Experimental ostheolathyrism in rats
1
S234 Abstracts / Toxicology Letters 172S (2007) S1–S240 and pepsin secretions were measured by titration and the Anson method, respectively. Stages of the measurements were basal (first and second), stimulated and returned- basal. Results: Basal acid and pepsin secretions in the paraoxon group (14.61 ± 1.46 and 2.97 ± 0.32 g/15 min, respectively) were more than those in the control group (7.88 ± 0.26 and 0.55 ± 0.06 mol/15 min). Although pentagastrin- stimulated acid secretion in all the three groups was more than basal acid secretion, there were no differences between the groups. Stimulated pepsin secretion in the paraoxon group was significantly more than that in the control group. Conclusion: Paraoxon, in chronic exposure, increases gastric acid and pepsin secretion. doi:10.1016/j.toxlet.2007.05.587 Z32 Effects of cysteamine on in vitro maturation of mouse oocytes (IVM) in two media Parichehr Pasbakhsh 1 , Amaneh Mohammadi Roushandeh 2 , Mohammad Hosein Noori Mooghahi 1 , Mirabbas Abdolvahhabi 1 , Mohammad. Akbari 1 , Mohammad Ali Shokrgozar 3 , Aligholi Sobhani 1 1 Department of Anatomy, School of Medicine, Medical Sciences/University of Tehran, Tehran, Iran; 2 Department of Anatomy, Tabriz University, Tabriz, Iran; 3 National Cell Bank of Iran (NCBI), Pasteure Institute of Iran, Tehran, Iran Low rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. A sub- optimal environment for maturation in vitro is one of the many factors that could account for the low IVM rates. In this study, we investigated effects of supple- mentation of different dose of cysteamine on in vitro maturation of oocytes in two different media. Germinal vesicle oocytes were collected from immature mouse ovary. Germinal vesicle oocytes were cultured in two media (TCM199 and MEME) with 0, 50, 100, 200, 500 M/ml cysteamine. Number of germinal vesicle breakdown (GVBD) and metaphase II (MII) oocytes were recorded 4 and 12 h after culture, respectively. The results showed that, rate of IVM in 100 M cysteamine was high significantly compared to control groups in two media (p < 0.05). Comparison of two media showed that TCM199 improved rate of IVM and oocyte matu- ration better than MEME, however, this difference was not significant. These findings indicated that presence of cysteamine in culture medium can affect the rate of maturation of oocytes. doi:10.1016/j.toxlet.2007.05.588 Z33 Experimental ostheolathyrism in rats Ant´ onio Ramos 1 , Ant´ onio Cabrita 1 , Fernando Silva 2 1 Institute of Experimental Pathology, Fac. Medicine, University of Coimbra, Coimbra, Portugal; 2 Department of Biology, University of ´ Evora, ´ Evora, Portugal Presently, lathyrism, still an important public health sub- ject in some countries, particularly in Africa and Asia. The objectives of this study is to evaluate morphogenic changes in bone and cartilages related to this disease. Thirty animals were use randomly distributed by three groups of 10 animals each. Group I was submitted to no manipulation. Groups II and III were submitted to the administration of semicarbazide hydrochloride for 4 weeks. The toxic was incorporated in the diet, in the dose of 3 g/kg, for group II and in dose of 6 g/kg for group III. All the animals were sacrificed on the 30th day, by an overdose of anesthetics and necropsy was performed. Each tissue sample was embedded separately in paraffin block. After embeddin, cross-sections were taken from each block and stained with hematoxylin–eosin, Masson tricromic and red Syrius. The slides were examined by light microscope and the histopathologic study of bones and cartilages was performed. In tissues sections obtained from groups II and III were observed morphological alterations in the growth plate cartilage. The morphologic alterations observed suggest that semicarbazide hydrochloride have marked influences on cartilages, that is expressed by morphological alterations in growth plate cartilages. doi:10.1016/j.toxlet.2007.05.589 Z34 Predictive profiling of chemical compounds using human in vitro model systems and Omics technologies Savithri Rangarajan , Jan C.M. van der Hoeven VitrOmics BV, ’s-Hertogenbosch, Netherlands Our main objective is to build a quality-controlled database of toxic reference compounds using currently
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Transcript of Experimental ostheolathyrism in rats
Letters
S234 Abstracts / Toxicologyand pepsin secretions were measured by titration and theAnson method, respectively. Stages of the measurementswere basal (first and second), stimulated and returned-basal.
Results: Basal acid and pepsin secretionsin the paraoxon group (14.61 ± 1.46 and2.97 ± 0.32 �g/15 min, respectively) were morethan those in the control group (7.88 ± 0.26 and0.55 ± 0.06 �mol/15 min). Although pentagastrin-stimulated acid secretion in all the three groups wasmore than basal acid secretion, there were no differencesbetween the groups. Stimulated pepsin secretion in theparaoxon group was significantly more than that in thecontrol group.
Conclusion: Paraoxon, in chronic exposure, increasesgastric acid and pepsin secretion.
doi:10.1016/j.toxlet.2007.05.587
Z32Effects of cysteamine on in vitro maturation ofmouse oocytes (IVM) in two media
Parichehr Pasbakhsh 1, Amaneh MohammadiRoushandeh 2, Mohammad Hosein Noori Mooghahi 1,Mirabbas Abdolvahhabi 1, Mohammad. Akbari 1,Mohammad Ali Shokrgozar 3, Aligholi Sobhani 1
1 Department of Anatomy, School of Medicine, MedicalSciences/University of Tehran, Tehran, Iran;2 Department of Anatomy, Tabriz University, Tabriz,Iran; 3 National Cell Bank of Iran (NCBI), PasteureInstitute of Iran, Tehran, Iran
Low rate of in vitro maturation of oocytes is one of thechallenges of assisted reproductive techniques. A sub-optimal environment for maturation in vitro is one ofthe many factors that could account for the low IVMrates. In this study, we investigated effects of supple-mentation of different dose of cysteamine on in vitromaturation of oocytes in two different media. Germinalvesicle oocytes were collected from immature mouseovary. Germinal vesicle oocytes were cultured in twomedia (TCM199 and MEME) with 0, 50, 100, 200,500 �M/ml cysteamine. Number of germinal vesiclebreakdown (GVBD) and metaphase II (MII) oocyteswere recorded 4 and 12 h after culture, respectively. Theresults showed that, rate of IVM in 100 �M cysteaminewas high significantly compared to control groups in
two media (p < 0.05). Comparison of two media showedthat TCM199 improved rate of IVM and oocyte matu-ration better than MEME, however, this difference wasnot significant. These findings indicated that presence172S (2007) S1–S240
of cysteamine in culture medium can affect the rate ofmaturation of oocytes.
doi:10.1016/j.toxlet.2007.05.588
Z33Experimental ostheolathyrism in rats
Antonio Ramos 1, Antonio Cabrita 1, Fernando Silva 2
1 Institute of Experimental Pathology, Fac. Medicine,University of Coimbra, Coimbra, Portugal;2 Department of Biology, University of Evora, Evora,Portugal
Presently, lathyrism, still an important public health sub-ject in some countries, particularly in Africa and Asia.The objectives of this study is to evaluate morphogenicchanges in bone and cartilages related to this disease.
Thirty animals were use randomly distributed by threegroups of 10 animals each. Group I was submitted tono manipulation. Groups II and III were submitted tothe administration of semicarbazide hydrochloride for4 weeks. The toxic was incorporated in the diet, in thedose of 3 g/kg, for group II and in dose of 6 g/kg for groupIII. All the animals were sacrificed on the 30th day, byan overdose of anesthetics and necropsy was performed.Each tissue sample was embedded separately in paraffinblock. After embeddin, cross-sections were taken fromeach block and stained with hematoxylin–eosin, Massontricromic and red Syrius. The slides were examined bylight microscope and the histopathologic study of bonesand cartilages was performed.
In tissues sections obtained from groups II and IIIwere observed morphological alterations in the growthplate cartilage.
The morphologic alterations observed suggest thatsemicarbazide hydrochloride have marked influences oncartilages, that is expressed by morphological alterationsin growth plate cartilages.
doi:10.1016/j.toxlet.2007.05.589
Z34Predictive profiling of chemical compounds usinghuman in vitro model systems and Omicstechnologies
Savithri Rangarajan, Jan C.M. van der Hoeven
VitrOmics BV, ’s-Hertogenbosch, Netherlands
Our main objective is to build a quality-controlleddatabase of toxic reference compounds using currently
