EQAS 2014 Salmonella spp.

Campylobacter spp.

April 24th, 2015

Mia Torpdahl (mtd@ssi.dk)Susanne Karlsmose Pedersen (suska@food.dtu.dk)

2

Objective

Presentation of obtained results for the EURL-AR and the ECDC FWD network for selected strains and antimicrobials of Salmonella and Campylobacter

Testing performed by: - The public health sector (ECDC FWD network)- The veterinary/food sector (EURL-AR network

Between the networks:- Differing methods applied for determining AMR profile- Differing criteria for categorization as S (I) R

3

Included strains and antimicrobialsSalmonella

4 strains: - S-9.1- S-9.2- S-9.7- S-9.8

4 antimicrobials: - Ampicillin (AMP)- Cefotaxime (FOT)- Ciprofloxacin (CIP) (pefloxacin

for disk)- Tetracycline (TET)

Campylobacter

4 strains: - C-9.1- C-9.2- C-9.3- C-9.7

Species ID + 3 antimicrobials: - Species identification (coli/jejuni)- Ciprofloxacin (CIP)- Erythromycin (ERY)- Tetracycline (TET)

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Method for performing AST and ID in the EURL-AR

Salmonella and Campylobacter ASTDemands set in EU/652/2013 (also listed in the protocol):- Dilution methods (broth microdilution or agar dilution)- List of antimicrobials (2 panels for Salmonella)- Range of dilutions- Interpretative criteria

Campylobacter species identification (C. coli, C. jejuni):

- Mandatory- In-house methods accepted- PCR-protocol suggested (available on the EURL-AR website)

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Method for performing AST and ID in the FWD-Net

Salmonella and Campylobacter ASTRecommended EU protocol for harmonised monitoring of antimicrobial resistance in human Salmonella and Campylobacter isolates:- Mandatory (4 Salmonella and 3 Campylobacter) and optional antimicrobials- Dilution methods (broth microdilution or agar dilution) and disk diffusion- Reporting of presently used methods including breakpoints/cutoffs- Reported values interpreted using EUCAST ECOFFs done by SSI for all

participants

Campylobacter species identification (C. coli, C. jejuni):

- Mandatory- In-house methods accepted

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EURL-AR Overall results (AST)

2/560

23/348

0

1

2

3

4

5

6

7

8

9

10

Salmonella Campylobacter

Dev

iatio

n le

vel(

%)

7

FWD-Net Overall results (AST)

8

EURL-AR Salmonella results – pr. lab

0

2

4

6

8

10

12

14

58 1 2 4 6 9 11 12 13 16 17 18 19 20 21 22 23 25 26 29 30 32 33 34 36 37 38 39 40 41 42 45 56 57 59

Dev

iatio

n le

vel(

%)

Deviation % Acceptance level

Laboratory number

Lab #58: 12.5% deviations based on 2 deviations out of 16 results

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FWD-Net Salmonella results – pr. lab – 31 labs

10

EURL-AR Salmonella results – antimicrobials

N=1

40

N=1

40

N=1

40

N=1

40

0

1

2

3

4

5

Dev

iatio

n le

vel (

%)

11

FWD-Net Salmonella results – antimicrobials

12

EURL-AR Salmonella results – strains

0

1

2

3

4

5D

evia

tion

leve

l (%

)

13

FWD-Net Salmonella results – strains

EURL-AR Campylobacter results – pr. lab

14

0

5

10

15

20

25

30

35

40

45

50

29 40 34 42 36 1 4 19 30 39 41 59 2 6 9 11 12 14 17 18 20 21 22 23 25 26 32 33 37 45 56 58

Dev

iatio

n le

vel (

%)

Deviation % Acceptance level

Laboratory number

4/9

4/12

2/12

3/12

1/12

Defined as outliers (full set of results)

FWD-Net Campylobacter results – pr. lab – 26 labs

15

16

EURL-AR Campylobacter results – antimicrobials

6/124

8/124 9/123

0123456789

10

Dev

iatio

n le

vel(

%)

17

FWD-Net Campylobacter results – antimicrobials

18

EURL-AR Campylobacter results – strains

2/96

9/92

6/93 6/90

0123456789

101112

Dev

iatio

n le

vel (

%)

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FWD-Net Campylobacter results – strains

FWD-NetIn total; 10 deviations in 104 tests (9,6% deviation)- C-9.1 (exp. C. jejuni) L001, - C-9.2 (exp. C. coli) L001, L015, L036- C-9.3 (exp. C. coli) L001, L015, L022. L025- C-9.7 (exp. C. jejuni) L001, L036

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Obtained results (species ID, Campylobacter)

EURL-ARIn total; 6 deviation in 128 tests (4.7% deviation)- C-9.1 (exp. C. jejuni) lab #36- C-9.2 (exp. C. coli) lab #40- C-9.7 (exp. C. jejuni) lab #19, #36, #40, #42

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Summing up

Challenges, Salmonella- Due to lack of tests in the whole range of antimicrobial dilutions, one

laboratory’s results were excluded from the analysis (EURL-AR)- Due to lack of tests in the whole range of antimicrobial dilutions,

several laboratory results were excluded from the analysis (FWD)

Challenges, Campylobacter- Two laboratories identified as outliers (EURL-AR)- One laboratories identified as outlier (FWD)- Due to lack of tests in the whole range of antimicrobial dilutions, one

laboratory’s results were excluded from the analysis (EURL-AR)- Species identification (EURL-AR and FWD)