Extraction of Nicotine
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Transcript of Extraction of Nicotine
Extraction of Nicotine By: DJ Hall and Brittany Hicks
PurposeDetermine if nicotine is extractable by means of
supercritical fluidRun more than one sample type to determine if
this makes a significant difference
SFESupercritical Fluid Extraction
Uses high pressure CO2
Used to extract organics from different types of matrixes
Allows for easy separation
ProblemsAll soluble organics will be separated form matrix
GC-MSGas Chromatography Mass Spectroscopy
Separation of violate organicsDetermine the analyte separated at specific
retention times using MS
Nicotine (162.12g/mol)Poison (parasympathomimetic alkaloid)Formed in nightshade family of plantsAntiherbivor chemicalSmall doses
StimulantRelaxation
Large dosesFatal
Extraction2 Cigarettes Marlboro blend 272 Pieces Nicorette Gum 2mg50 Drops of e-cigarette liquid 2.4% by weight
Extraction cont.All extractions were obtained by bubbling into
methanol and using SFE methanol modifier pumpCigarettes
Ground using mortar and pestle
Nicorette GumCut into small pieces
E-cigarette liquidCelite as inert matrix
Determination of NicotineAll samples were diluted to 50ml of methanolA standard was created by diluting 4mg of
Nicotine in 50ml of methanolStandards and Samples were run using the GC-MS
Reference Mass Spectra
Standard
Nicotine peak
Cigarettes
Nicotine peak
Cigarettes cont.
E-Cigarette Liquid
Nicotine peak
E-Cigarette Liquid Cont.
Nicorette Gum
Chromatogram ComparisonE-Cigarette Liquid
Nicorette Gum
Cigarette
Standard
ConclusionIt is possible to extract nicotine using the SFEBoth the E-Cigarette and Cigarette samples
contained nicotineThe Nicorette Gum showed undetectable amounts
of nicotineNicotine may have be contained in the gum residue
that was filtered before dilution
Future ProjectQuantify the amounts of nicotine extractedDetermine the extraction efficiency
Work CitedFischer, M. and Jefferies, T. Optimization of
Nicotine Extraction from Tobacco Using Supercritical Fluid Technology with Dynamic Extraction Modeling. J.Agric. Food Chem. (1996), 44, 1258-1264.