Experiment 1 FT 131

8/7/2019 Experiment 1 FT 131

1/28


2/28

Pigment Color Before Storage Color After Storage

Chlorophyll Brownish green Greenish brown

Table 1. Color of extracts at ambient conditions

Table 2. Color of extracts subjected to heat.

Pigment Color after placement in water bath

Chlorophyll brown


3/28

Table 3. Color of extracts added with some reagents (glacialacetic acid, 5% ascorbic acid and 10% lime water)

Pigment 30 minutes 3 days

Chlorophyll Yellow green w/ precipitatae Brownish green w/ precipitate

Pigment Observation

Chlorophyll Became greener

Table 4. Color of extracts after addition of sodium bisulfite .


4/28

T ime elapsed (minutes) Color of leaves

5 Green

10 bright green

15 Yellowish green

20 Yellow

Table 5. Color of camote leaves after blanching.


5/28


6/28

Sample Used Extracted pigments Observastions

Purple cabbage Betalains The color of the

filtrate obtained

was pink (lighter

than the

anthocyanin)

Table 1. Table showing the sample used, the extracted

pigments and the initial observations

B. Stability Tests

Table 2. Table showing the observations made after one weekof storage in an ambient condition

Betalains The color of the extract was

still pink

A. Extraction


7/28

Table 3. Table showing the observations made on theeffects of heating in a water bath for thirtyminutes on the pigments

Betalains The color of the extract became

purple

Table 4. Table showing the effects of the addition of

some reagents (glacial acetic acid, 5% ascorbicacid and 10% lime water) on the extracts andthe exposure to strong light for thirty minutes

Betalains There was a presence of precipitate

and the color became purple


8/28

Table 5. Table showing the effects of the addition ofsome reagents (glacial acetic acid, 5% ascorbicacid and 10% lime water) on the extracts and its

storage in a dark place for three days

Betalains The color became pink

Table 6. Table showing the reaction of the pigments ponaddtion of Na bis lfate

Betalains The color became pinkish

p rple and more distinct


9/28

ANTHOCYANIN


10/28

Pigment Color BeforeStorage

Color AfterStorage

Table 1. Color of extracts at ambient conditions .

Anthocyanin Dark fuchsia

pink

Fuchsia pink

Table 2. Color of extracts subjected to heat .

Pigment Color after placementin water bath

Anthocyanin Dark red


11/28

Pigment 30 minutes 3 days

Anthocyanin Dark purple Dark pink

Table 3. Color of extracts added with some reagents (glacialacetic acid, 5% ascorbic acid and 10% lime water)

Pigment Observation

Anthocyanin More intense red

Table 4. Color of extracts after addition of sodium bisulfite .


12/28

CAROTENOID


13/28

L YCOPENE


14/28

Natures most widespread pigment.


15/28

Acts as secondary pigment in harvesting lightenergy.AntioxidantPrecursors of vitamin A


16/28

Carotenoids are comprised two structural groups:the hydrocarbon carotene and the oxygenatedxanthophyll .

Isoprene units linked covalently in either head-tail or tail-tail fashion


17/28


18/28

C arotene Immediate Storage

C olor of Fresh Extract Yellow orange Yellow orange (darker)

Effect of Heat Yellow orange ------

Effect of Reagent Light Yellow Light Yellow

Reaction with Nabisulfate

Light Yellow Lighter Yellow


19/28

Yellow orangeDarkening


20/28

N o changemoderately heat stable.

Even if degradation occurs color is mostlyunaffected, mostly it is the nutrition that heatinggreatly have an effect on.


21/28

Isomerization in the presence of acid.In the presence of light oxidation occurs.


22/28

Immediately changed color to yellow to lightyellow.

Oxidation occurred


23/28

said to be of especially efficient as anantioxidant


24/28

Reactions Immediate Storage

C olor of Extract Yellow Yellow orange

Effect of Heat Yellow ----

Effect of Reagents Dull Yellow Light Yellow

Effect of Na bisulfate Yellow Light Yellow


25/28

Yellow

Dark Yellow


26/28

N o changeModerately stable


27/28

Changed to dull yellow after 30 minutes instrong light.

Isomerazation .oxidation


28/28

Oxidation.Color loss

Experiment 1 FT 131

Documents

Transcript of Experiment 1 FT 131