Fabrication of an electrospun nanofibrous scaffold for use in the field of tissue engineering
Fabrication of an electrospun nanofibrous scaffold for use in the field of tissue engineering
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Transcript of Fabrication of an electrospun nanofibrous scaffold for use in the field of tissue engineering
Fabrication of an electrospun nanofibrous scaffold for use in the field
of tissue engineering
By: Shannon Daily & Tyler Crawford
Purpose To create a polycaprolactone mesh
which enables cell activity and seeks to eventually provide an application in the field of tissue engineering toward biomimetic skin graft.
Progress since last meeting Spun 8 more meshes
Visited Janelia Farm and used SEM
Details on cell procedure
Began cell work
Electrospun Meshes Mesh 5 & 6 Spun on 3/1 15 kV 15 cm from tip of pipette to collector
plate Pasteur pipette used Glass slide down middle
Electrospun Meshes
Mesh 7› 20 kV› 15 cm› No glass slide
Mesh 8› 20 kV› 20 cm› Glass slide
Mesh 7 & 8 Spun on 3/11 15 wt.% solution from 3/9
Electrospun Meshes
Mesh 9› 15 kV› 15 cm› Glass slide
Mesh 10› 20 kV› 15 cm› Glass slide
Mesh 9 & 10 Spun on 3/17 10 wt.% solution from 3/15
Electrospun Meshes
Mesh 11› 15 kV› 15 cm› Glass slide
Mesh 12› 20 kV› 15 cm› Glass slide
Mesh 11 & 12 Spun on 3/23 20 wt.% solution from 3/21
Chitosan Solution Created PCL/chitosan solution to begin
electrospinning 15 wt.% PCL, 1 wt.% chitosan
› 3 g PCL, .2 g chitosan, 16.8 g acetic acid
SEM ImagesMesh 4
SEM ImagesMesh 4
SEM ImagesMesh 6
SEM ImagesMesh 6
SEM ImagesMesh 6
Conclusion from SEM Beading is still a problem
› Sections of fibers and sections of beading Ideas to fix
› Increase the voltage› Decrease the concentration› Increase the distance
Comparison of fiber size› Mesh 1: 185 nm vs. Mesh 4&6: ~300 nm
Cell Work Meetings with Anu, Estelle, and Ms.
Curley› Determined basic procedure
Centrifuge thawed cells Add media to pellet of cells & resuspend Transfer to flask (in our case, tissue culture
plates) Allow cells to grow until about plate covered
about 80% Split to new containers
Cell Work Checked to see if mesh dissolves in
media› Placed small piece of mesh in 500 µL of
media› Let sit for 2 days- checking periodically› Mesh did not dissolve meaning cell growth
is possible
To be worked on: Use SEM on meshes created since last
time (7-12)› Different SEM?
Cell work› Begin with Mesh 4 (best fibers imaged)› Continue with other meshes
Begin spinning PCL/chitosan solutions
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