Exploration of exotic ethno botanical potential in district Poonch … · Sardar Irfan Mehmood...

5
~ 5 ~ International Journal of Herbal Medicine 2019; 7(6): 05-09 E-ISSN: 2321-2187 P-ISSN: 2394-0514 IJHM 2019; 7(6): 05-09 Received: 04-09-2019 Accepted: 08-10-2019 Sardar Irfan Mehmood Department of Botany, Govt. Boys PGC College, Poonch Azad, Abbaspur, Kashmir, Jammu and Kashmir, India Nuzhat Shafi Department of Zoology, University Muzaffarabad Azad, Kashmir, Jammu and Kashmir, India Zeenat Jannat, Department of Zoology, University of Poonch Rawalakot Azad, Kashmir, Jammu and Kashmir, India Shabnum Shahida Department of Chemistry, University of Poonch Rawalakot Azad, Kashmir, Jammu and Kashmir, India Sajid Majeed Department of Horticulture, University of Poonch Rawalakot Azad, Kashmir, Jammu and Kashmir, India Tariq Habib Department of Botany, University of Muzaffarabad Azad, Kashmir, Jammu and Kashmir, India Corresponding Author: Sardar Irfan Mehmood Department of Botany, Govt. Boys PGC College, Poonch Azad, Abbaspur, Kashmir, Jammu and Kashmir, India Exploration of exotic ethno botanical potential in district Poonch Azad Jammu Kashmir Sardar Irfan Mehmood, Nuzhat Shafi, Zeenat Jannat, Shabnum Shahida, Sajid Majeed and Tariq Habib Abstract Azad Jammu and Kashmir is a famous natural territory with ecological zones for medicinal and aromatic plants. Especially district Poonch is blessed with wide range of climatic zones from subtropical to alpine zones which provide a home for medicinal herbs. Keeping these facts in mind twelve exotic species (Calendula officinalis L., Cynara scolymus L., Kalanchoe pinnata (Lam.) Pers., Lavendula angustifolia Mill., Mimosa pudica L. Mentha X piperita L., Melissa officinalis L. Origanum majurona L. Rosmarinus officinalis L., Stevia rebaudiana (Bertoni), Solanum muricatum Ait., Thymus vulgaris L. were acquired from (Bio Conservation Institute) NARC Islamabad and were planted in botanical repository at Govt. Boys College Campus. After successful plantation and cultivation different formulations like powder, oil, paste, infusion and extract were prepared from these medicinal plants. To check medicinal value of each species these formulation were distributed to 100 local habitants of Azad Kashmir. These investigations on exotic species in local area were continued during the last four years. These studies revealed that AJK has good adoptability for exotic species. Introduction, adoptability and ethno botanical collaboration of exotic MAPs can be proved beneficial for local peoples for curing basic health problems and for pharmaceutical developments. Keywords: adaptability, AJK, exotic flora, ethno botany, MAPs Introduction Azad Kashmir is situated between 73 o 28 o 14 East longitude and 34 o 22 o 25 North latitude. India held Kashmir is located towards East, Khyber Pakhtunkhwa in West, Punjab on South and Northern Areas on North. This state is bestowed with different ecological zones with drastic medicinal plant diversity [1] . Usage of medicinal plants in natural and allopathic medicines has tremendously increased during recent years in urban and rural parts of the world [2] . Native people of every region of world widely using plant based medicines providing a roadmap for modern drug developments in pharmaceutical industry [3] . Utilization of medicinal plants to combat different types of diseases can be traced back to human origin on earth [4] . Plants are providing clothing, food, shelter, decoration and treatments of various ailments [5] . About 20% medicinal plants have been explored for medicinal purposes up till now [6] . Domestication of plants for medicinal purposes, cosmetic usage and for forage is evident from human history [7] . Ethno botanists investigate and document information about interaction between human culture and plants. Their main focus was utilization, and management of plants in different societies [8] . In ethno botany taxonomic, morphological and ecological skills were essential to study cultural concepts about different plants [9] . Recent investigation on usage of medicinal plants revealed that about 70% Himalayan population and 75% Pakistani people depend upon medicinal plants to meet their health care requirements [10, 11] . Further it is reported that about 600 plant species are used for medicinal purposes in Pakistan [12] . A major part of population in Pakistan depending upon ethno medicines are restricted to western area due to presence of Himalaya habiting major bulk nearly 8000 of MAPs [13, 14] . The state of Jammu Kashmir is famous for its native and regional flora providing a sustainable home for exotic and indigenous plant species. Small piece of work has been done about exotic flora of state of Jammu Kashmir [15, 16] . Thirty seven genera of indigenous and alien were documented in Kashmir among them thirty seven species have been naturalized. There is gap of investigations on medicinal, ornamental, species in Kashmir [17] . Plant based industries have hedge potential in state of Jammu Kashmir due to its facilitating climato-edaphic environment [18] . Medicinal and ornamental flora in Kashmir flourish well as compare to neabouring regions of this state [19] . Shifting of plant species from indigenous areas to new areas is part of human

Transcript of Exploration of exotic ethno botanical potential in district Poonch … · Sardar Irfan Mehmood...

~ 5 ~

International Journal of Herbal Medicine 2019; 7(6): 05-09

E-ISSN: 2321-2187

P-ISSN: 2394-0514

IJHM 2019; 7(6): 05-09

Received: 04-09-2019 Accepted: 08-10-2019

Sardar Irfan Mehmood

Department of Botany, Govt.

Boys PGC College, Poonch Azad,

Abbaspur, Kashmir, Jammu and

Kashmir, India

Nuzhat Shafi

Department of Zoology,

University Muzaffarabad Azad,

Kashmir, Jammu and Kashmir,

India

Zeenat Jannat,

Department of Zoology,

University of Poonch Rawalakot

Azad, Kashmir, Jammu and

Kashmir, India

Shabnum Shahida

Department of Chemistry,

University of Poonch Rawalakot

Azad, Kashmir, Jammu and

Kashmir, India

Sajid Majeed

Department of Horticulture,

University of Poonch Rawalakot

Azad, Kashmir, Jammu and

Kashmir, India

Tariq Habib

Department of Botany,

University of Muzaffarabad

Azad, Kashmir, Jammu and

Kashmir, India

Corresponding Author:

Sardar Irfan Mehmood

Department of Botany, Govt.

Boys PGC College, Poonch Azad,

Abbaspur, Kashmir, Jammu and

Kashmir, India

Exploration of exotic ethno botanical potential in

district Poonch Azad Jammu Kashmir

Sardar Irfan Mehmood, Nuzhat Shafi, Zeenat Jannat, Shabnum Shahida,

Sajid Majeed and Tariq Habib

Abstract Azad Jammu and Kashmir is a famous natural territory with ecological zones for medicinal and aromatic

plants. Especially district Poonch is blessed with wide range of climatic zones from subtropical to alpine

zones which provide a home for medicinal herbs. Keeping these facts in mind twelve exotic species

(Calendula officinalis L., Cynara scolymus L., Kalanchoe pinnata (Lam.) Pers., Lavendula angustifolia

Mill., Mimosa pudica L. Mentha X piperita L., Melissa officinalis L. Origanum majurona L. Rosmarinus

officinalis L., Stevia rebaudiana (Bertoni), Solanum muricatum Ait., Thymus vulgaris L. were acquired

from (Bio Conservation Institute) NARC Islamabad and were planted in botanical repository at Govt.

Boys College Campus. After successful plantation and cultivation different formulations like powder, oil,

paste, infusion and extract were prepared from these medicinal plants. To check medicinal value of each

species these formulation were distributed to 100 local habitants of Azad Kashmir. These investigations

on exotic species in local area were continued during the last four years. These studies revealed that AJK

has good adoptability for exotic species. Introduction, adoptability and ethno botanical collaboration of

exotic MAPs can be proved beneficial for local peoples for curing basic health problems and for

pharmaceutical developments.

Keywords: adaptability, AJK, exotic flora, ethno botany, MAPs

Introduction

Azad Kashmir is situated between 73o28o14 East longitude and 34o22o25 North latitude. India

held Kashmir is located towards East, Khyber Pakhtunkhwa in West, Punjab on South and

Northern Areas on North. This state is bestowed with different ecological zones with drastic

medicinal plant diversity [1]. Usage of medicinal plants in natural and allopathic medicines has

tremendously increased during recent years in urban and rural parts of the world [2]. Native

people of every region of world widely using plant based medicines providing a roadmap for

modern drug developments in pharmaceutical industry [3]. Utilization of medicinal plants to

combat different types of diseases can be traced back to human origin on earth [4]. Plants are

providing clothing, food, shelter, decoration and treatments of various ailments [5]. About 20%

medicinal plants have been explored for medicinal purposes up till now [6]. Domestication of

plants for medicinal purposes, cosmetic usage and for forage is evident from human history [7].

Ethno botanists investigate and document information about interaction between human

culture and plants. Their main focus was utilization, and management of plants in different

societies [8].

In ethno botany taxonomic, morphological and ecological skills were essential to study

cultural concepts about different plants [9].

Recent investigation on usage of medicinal plants revealed that about 70% Himalayan

population and 75% Pakistani people depend upon medicinal plants to meet their health care

requirements [10, 11]. Further it is reported that about 600 plant species are used for medicinal

purposes in Pakistan [12].

A major part of population in Pakistan depending upon ethno medicines are restricted to

western area due to presence of Himalaya habiting major bulk nearly 8000 of MAPs [13, 14].

The state of Jammu Kashmir is famous for its native and regional flora providing a sustainable

home for exotic and indigenous plant species. Small piece of work has been done about exotic

flora of state of Jammu Kashmir [15, 16]. Thirty seven genera of indigenous and alien were

documented in Kashmir among them thirty seven species have been naturalized. There is gap

of investigations on medicinal, ornamental, species in Kashmir [17]. Plant based industries have

hedge potential in state of Jammu Kashmir due to its facilitating climato-edaphic environment [18]. Medicinal and ornamental flora in Kashmir flourish well as compare to neabouring regions

of this state [19]. Shifting of plant species from indigenous areas to new areas is part of human

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International Journal of Herbal Medicine http://www.florajournal.com/ history for plantation purposes [20]. Rate of introduction of

new species in new regions has increased during recent years

due economic values of plants in internationalized and

globalized world [21]. Recent investigation on exotic flora of

Kashmir revealed that it is ideal place for lavender cultivation

which is a important medicinal plant and is also part of our

study [22].

Current research work first time investigates collectively

introduction, cultivation, documentation, and ethno botanical

usage of exotic medicinal and aromatic plants in Azad

Kashmir. Ultimately creating a scientific room for all has

stake holders who have deep concern with plant oriented

medication.

Materials and Methods

All twelve species of MAPs were acquired from (Bio

Conservation Institute) NARC Islamabad and were planted in

repository and targeted areas in AJK. Then various types of

preparations (diction, paste, oil, extract) were prepared and

distributed to 100 local inhabitants. Information on the ethno

botanical properties of medicinal plants was collected from

100 local inhabitants and herbalists and all categories using

open ended and semi-structured questionnaires during months

of August 2014-Jul 2019. Ethno medicinal study was carried

out according to [23]. Standard ethno botanical methods such

as participant involvement, observation and open semi

structured interviews were used to gather the information.

Plant were identified and herbarium specimens were

deposited at Azad Jammu Kashmir Herbarium (AJKMAPH) [24, 25]. Labeled field images of plant species were taken

according to [26] for end users to resolve ambiguous taxonomic

identification. The medicinal enumerated alphabetically

names along with their respective family.

Results and Discussion

In current study 12 selected exotic species were planted and

investigated for medicinal usage. Detail descriptions

Botanical names, family, introduced name, origin, habit, part

used, medicinal usage was briefly described below (Table 1).

Twelve species were cultivated and propagated at College

Campus and each plant species showed 100% growth and

survival rates were represented in Fig: I and II. Mode of

preparations of medicine from each plant was elaborated in

Fig: III. Actual native origins of each plant species under

study were elaborated in Fig: V.

Table: I: Exotic medicinal flora used for the treatment of different diseases in Azad Kashmir Under study.

Botanical name Family Introduced local name Origin Habit Part used Medicinal usages References

Cynara

scolymus L. Asteraceae Kandara Mediterranean region H L

Hepatoprotective, lowers cholesterol level,

anti-diabetic [27, 28, 29]

Calendula

officinalis L. Asteraceae Gulasrafi Southern Europe H F

Antitumor, antispasmodic, Blood purifier,

wound healer. [30, 31, 32]

Kalanchoe pinnata

Pers. Crassulaceae Pather Chat Africa H L

Antimicrobial, anti-ulcer, Hepatoprotective

kidney stones breaker. [33, 34, 35]

Lavender

angustifolia Mill. Lamiaceae Lavender

Mediterranean

region S F & L Antimicrobial, antidepressant, Perfumery. [22, 46]

Mimosa pudica L. Fabaceae Chi Mui Tropical America,

Australia. H L & R

Anticonvulsant, arresting bleeding, skin

diseases [38, 39, 40]

Mentha x piprata Lamiaceae Sat Podina Middle East H F & L Antimicrobial, antioxidant, antiseptic,

carminative, [41, 42, 43]

Melissa officinalis

L. Lamiaceae Lemon bom Mediterranean region H F & L sedative, spasmolytic and antibacterial [44]

Origanum

majorana L. Lamiaceae Walhti katkini Cyprus H F & L

Antibacterial, antioxidant, tranquilizer,

sedative [25, 45, 46]

Rosmarinus

officinalis L. Lamiaceae Rosemary

Mediterranean, Spain,

France and Palestine. S F & L Antimicrobial, treats gout, arthritis, sciatica. [47, 48]

Solanum

muricatum Ait. Solanaceae Pipo fruit Andean South America H F

Anti-cancer, Diuretic, kidney asher, improves

immune system [49, 50, 51]

Stevia rebaudiana

(Bertoni) Asteraceae Methi Buti

Paraguay (South

America) H L

Antibacterial, noncarcingic, natural

Sweetener [52, 53]

Thymus vulgaris L. Lamiaceae Walti Chikel Mediterranean Region H L antimicrobial, antioxidant, asthma, sore

throat [25, 54, 55]

Parts used Key: L-Leaves, F-Fruits, R-Root, S-Seed, F-

Flower, B-Bark, WP-Whole plant, Rh- Rhizome, T-Tuber and

b-.

Habits Key: T-Tree, S-Shrubs, H-Herb and C-Climber.

Calendula officinalis L. Cynara scolymus L. Kalanchoe pinnata (Lam.) Pers. Lavender angustifolia Mill.

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International Journal of Herbal Medicine http://www.florajournal.com/

Mimosa pudica L. Mentha X piperita L.

Fig 1: Cultivation of exotic MAPs in targeted areas of Azad

Kashmir.

Misslia officinalis, Origanum majurona

Rosmarinus officinalis L Stevia rebaudiana Bertoni

Solanum muricatum Ait Thymus vulgaris L.

Continued Fig I

Fig: II: Cultivation of exotic MAPs at Govt. College Abbaspur Azad Kashmir.

Fig: III: mode of preparation of medicines

Fig IV: native regions of MAPs under study

Conclusion

Usage of local and exotic medicinal flora in District Poonch

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International Journal of Herbal Medicine http://www.florajournal.com/ has been commonly investigated during recent years.

Cultivation of local as well as exotic flora is not in practice in

Azad Kashmir. Imported medicinal plant materials are of poor

quality facing contaminations. In ordered to bridge these

problems present study was conducted. Current investigation

of the exotic flora bridges acquiring, cultivation, ethno

botanical documentation useful for sustainable usage of

medicinal plants and medicinal plants oriented products in

Azad Kashmir. This study is parallel to other countries where

plants are cultivated on huge scale for pharmaceutical

purposes. Ultimately live plantation and scientific

investigations will paved the way for allopathic, herbal and

homeopathic systems of medicines and cosmetic industry in

local area. This study also open the gate for better

management, practical utilization, broad execution concern

with future discoursers of exotic medicinal flora in state of

Jammu and Kashmir.

References 1. Bokhari TZ, Raja R, Younis U, Bushra E, Ummara U.

Medicinal importance of few plants from Azad Jammu &

Kashmir, Pakistan. FUUAST Journal of Biology.

2013; 3:93-99.

2. Ahmad KS, Qureshi R, Hameed Mansoor A, Farooq

Nawaz T. Conservation assessment and medicinal

importance of some plants resources from Sharda,

Neelum Valley, Azad Jammu and Kashmir, Pakistan. Int

J Agric Biol. 2012; 14(6):997-1000.

3. Jamila F, Mostafa E. Ethnobotanical survey of medicinal

plants used by people in Oriental Morocco to manage

various ailments. Journal of ethnopharmacology. 2014;

154(1):76-87.

4. Balick MJ, Cox PA. Ethnobotanical research and

traditional health care in developing countries. Medicinal

plants for forest conservation and health care. 1997;

92:12-23.

5. Khan SM, Page S, Ahmad H, Shaheen H, Ullah Z,

Ahmad M et al. Medicinal flora and ethnoecological

knowledge in the Naran Valley, Western Himalaya,

Pakistan. Journal of ethnobiology and ethnomedicine.

2013; 9(1):4.

6. Khan MPZ, Ahmad M, Zafar M, Sultana S, Ali MI, Sun

H. Ethnomedicinal uses of edible wild fruits (EWFs) in

Swat Valley, Northern Pakistan. J Ethno pharmacol.

2015; 173: 191-203.

7. Kala CP. Local preferences of ethnobotanical species in

the Indian Himalaya: Implications for environmental

conservation. Current science. 2007, 1828-1834.

8. Willie O. Siyanbola, Abiodun A, Egbetokun. Indigenous

Technologies and Innovation in Nigeria: Opportunities

for SMEs. American Journal of Industrial and Business

Management. 2012; 2(2).

9. Ali H, Qaiser M. The ethnobotany of Chitral valley,

Pakistan with particular reference to medicinal

plants. Pak. J. Bot. 2009; 41(4): 2009-2041.

10. Shaheen H, Shinwari ZK, Qureshi RA, Ullah Z.

Indigenous plant resources and their utilization practices

in village populations of Kashmir Himalayas. Pak J Bot.

2012b; 44: 739-745.

11. Shinwari ZK, Qaisar M. Efforts on conservation and

sustainable use of medicinal plants of Pakistan. Pak J

Bot. 2011; 43:5-10.

12. Hamayun M, Khan SA, Kim HY, Leechae IJ. Traditional

knowledge and ex-situ conservation of some threatened

medicinal plants of Swat Kohistan. Pak J Bot. 2006;

38:205-209.

13. Malik ZA, Bhat JA, Ballabha R, Bussmann RW, Bhatt

BA. Ethnomedicinal plants traditionally used in health

care practices by inhabitants of western Himalaya. J

Ethnopharmacol. 2015; 172:133-144.

14. Ballabha B, Chaurasia OP. Medicinal plants of cold

desert Ladakh used in the treatment of stomach disorders.

Indian J Tradit Knowl. 2009; 8:185-90.

15. Stewart RR, Nasir E, Ali SI. An annotated catalogue of

the vascular plants of West Pakistan and Kashmir. Flora

of West Pakistan. Fakhri Press, Karachi, 1972, 1-1028.

16. Ara S, Naqshi AR, Baba MY. Indigenous and exotic trees

and shrubs of Kashmir Valley. Indian J Forestry. 1995;

8:233-272.

17. Khuroo AA, Weber E, Malik AH, Dar GH, Reshi ZA.

Taxonomic and biogeographic patterns in the native and

alien woody flora of Kashmir Himalaya, India. Nordic

Journal of Botany. 2010; 28(6):685-696.

18. Zeerak NA, Wani SA. Diversity of Irises from Kashmir

Himalaya. Journal of Ornamental Horticulture. 2007;

10(2):115-118.

19. John AQ, Rather ZA, Paul TM, Neelofer. Evaluation of

ornamental bulbous plants for landscaping in temperate

regions. Journal of Ornamental Horticulture. 2007;

10(1):1-8.

20. Elton CS. The ecology of invasions by animals and

plants-Methuen & Co. Ltd., London, 1958, 181.

21. Catford JA, Jansson R, Nilsson C. Reducing redundancy

in invasion ecology by integrating hypotheses into a

single theoretical framework. Diversity and Distribution.

2008; 15:22-40.

22. Shawl AS, Kumar S. Potential of lavender oil industry in

Kashmir. J Med Aromat Plant Sci. 2000; 22:319-321.

23. Heinrich M, Ankli A, Frei B, Weimann C. Medicinal

plants in Mexico: Healers Consensus and Cultural

Importance. Social Sci Med. 1998; 47:1859-1871.

24. Martin GJ. Ethnobotany: a people and plants

conservation manual. London: Chapma Hall, 1995, 265-

270.

25. Ali SI, Qaiser M. (Eds.). Flora of Pakistan. Nos. Karachi,

1993-2015, 194-220.

26. Mehmood SI, Majeed S, Jannat Z, Habib T. Imaging

based ethno botanical studies of district Poonch, Azad

Jammu and Kashmir. International journal of herbal

medicine. 2018; 6(6):81-91.

27. Vamanu E. Antioxidant and antimicrobial activities of

ethanol extracts of Cynara scolymus (Cynarae folium,

Asteraceae family). Tropical Journal of Pharmaceutical

Research. 2011; 10(6):777-783.

28. Nazni P, Poongodi VT, Alagianambi P, Amirthaveni M.

Hypoglycemic and Hypolipidemic Effect of Cynara

Scolymus among Selected Type 2 Diabetic Individuals. P

J Nutr. 2009; 5:147-151.

29. Li H, Xia N, Brausch I, Yao Y, Ulrich F. Flavonoids

from Artichoke (Cynara scolymus L.) Up Regulate

Endothelial-Type Nitric-Oxide Synthase Gene

Expression in Human Endothelial Cells. J Pharmacol Exp

Ther. 2004; 310:926-932.

30. Kalvatchev Z, Walder R, Garzaro D. Anti-HIV activity of

extracts from Calendula officinalis flowers. Biomedicine

& pharmacotherapy. 1997; 51(4):176-180.

31. Muley BP, Khadabadi SS, Banarase NB. Phytochemical

constituents and pharmacological activities of Calendula

officinalis Linn (Asteraceae): a review. Tropical Journal

of Pharmaceutical Research, 2009, 8(5).

~ 9 ~

International Journal of Herbal Medicine http://www.florajournal.com/ 32. Ukiya M, Akihisa T, Yasukawa K, Tokuda H, Suzuki T,

Kimura Y et al. Anti-inflammatory, anti-tumor-

promoting, and cytotoxic activities of constituents of

marigold (Calendula officinalis) flowers. Journal of

natural products. 2006; 69(12):1692-1696.

33. Saleem, Ammara. In vitro antimicrobial and haemolytic

studies of Kalanchoe pinnata and Callistemon

viminalis. Int J Chem Biochem Sci. 2015; 7:29-34.

34. Pal S, Sen T, Chaudhari AKN.

Neuropsychopharmacological profile of the methanolic

fraction of Bryophyllum pinnatum leaf extract. Journal of

Pharmacy and Pharmacology. 1999; 51:313-318.

35. Jain SK, Srivastava S. Traditional uses of some Indian

plants among islanders of the Indian Ocean. Indian

journal of traditional knowledge. 2005; 4:345-357.

36. Cavanagh, HM, Wilkinson JM. Lavender essential oil: a

review. Australian infection control. 2005; 10(1):35-37.

37. 37. Lesage-Meessen L, Bou M, Sigoillot JC, Faulds CB,

Lomascolo A. Essential oils and distilled straws of

lavender and lavandin: a review of current use and

potential application in white biotechnology. Applied

microbiology and biotechnology. 2015; 99(8):3375-3385.

38. Bum EN, Dawack DL, Schmutz M, Rakotonirina A,

Rakotonirina SV, Portet C et al. Anticonvulsant activity

of Mimosa pudica decoction. Fitoterapia. 2004; 75(3-

4):309-314.

39. Kokane DD, More RY, Kale MB, Nehete MN,

Mehendale PC, Gadgoli CH et al. Evaluation of wound

healing activity of root of Mimosa pudica. Journal of

Ethnopharmacology. 2009; 124(2):311-315.

40. Hafsa A, Sakshi S, Anurag M, Rajiv G. Mimosa pudica L

(Laajvanti): An overview. Pharmacogn Rev. 2012; 6:115-

124.

41. İşcan G, Ki̇ri̇mer N, Kürkcüoǧlu M, Başer HC, Demirci

F. Antimicrobial screening of Mentha piperita essential

oils. Journal of agricultural and food chemistry. 2002;

50(14):3943-3946.

42. Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M,

Astaneh SA, Rasooli I et al. Biochemical activities of

Iranian Mentha piperita L. and Myrtus communis L.

essential oils. Phytochemistry. 2006; 67(12):1249-1255.

43. Singh R, Shushni MA, Belkheir A. Antibacterial and

antioxidant activities of Mentha piperita L. Arabian

Journal of Chemistry. 2015; 8(3):322-328.

44. De Sousa AC, Gattass CR, Alviano DS, Alviano CS,

Blank AF, Alves PB et al. Melissa officinalis L. essential

oil: antitumoral and antioxidant activities. Journal of

pharmacy and pharmacology. 2004; 56(5):677-681.

45. De Lima Marques J, Volcão LM, Funck GD, Kroning IS,

Silva WP, Fiorentini ÂM et al. Antimicrobial activity of

essential oils of Origanum vulgare L. and Origanum

majorana L. against Staphylococcus aureus isolated from

poultry meat. Industrial crops and products. 2015;

77:444-450.

46. Stamatis G, Kyriazopoulos P, Golegou S, Basayiannis A,

Skaltsas S, Skaltsa H et al. In vitro anti-Helicobacter

pylori activity of Greek herbal medicines. Journal of

ethnopharmacology. 2003; 88(2-3):175-179.

47. Leporatti ML, Ghedira K. Comparative analysis of

medicinal plants used in traditional medicine in Italy and

Tunisia. Journal of Ethnobiology and Ethnomedicine.

2009; 5(1):31-45.

48. Jarrar N, Abu-Hijleh A, Adwan K. Antibacterial activity

of Rosmarinus officinalis L. alone and in combination

with cefuroxime against methicillin-resistant

Staphylococcus aureus. Asian Pacific Journal of Tropical

Medicine. 2010; 3(2):121-123.

49. Rodríguez-Burruezo A, Prohens J, Fita AM. Breeding

strategies for improving the performance and fruit quality

of the pepino (Solanum muricatum): A model for the

enhancement of underutilized exotic fruits. Food

Research International. 2011; 44(7):1927-1935.

50. Gorbe M, Bhat R, Aznar E, Sancenón F, Marcos M,

Herraiz F et al. Rapid biosynthesis of silver nanoparticles

using pepino (Solanum muricatum) leaf extract and their

cytotoxicity on HeLa cells. Materials. 2016; 9(5):325-

333.

51. Shathish K, Guruvayoorappan C. Solanum muricatum

Ait. Inhibits inflammation and cancer by modulating the

immune system. Journal of cancer research and

therapeutics. 2014; 10(3):6-23.

52. Yadav AK, Singh S, Dhyani D, Ahuja PS. A review on

the improvement of stevia [Stevia rebaudiana

(Bertoni)]. Canadian Journal of Plant Science. 2011;

91(1):1-27.

53. Debnath M. Clonal propagation and antimicrobial

activity of an endemic medicinal plant Stevia

rebaudiana. Journal of medicinal plants research. 2008;

2(2):45-51.

54. Nikolic M, Glamoclija J, Ferreira IC, Calhelha RC,

Fernandes A, Markovic T et al. Chemical composition,

antimicrobial, antioxidant and antitumor activity of

Thymus serpyllum L., Thymus algeriensis Boiss. and

Reut and Thymus vulgaris L. essential oils. Industrial

Crops and Products. 2014; 52:183-190.

55. Hosseinzadeh S, Jafarikukhdan A, Hosseini A, Armand

R. The application of medicinal plants in traditional and

modern medicine: a review of Thymus vulgaris.

International Journal of Clinical Medicine. 2015;

6(09):635.