protected] – protozoan parasites of fish,

histopathology of protozoan infections

Prof. RNDr. Libor Grubhoffer, CSc. – [email protected] – biochemistry and molecular

biology of vectors and pathogens

Prof. RNDr. Tomáš Scholz, CSc. – [email protected] – parasitic helminths of fish

Prof. RNDr. Jiří Vávra, DrSc. – [email protected] – parasitic protozoa, electron microscopy

RNDr. Oleg Ditrich, CSc. – [email protected] – medical and veterinary protozoology

RNDr. Petr Kopáček, CSc. – [email protected] – arthropod immunity, biochemistry of

arthropod defence molecules

Scientific activities At the Department of Parasitology, the research is focused on structural, ecological,

biochemical, phylogenetic, and molecular aspects of parasite biology.

Ultrastructural characterization has performed for several new species of fish

microsporidia and myxozoa. The epidemiological risk of various cryptosporidian species was

assessed on the basis of genotypization of isolates from various animals. The results proved

that the genotypes are not strictly host-specific and that in natural conditions cryptosporidia

can be transmitted between host species. Monoclonal antibodies have been prepared against

important causative agents of human microsporidioses.

The RPL3 gene from Trypanoplasma borreli has been cloned, and the ATP-binding

activity has been localized to N-terminal region of the protein. It has been shown that an

important trypanocidal drug suramin strongly inhibits the binding of [γ35S] ATP to RPL3 at

subclinical levels (the research has been carried out in collaboration with S. Svensson,

University of Linköping).

Topological status, catenation, transcription of mitochondrial genes, and editing of the

transcripts have been analyzed in kinetoplast DNA minicircles and maxicircles in primitive

flagellates. Based on the results, a novel scenario for the evolution of kinetoplast DNA and

RNA editing has been proposed.

The effect of extract from tick salivary glands on phagocytosis and the killing of Borrelia

afzelii spirochetes was has been demonstrated. The extract also inhibited the killing of

52

B. afzelii spirochetes by macrophages and reduced the production of two major defense

molecules of phagocytes, superoxide and nitric oxide. The results indicate that in addition to

reduced phagocytosis, suppression of macrophage microbicidal mechanisms contributed to

the inhibitory effect of tick saliva on the killing of Borrelia spirochetes, thus facilitating the

transmission of this important pathogen.

Several components of tick recognition and defense system have been purified and

biochemically and molecularly characterized (α2 – macroglobulin, gut lysozyme, plasma

lectin). The lectin of tick-borne agent, Borrelia burgdorferi, has been cloned and expressed in

bacterial and baculoviral expression systems.

Based on molecular data, phylogenetic relationships and evolutionary patterns have been

explored within several parasitic groups, e.g., in Coccidia (including evolutionary important

species Goussia janae, Choleoeimeria spp. and Sarcocystis singaporensis), amphizoic

amaebae of the genera Paramoeba and Neoparamoeba, kintoplastids of suborder Bodonina,

the bloodsucking bugs of the subfamily Triatominae, and selected lineages of tapeworms

(e.g., order Pseudophyllidea, which includes medically important species such as

Diphyllobothrium latum and Spirometra, is an artificial assemblage of two unrelated clades,

“Diphyllobothriidae” and “Bothriocephalidae”; reproductive isolation of two veterinary

important cestodes, E. crassum from Atlantic salmon and brown trout and E. salvelini from

brook trout has beens revealed by genetic study).

Study programmes The Department of Parasitology is responsible for the master and doctoral programmes in

“Parasitology”. This department also co-ordinates student theses in parasitology.


2004 Armbrust E.V., Berges J.A., Bowler C., Green B.R., Martinez D., Putnam N.H., Zhou S.G., Allen A.E., Apt

K.E., Bechner M., Brzezinski M.A., Chaal B.K., Chiovitti A., Davis A.K., Demarest M.S., Detter J.C., Glavina T., Goodstein D., Hadi M.Z., Hellsten U., Hildebrand M., Jenkins B.D., Jurka J., Kapitonov V.V., Kroger N., Lau W.W.Y., Lane T.W., Larimer F.W., Lippmeier J.C., Lucas S., Medina M., Montsant A., Obornik M., Parker M.S., Palenik B., Pazour G.J., Richardson P.M., Rynearson T.A., Saito M.A., Schwartz D.C., Thamatrakoln K., Valentin K., Vardi A., Wilkerson F.P., Rokhsar D.S., 2004. The genome of the diatom Thalassiosira pseudonana: Ecology, evolution, and metabolism. Science 306: 79–86.

Avliyakulov N.K., Lukeš J., Ray D.S., 2004. Mitochondrial histone-like DNA-binding proteins are essential for

normal cell growth and mitochondrial function in Crithidia fasciculata. Eukaryotic Cell 3: 518–526.

53

Bondarenko S., Hromada M., 2004. Aploparaksis mackoi n. sp. (Cestoda: Hymenolepididae), a parasite of the

common snipe Gallinago gallinago (L.) from the Slovak Republic. Systematic Parasitology 58: 63–67.

Bruňanská M., Scholz T., Ibraheem M.H., 2004. Ultrastructural particularities of the spermatozoon of the

cestode Electrotaenia malopteruri (Fritsch 1886) (Proteocephalidae : Gangesiinae) a parasite of Malapterurus electricus (Siluriformes : Malapteruridae) from the river Nile Egypt. Parasitological Research 93: 114–120.

Bruňanská M., Scholz T., Ibraheem M.H., 2004. Ultrastructural characters of the spermatozoon of the cestode

Corallobothrium solidum Fritsch 1886 (Cestoda: Proteocephalidea) a parasite of the electric catfish Malapterurus electricus. Parasitological Research 94: 421–426.

Bruňanská M., Scholz T., Nebesářová J., 2004. Reinvestigation of spermiogenesis in the proteocephalidean

cestode Proteocephalus longicollis (Zeder 1800). Journal of Parasitology 90: 23–29. de Chambrier A., Scholz T., Ibraheem M.H., 2004. Redescription of Electrotaenia malopteruri (Fritsch 1886)

(Cestoda : Proteocephalidae) a parasite of Malapterurus electricus (Siluriformes: Malapteruridae) from Egypt. Systematic Parasitology 57: 97–109.

Dyková I., Lom J., 2004. Advances in the knowledge of amphizoic amoebae infecting fish. Folia Parasitologica

51: 81–97. Fiala I., Dyková I., 2004. The phylogeny of marine and freshwater species of the genus Chloromyxum

Mingazzini 1890 (Myxosporea : Bivalvulida) based on small subunit ribosomal RNA gene sequences. Folia Parasitologica 51: 211–214.

Grubhoffer L., Kovář V., Rudenko, N., 2004. Tick lectins: structural and functional properties. Parasitology 129:

S1–S13. Gurney R.H., Nowak B.F., Dyková I., Kuris A.M., 2004. Histopathological effects of trypanorhynch

metacestodes in the digestive gland of a novel host Carcinus maenas (Decapoda). Diseases of Aquatic Organisms 58: 63–69.

Hajdušek O., Ditrich O., Šlapeta J., 2004. Molecular identification of Cryptosporidium spp. in animal and human

hosts from the Czech Republic. Veterinary Parasitology 122: 183–192. Jarkovský J., Koubková B., Scholz T., Prokeš M., Baruš V., 2004. Seasonal dynamics of Proteocephalus

sagittus in the stone loach Barbatula barbatula from the Haná River Czech Republic. Journal of Helminthology 78: 225–229.

Koubková B., Baruš V., Prokeš M., Dyková I., 2004. Raphidascaris acus (Bloch 1779) larvae infections of the

stone loach Barbatula barbatula (L.) from the River Haná Czech Republic. Journal of Fish Disease 27: 65–71.

Kuchta R., Scholz T., 2004. Bathycestus brayi n. gen. and n. sp. (Cestoda : Pseudophyllidea) from the deep-sea

fish Notacanthus bonaparte in the Northeastern Atlantic. Journal of Parasitology 90: 316–321. Kváč M., Ditrich O., Kouba M., Sak B., Vítovec J., Květoňová D. 2004. Failed attempt of Cryptosporidium

andersoni infection in lambs. Folia Parasitologica 51: 373–374. Logan F.J., Horák A., Štefka J., Aydogdu A., Scholz T., 2004. The phylogeny of diphyllobothriid tapeworms

(Cestoda: Pseudophyllidea) based on ITS-2 rDNA sequences. Parasitological Research 94: 10–15. Moravec F., Fiala I., Dyková I., 2004. Philometra thaiensis sp. nov. (Nematoda Philometridae) from Tetraodon

palembangensis and T. fluviatilis (Pisces) from fresh waters in Thailand with a key to Philometra spp. parasitic in the host's abdominal cavity. Acta Parasitologica 49: 319–324.

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Oros M., Hanzelová V., Scholz T., 2004. The cestode Atractolytocestus huronensis (Caryophyllidea) continues to spread in Europe: new data on the helminth parasite of the common carp. Diseases of Aquatic Organisms 62: 115–119.

Pechová J., Kopecký J., Salát J., 2004. Effect of tick salivary gland extract on the cytokine production by mouse

epidermal cells. Folia Parasitologica 51: 367–372. Podlipaev S.A., Sturm N., Fiala I., Fernandes O., Westenberger S.J., Dollet M., Campbell D.A., Lukeš J., 2004.

Diversity of insect trypanosomatids assessed from the spliced leader RNA and 5S rRNA genes and intergenic regions. Journal of Eukaryotic Microbiology 51: 283–290.

Podlipaev S., Votýpka J., Jirků M., Svobodová M., Lukeš J., 2004. Herpetomonas ztiplika n. sp. (Kinetoplastida:

Trypanosomatidae) – parasite of the blood-sucking biting midge Culicoides kibunensis Tokunaga 1937 (Diptera: Ceratopogonidae). Journal of Parasitology 90: 342–347.

Rudenko N., Golovchenko M., Cihlářová V., Grubhoffer L., 2004. Tick-borne encephalitis virus-specific RT-

PCR – A rapid test for detection of the pathogen without viral RNA purification. Acta Virologica 48: 167–171.

Sak B., Saková K., Ditrich O., 2004. Effects of a novel anti-exospore monoclonal antibody on microsporidial

development in vitro. Parasitology Research 92: 74–80. Salát J., Sak B., Le T., Kopecký J., 2004. Susceptibility of IFN-gamma or IL-12 knock-out and SCID mice to

infection with two microsporidian species Encephalitozoon cuniculi and E. intestinalis. Folia Parasitologica 51: 275–282.

Salgado-Maldonado G., Moravec F., Cabanas-Carranza G., Aguilar-Aguilar R., Sanchez-Nava P., Baez-Vale R.,

Scholz T. 2004. Helminth parasites of the tropical gar Atractosteus tropicus Gill from Tabasco Mexico. Journal of Parasitology 90: 260–265.

Scholz T., Aguirre-Macedo M.L., Choudhury A., 2004. Auriculostoma astyanace N. gen. N. sp. (Digenea:

Allocreadiidae) from the banded astyanax Astyamax fasciatus (Characiformes: Characidae) from Nicaragua with a reevaluation of neotropical Crepidostomum spp. Journal of Parasitology 90: 1128–1132.

Scholz T., Bray A.R., Kuchta R., Řepová R., 2004. Larvae of gryporhynchid cestodes (Cyclophyllidea) from

fish: a review. Folia Parasitologica 51: 131–152. Scholz T., Marcogliese D.J., Bourque J.F., Škeříková A., Dodson J.J., 2004. Occurrence of Proteocephalus

tetrastomus (Rudolphi, 1810) (Cestoda: Proteocephalidea) in larval rainbow smelt (Osmerus mordax) in North America: Identification of a potential pathogen confirmed. Journal of Parasitology 90: 425–427.

Scholz T., Škeříková A., Shimazu, T., Grygier, M.J., 2004. A taxonomic study of species of

Bothriocephalus Rudolphi, 1808 (Cestoda: Pseudophyllidea) from eels in Japan: morphological and molecular evidence for the occurence of B. claviceps (Goeze, 1782) and confirmation of the validity of B. japonicus Yamaguti, 1934. Systematic Parasitology 57: 87–96.

Šenigl F., Kopecký J., Grubhoffer L., 2004. Distribution of E and NS1 proteins of TBE virus in mammalian and

tick cells. Folia Microbiologica 49: 213–216. Škeříková A., Hypša V., Scholz T., 2004. A paraphyly of the genus Bothriocephalus Rudolphi, 1808 (Cestoda:

Pseudophyllidea) inferred from internal transcribed spacer-2 and 18s ribosomal DNA sequences. Journal of Parasitology 90: 612–617.

Šnábel V., Hanzelová V., Scholz T., Gerdeaux D., Cabaret J., 2004. Allozyme analysis of genetic variation and

polymorphism in Eubothrium salvelini and E. crassum (Cestoda: Pseudophyllidea) from alpine lakes. Parasitological Research 93: 290–295.

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Votýpka J., Lukeš J., Oborník M., 2004. Phylogenetic relationship of Trypanosoma corvi and other Old World avian trypanosomes. Acta Protozoologica 43: 225–231.

Zeiner G., Foldynová S., Sturm N.R., Lukeš J., Campbell D.A., 2004. SmDl is required for spliced leader RNA

biogenesis. Eukaryotic Cell 3: 241–244. Zemanová E., Jirků M., Mauricio I.L., Miles M.A., Lukeš J., 2004. Genetic polymorphism within the

Leishmania donovani complex: correlation with geographical origin. American Journal of Tropical Medecine and Hygiene 70: 613–617.

Žďárská Z., Nebesářová J., 2004. Transmission electron microscopy of presuded sensory receptors in the

forebody papillae of Crepidostomum metoecus (Digenea: Allocreadiidae). Folia Parasitologica 51: 27–32.

Žďárská Z., Scholz T., Nebesářová J., 2004. Ultrastructure of the apical glandular region of the scolex of

Proteocephalus (Cestoda: Proteocephalidae). Folia Parasitologica 51: 333–338. 2005 Borovičková B., Hypša V., 2005. Ontogeny of tick hemocytes: a comparative analysis of Ixodes ricinus and

Ornithodoros moubata. Experimental and Applied Acarology 35: 317–333. Bruňanská M., Scholz T., Ibraheem M., 2005. Spermiogenesis in the cestode Corallobothrium solidum Fritsch,

1886 (Proteocephalidea : Corallobothriinae). Acta Zoologica 86: 55–61. de Chambrier A., Scholz T., 2005. Redescription of Houssayela sudobim (Woodland, 1935) (Cestoda:

Proteocephalidea), a parasite of Pseudoplatystoma fasciatum (Pisces: Siluriformes) from the River Amazon. Systematic Parasitology 62: 161–169.

Doležal P., Šmíd O., Rada P., Zubáková Z., Bursa D., Suták R., Nebesářová J., Lithgow T., Tachezy J., 2005.

Giardia mitosomes and trichomonad hydrogenosomes share a comnon mode of protein targeting. Proceedings of the National Academy of Science 102: 10924–10929.

Dyková I., Boháčová L., Fiala I., Macháčková B., Pecková H., Dvořáková H., 2005. Amoebae of the genera

Vannella Bovee, 1965 and Platyamoeba Page, 1969 isolated from fish and their phylogeny inferred from SSU rRNA gene and ITS sequences. European Journal of Protistology 41: 219–230.

Dyková I., Nowak B., Crosbie P., Fiala I., Pecková H., Adams M., Macháčková B., Dvořáková H., 2005.

Neoparamoeba branchiphila n. sp., and related species of the genus Neoparamoeba Page, 1987: morphological and molecular characterisation of selected strains. Journal of Fish Diseases 28: 49–64.

Dyková I., Pecková H., Fiala I., Dvořáková H., 2005. Filamoeba sinensis sp. n., a second species of the genus

Filamoeba Page, 1967, isolated from gills of Carassius gibelio (Bloch, 1782). Acta Protozoologica 44: 75–80.

Dyková I., Pindová Z., Fiala I., Dvořáková H., Macháčková B., 2005. Fish-isolated strains of Hartmannella

vermiformis Page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions. Folia Parasitologica 52: 295–303.

Dyková I., Veverková M., Fiala I., Dvořáková H., 2005. Protacanthamoeba bohemica sp. n., isolated from the

liver of Tench, Tinca tinca (Linnaeus, 1758). Acta Protozoologica 44: 369–376. Grubhoffer L., Golovchenko M., Vancová M., Zacharová-Slavíčková K., Rudenko N., Oliver J., 2005. Lyme

borreliosis: insights into tick- / host-borrelia relations. Folia Parasitologica 52: 279–294. Hanzelová V., Kuchta R., Scholz T., Shinn A., 2005. Morphometric analysis of four species of Eubothrium

(Cestoda: Pseudophyllidea) parasites of salmonid fish: An interspecific and intraspecific comparison. Parasitology International 54: 207–214.

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Hyliš M., Weiser J., Oborník M., Vávra J., 2005. DNA isolation from museum and type collection slides of microsporidia. Journal of Invertebrate Pathology 88: 257–260.

Hypša V., Škeříková A., Scholz T., 2005. Phylogeny, evolution and host-parasite relationships of the order

Proteocephalidea (Eucestoda) as revealed by combined analysis and secondary structure characters. Parasitology 130: 359–371.

Kuchta R., Hanzelová V., Shinn A., Poddubnaya L., Scholz T., 2005. Redescription of Eubothrium fragile

(Rudolphi, 1802) and E. rugosum (Batsch, 1786) (Cestoda: Pseudophyllidea), parasites of fish in the Holarctic Region. Folia Parasitologica 52: 251–260.

Ležalová R., Tkadlec E., Oborník M., Šimek J., Honza M. 2005. Should males come first? The relationship

between offspring hatching order and sex in the black-headed gull Larus ridibundus. Journal of Avian Biology 36: 478–483.

Lom J., Dyková I., 2005. Microsporidian xenomas in fish seen in wider perspective. Folia Parasitologica 52: 69–

81. Lukeš J., Horák A., Scholz T., 2005. Helminth genome projects: all or nothing. Trends in Parasitology 21: 265–

266. Modrý D., Jirků M., Šumbera R., 2005. Three new species of Eimeria (Apicomplexa: Eimeriidae) from the

silvery mole rat Heliophobius argenteocinereus, Peters, 1846 (Rodentia: Bathyergidae) from Malawi. Journal of Parasitology 91(5): 1200–1203.

Oborník M., Green B.R., 2005. Mosaic Origin of the Heme Biosynthesis Pathway in Photosynthetic Eukaryotes.

Molecular biology and Evolution 22: 2343–2353. Poddubnaya L., Mackiewicz J., Bruňanská M., Scholz T., 2005. Ultrastructural studies on the reproductive

system of progenetic Diplocotyle olrikii (Cestoda, Spathebothriidea): Ovarian tissue. Acta Parasitologica 50: 199–207.

Rego R., Hajdušek O., Kovář V., Kopáček P., Grubhoffer L., Hypša V., 2005. Molecular cloning and

comparative analysis of fibrinogen-related proteins from the soft tick Ornithodoros moubata and the hard tick Ixodes ricinus. Insect Biochemistry and Molecular Biology 35: 991–1004.

Rudenko N., Golovchenko M., Edwards M., Grubhoffer L., 2005. Differential expression of Ixodes ricinus tick

genes induced by blood feeding or Borrelia burgdorferi infection. Journal of Medical Entomology 42: 36–41.

Rudenko N., Golovchenko M., Němec J., Volkaert J., Mallátová N., Grubhoffer L., 2005. Improved method of

detection and molecular typing of Borrelia burgdorferi sensu lato in clinical samples by polymerase chain reaction without DNA purification. Folia Microbiologica 50: 31–39.

Rudolf I., Golovchenko M., Šikutová S., Rudenko N., Grubhoffer L., Hubálek Z., 2005. Babesia microti

(Piroplasmida: Babesiidae) in nymphal Ixodes ricinus (Acari: Ixodidae) in the Czech Republic. Folia Parasitologica 52: 274–276.

Sak B., Ditrich O., 2005. Humoral intestinal immunity against Encephalitozoon cunicali (Microsporidia)

infection in mice. Folia Parasitologica 52: 158–162. Salát J., Kopecký J., Janečková B., Ditrich O., 2005. Different routes of infection with Encephalitozoon

intestinalis affect the development of immune response in immunocompetent and immunodeficient mice. Acta Parasitologica 50: 180–188.

Poddubnaya L., Mackiewicz J., Bruňanská M., Scholz T., 2005. Ultrastructural studies on the reproductive

system of progenetic Diplocotyle olrikii (Cestoda, Spathebothriidea): Ovarian tissue. Acta Parasitologica 50: 199–207.

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Rego R., Hajdušek O., Kovář V., Kopáček P., Grubhoffer L., Hypša V., 2005. Molecular cloning and comparative analysis of fibrinogen-related proteins from the soft tick Ornithodoros moubata and the hard tick Ixodes ricinus. Insect Biochemistry and Molecular Biology 35: 991–1004.

Rudenko N., Golovchenko M., Edwards M., Grubhoffer L., 2005. Differential expression of Ixodes ricinus tick

genes induced by blood feeding or Borrelia burgdorferi infection. Journal of Medical Entomology 42: 36–41.

Rudenko N., Golovchenko M., Němec J., Volkaert J., Mallátová N., Grubhoffer L., 2005. Improved method of

detection and molecular typing of Borrelia burgdorferi sensu lato in clinical samples by polymerase chain reaction without DNA purification. Folia Microbiologica 50: 31–39.

Rudolf I., Golovchenko M., Šikutová S., Rudenko N., Grubhoffer L., Hubálek Z., 2005. Babesia microti

(Piroplasmida: Babesiidae) in nymphal Ixodes ricinus (Acari: Ixodidae) in the Czech Republic. Folia Parasitologica 52: 274–276.

Sak B., Ditrich O., 2005. Humoral intestinal immunity against Encephalitozoon cunicali (Microsporidia)

infection in mice. Folia Parasitologica 52: 158–162. Vancová M., Nebesářová J., Grubhoffer L., 2005. Lectin-Binding characteristics a Lyme borreliosis spirochete

Borrelia burgdorferi sensu stricto. Folia Microbiologica 50: 229–238. Vávra J., Hyliš M., Oborník M., Vossbrinck C.R., 2005. Microsporidia in aquatic microcrustacea: the copepod

microsporidium Marssoniella elegans Lemmermann, 1900 revisited. Folia Parasitologica 52: 163–172.

Ždárská Z., Nebesářová J., 2005. Transmission electron microscopy of the scolex and neck microtriches of

Silurotaenia siluri (Batsch, 1786) (Cestode: Proteocephalidea). Parasitological Research 97: 98–102.

Wijová M., Moravec F., Horák A., Modrý D., Lukeš J., 2005. Phylogenetic position of Dracunculus medinensis

and some related nematodes inferred from 18S rRNA. Parasitological Research 96: 133–135.

Department of Plant Physiology and Anatomy

Staff Doc. Ing. Jiří Šantrůček, CSc. (Head) – [email protected] – plant physiology,

photosynthesis, plant water relations

Doc. RNDr. Ondřej Prášil, Ph.D. – [email protected] – adaptation of cyanobacteria and algae

to variable external conditions

Mgr. Dáša Bastlová, Ph.D. – [email protected] – plant ecophysiology

RNDr. Jiří Květoň, CSc. – [email protected] – biochemistry of photosynthesis

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Ing. Jana Nebesářová, CSc. – [email protected] – ultrastructural and surface research in

biology, electron microscopy

RNDr. Karel Roháček, CSc. – [email protected] – photosystem II, chlorophyll

fluorescence, photoacustic spectroscopy

RNDr. Jiří Šebestián, CSc. – [email protected] – microbiology, ethylen production, membrane

transport

RNDr. Ivan Šetlík, CSc. – [email protected] – plant physiology, biochem. and biophys. of

photosynthesis, cell biol. of autotrophic microorganisms

Doc. RNDr. František Vácha, Ph.D. – [email protected] – photosystem II reaction center

complexes, single molecule spectroscopy

Scientific activities 1. Photochemistry and biochemistry of photosynthesis

Photosynthetic processes in algae are studied with the emphasis on Photosystem II (PSII). The

research involves studies of the structure and dynamics of PSII proteins, the role of PSII in

the molecular ecology of photosynthetic organisms and the development of new methods and

instruments for photosynthesis research. PSII particles isolated from cyanobacterium

Synechococcus elongatus have been characterized using variable fluorescence and

thermoluminescence data, “best” detergents with minimal distortion of the acceptor side have

been found and crystals of the PSII reaction center obtained and tested for their X-ray

diffraction (Šetlíková, Šetlík et al.).

The mechanism of interaction of heavy metals (Cu, Zn) with photosynthetic apparatus of

algae has been studied. The experiments confirmed the role of the LHCII complex as a target

of heavy metal substitution of Mg2+ in chlorophylls (Šetlík, Prášil et al.). The novel

instrumentation recently developed allows measurements of variable fluorescence on a single

algal cell and to study processes of energy trapping in PSII in submiliseconds intervals (Šetlík

et al.). Photoadaptation and the effect of strong CO2 deficit have been studied using

fluorescence quenching measurements. A novel mechanism of fluorescence quenching has

been described which does not depend on either reorganization of chlorophyll proteins or the

accumulation of reduced pheophytin in reaction centers (Šiffel). Five chlorophyll a reaction

centers have been isolated and characterised using hole-burning spectroscopy and single

molecule spectroscopy (Vácha).

2. Leaf-atmosphere interactions

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The research aims to evaluate the efficiency of photosynthetic carbon fixation in terms of

water use, adaptations to dry atmosphere and elevated CO2 expressed in the stomatal function

or in the structure of cuticle. The role of stomata, leaf cuticle and mesophyll in CO2/H2O

exchange between chloroplasts/epidermis and atmosphere are studied. The research includes

biochemistry and functional aspects of epicuticular waxes as a transport limiting barrier,

spatial and time-based heterogeneity of stomatal conductance (stomatal patchiness), the effect

of abscisic acid (ABA) on photosynthetic carbon fixation and stable isotopes (13C and 18O)

fractionation in photosynthesis.

A novel approach has been developed allowing separation of the gas phase diffusion

(pores) from the solid phase (formed by polymer matrix and cuticular waxes) diffusion of

water molecules. We have concluded from our results that the solid cuticle on stomatous leaf

side is characterised by significantly lower barrier properties. It is argued that this might be a

consequence of a pronounced lateral heterogeneity of stomatous cuticles (Šantrůček,

Nebesářová et al., Šantrůček, Šimáňová et al.). Heterogeneity of stomatal conductance has

been measured analysing gas exchange signal and using chlorophyll fluorescence imaging.

We conclude that stomatal patchiness and the leaf carboxylation capacity vary in proportion

during ABA induced stress periods. Consequences in terms of the mesophyll effect on

stomata function are investigated (Šantrůček, Hronková et al.).

3. Anatomy and histochemistry of plant stem tissues

The study of hystogenesis of the sclerenchymatous ring in Pelargonium peltatum revealed

that the ring is a part of the central cylinder and belongs to the pericycle. It has been

determined that safranin shows an affinity to lignin and may be used for selective staining

(Beneš et al.).

4. Physiology of invasive behavior of wetland plant species

A correlation was found between growth and flowering characteristics of both Lythrum

salicaria and Phragmites australis and the population's position along the north-south transect

across Europe (Bastlová, Květ et al.).

Study programmes The department coordinates the master study programmes “Experimental Biology” –

specialisation: “Plant Physiology” (superviser J. Šantrůček), “Biophysics” (superviser

K. Roháček) and the Ph.D. programme “Plant Physiology and Development” (superviser

J. Šantrůček). The Ph.D. course offers advanced education in the fields of plant physiology

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and anatomy. It is supported by the experience of supervisors, scientists working in the

Institutes of the Academy of Sciences of the Czech Republic and employing new biochemical

and biophysical methods. This department also offers coordination of student theses in plant

physiology and anatomy.

Selected publications of the department 2004 Bastlová D., Čížková H., Bastl M., Květ J., 2004. Growth of Lythrum salicaria and Phragmites australis plants


Bína D., Vácha F., 2004. Absorbance changes accompanying the fluorescence induction in whole cells of purple

bacteria. Acta Physiologiae Plantarum 46: 174. Bruňanská M., Scholz T., Nebesářová J., 2004. Reinvestigation of spermiogenesis in the proteocephalidean

cestode Proteocephalus longicollis (Zeder 1800). Journal of Parasitology 90: 23–29. Bumba L., Havelková-Doušová H., Hušák M., Vácha F., 2004. Structural characterization of photosystem II

complex from red alga Porphyridium cruentum retainsic subunits of the oxygen-evolving complex. European Journal of Biochemistry 271: 2967–2975.

Bumba L., Hušák M., Vácha F., 2004. Interaction of photosystem 2-LHC2 supercomplexes in adjacent layers of

stacked chloroplast thylakoid membranes. Photosynthetica 42: 193–199. Küpper H., Ferimazová N., Šetlík I., Berman-Frank I., 2004. Traffic lights in Trichodesmium. Regulation of

photosynthesis for nitrogen fixation studied by chlorophyll fluorescence kinetic microscopy. Plant physiology 135: 2120–2133.

Küpper H., Šetlík I., Hlásek M., 2004. A versatile chamber for simultaneous measurements of oxygen exchange

and fluorescence in filamentous and thallous algae as well as higher plants. Photosynthetica 42: 579–584.

Kutá Smatanová I., Vácha F., Gavira J.A., Carcia-Ruiz J.M., 2004. Crystallization of phototystem II core

complexes from Pisum sativum. Acta Crystallografica 60: 131. Kutý M., Pšenčík J., Vácha F., 2004. Charge separation on photosystem II reaction centres. Acta Crystallografica

60: 178. Litvín R., Vácha F., 2004. Room temperature oxidation of the peripheral chloropkyll of photosystem II reaction

centre. Acta Physiologiae Plantarum 26: 98. Šantrůček J., Šimaňová E., Karbulková J., Šimková M., Schreiber L., 2004. A new technigue for measurement

of water permeability of stomatous cuticular membranes isolated from Hedera helix leaves. Journal of Experimental Botany 55: 1411–1422.

Žďárská Z., Nebesářová J., 2004. Transmission electron microscopy of presuded sensory receptors in the

forebody papillae of Crepidostomum metoecus (Digenea: Allocreadiidae). Folia Parasitologica 51: 27–32.

Žďárská Z., Scholz T., Nebesářová J., 2004. Ultrastructure of the apical glandular region of the scolex of

Proteocephalus (Cestoda: Proteocephalidae). Folia Parasitologica 51: 333–338. Župčanová A., Vácha F., Kutá-Smatanová I., 2004. Photosystem II reaction centres: isolation, reconstitution, and

crystallisation. Acta Crystallografica 60: 208.

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2005 Bumba L., Prášil O., Vácha F., 2005. Antenna ring around trimeric Photosystem I in chlorophyll b containing

cyanobacterium Prochlorothrix hollandica. Biochimica et Biophysica Acta – Bioenergetics 1708: 1–5.

Bumba L., Tichý M., Dobáková M., Komenda J., Vácha F., 2005. Localization of the PsbH subumit in

photosystem II from the Synechocytis 6803 using the His-tagged Ni-NTA Nanogold labeling. Journal of Structural Biology 152: 28–35.

Doležal P., Šmíd O., Rada P., Zubáková Z., Bursa D., Suták R., Nebesářová J., Lithgow T., Tachezy J., 2005.

Giardia mitosomes and trichomonad hydrogenosomes share a comnon mode of protein targeting. Proceedings of the National Academy of Science 102: 10924–10929.

Květoň J., Durchan M., Roháček K., Šantrůček J., Šesták Z., Vácha F., 2005. Pavel Šiffel (1954–2003) or life

full of chlorophyll. Photosynthetica 43: 323–328. Palenčár P., Kutý M., Vácha F., 2005. Force field development on pigments of photosystem 2 reaction centre.

Photosynthetica 43: 417–420. Schreiber L., Elshatshat S., Koch K., Lin J., Šantrůček J., 2005. AgCl precipitates in isolated cuticular

membranes reduce rates of cuticular transpiration. Planta 223: 283–290. Šiffel P., Šantrůček, J. 2005. Diurnal course of photochemical activity of winter-adapted Scots pine at subzero

temperatures. Photosynthetica 43: 395–402. Vácha F., Bumba L., Kaftan D., Vácha M., 2005. Microscopy and single molecule detection in photosynthesis.

Micron 36: 483–502. Vácha F., Pšenčík J., Kutý M., Šiffel P., Durchan M., 2005. Evidence for localisation of accumulated

chlorophyll cation on the D1-accessory chlorophyll in the reaction centre of Photosystem II. Photosynthesis Research 84: 297–302.

Valenta J., Janda P., Dohnalová K., Nižňanský D., Linnros J., Vácha F., 2005. Colloidal suspensions of silicon

nanocrystals from single nanocrystals to photonic structures. Optical Materials 27: 1046–1049. Ždárská Z., Nebesářová J., 2005. Transmission electron microscopy of the scolex and neck microtriches of

Silurotaenia siluri (Batsch, 1786) (Cestode: Proteocephalidea). Parasitological Research 97: 98–102.

Department of Zoology

Staff Doc. RNDr. Oldřich Nedvěd, CSc. (Head) – [email protected] – insect ecophysiology,

biology of ladybird beetles

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Prof. RNDr. Vojtěch Novotný, CSc. – [email protected] – insect ecology and

biodiversity, tropical ecology

Prof. RNDr. Tomáš Soldán, DrSc. – [email protected] – ecology and systematics of water

insects

Prof. RNDr. Zdeněk Veselovský, DrSc. – [email protected] – vertebrate ethology

Prof. RNDr. Jan Zrzavý, CSc. – [email protected] – animal phylogeny, arthropod

morphology

Doc. RNDr. Josef Matěna, CSc. – [email protected] – fish ecology, ecology of benthic

invertebrates

Doc. RNDr. František Sedláček, CSc. – [email protected] – mammal ecology, ethology,

and ecophysiology

Mgr. Michal Berec, Ph.D. – [email protected] – biology of amphibians and reptiles

RNDr. Roman Fuchs, CSc. – [email protected] – ecology and ethology of birds

Mgr. Jan Riegert, Ph.D. – [email protected] – biology of birds of prey

Mgr. Oldřich Říčan, Ph.D. – [email protected] – evolution of fish

Mgr. Radim Šumbera, Ph.D. – [email protected] – mammaliology

Scientific activities The Department of Zoology studies various aspects of the life of multicellular animals,

namely vertebrates and insects. Diverse methodical approaches are employed including field

observations of ecological and behavioural traits of native species in the Czech Republic, as

well as in other temperate and tropical areas; laboratory ethological and physiological

experiments, population genetics, morphology, taxonomy – most of the using phylogenetic

reconstruction.

Several years of studies in the lowland tropical rainforest in Papua New Guinea has

revealed broader host plant specificity than had been previously suggested: most herbivorous

insect species feed on several related plant species instead of on a single plant species each.

The huge species richness of insects in the tropics is still determined mostly by the plant

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species richness. Generalist insect predators like ground beetles facultatively scavenge on

decaying small animals. The DNA of the prey species can be detected for a few days in the

guts of predators using PCR The only species of the animal phylum Cycliophora described

till 2005, Symbion pandora, was known only from the Northern Sea on the host lobster

Nephrops norwegicus. Recently we have found a population in the Adriatic (Mediterranean)

Sea, on the lobster Homarus gammarus. Phylogenic relationships of recent dogs, wolves, and

foxes – Canidae (Mammalia, Carnivora) has been estimated employing both morphological

and molecular data.

Study programmes The Department of Zoology co-ordinates three study programmes and student theses in

zoology.

1. M. Sc. programme – “Zoology”

(Supervisors R. Fuchs, O. Nedvěd, J. Zrzavý). The department provides studies in three

specialisations – “Zoology of Vertebrates”, “Entomology” and “Animal Ecology”. These

studies are widely orientated to the aims and methods of study of current animal biology, with

emphasis on ecology and ethology of birds, mammals, and fish; and on insect morphology,

phylogeny, ecology, and ecophysiology. Graduates will be prepared to work in education,

nature conservation, and basic and applied research.

2. Ph. D. programme – “Zoology”

(Supervisor J. Zrzavý). This discipline is designed to provide students with a good review of

biology, ecology and the evolution of animals, and with a good knowledge of modern

zoological methodology. The graduates find work in basic and applied research institutes,

schools, environment institutions, zoos and museums. The Ph.D. studies are focused on

animal phylogeny, ecology and the behavior of fish, birds, and mammals, and vertebrate

population ecology.

3. Ph. D. programme – “Entomology”

(Supervisor O. Nedvěd). Study in this discipline includes both the basic and applied research

of various aspects of insect life. The current studies are specialized in arthropod ecology,

morphology, and ecophysiology, tropical ecology, and life history of biocontrol agents.


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2004 Basset Y., Novotný V., Miller S.E., Weiblen G.D., Missa O., 2004. Conservation and biological monitoring of

tropical forests: the role of parataxonomists. Journal of Applied Ecology 41: 163–174. Berec L., Boukal D.S., 2004. Implications of mate search, mate choice and divorce rate for population dynamics

of sexually reproducing species. Oikos 104: 122–132. Bezděk A., 2004. Catalogue of Diplotaxini (Coleoptera: Scarabaeidae: Melolonthinae) of the Old World.

Zootaxa No. 463. Magnolia Press, Auckland, New Zealand, 90 pp. Bezděk A., 2004. Revision of the genus Ceratogonia (Scarabaeidae: Melolonthinae: Diplotaxini). Annales

Zoologici 54: 797–801. Bezděk A., Cressey H.B., 2004. Cresseyella nom. nov., a new replacement name for the genus Apogonia

(Crustacea: Copepoda, Caligidae). Biologia 59: 180. Doležal P., Bextine B.R., Doležalová R., Miller T.A., 2004. Novel methods of monitoring the feeding behavior

of Homalodisca coagulata. Annals of the Entomological Society of America 97: 1055–1062. Fric Z., Konvička M., Zrzavý J., 2004. Red & black or black & white? Phylogeny of the Araschnia butterflies

(Lepidoptera: Nymphalidae) and evolution of seasonal polyphenism. Journal of Evolutionary Biology 17: 265–278.

Godunko R.J., Klonovska-Olejnik M., Soldán T., 2004. Ecdyonurus rizuni sp. nov. (Ephemeroptera:

Heptageniidae) from the Eastern Carpathians. Annales Zoologici 53: 519–524. Godunko R.J., Prokopov G.A., Soldán T., 2004. Mayflies of the Crimean Peninsula. III. The description of

Baetis milani sp. n. with notes to taxonomy of the subgenus Rhodobaetis Jacob, 2003 (Ephemeroptera: Baetidae). Acta Zoologica Cracoviensia 47: 231–248.

Hula V., Konvička M., Pavlíčko A., Fric Z., 2004. Marsh Fritillary (Euphydryas aurinia) in the Czech Republic:

Monitoring metapopulation structure and conservation of the endangered butterfly. Entomologica Fennica 15: 231–241.

Janda M., Folková D., Zrzavý J., 2004. Phylogeny of Lasius ants based on mitochondrial DNA and morphology

and the evolution of social parasitism in the Lasiini (Hymenoptera: Formicidae). Molecular Phylogenetics and Evolution 33: 595–614.

Kaňuščák P., Hromada M., Sparks T.H., Tryjanowski P., 2004. Does climate at different scales influence the

phenology and phenotype of the River Warbler Locustella fluviatilis? Oecologia 141: 158–163. Kumstátová T., Brinke T., Tomková S., Fuchs R., Petrusek A., 2004. Habitat preferences of tree pipit (Anthus

trivialis) and meadow pipit (A. pratensis) at sympatric and allopatric localities. Journal of Avian Biology 145: 334–342.

Lhota S., Junek T., Bartoš L., 2004. Alternative reproduction tactics in male aye-ayes: individual differences in

social behaviour and ranging patterns. Folia Primatologica 75: 88. Logan F.J., Horák A., Štefka J., Aydogdu A., Scholz T., 2004. The phylogeny of diphyllobothriid tapeworms

(Cestoda: Pseudophyllidea) based on ITS-2 rDNA sequences. Parasitological Research 94: 10–15. Nedvěd O., 2004. Occurence of the phylum cycliophora in the Mediterranean. Marine Ecology, Progress Series

277: 297–299. Novotný V., Basset Y., Miller S.E., Kitching R., Laidlaw M., Drozd P., Čížek L., 2004. How many insect

species feed on woody plants from one hectare of a lowland rainforest? Conservation Biology 18: 227–237.

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Novotný V., Miller S.E., Lepš J., Bito D., Janda M., Hulcr J., Basset Y., Damas K., Weiblen G.D., 2004. No tree an island: the plant-caterpillar food web of secondary rainforest in New Guinea. Ecology Letters 7: 1090–1110.

Pech P., Fric Z., Konvička M., Zrzavý J., 2004. Phylogeny of Maculinea blues (Lepidoptera:Lycaenidae) based

on morphological and ecological characters: evolution of parasitic myrmecophily. Cladistics 20: 362–375.

Renault D., Nedvěd O., Hervant F., Vernon P., 2004. The importance of fluctuating thermal regimes for

repairing chill injuries in the tropical beetle Alphitobius diaperinus (Coleoptera: Tenebrionidae) during low temperature exposures. Physiological Entomology 29: 139–145.

Riegert J., Fuchs R., 2004. Insect in the diet of urban kestrels from central Europe: An alternative prey or

constant component of the diet? Ornis Fennica 81: 23–32. Sedláček O., Fuchs R., Exnerová A., 2004. Redstart Phoenicurus phoenicurus and black redstart P. ochruros in a

mosaic urban environment: neighbours or rivals? Journal of Avian Biology 35: 336–343. Šklíba J., Fuchs R., 2004. Corncrake Crex crex extended their home ranges by visiting the territories of

neighbouring males. Bird Study 51: 113–118. Šumbera R., Chitaukali W.N., Elichová M., Kubová J., Burda H., 2004. Microclimatic stability in burrows of an

Afrotropical solitary bathyergid rodent the silvery mole-rat (Heliophobius argenteocinereus). Journal of Zoology 263: 409–416.

Vašek M., Kubečka J., 2004. In situ diel patterns of zooplankton consumption by subadult/adult roach Rutilus

rutilus, bream Abramis brama, and bleak Alburnus alburnus. Folia Zoologica 53: 203–214. Vašek M., Kubečka J., Peterka J., Čech M., Draštík V., Hladík M., Prchalová M., Frouzová J., 2004.

Longitudinal and vertical spatial gradient in the distribution of fish within a canyon-shaped reservoir. International Review of Hydrobiology 89: 352–362.

Zrzavý J., Řičánková V., 2004. Phylogeny of Recent Canidae (Mammalia, Carnivora): relative reliability and

utility of the morphological and molecular datasets. Zoologica Scripta 33: 311–333. 2005 Antczak M., Hromada M., Tryjanowski P., 2005. Frogs and toads in the food of the Great Grey Shrike (Lanius

excubitor): Larders and skinning as two ways to consume dangerous prey. Animal Biology 55: 227–233.

Antczak M., Hromada M., Tryjanowski P., 2005. Research activity induces change in nest position of the Great

Grey Shrike Lanius excubitor. Ornis Fennica 82: 20–25. Antczak M., Hromada M., Tryjanowski P., 2005. Spatio-temporal changes in Great Grey Shrike Lanius

excubitor impaling behaviour: From food caching to communication signs. Ardea 93: 101–107. Barčiová L., Macholán M., 2005. Morphometric study of two species of wood mice Apodemus sylvaticus and A.

flavicollis (Rodentia: Muridae): traditional and geometric morphometric approach. Acta Theriologica 51: 15–27.

Burda H., Šumbera R., Chitaukali W.N., Dryden G.L., 2005. Taxonomic status and remarks on ecology of the

malawian mole-rat Cryptomys whytei (Rodentia, Bathyergidae). Acta Theriologica 50: 529–536. Dooms C., Laurent P., Daloze D., Pasteels J., Nedvěd O., Braekman J.C., 2005. Synthesis and absolute

configuration of hyperaspine, an alkaloid of the ladybird Hyperaspis campestris. European Journal of Organic Chemistry 7: 1378–1383.

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Foltan P., Sheppard, S., Konvička M., Symondson W.O.C., 2005. The significance of facultative scavenging in generalist predator nutrition: detecting decayed prey in the guts of predators using PCR. Molecular Ecology 14: 4147–4158.

Hubálek Z., Burda H., Scharff A., Heth G., Nevo E., Šumbera R., Peško J., Zima J., 2005. Emmonsiosis of

subterranean (Bathyergidae, Spalacidae) in Africa and Israel. Medical Mycology 43: 691–697. Hulcr J., Pollet M., Ubik K., Vrkoč J., 2005. Exploitation of kairomones and synomones by Medetera spp.

(Diptera: Dolichopodidae), predators of spruce bark beetles. European Journal of Entomology 102: 655–662.

Kalushkov P., Nedvěd O., 2005. Genetically modified potatoes expressing Cry 3A protein do not affect

aphidophagous coccinellids. Journal of Applied Entomology 129: 401–406. Konopová B., Zrzavý J. 2005. Ultrastructure, development and homology of insect embryonic cuticles. Journal

of Morphology 264: 339–362. Lewinsohn T.M., Novotný V., Basset Y., 2005. Insects on plants: diversity of herbivore assemblages revisited.

Annual Reviews of Ecology, Evolution and Systematics 36: 597–620. Ležalová R., Tkadlec E., Oborník M., Šimek J., Honza M. 2005. Should males come first? The relationship

between offspring hatching order and sex in the black-headed gull Larus ridibundus. Journal of Avian Biology 36: 478–483.

Lukeš J., Horák A., Scholz T., 2005. Helminth genome projects: all or nothing. Trends in Parasitology 21: 265–

266. Modrý D., Jirků M., Šumbera R., 2005. Three new species of Eimeria (Apicomplexa: Eimeriidae) from the

silvery mole rat Heliophobius argenteocinereus, Peters, 1846 (Rodentia: Bathyergidae) from Malawi. Journal of Parasitology 91 (5): 1200–1203.

Novotný V., Basset Y., 2005. Host specificity of insect herbivores in tropical forests. Proceedings of the Royal

Society, London, Biological Sciences 272: 1083–1090. Novotný V., Clarke A.R., Drew R.A.I., Balagawi S., Clifford B., 2005. Host specialization and species richness

of fruit flies (Diptera: Tephritidae) in a New Guinea rain forest. Journal of Tropical Ecology 21: 67–77.

Novotný V., Miller S.E., Basset Y., Čížek L., Darrow K., Kaupa B., Kua J., Weiblen G.D., 2005. An altitudinal

comparison of caterpillar (Lepidoptera) assemblages on Ficus trees in Papua New Guinea. Journal of Biogeography 32: 1303–1314.

Novotný V., Weiblen G.D., 2005. From communities to continents: beta-diversity of herbivorous insects.

Annales Zoologici Fennici 42: 463–475. Půža V., Mráček Z., 2005. Seasonal dynamics of entomopathogenic nematodes of the genera Steinernema and

Heterorhabditis as a response to abiotic factors and abundance of insect hosts. Journal of Invertebrate Pathology 89: 116–122.

Tomášková L., Bejček V., Sedláček F., Šťastný K., Tkadlec E., Zima J., 2005. Population biology of shrews

(Sorex araneus and S. minutus) from a polluted area in central Europe. In: Merritt, J.F. et al. (eds): Advances in the Biology of Shrews II. Int. Soc. Shrew Biologists, 468 pp.

Veselovský Z., 2005. Etologie. Praha, Academia, Praha, 408 pp. Vítková M., Král J., Traut W., Zrzavý J., Marec F., 2005. The evolutionary origin of insect telomeric repeats,

(TTAGG) N. Chromosome Research 13: 145–156.

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Language Department

Staff Mgr. Petr Kos (Head) – [email protected] – English

Mgr. Monika Brandlová – [email protected] – English

Dr. Keith Edwards – [email protected] – Technical writing in English

Mgr. Petra Kaboňová – [email protected] – Spanish, English

Richard Klee, M.Sc. – [email protected] – English

Mgr. Linda Kocmichová – [email protected] – English

PhDr. Ivan Marek – [email protected] – English, German

Irina Mečířová – [email protected] – Russian

Mgr. Jana Šandová – [email protected] – English

Christopher Steer, B.A. – [email protected] – English

Mgr. Martina Vavreková – [email protected] – French

Mgr. David Žaloudek – [email protected] – English

The language department is not involved in scientific activities. It provides language

education and other services, such as translations and proof-reading, according to the needs of

the Faculty. It’s main focus is teaching both general and biology-based English, as all students

are required to take a final English examination. The department also provides Spanish,

French, German and Russian courses, as well as a course on writing scientific texts in

English.

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Graduation ceremony: second row – dean of the

Faculty (left), vice-rector, and vice-dean. First row – mace-bearer and secretary of the Faculty

Pretty and proud biologist

New graduates

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Location

Faculty of Biological Sciences, University of South Bohemia Branišovská 31, CZ-370 05 České Budějovice, Czech Republic

http://www.bf.jcu.cz/

Faculty of Biological Sciences - Jihoesk univerzita

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