Ex. 8 : Aseptic Technique – Inoculating Different Types of Media
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Ex. 8: Aseptic Technique – Inoculating Different Types of Media Key concepts: 1. Different types of bacterial cultures: broth, agar slant, and agar deep. 2. Aseptic transfer of bacteria from one type of culture medium to another. 3. Appropriate use of inoculating loops and needles.
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Ex. 8 : Aseptic Technique – Inoculating Different Types of Media. Key concepts: Different types of bacterial cultures: broth, agar slant, and agar deep. Aseptic transfer of bacteria from one type of culture medium to another . Appropriate use of inoculating loops and needles. - PowerPoint PPT Presentation
Transcript of Ex. 8 : Aseptic Technique – Inoculating Different Types of Media
Ex. 8: Aseptic Technique – Inoculating Different Types of Media
Key concepts: 1. Different types of bacterial cultures: broth,
agar slant, and agar deep.2. Aseptic transfer of bacteria from one type
of culture medium to another.3. Appropriate use of inoculating loops and
needles.
Four Basic Forms of Bacterial Culture Media
Agar plateBroth Agar Deep Agar Slant
Always use sterile instruments to transfer bacteria!
Loop and Needle
Flame loop before AND after using it
Other Sterile Transfer Instruments
“Little Finger Technique”
NA Broth
NA Slant Cultures
Agar Deeps: What is happening?
Use the Inoculating Needle to inoculate a deep
