Estimation of Carbohydrates by anthrone method
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Estimation of Carbohydrate by Anthrone Method • The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides.
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Text Version is Available at: http://www.biochemden.in/2014/11/anthrone-method-carbohydrate-determination.html Carbohydrates are very important component of Storage and structural materials in the plants. The carbohydrates are stored as free sugars and polysaccharides. The basic units of carbohydrates are Monosaccharides. When hydrolyse the carbohydrates, gives monosaccharides, but when hydrolyse monosaccharides it can not be split into more simpler sugars. The hydrolysed product of Polysaccharide are estimating by the resultant monosaccharides.
Transcript of Estimation of Carbohydrates by anthrone method
Estimation of Carbohydrate by
Anthrone Method
• The anthrone reaction is the basis of a rapid
and convenient method for the determination
of carbohydrates, either free or present in
polysaccharides.
* Principle
H2SO4
+
Furfural Anthrone
Blue Green
Complex
Materials
1. Anthrone reagent (0.2% in conc. H2SO4 ).
2. Glucose (10mg/100ml).
3. Colorimeter or spectrophotometer.
Glucose (ml) 0 0.5 0.5
Tube # Blank St. unk.
D. W. (ml) 1 0.5 0.5
Anthrone (ml) 4 4 4
60
30
1545
510
202535
40
5055
COOL to Room TemperatureMix Well
10 min.
Read the Optical Density at 620 nm
Boiling Water Bath
* Calculations
Absorbance of Unknown Absorbance of Standard
=
Concentration of Unknown Concentration of Standard
CUn. = A Un. × C St.
A St.
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