Experimental Techniques Training...

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Experimental Techniques Training Series Perfused Heart Assessment: Langendorff and Working Heart Training Course 1-Day Training Course for the Cardiovascular Scientist Friday, June 8th, 2012 (8:30 a.m. to 5 p.m.) The Institutes for Pharmaceutical Discovery 23 Business Park Drive Branford, CT 06405 is training course will focus on experimental theory, equipment mastery, and surgical skills for optimum isolated heart measurements. Relevant data including Leſt-Ventricular Pressure, Surface ECG, Preload and Aſterload Pressure, and Coronary Flow will be recorded and analyzed. Don’t miss this opportunity to observe live animal surgery demos on the widely accepted heart model. e technique allows the examination of contractile strength (inotropic effects), heart rate (cronotropic effects) and vascular effects without the complications of an intact animal model. e Isolated Heart experiment allows the researcher a valuable tool for investigations in pharmacological and physiological research without the systemic influences of other physiological processes. A panel of experts will be on hand to provide the crucial information needed to successfully install and maintain Langendorff and Working Heart Systems. Avoid common pitfalls and take the right steps to streamline your physiological data acquisition and produce valid results from day one. Maximize your data investment with powerful analysis toolsets. The A-Z of isolated hearts in a day-long intensive learning format. There is no other seminar or workshop like this anywhere! Register now. Reasons You Should Attend: • You are planning to implement your very own Working Heart or Langendorff system from the ground up. • You prefer to make smart decisions on how to adapt pre-existing equipment to accommodate an Isolated Heart System. • You recently purchased or inherited Isolated Heart equipment and are in need of a refresher course for you and/or your staff.

Transcript of Experimental Techniques Training...

Page 1: Experimental Techniques Training Seriescdn.adinstruments.com/adi-web/events/IsolatedHeart-6-8-12.pdfExperimental Techniques Training Series Training Overview: A day-long, thorough

Experimental TechniquesTraining Series

Perfused Heart Assessment: Langendorff and Working Heart Training Course

1-Day Training Course for the Cardiovascular ScientistFriday, June 8th, 2012 (8:30 a.m. to 5 p.m.)The Institutes for Pharmaceutical Discovery23 Business Park DriveBranford, CT 06405

This training course will focus on experimental theory, equipment mastery, and surgical skills for optimum isolated heart measurements. Relevant data including Left-Ventricular Pressure, Surface ECG, Preload and Afterload Pressure, and Coronary Flow will be recorded and analyzed.

Don’t miss this opportunity to observe live animal surgery demos on the widely accepted heart model. The technique allows the examination of contractile strength (inotropic effects), heart rate (cronotropic effects) and vascular effects without the complications of an intact animal model. The Isolated Heart experiment allows the researcher a valuable tool for investigations in pharmacological and physiological research without the systemic influences of other physiological processes.

A panel of experts will be on hand to provide the crucial information needed to successfully install and maintain Langendorff and Working Heart Systems.

Avoid common pitfalls and take the right steps to streamline your physiological data acquisition and produce valid results from day one. Maximize your data investment with powerful analysis toolsets.

The A-Z of isolated hearts in a day-long intensive learning format.

There is no other seminar or workshop like this anywhere!Register now.

Reasons You Should Attend:

• YouareplanningtoimplementyourveryownWorkingHeartorLangendorffsystemfromthegroundup.

• Youprefertomakesmartdecisionsonhowtoadaptpre-existingequipmenttoaccommodateanIsolatedHeartSystem.

• YourecentlypurchasedorinheritedIsolatedHeartequipmentandareinneedofarefreshercourseforyouand/oryourstaff.

Page 2: Experimental Techniques Training Seriescdn.adinstruments.com/adi-web/events/IsolatedHeart-6-8-12.pdfExperimental Techniques Training Series Training Overview: A day-long, thorough

Experimental TechniquesTraining Series

Training Overview:

A day-long, thorough overview of the Langendorff and Working Heart Systems, this training course will pair the necessary theory, surgery, equipment and software components necessary to implement successful cardiac function research studies.

With a hands-on approach to learning, the goal of this educational event is to provide attendees access to techniques not easily found through traditional channels. Since there are as many potential experimental variations as there are scientists, each day’s curriculum has been designed to introduce concepts that keep with the modern day practices but allow the scientist plenty of room for individualized application.

Using the Isolated Heart Techniques, investigations involving phenotyping, gene manipulation and pharmacological studies can be conducted without the effects of systemic intervention. Ventricular Preload and Afterload, Ventricular Pressure, Ventricular Volume, Coronary Flow, and Metabolic Parameters are investigated in the Isolated Heart experiment, providing a thorough evaluation of a wide range of heart properties.

This course is designed to provide the researcher a thorough overview of the two main Isolated Heart techniques. Experimental theory, equipment operation, and surgical techniques are combined with data collection and analysis to walk researchers through all of the skills necessary to conduct Langendorff and Working Heart experiments.

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Experimental TechniquesTraining Series

Training Course Instructors:

Jeff McVeigh — Research Scientist

Jeff has over 15 years of small animal research experience, most of which has been focused on isolated heart experimentation. While working for a Bay Area Cardiovascular Pharmaceutical Company, Jeff’s group focused on the application of molecular cardiology to the discovery and development of novel, small molecule drugs for the treatment of cardiovascular diseases. In addition, Jeff’s group was a key component of obtaining FDA approval for their Company’s compound. Jeff brings expertise in surgical techniques and vast experience in the operation

and execution of Isolated Heart Experiments. For the last 3 years Jeff has been ADInstruments training consultant for Isolated Heart Preparations.

Brandon Bucher — Product Manager, ADInstruments

As a member of ADInstruments’ Corporate Training & Support Team, Brandon has extensive experience providing hands-on training and technical support to a large customer base of cardiovascular researchers focused on Isolated Heart Experiments. Brandon’s background in biomedical engineering, and previous work in designing specialized Langendorff systems delivers an exceptional understanding of the underlying theory, mechanics, and operation of Isolated Heart systems, with a strong grasp of the necessary data collection and analysis tools that are key to the experiment. Brandon received his B.S. in Bioengineering from Washington State University (2006).

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Experimental TechniquesTraining Series

Training Sponsors:

The Institutes for Pharmaceutical Discovery, LLC (IPD), is a privately held research company founded in 1995. IPD and its affiliate institutes, the Institute for Bioanalytics, LLC (IBA) and the Institute for Drug Discovery, LLC (IDD), occupy laboratory and office space of 48,000 square feet in Branford, CT. IPD’s research team possesses core competencies in chemistry, in vitro assay development, in vivo pharmacology, drug metabolism and pharmacokinetics, and bioanalytical assay development.

Established in 1988, ADInstruments is a world-leading provider of computer-based data acquisition and analysis systems for Life Science. Their products enable users to record and analyze life science data quickly and efficiently, whether it is to further their academic or private industry research, or to advance understanding of scientific concepts in biomedical classrooms. ADInstruments’ product range is based on the world-renowned PowerLab data acquisition system with LabChart software.

PowerLab holds a leadership position in its market sector and is used in universities, hospitals, research institutes, pharmaceutical companies, and contract research organizations.

Radnoti has been setting the research standard in glassware apparatus technology and functionality for over 35 years by providing complete solutions for isolated tissue and isolated organ research. In addition to offering the highest quality products, Radnoti’s goal is to give customers an exceptional level of commitment to their satisfaction and success. Radnoti glassware and PowerLab systems provide a complete solution for isolated tissue and isolated organ research. The functional glassware provides the user with controlled conditions for conducting experiments with isolated organ and tissue preparations, while the PowerLab system records, displays and analyzes data in real time.

 

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Experimental TechniquesTraining Series

Training Agenda:

Morning Session

8:20 – 8:30 a.m.• Attendeesarrive

8:30 – 8:45 a.m.• Introductions,CourseOverview,LearningObjectives

8:45 – 10:00 a.m.

Introduction to Isolated Heart Experiments• ExperimentalTheory

• EquipmentBasics

• RecordedParameters

10:00 – 10:30 a.m.• BenefitsandLimitationsofIsolatedHeartModels

10:30 – 11:30 a.m.

Isolated Heart Equipment Overview• BasicOperation

• SystemPriming

• RetrogradePerfusioncircuit(Langendorff):ConstantPressure,ConstantFlow,RecirculationandNon-Recirculating

• WorkingHeartPerfusionCircuit

• TroubleShootingTechniques

11:30 – 12:30 p.m.

Surgical Technique Overview• AnimalConsiderations

• SurgeryOverview

• HangingTechniques

12:30 – 1:30 p.m.• Breakforlunch(provided)

Afternoon Session

1:30 – 2:30 p.m.

Introduction of the rat heart to the perfusion circuit

• LangendorffPerfusionBasics

• WorkingHeartBasics

• HeartAnatomy(landmarking)

• SystemOptimization

• AorticandLeftAtriaCannulation

2:30 – 3:30 p.m.

Transducer Configuration and Measurement• TransducerPlacement

• LeftAtriaPressure(Preload)

• AorticPressure(Afterload)

• VentricularPressure

• LeftVentriculardP/dt

• CardiacElectricalActivity

• HeartRate

• Temperature

• CardiacPacing

4:00 – 5:00 p.m.

Data analysis: from raw data to great results

• BasicsofDataAcquisition

• SettingupLabChart

• PressureAnalysis

• SignalDerivation

• dP/dt

• AutomatedAnalysisModules,ECGAnalysis,BloodPressure

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ADInstruments in Research — Training Series for Scientists

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I will be attending the Isolated Heart Experimental Techniques Workshop on June 8th, 2012 at The Institutes for

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Payment & Cancellation

Training registration is not official until you have received an ADInstruments’ invoice confirming your registration. Please do not make travel plans until you have received an enrollment confirmation from ADInstruments. Deadline for registration is 14 days prior to the event. Cancellations must be received no later than 14 days prior to the event to receive a 50% refund of the registration fee. No refunds will be issued if cancellation is received less than 14 days prior to the event.

Advanced payment in full is required to reserve your space at this event. Attendee lists will be filled on a first-come, first-serve basis in the order that payment is received. *Pricing valid for 2012 training course.

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