Everyday EPRFree Radicals, Captured

Advantages of Bruker

EPRInnovation with Integrity

EPR: Seeing the Unseen EPR (Electron Paramagnetic Resonance) spectroscopy is the only technique that unambiguously detects and quantifies species with unpaired electrons. These include free radicals, many transition metals, and defects in materials.

Free radicals and transition metals play crucial roles in many processes such as photosynthesis, oxidation/reduction, catalysis, and polymerization reactions. As a result EPR crosses many disciplines such as medical science, biology, chemistry, physics, material science, archeology and forensics.

Most free radicals have damaging effects on their surroundings. Free radicals are involved in cancer, aging, Alzheimer’s, and cardiovascular diseases. Degradation of material properties such as brittleness and discoloration of polymers is frequently caused by free radical reactions.

Versatile and Nondestructive

EPR can be used to detect unpaired electrons in solids, liquids, gases, colored and turbid solutions, cell suspensions, and in vivo.

The EPR data can be acquired in a matter of seconds and the analysis of the data provides the amount and identity of the species present.

EPR: Seeing the Unseen Why Bruker EPR?With more than 50 years of experience, Bruker has built a sub-stantial rapport with the scientific community by understanding and meeting the needs of our users. With more than 90 loca-tions around the globe, we are the world’s leading supplier of EPR spectrometers.

Our EPR product family ranges from a sophisticated, compact bench-top model to complex floor-standing systems, serving the widest range of customer needs.

Bruker‘s Everyday EPR Portfolio

The Best EPR Technology- from the Experts to You

Bruker provides the highest value for your investment. Only Bruker offers expandable instrumentation to accommodate the changing needs of your research and laboratory. With a foot-print and price to fit your lab, we have exactly what you require in an EPR spectrometer: high precision, reliability and intuitive operation.

Accessible Technology�� Customizable and convenient operation�� Compact and robust instruments with minimal system size, easy installation, and low cost of operation�� Expandable with optional software and hardware packages to fit the needs of your lab

Highest Qualilty Standards�� Constructed to deliver optimum performance and superior reliability�� Every instrument thoroughly tested before leaving produc-tion facilities�� Comprehensive customer service providing sales, applica-tions, and engineering support for all Bruker products

Focused on Ease-of-Use�� Minimal sample preparation required�� Unambiguous detection of unpaired electrons in free radi-cals and metal complexes�� Quantitative and precise analysis�� Samples from gases to liquids to solids analyzed on one instrument

Streamlined Data Analysis�� Supported by dedicated work flows�� Software with predefined experiments�� Ready-to-use analysis and reporting tools

Everyday EPR Applications

SMALL MOLECULE STRUCTUREAND QUANTIFICATION

Discover the kinds of radicals in your sampleQuantify and evaluate their effects on the properties of interestStudy free radical reactions and kinetics

MACROMOLECULAR STRUCTURE

Identify metal centers, ligands and substrate binding in proteins, DNA and RNADetermine structural conformationObserve electron transfer pathways and amino acid based radicals in protein

CHEMICAL & MOLECULARDYNAMICS

Observe molecular motion e.g. dynamics of protein-lipid interactionsDetermine conformational changes of membrane proteinsUnderstand kinetics of chemical reactions

ROUTINE ANALYSIS

Determine shelf life and antioxidant level in food and beveragesObtain accurate dose measurements from irradiated materialsObserve engine oil degradation

MATERIALS

Study polymers, paints, optical fibers, batteries, solar cells, etc.Explore molecular magnetismAnalyze the effects of additives on material properties

IMAGING

See inside living organismsAccurately determine oxygen concentration in tissuesObserve the characteristics of catalytic systems

How EPR Works

A step-by-step look inside EPR from sample to analysis

Step 1: Samples are placed in a probehead often called a cavity or resonator.

Step 3: Microwave radiation at a given frequency excites some of the unpaired electrons to the higher energy level.

Step 4: The magnetic field is changed such that the energy level separation is matched by the microwave frequency.

Step 2: When an external magnetic field is applied, the unpaired electrons orient parallel or antiparallel to the direction of the magnetic field creating two distinct energy levels.

Step 5: This condition produces an EPR resonance line providing information about the unpaired electrons and their surroundings

Advantages of Bruker

Microwave Technology AdvantageMicrowave Components with Unparalleled Performance

At the heart of every EPR spectrometer are the microwave components. Our expertise in microwave technology has lead to the highest EPR sensitivity available.

Magnet Technology AdvantageMagnet Platforms with Superior Quality

The magnet performance is critical to resolution and stability. Through our decades of experience in magnet design and construction, we ensure the highest quality electromagnets for EPR.

Software AdvantageDedicated Workflows

Bruker’s hardware is complemented by comprehensive and easy-to-use software packages, making EPR accessible. We provide the tools to identify and to determine the concentration of paramagnetic species in the sample.

Service and Application AdvantageReliable Trustworthy Support

Bruker offers training courses worldwide. Skilled engineers explain the basics of all instruments. Application experts guide you through setting up experiments and interpreting results in an easy and straightforward way. Bruker specialists are only a call or email away, ready to help your lab leverage the power of EPR.

We do more! Please visit us at www.bruker.com/epr©

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