Evolution in a changing environment: the genetic architecture of ... in a changing enviro… ·...
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Evolution in a changing environment: the genetic architecture of adaptation outside centers of domestication of Phaseolus vulgaris and P. coccineus
Roberto Papa
Università Politecnica delle Marche, Ancona
FYI - FEFAC, COCERAL and Copa-Cogeca event on Leguminoses in the framework of the international year of pulses on 29th November 2016 in
the European Parliament
GOL(16)10445
Phaseolus vulgaris L.
Independent
domestications
Mesoamerican
gene pool
Northern Peru
and Ecuador
Andean
gene pool
From northern Mexico to Northwestern Argentina;
Altitudes ranging from 500 to 2000 m. asl;
Rainfall from 500 to 1800 mm.
Evolution in a changing environment: the
genetic architecture of adaptation outside
centers of domestication of P. vulgaris and P. coccineus. Coordinator R. Papa, UNIVPM
GbS on 11500 AccessionsResequencing 560 genotypes
Transcriptomics and Metabolomics Population genomicsAdmixture mapping
GWAS
Genes for adaptation
Deep genomic and phenotypic characterization of the whole collection and Haplotype reconstruction (BeanHapMap)
10.000
500
200
GbS 10,000 pure lines from landraces (Pv_ALL)
Phenotypes MLFT
ACTIVITIES and OUTCOMES
single seed purified accessions50% from America and50% from Europe
Phenotypes controlledconditions
Metabolomics
Transcriptomics
WGS 10x
• An immortal collection of pure lines in P. vulgaris
• Complete molecular and phenotypic characterization
and Computed haplotypes for all the collection
• Genes controlling adaptation (signature of selection, traits-
environmental variable association, expression analysis, validation of
candidates genes)
MAIN DELIVERABLES
expanding the scope and the impact
ASSOCIATED PARTNERS
Collaborative network
cooperation and association to specific activities
Phenotyping (field trials, seed traits, nutritional value, abiotic and biotic stress, roots)
Genetic resources and germplasmGenomic information and molecular data
DisseminationEx situ conservation of 10,000 pure lines
…