Experimento de Griffith - UNAMdepa.fquim.unam.mx/amyd/archivero/U5b_GeneticaMicrobiana...CYCLE...
Transcript of Experimento de Griffith - UNAMdepa.fquim.unam.mx/amyd/archivero/U5b_GeneticaMicrobiana...CYCLE...
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Transformación
Experimento de Griffith
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Transformación
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Transformación
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Conjugación
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Conjugación
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Mecanismo de conjugación
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Cepas Hfr (High frequency of
recombination)
Integración del
plásmido F para
formar la cepa Hfr
Ruptura del
cromosoma Hfr
en el origen de
la transferencia.
Formación de
diferentes cepas
Hfr
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Experimento de Hershey-Chase
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Ciclo de vida de un bacteriófago
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Transducción
generalizada
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Transducción especializada
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Recombinación genética
Intercambio físico de genes entre elementos genéticos.
•Sitio específica~Legítima. Se da en sitios específicos de las moléculas de
ADN involucradas, requiere de zonas de homología cortas.
~Ilegítima. Propiciada por elementos genéticos
transponibles, no requiere homologías.
•Homóloga
~ Sólo ocurre en zonas con alta homología
~ Permite reparar cromosomas dañados durante la
replicación (mantenimiento de información).
~ Depende de la intervención de un equipo enzimático
característico, en el que la proteína RecA (o equivalente)
es central en el proceso
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Recombinación homologa
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PCR
Reacción en cadena de la polimerasa
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Ciclo de la PCR
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Amplificación en cada ciclo de
la PCR
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Producto de la PCR
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Secuenciación de ADN
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PCR tiempo real
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Ingeniería Genética
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Clonación de genes
bacterianos en plantas para el
control de plagas
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Clonación de genes humanos
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Alimentos
transgénicos
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Regulación de la expresión
genética (operón Lactosa)