Evolution Theory,Metabolic Disease

And Aging

Roadmap to Metabolic Health and Anti-Aging with Accelerated Amino Acid

Delivery Technologies (AAADT)

George A. Scheele, M.D.

American Health Plummets

• 1950: American men & women first• 2012: American men last and women next to

last• The US ranked near bottom in almost every

health indicator– Highest obesity rate– Highest diabetes rate– 2nd highest heart disease rate

In 2012 the Institute of Medicine Studied Life-Expectancy in 17 Wealthy Countries:

First to Last in Health in 62 years

The Perfect Storm

• A Food Crisis• A Disease Crisis• An Aging Crisis• An Economic Crisis

Four Crises Encircling the Globe:

Diet-Related Disorders Cost $2 Trillion

Obesity & Metabolic Syndrome

• Heart Disease• Type 2 Diabetes• Hypertension• Stroke• Cancer• Other medical conditions

Obesity Associates With Chronic Degenerative Disease:

Two Epidemics: Obesity & Metabolic Disease

Epidemic of Metabolic DiseaseNumber of Cases in the US alone:

Disease IncidenceOverweight disorders 150 million

Obesity 75 million

High blood pressure 34 million

High cholesterol & TGL 30 million

High blood sugar 90 million

Type 2 Diabetes 30 million

Cardiovascular disease 57 million

Autoimmune disease 50 million

Cancer 18 million

Osteoarthritis 20 million

Gallstones 19 million

America Challenged with Chronic Degenerative Disease

Essential Amino Acids

Hypothesis: Essential Amino Acids (EAA) Drive the Success of:

• Weight Loss Health• Metabolic Health• Aging• Brain Health

Essential AA the “Essence” of Good Health

Selective Protein Deficiency Syndrome

2-Dimensional Gel Analysis Invented by Dr. Scheele in 1975:

A: Health Normal Protein Pattern Normal body weight

B: Selective Protein Deficiency Syndrome Positive Charged Proteins Reduced by 90% Within 12 days (gain in body weight)

C: CORRECTION with Factor4 Weight Control® Power Amino Acids® correct protein deficiency (Body weight returns to normal)

Low Protein Diets Cause Protein Deficiency

Maintain Your Protein Team To Achieve Health

Loss of Positive-Charged ProteinsPancreatic

EnzymeIEP High Protein Diet1

(82%)Low Protein Diet1

(0%)Trypsinogen 1 4.3 1.29 1.86Trypsinogen 2 4.4 1.75 2.81Procarboxypeptidases 4.3-4.6 1.31 1.28Chymotrypsinogen 1 4.8 1.62 1.41Proelastase 4.9 2.76 2.20Lipase 6.8 0.74 0.23Trypsinogen 4 8.0 1.06 0.36Amylase 1 8.6 0.36 0.41Amylase 2 8.9 0.13 0.19Proelastase 2 9.1 0.56 0.36Chymotrypsinogen 2 9.2 1.64 0.57Ribonuclease 9.3 0.90 0.47

1 Fractional rates of synthesis (FRS) of indicated proteins after 12 days on the indicated diet divided by the corresponding FRS after control (22% protein) diet. Studies conducted in rats.Conclusions:

• In the high-protein, low-carb diet the synthesis of protease enzymes increase.• In the zero protein, high-carb diet, positive-charged proteins with a high IEP & KR

value disappear.

2D Gel Analysis of Dietary Studies

Provided the Following New Discoveries:

1. Dietary Cause of Selective Protein Deficiency

2. Dietary cause of an imbalance in metabolic pathways

3. The vulnerable shoulder of the food chain

4. The role of diet in the epigenetics of metabolic disease

Link Between Diet and Protein Deficiency

Role of EAA in Hunger & Satiety

• EAA regulate hunger & satiety in the Arcuate Nucleus, the appetite center of the brain

• EAA regulate hunger & satiety in the GI tract through Serotonin

• EAA satiety signals can be enhanced Composition-dependent Dose-Dependent Metabolite-dependent

• EAA ensure that the organism is well fed, which promotes the life cycle & reproduction

Essential Amino Acids (EAA) Fundamental to the Evolution of Vertebrates, Mammals and Humans:

Evolution Concerned with Feeding & Procreation

Accelerated Amino Acid Delivery

Accelerated Amino Acid Delivery Technologies (AAADT) Enhance the Delivery of EAA to the Body

Free EAA added to Protein Accelerates Amino Acid Delivery by 1 to 3 Orders of Magnitude through Synergistic Mechanisms:

• AAD is accelerated more than 10-fold over the 4-6 hour ingestion cycle

• AAD is accelerated more than 100-fold over the first 90 minutes

• AAD is accelerated more than a thousand-fold over the first 30 min.

Accelerated Amino Acid DeliveryGraphic Representation:

Accelerated Amino Acid Delivery Technologies (AAADT) Enhance the Delivery of EAA to the Body

Poor Assimilation of Protein Powders/Meals

Dietary Balance Studies Conducted by the International Nutrition Research Center (INRC) in Coral Gables:

Conclusion: Limited digestion & absorption of protein powders & meals without addition of free amino acids

Meal Absorbed WasteProtein Powder 17% 83%Fish 33% 67%Poultry 33% 67%Meat 30% 70%Egg Whites 49% 51%

Consistent With the Absence of AAADT Synergies

Role of EAA in Digestive Health

• Free EAA rapidly access the arcuate nucleus within 10 minutes providing initial & sustained satiation

• Free EAA stimulate CCK and secretin release in the GI Tract CCK & secretin stimulate production of

pancreatic enzymes CCK & Secretin stimulate pancreatic enzyme

secretion CCK stimulates gallbladder contraction CCK enhances protein digestion & amino acid

absorption CCK further increases satiation

Free EAA Provided With Backup Protein:

AA Delivery Increased 1 to 3 Orders of Magnitude

Role of EAA in Metabolic Health

Essential, Positive-Charged & Satiety Amino Acids (Power Amino Acids®) Achieve the Following:

• Appetite is reduced• Essential calories delivered to body• Selective protein deficiency relieved• Metabolic pathways balanced

Body Nourished & Metabolic Syndrome Tamed

AAADT Protected by Patents

Accelerated Amino Acid Delivery Technologies (AAADT) Protected by Issued Patents:

• Utility Patent: High Protein Supplement, US Patent No. 7,982,066 B2 (7-19-11)

• Composition of Matter Patent: High Protein Supplement, Methods of Delivery: US Patent No. 8,362,297 B2 (1-29-13)

• Methods of Delivery Patent: US Patent No. 8,779,189 B2 (7-15-14)

Factor4 & AAADT Patents Issued through 2033

Solid Scientific ResearchEstimated $50 Billion of NIH Support Over 50 Years in Digestive and Metabolic Health:

The Obesity Cure Connects the DotsFor The Clinical Breakthrough

The Basic Science

The Cure

The Product

Health & Wellness Innovation at its Best

* Claims have not been reviewed by the FDA

Power Amino Acids®

• Essential amino acids• Positive-charged amino acids• Satiety amino acids

Free Amino Acids Absorbed Early (10-90 minutes):

Protein-Bound Amino Acids Absorbed Later (1-4 hours):

• Essential amino acids (9)• Non-essential amino acids (11)

Accelerated Amino Acid Delivery Technology

Synergy Between Amino Acids and Protein:

• Power Amino Acids increase protein digestion & Amino acid absorption more than 10-fold

Weight Loss Study

• History: 25 subjects with Chronic overweight disorders for 20-30 yrs. (historical controls)

• Within 3 months 96% of subjects lost weight• Weight loss results:

Weight Loss Significance:FDA Requires a 5% Loss in Body Weight in 12 Months

Conducted in 2007:

Endpoints 3 months 6 months 12 monthsWeight loss in pounds

8.4 lbs 16.2 lbs 33.4 lbs

% body weight loss

4.5% 8.2% 16%

Bottom Line

Exploit nature’s feedback mechanisms to:

• Suppress appetite to avoid food cravings• Burn fat to reduce excess body weight• Build muscle to improve body definition• Boost energy to tackle life• Combat metabolic disease & premature aging

Power Amino Acids®

Scientific Research Reveals a New & Better Approach to Metabolic Health & Aging Based on >5,000 patients

Accelerated Amino Acid Delivery Technology (AAADT)

The Metabolic Solution

Bon Voyage to Normal Body Weight, Metabolic Health, and Anti-Aging:Power Shakes & Smoothies

Containing Power Amino Acids® And Protein

Look Younger, Feel Better & Live LongerWith Factor4 Health & AAADT

www.factor4health.com

Factor4 Health Combats DiseaseFactor4 Prevents and Treats Metabolic Diseases & Aging by Rebalancing Metabolic Pathways in:

• Obesity• Heart Disease• Type 2 Diabetes• Hypertension• Stroke• Cancer• Other Medical Conditions…

Factor4 Drives Epigenetic Changes in Dietary HealthBy Relieving Selective Protein Deficiency

KR Value: Nutrient Density

KR Value Defined as the Ratio of Positive-Charged over Negative-Charged Amino Acids (K+R/D+E):

• KR Values determine the Isoelectric Point of individual proteins• The KR Value represents the nutrient density of specific proteins• The KR Value links the nutrient value of a protein to its

vulnerability in the food chain• The KR value also links the nutrient value of proteins to the

potential for causing Metabolic disease• Factor4 Products increase the KR value of the food chain and

support health by relieving Selective Protein Deficiency Syndrome• KR values are at the heart of Natural Selection & Evolution Theory

The KR Value Links the Vulnerability of Proteins to Dietary Deficiencies in the Food Chain

Query the Human ProteomeIdentify the High KR Proteins that May Explain Metabolic Disease & Aging:

• Obesity: Lipases (HL, LPL, HSL)• Diabetes: FOX01 enhancers• Lipids: High Density Lipoproteins• Cancer: DNA-Interacting Enzymes• Dementia: REST; BDNF• Alzheimer’s Disease: APH & Pen2 subunits

for Gamma Secretase• Aging: Telomerase (TERT, DKC1, TEP1)

Putative Links Established Between Specific High-KR Proteins and Metabolic Disease & Aging

Factor4 Health & Aging

Telomere Science:• Telomeres are stretches of DNA that cap the ends of chromosomes• Cell Division shortens telomeres• Telomerase lengthens telomeres• Science has linked short telomeres with (i) heart disease, (ii)

diabetes, and (iii) cancer

Factor4 Health Postulated to:• Increase telomerase levels through promoting positive-charged

subunits, TERT, DKC1, TEP1, all showing high K+R values• Increase telomere length• Restore cell division and organ regeneration• Increase longevity

Factor4 Promotes AAADT & Healthy Aging

Factor4 Health & Brain DiseaseDementia & AD Mystery: Nutritional Factors May Negate Effects of Amyloid Plaque:

Neurotropic Proteins Combat Brain Disease:• Neuronal growth, protection, survival, differentiation

& synaptogenesis with two neurotropic proteins:REST, a nuclear proteinBDNF, a secretory protein

• REST & BDNF both show high K+R values (positive-charge)

• High KR values confer nutrient sensitive status on protein expression and function

Prediction: Dementia & AD Will Respond to the High Nutrient Value of Factor4 Health Products

SummaryBreakthrough in Metabolic Disease and Aging:

Factor4 Health® Products represent a breakthrough in Accelerated Amino Acid Delivery Technologies (AAADT) that have profound benefits in Metabolic Disease and Aging including:

• Obesity• Type-2 Diabetes• Lipid Disorders• Cardiovascular Disease• Stroke• Cancer• Other Medical Conditions

Factor4 Health® May Drastically Reduce Health-Care Costs and Dramatically Increase Healthspan & Lifespan

Learn More on Our Blog:

Link: https://blog.factor4health.com/articles/

Contents:• Introduction: Factor4 Health® Improving Lives

Across the Span of Medicine• 12 Medical Articles on Metabolic Diseases Improved

with Factor4• 6 Articles Showing the Scientific Journey over the

past 6 years• 1 Clinical Trial (2007 Weight Loss Study)• 2 Media articles

Factor4 Health® Improves Metabolic Disease and AgingThrough Accelerated Amino Acid Delivery Technologies

Nonprofit Organization

• Improve World Health• Conduct Clinical Trials• Promote education & advocacy• Mine the human proteome for high-

KR value proteins

501c3 Institute to:

Mission: To Improve World Health

Dr. George A. Scheele, Creator of Factor4 Health, to combat Metabolic Disease & Aging:

• Board Certified in Internal Medicine• Princeton BA & John Hopkins MD• Professor at Rockefeller University, Yale

University School of Medicine and Harvard Medical School.

• Two time Nobel Associate (1974 & 1999)• First to figure out how to separate all the proteins in the human body (est. 20,000) by inventing 2D gel electrophoresis

“As a medical doctor with over 40 years of research, my personal mission is to improve Metabolic Health and Aging. That is why I created Factor4 Health.”

– George A. Scheele, MD

Meet Dr. Scheele