Cold oxygen plasma technology efficiency against airborne respiratory viruses

Terrier O.1, Colin S. 1, VanMechelen D.2, Canu L. 2, Kurtz P. 2 , Ferraris O.1, Lina B.1 and Moules V.1

1VirPath Laboratoire de Virologie et Pathologie Humaine, CNRS FRE 3011, Université Claude Bernard Lyon 1, Faculté de Médecine Laënnec, 69372 Lyon France

2Biozone Europe, France

Cold oxygen plasma technology efficiency against airborne respiratory viruses

Terrier O.1, Colin S. 1, VanMechelen D.2, Canu L. 2, Kurtz P. 2 , Ferraris O.1, Lina B.1 and Moules V.1

1VirPath Laboratoire de Virologie et Pathologie Humaine, CNRS FRE 3011, Université Claude Bernard Lyon 1, Faculté de Médecine Laënnec, 69372 Lyon France

2Biozone Europe, France

What is plasma?

Plasma as a sterilizing agent (Menashi, 1968)

Oxygen plasma efficiency on B. subtilis and Clostridium sporogenes (Nelson and Berger, 1989)

Fourth state of matter

Plasma = ionized gas

Man-made plasma: many ways to make plasma

“Cold” plasma

Energy by radio-frequency, microwave or UV-light…

BiozoneTM technology

Cutting edge air & surface purification technology

Combination of : - Deep ultraviolet light,

- Photo-catalytic oxidation

- Cold oxygen plasma (COP)

Photo-Catalytic oxidation process destroys air pollutants on surfaces

COP eliminates reproductive capability of micro-organisms

Deep UV light sterilizes micro-organisms that pass through the machine

Negative ions decrease total concentration of harmful chemicals in the air

Ozone produced, obliterates bacteria and fungi and remove odour from the air

Airborne respiratory viruses

RSV and hPIV-3

Most important pathogens encountered in low respiratory tract infections in infants and small children (bronchiolitis and pneumonia)

Common reinfections among children and adults (Upper respiratory tract infection).

Infection transmitted by contact with infectious material and by aerosol (droplets)

Influenza

Responsible of recurrent annual epidemics causing acute febrile respiratory illness

Emerging of avian Influenza H5N1: potential threat for a pandemic

Mild to severe illness, all age group

Infection by aerosol (droplets)

Objective

Quantify the impact of deep ultraviolet light and cold oxygen plasma (COP) technologies on viral decontamination of several respiratory viruses

Classic germicide UV-light lamp

BioZoneTM UV-light Lamp (Cold 02 plasma + germicide UV)

External BioZoneTM UV-light Lamp (Cold 02 plasma only)

Human respiratory syncytial virus (RSV)

Human parainfluenza type 3 virus (hPIV-3)

Avian Influenza A/H5N2

Device

Study Design

BSL3 Lab

Viral production Purification/concentration

Viral titration (TCID50/mL)

RSV-A Long strain (ATCC VR-26)

hPIV-3 C-243 strain (ATCC VR-93)

H5N2 A/Finch/England/2051/91 strain

Upstream outlet

Downstream outlet

Nebullization Droplets

Air flow

Flow velocity 0,9m/s Reaction Time 0,44s

UV/COP Device

Results/1

1,00E+00

1,00E+01

1,00E+02

1,00E+03

1,00E+04

1,00E+05

1,00E+06

1,00E+07

1,00E+08

1,00E+09

1,00E+10 InitialUpstream

outlet

Downstream outlet

BioZoneTM

COP onlyClassic UV

No treatment

Impact of UV and cold oxygen plasma on viral decontamination of hPIV-3

TICD50/mL

Comparable effects of UV and COP (4 and 3,6 log destruction rate)

Combination of UV and COP (BioZoneTM Lamp) allow high destruction of hPIV-3: 7 log destruction rate, >99,99% in 0,44s

7 log

BioZoneTM

COP and UV

Results/2

1,00E+00

1,00E+01

1,00E+02

1,00E+03

1,00E+04

1,00E+05

1,00E+06

1,00E+07

1,00E+08

1,00E+09 InitialUpstream

outlet

Downstream outlet

No treatment

Impact of UV and cold oxygen plasma on viral decontamination of RSV

TICD50/mL

Comparable effects of UV and COP (2,1 and 2 log destruction rate)

Combination of UV and COP (BioZoneTM lamp) allow high destruction of RSV: 3,8 log destruction rate, >99,99% in 0,44s

3,8 logClassic

UVBioZoneTM

COP only

BioZoneTM

COP and UV

1,00E+00

1,00E+01

1,00E+02

1,00E+03

1,00E+04

1,00E+05

1,00E+06

1,00E+07

1,00E+08

1,00E+09

Results/3Impact of UV and cold oxygen plasma on viral decontamination of H5N2

Initial

Upstream outlet

Downstream outlet

No treatment

Classic UV

BioZoneTM

COP and UV

BioZoneTM

COP only

Comparable effects of UV and COP (3,5 and 2,9 log destruction rate)

Combination of UV and COP (BioZoneTM lamp) allow high destruction of H5N2: 5,5 log destruction rate, >99,99% in 0,44s

5,5 log

TICD50/mL

Conclusions and Perspectives

Difference of results between viruses could be explain by virus properties (structure of viral envelope) or viral suspension preparation.

High efficiency of BiozoneTM lamp in viral decontamination

Future tests with other viruses (rhinovirus, hepatitis virus…) Surface decontamination, Photocatalytic oxidation parameter

Cold oxygen plasma technology could be an innovatory and efficient way to decontaminate air to protect human populations against respiratory viruses

=> Decontamination of hospital wards, BSL3 labs…

Acknowledgements

We thank all the Staff at Centre de biologie et de Pathologie Est clinical virology lab (Lyon, France) and all the staff of VirPath lab (Bruno Lina’s group, Lyon, France)