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Evaluation of Neural Tissue Regeneration Mediated …..., Sang Wha Kim. M.D., PhD. 2 1 Department of...
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Video gait analysis <Four different time points> 1) Initial contact :the moment the experimental side foot touches the ground 2) Midstance : the moment the contralateral foot in the air crosses the experimental side foot bearing the animal’s weight 3) Toe-off : the moment of maximal plantar flexion of the experimental side ankle joint 4) Midswing : the moment the experimental side foot crosses the contralateral foot Segmental nerve defects pose a daunting clinical challenge, as peripheral nerve injury studies have established that there is a critical nerve gap length for which the distance cannot be successfully bridged with current techniques. Construction of a neural conduit the peptide was effective in promoting cell attachment and spreading in vitro. The object of this study was to examine the effects of Ln2-P3 in peripheral nerve regeneration in segmental nerve defects in a rat sciatic nerve injury model.. . Methods Background Results Conclusion To examine the effects of Ln2-P3 in peripheral nerve regeneration in vivo, we used collagen tube as an artificial nerve graft. The novel graft was coated with Ln2-P3 and used to bridge a 10 mm defect in rat sciatic nerves. Control groups were treated with serum only-filled conduits of scrambled peptide coated conduits. Animals were assessed for the histopathologic examination , motor function using sciatic functional index, and video gait analysis. Animal and Experimental design - Group 1 (n=8) : normal nerve - Group 2 (n=8) : transection - Group 3 (n=8) : transection collagen tube - Group 4 (n=8) : transection collagen tube + non-functional peptide - Group 5 (n=8) : transection collagen tube + functional peptide The addition of peptide to the nerve conduit enhanced the regeneration of myelinated axons from the nerve stump compared a serum-only filled conduit and scrambled peptide conduit. These findings indicate that Ln2-P3, combined with collagen conduit, has potential biomedical applications in peripheral nerve injury repair. Evaluation of Neural Tissue Regeneration Mediated by a Functional Peptide, Ln2-P3 Youn Hwan Kim, M.D., PhD. 1 Hahn Sol Bae, M.D. 2 , Sang Wha Kim. M.D., PhD. 2 1 Department of Plastic and reconstructive Surgery, College of Medicine, Hanyang University, Seoul, Korea 2 Department of Plastic and reconstructive Surgery, College of Medicine, Seoul National University Hospital, Seoul, Korea Video gait analysis Sciatic functional index (SFI) Group1 Group2 Group3 Group4 Group5 -40 -30 -20 -10 0 2week 4week 8week SFI H&E stain Group 1 Group 3 Group 4 Group 5 IHC stain : Myelin Group 1 Group 3 Group 4 Group 5 Sciatic functional index (SFI) Footprints evaluated for three different parameter (1)Print length (PL) : distance from the heel to the third toe (2) Toe spread (TS) : distance from the first toe to the fifth toe (3) Intermediate toe spread (ITS) : distance from the second toe to the fourth toe Figure 1. Using collagen tube as an artificial nerve graft Figure 2. Evaluation of Sciatic Functional Index Figure 3. Four different time points during the gait cycle Pair-wise comparisons P value 2weeks Group 1 vs Group 5 0.021 4weeks Group 1 vs Group 4 0.020 Group 1 vs Group 5 0.014 (a) (b) Figure 4. Sciatic functional index. (a) The data of SFI. (b) comparison between groups Pair-wise comparisons P value 4weeks Group 1 vs Group 4 0.014 Group 1 vs Group 5 0.026 (a) (b) Figure 5. Video gait analysis. (a) The data of four time points (b) comparison between groups IHC stain : Neurofilament Group 1 Group 3 Group 4 Group 5
Transcript of Evaluation of Neural Tissue Regeneration Mediated …..., Sang Wha Kim. M.D., PhD. 2 1 Department of...
Video gait analysis
<Four different time points>1) Initial contact :the moment the experimental side
foot touches the ground2) Midstance : the moment the contralateral foot in the
air crosses the experimental side foot bearing the animal’s weight
3) Toe-off : the moment of maximal plantar flexion of the experimental side ankle joint
4) Midswing : the moment the experimental side foot crosses the contralateral foot
Segmental nerve defects pose a daunting clinical challenge, as peripheral nerve injury studies have established that there is a critical nerve gap length for which the distance cannot be successfully bridged with current techniques. Construction of a neural conduit the peptide was effective in promoting cell attachment and spreading in vitro. The object of this study was to examine the effects of Ln2-P3 in peripheral nerve regeneration in segmental nerve defects in a rat sciatic nerve injury model..
.
Methods
Background Results
Conclusion
To examine the effects of Ln2-P3 in peripheral nerve regeneration in vivo, we used collagen tube as an artificial nerve graft. The novel graft was coated with Ln2-P3 and used to bridge a 10 mm defect in rat sciatic nerves. Control groups were treated with serum only-filled conduits of scrambled peptide coated conduits. Animals were assessed for the histopathologic examination , motor function using sciatic functional index, and video gait analysis.
Animal and Experimental design
- Group 1 (n=8) : normal nerve - Group 2 (n=8) : transection - Group 3 (n=8) : transection collagen tube - Group 4 (n=8) : transection collagen tube + non-functional peptide - Group 5 (n=8) : transection collagen tube + functional peptide
The addition of peptide to the nerve conduit enhanced the regeneration of myelinated axons from the nerve stump compared a serum-only filled conduit and scrambled peptide conduit. These findings indicate that Ln2-P3, combined with collagen conduit, has potential biomedical applications in peripheral nerve injury repair.
Evaluation of Neural Tissue Regeneration Mediated by a Functional Peptide, Ln2-P3 Youn Hwan Kim, M.D., PhD. 1 Hahn Sol Bae, M.D.2, Sang Wha Kim. M.D., PhD.2
1Department of Plastic and reconstructive Surgery, College of Medicine, Hanyang University, Seoul, Korea2 Department of Plastic and reconstructive Surgery, College of Medicine, Seoul National University Hospital, Seoul, Korea
Video gait analysis
Sciatic functional index (SFI)
Group1
Group2
Group3
Group4
Group5
-40
-30
-20
-10
02week4week8week
SFI
H&E stain
Group 1 Group 3
Group 4 Group 5
IHC stain : Myelin
Group 1 Group 3
Group 4 Group 5
Sciatic functional index (SFI)
Footprints evaluated for three different parameter
(1)Print length (PL) : distance from the heel to the third toe
(2) Toe spread (TS) : distance from the first toe to the fifth toe
(3) Intermediate toe spread (ITS) : distance from the second toe to the fourth toe
Figure 1. Using collagen tube as an artificial nerve graft
Figure 2. Evaluation of Sciatic Functional Index
Figure 3. Four different time points during the gait cycle
Pair-wise comparisons P value
2weeks Group 1 vs Group 5 0.021
4weeks Group 1 vs Group 4 0.020
Group 1 vs Group 5 0.014
(a)
(b)
Figure 4. Sciatic functional index. (a) The data of SFI. (b) comparison between groups
Pair-wise comparisons P value
4weeks Group 1 vs Group 4 0.014
Group 1 vs Group 5 0.026
(a)
(b)
Figure 5. Video gait analysis. (a) The data of four time points (b) comparison between groups
IHC stain : Neurofilament
Group 1 Group 3
Group 4 Group 5
