[ Care and Use ManUal ]

Extraction Manifold

Cont Ent s

I. IntroductIon

II. IdentIfyIng Parts

III. usIng the Waters extractIon ManIfold

IV. needle t IP InstallatIon Procedure

V. sPare Parts and accessorIes

I. IntroductIon

The Waters Extraction Manifold features:

■■ Positions for up to twenty extraction cartridges.

■■ Long needle tips which channel the cartridge effluents directly into the corresponding collection vessels and eliminate immediate fittings which could be a source of sample loss or cross-contamination in other manifold designs.

■■ Vacuum gauge, control and release valves for monitoring and adjustment of vacuum level to control flow rate.

■■ A choice of four racks to accommodate a variety of collection vessels.

■■ Three-point interlocking system to ensure proper orientation of racks, keying cartridge position to corresponding collection vessel.

■■ Chemically resistant, non-contaminating construction of glass, polyethylene, polypropylene, and Delrin™ components.

■■ Individually controlled, needle-tipped valves (stopcocks), available as accessories, permit simultaneous processing of samples when drying of traditional SPE cartridges must be avoided.

Figure 1. Waters 20-Position Extraction Manifold.

Luer Plug

Chamber

Needle TipBall Ring:for vacuum release

Vacuum Gauge

VacuumControl Valve

Cover: its three legs interlock with rack top plate

Rack (option for 13 x 100 mm tubes shown)

Exit Valve: connected to vacuum pump with inline liquid trap using 3/8” ID vacuum tubing.

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Extraction Manifold

II. IdentIfyIng Parts

Before assembling the Waters Extraction Manifold, ensure that you have these parts (refer to Figure 1 on previous page):

In Extraction Manifold Package:

■■ Glass chamber with exit valve installed

■■ White Delrin cover* with polyethylene gasket, vacuum gauge, control and release valves installed

■■ Needle tips, long (polypropylene), 20/pk

■■ Luer plugs, male (polypropylene), 25/pk

■■ Needle tip ejector tool (not shown)

Packaged separately:

■■ Rack, 20-position, as specified

*The three posts on the underside of the cover have two purposes. First, they serve as elevated feet so that the cover can be set down conveniently on the bench top without damaging the needle tips that protrude through the top of the cover. Second, they align only one way with the corresponding notches in the top plate on a rack. This makes is easier to keep track of a particular sample/cartridge and its corresponding collection vessel.

caution

■■ Glass vessels under vacuum have a danger of implosion if struck or damaged.

■■ Inspect the glass chamber for cracks, scratches, or other defects prior to each use.

■■ Do not use a chamber with visible defects.

■■ Do not exceed a vacuum level of 22” Hg (560 mm Hg).

III. usIng the Waters extractIon ManIfold

A typical solid-phase extraction (SPE) procedure consists of the following steps:

a. Condition/equilibrate the cartridge bed.

b. Load the sample.

c. Wash off interferences.

d. Elute analyte(s) of interest.

The Waters Extraction Manifold permits these steps to be carried out on up to twenty cartridges in parallel. Figure 1 shows the manifold assembled (without cartridges or valves) with a rack of tubes in place ready to collect the eluates in Step d. In Steps a–d, a rack is not necessary as the eluates from these steps normally

are treated as waste. The waste is drawn down into a liquid trap through the exit valve at the base of the glass chamber. However, when optimizing or troubleshooting an SPE protocol, it may be desirable to collect fractions in Steps b, c, and d; in this case, a separate set of tubes will be used for each of these steps.

Procedure

1. Connect the vacuum source to the outlet of a liquid trap using vacuum tubing. Connect the trap inlet to the exit valve at the case of the glass chamber using 3/8” I.D. vacuum tubing. Attention: Make certain that the liquid trap is compatible with, and

has sufficient capacity to collect, all the waste that will be generated

in your SPE procedure. If the trap capacity is too small, waste may

contaminate and/pr damage the vacuum pump.

2. Put the cover on the glass chamber.

3. Install a value (stopcock) in each of the 20 ports with the valve handle pointing outward, perpendicular to the edge of the chamber, so it is easily accessible for manipulation. Turn the body of each valve slightly to seal its needle tip firmly in its hold. The tips will extend into the mouths of the corresponding collection vessels, once a rack is in place underneath the cover.

4. Put an extraction cartridge into each valve hub and seat it firmly.

5. Close all valves by turning handles until each indicator shows that valve is in the closes position. See figure 2. When using Waters Oasis® HLB Cartridges, stopcocks are not needed.

Instead, install a needle tip with female Luer hub into each of the 20

ports. Make sure that the ridges on the outside of a needle tip (at the

top just under the hub) mate with the grooves inside the hold in the

cover. Seal all unused tips with make Luer plugs. Put an Oasis HLB

Cartridge into each unplugged needle tip hub. See Figure 3.

6. Turn on the vacuum pump. Adjust control valve to set the desired vacuum level.

7. Follow SPE protocol recommended for the type of cartridge used and the application being done. Add fluid into all cartridge reservoirs for each step before opening the valves to start or resume flow.

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Extraction Manifold

Oasis™ HLB Cartridge

Needle Tip (polypropylene)

Attention: Proper use of many SPE cartridges requires that the liquid level does not fall below the top frit. This is

especially true for Steps a and b listed above. Close each valve, as necessary, so as to prevent the cartridge from

drying out. Otherwise poor recovery and/or reproducibility may result. (See references on Waters website: www.

waters.com/oasis). This precaution is not necessary when using Waters Oasis HLB Extraction Cartridges.

Figure 2. Waters Sep-Pak® Cartridges and most SPE devices are firmly seated into the Luer hubs atop valves. (Sep-Pak Classic Cartridges require male-male Luer adapters for this purpose.) Valve (stopcock) handle is rotated to an open or closed position (as indicated) to control the flow through an individual cartridge.

Figure 3. Waters Oasis HLB Extraction Cartridges are fitted directly to the needle tips. Note: No valves (stopcocks) are needed.

8. To install or remove a rack of collection vessels, first close the exit valve (the pump can stay on; alternatively, turn off the pump). Break the vacuum in the manifold changer completely by slightly offsetting the white ball from its seal (see Figure 4). Then remove the cover. After a rack as been installed or removed, replace the cover with the corresponding notches on the top plate of the rack. Then, open the exit valve (and, if you had turned it off, turn on the vacuum pumps) to re-evacuate the chamber.Vacuum may be temporarily increased by putting your finger over the bleed port hold at the end of the vacuum

control valve (see Figure 4). Once fluid flow begins, release your finger to restore preset vacuum level.

Sep-Pak® Vac Cartridge

Valve (stopcock)OPEN CLOSED

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Extraction Manifold

Needle Tip

Luer Plugs: use to seal open

needle tips

White Rubber Ball: push it up or down slightly to

release vacuum in chamberLocking Nut: (in center) tighten it to secure set position of vacuum control valve

Vacuum Control Valve: rotate outer end to adjust vacuum to desired level

Bleed Port Hole: cover it with yourfinger tip to increase vacuum temporarily

To remove a cartridge from the manifold, turn it while pulling it up. The valve or needle tip) will remain in its socket. To remove a valve or needle tip from the cover, pry it out carefully with the ejector tool.

5. For installed needle tips that are not in use, install the Luer plug by pushing it down in the needle tip(s) input hold (see Figure 7). Luer plus is used only on the needle tip. Do not install Luer plug directly on the cover.

6. To remove any of the Luer plugs or columns, first twist the part then pull upward (see Figures 7 and 8). Pulling is straight upward is not recommended and can cause the needle tip to disengage from the cover.

7. To remove needle tips and/or stopcock valves (if used), use the ejector (see Figure 8).

Figure 4. Close-up View of Vacuum Control and Release Valve Assembly.

Figure 5. Lightly wiggle the needle tip while pushing it down in the hold. Press it with your thumb and then using the ejector handle push it hard against the hole to ensure a good contact (also see Figure 5 and Step 3 on the needle tip installation procedure).

Figure 6. Place ejector (handle position) horizontally or diagonally across two needles and press down to ensure proper vacuum seal.

IV. needle tIP InstallatIon Procedure

Attention: To achieve proper sealing between the needle tips and the

holds on the cover, this procedure requires that the cover must be placed

on the glass chamber! Not following this requirement will result in

improper needle seating that can lead to low or lost vacuum pressure and/

or damage to the three alignment posts on the underside of the cover.

Make sure that the cover is resting on the class chamber’s edges and not

on the rack.

1. Remove the rack from inside the glass chamber. Alternatively, with the rack inside the chamber, ensure that the cover’s 3 posts are in the correct alignment with the rack and are not touching/resting on the rack.

2. Align and place the cover on the glass chamber, make sure it is seating properly around the edges of the class chamber.

3. Insert a needle tip in the hold and lightly wiggle the needle while pushing it down inside the hexagon hole until it feels that the serrated portion of the needle body is seated against the corners of the hexagon shaped hold on the cover (see Figure 5). Then with your thumb, push the needle as far as it can go. Repeat the same steps, for the rest of the needle tips. The same method is also used to install stopcock valves (if used).

4. Place the ejector handle portion, horizontally or diagonally, on top of two needles and press it down hard. Repeat the same steps on all the needles (see Figure 6). It is also applied toward the installation of the stopcock valves (if used).

To Remove ColumnsEmpty Tube! Twist tube to break off contact, then pull upward.Pulling directly upward without twisting is not recommended and cancasue needle tip to disengage from the cover.

SlideTwist and

Pull UpPush Down Gently

To Remove any of the Needles/Stopcock Valves:Slide ejector under the needle tip or stopcockvalve and push ejector down.

Caution: Push down gently, pushing ejector down too fast or too hard will cause the part to bounce off in the air

Place ejector (handle portion) horizontally or diagonally across two needles and press down to assure proper vacuum seal

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Extraction Manifold

Figure 7. To remove Luer plug slowly twist the plug to break the contact, when it is loosened, then pull the plug away. To install Luer plus push it in the needle tip hole(s).

Figure 8. To remove columns, empty tube. Twist tube to break off contact, then pull upward. Pulling directly upward without twisting is not recommended and can cause needle tip is disengage from the cover. To remove any of the needle/stopcock valves, slide ejector under the needle tip or stopcock valve and push ejector down.

Needle Serated Area

Lightly wiggle the needle tip while pushing it down in the hole. Press it with your thumb and the using the ejector handle push it hard against the hole to assure a good contact (see also Figure. 2 and step number 3 on the needle tip installations procedure sheet).

Needle Insertion

Correctly Seated NeedleShowing Aligned Serations with Corners of Hexagon Hole.

Note: Picture shown are for illustration purposes only.

Hexagon Shape Hole on the Cover

Note: Luer plug is only to be used on the needle tip. Do not install luer plugs directly on the cover!

Warning: When removing plug(s), do not pull the plug staright upward, doing so can disengage the needle tip.

To Remove Luer Plug:Slowly twist the plug to break the contact,when it is loosen then pull the plug away.

To Install the Luer Plug:Push it in the needle tip hole(s).

Waters corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com

[ Care and Use ManUal ]

Waters, Oasis, and Sep-Pak are registered trademarks of Waters Corporation. T he Science of What’s Possible is a trademark of Waters Corporation. All other trademarks are property of their respective owners.

©2013 Waters Corporation. Produced in the U.S.A.June 2013 WAT200683 Rev. A KP-PDF

V. sPare Parts and accessorIes

spare Parts

description Part number

White Delrin cover, 20-position, w/o polyethylene gasket w/o gauge assembly

WAT200686

Gauge assembly, vacuum, with control and release valves

WAT200687

Gasket for 20-position cover WAT200690

Needle tip, long, with female Luer inlet, 20/pk WAT200691

Valve (stopcock), with long needle tip and female Luer inlet, 20/pk

WAT200685

Luer plugs, male 20/pk WAT058851

Ejector tool for removing needle tips from cover WAT058839

Ball ring, white rubber WAT058840

Exit valve WAT200689

Glass chamber for 20-position manifold WAT200688

complete Manifold Kits

description Part number

Waters Extraction Manifold, 20-position, complete, with rack for 13 mm x 75 mm tubes WAT200606

Waters Extraction Manifold, 20-position, complete, with rack for 13 mm x 100 mm tubes WAT300607

Waters Extraction Manifold, 20-position, complete, with rack for 16 mm x 75 mm tubes WAT200608

Waters Extraction Manifold, 20-position, complete, with rack for 16 mm x 100 mm tubes WAT200609

accessories

description Part number

Waters Extraction Manifold, 20-position, without rack

WAT200677

Rack, 20-position, for 13 mm x 75 mm tubes WAT200678

Rack, 20-position, for 13 mm x 100 mm tubes WAT200679

Rack, 20-position, for 16 mm x 75 mm tubes WAT200680

Rack, 20-position, for 16 mm x 100 mm tubes WAT200681

Reservoir, 30 cc (for Sep-Pak Plus, Light, Vac, Classic Cartridges), 40/box

WAT011390

Reservoir, 60 cc (for Sep-Pak Plus, Light, Vac, Classic Cartridges), 12/box

186005587

Adapter, male-male Luer (for Sep-Pak Classic Cartridges), 100/pk

WAT024310

Adapter (to attached reservoir to 1-, 3-, 6-cc Sep-Pak Vac Cartridges, 12/bag

WAT054260

Adapter (to attached reservoir to 12-, 20-, 35-cc Sep-Pak Vac Cartridges, 10/bag

WAT048160

Vacuum pump (110 V, 60 Hz) 725000417

Vacuum pump (220 V, 50 Hz) 725000604

Rack for 2 mL vials 186005234

Reversible vial rack for 1 and 4 mL vials WAT058871