Ethephon Effect - ISTA · Figure 2 Percent fresh seed of cv. KK 84-7 peanut seed after pretreatment...
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Abstract Figure 2 Percent fresh seed of cv. KK 84-7 peanut seed after pretreatment with 0.96% Ethephon and preheat at 40C for 168 h compared to untreated seed (control) 1 Papassorn Wattanakulpakin, Kantima Thongsri, Supalak Sattayasamitsathit and Jira Suwanprasert Phitsanulok Seed Research and Development Center, Department of Agriculture, 813 Moo. 8, Wangthong District, Phitsanulok 65130, THAILAND. 1 Corresponding author, Email: [email protected] Material and Method Figure 1 Germination (% Normal seedling) of cv. KK 84-7 peanut seed after pretreatment with 0.96% Ethephon and preheat at 40C for 168 h compared to untreated seed (control) Dormancy is undesirable character for the large seeded peanut (Arachis hypogeae). Khon Kaen 84-7 is one of popular peanut variety for food consumption in Thailand belonging to the Virginia type. Dormant seed of cv. KK 84-7 has existing over two months after harvesting. As the ISTA rules, preheat at 40C is recommended for breaking peanut seed dormancy but the duration is not limited depended on their varieties. The germination analysis of peanut seed is normally taken for 10 days. If breaking dormant seed is needed, the time for germination test is totally taken 17 days including preheat time. Therefore, the more rapid way and precise method in order to reduce analysis time would be studied and will be benefit for earlier issue seed certificate. Ethephon, ethylene liberation, is plant growth regulator that has been reported to break seed dormancy. The comparison between preheat and Ethephon for breaking peanut seed dormancy were studied in this experiment. Peanut seeds were preheated at 40C for 168 h (7 days), and another seed group was directly mixed with 0.96% Ethephon. All pretreated seeds were then planted according to standard germination test, compared with the no pretreated seed (control). The result found that 0.96% Ethephon was the most benefit to release dormant seed at fresh harvest and achieved 86% emergence with only 5% fresh seed. The normal seedlings and fresh seeds of preheat at 40C for 168 h was 75% and 11%, respectively. Only 6% normal seedling observed in the control and most of seed were still dormant (89% fresh seeds). After storage at 20C for 28 days, the great performances of normal seedlings was observed both Ethephon and preheat method which showed over 90% normal seedlings and 0-1% fresh seed. While, 42% normal seedlings and 32% fresh seeds were found in control. The experiment suggests that 0.96%Ethephon is the advantage method to introduce for breaking dormancy of peanut seed cv. KK 84-7 because it can release dormant peanut seed since fresh harvest. Moreover, the duration for germination test is more rapidly (only 10 days) due to preheat for 168 h is not necessary. Keywords: Peanut seed, Dormancy, Ethephon Conclusion Result 0.96% Ethephon is the advantage pretreatment for breaking peanut seed dormancy according to quick and save cost compared to preheat. This method is taken only 10 days including germination test, while preheat at 40C is extended up to 168 h or 7 days before germination test. Ethephon Effect On Peanut Seed Dormancy Release Pod seed were stored at 20C for 28 days Fresh harvested peanut seeds cv. KK 84-7 were immediately processing as drying, cleaning, and then carried out to the lab for experiment. Sampling randomly at 0, 7, 14, 21, 28 days after storage Pretreatment by Breaking seed dormancy treatments were applied. Germination test by sand method 1. Untreated seed 2. 0.96% Ethephon 3. Preheated at 40C for 168 h cv. KK 84-7 Breaking seed dormancy Control Planting Seedling evaluation Data record & Analysis 0 10 20 30 40 50 60 70 80 90 100 0 7 14 21 28 Germination (%) Days after storage at 20C Control 0.96% Ethephon Preheat at 40°C, 168 h 0 10 20 30 40 50 60 70 80 90 100 0 7 14 21 28 Fresh seed (%) Days after storage at 20C Control 0.96% Ethephon Preheat at 40°C, 168 h Normal seedling Fresh seed a b c a b c a b c a b c a a b a b c a b b a b c a b b a b b
Transcript of Ethephon Effect - ISTA · Figure 2 Percent fresh seed of cv. KK 84-7 peanut seed after pretreatment...
Abstract
Figure 2 Percent fresh seed of cv. KK 84-7 peanut seed after pretreatment with 0.96% Ethephon and preheat at 40C for 168 h compared to untreated seed (control)
1Papassorn Wattanakulpakin, Kantima Thongsri, Supalak Sattayasamitsathit and Jira SuwanprasertPhitsanulok Seed Research and Development Center, Department of Agriculture,813 Moo. 8, Wangthong District, Phitsanulok 65130, THAILAND.1Corresponding author, Email: [email protected]
Material and Method
Figure 1 Germination (% Normal seedling) of cv. KK 84-7 peanut seed after pretreatment with 0.96% Ethephon and preheat at 40C for 168 h compared to untreated seed (control)
Dormancy is undesirable character for the large seeded peanut(Arachis hypogeae). Khon Kaen 84-7 is one of popular peanut varietyfor food consumption in Thailand belonging to the Virginia type.Dormant seed of cv. KK 84-7 has existing over two months afterharvesting. As the ISTA rules, preheat at 40C is recommended forbreaking peanut seed dormancy but the duration is not limiteddepended on their varieties. The germination analysis of peanut seed isnormally taken for 10 days. If breaking dormant seed is needed, thetime for germination test is totally taken 17 days including preheattime. Therefore, the more rapid way and precise method in order toreduce analysis time would be studied and will be benefit for earlierissue seed certificate.
Ethephon, ethylene liberation, is plant growth regulator that hasbeen reported to break seed dormancy. The comparison betweenpreheat and Ethephon for breaking peanut seed dormancy werestudied in this experiment. Peanut seeds were preheated at 40C for168 h (7 days), and another seed group was directly mixed with 0.96%Ethephon. All pretreated seeds were then planted according tostandard germination test, compared with the no pretreated seed(control). The result found that 0.96% Ethephon was the most benefitto release dormant seed at fresh harvest and achieved 86% emergencewith only 5% fresh seed. The normal seedlings and fresh seeds ofpreheat at 40C for 168 h was 75% and 11%, respectively. Only 6%normal seedling observed in the control and most of seed were stilldormant (89% fresh seeds). After storage at 20C for 28 days, the greatperformances of normal seedlings was observed both Ethephon andpreheat method which showed over 90% normal seedlings and 0-1%fresh seed. While, 42% normal seedlings and 32% fresh seeds werefound in control. The experiment suggests that 0.96%Ethephon is theadvantage method to introduce for breaking dormancy of peanut seedcv. KK 84-7 because it can release dormant peanut seed since freshharvest. Moreover, the duration for germination test is more rapidly(only 10 days) due to preheat for 168 h is not necessary.
Keywords: Peanut seed, Dormancy, Ethephon
Conclusion
Result
0.96% Ethephon is the advantage pretreatment for breaking peanut seed dormancy according to quick and save cost compared to preheat. This method is taken only 10 days including germination test, while preheat at 40C is extended up to 168 h or 7 days before germination test.
Ethephon Effect On Peanut Seed Dormancy Release
Pod seed were stored at 20C for
28 days
Fresh harvested peanut seeds cv.
KK 84-7 were immediately processing as
drying, cleaning, and then carried out to the lab for
experiment.
Sampling randomly
at 0, 7, 14, 21, 28
days after storage
Pretreatment by
Breaking seed
dormancy treatments
were applied.
Germination test by sand method
1. Untreated seed
2. 0.96% Ethephon3. Preheated at
40C for 168 h
cv. KK 84-7
Breaking
seed
dormancy
Control
PlantingSeedling
evaluation
Data record &
Analysis
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0 7 14 21 28
Ge
rmin
atio
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%)
Days after storage at 20C
Control 0.96% Ethephon Preheat at 40°C, 168 h
0
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0 7 14 21 28
Fre
sh s
ee
d (
%)
Days after storage at 20C
Control 0.96% Ethephon Preheat at 40°C, 168 h
Normal seedling
Fresh seed
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a
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a a
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