مقاله پژوهشي

1116

Escherichiaيها زولهيادر CTX-M-1 ژن يفراوان

coli نمونه در ف يالط عيم بتاالکتاماز وسيمولد آنز

اي زنجيره واکنش روش با ينيبالهاي

در ياسوج PCRپليمراز

*1يد خسروانيد عبدالمجيس 3اييسيده مرضيه طباطب1يفيصغر شر1د سجادخرم روزيس 2ينيحس يمهر ،1شونديزهرا خو

ده يچک

هدف و

. (ESBLs) E. coli

CTX-M-1Escherichia coli

PCR

روش بررسي

CLSI

.CTX-M1ESBLPCR

. CTX-M-1 E.coli PCR ها: افتهي

PCR

CTX-M-1

.

E.coli يريگ جهينت

E. coli CTX-M-1کليدي: يها هواژ

،يد خسروانيد عبدالمجيس نويسنده مسئول:*Email: khosravani2us@yahoo.com

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1117

مقدمه

.

E. coli

Escherichia coli

.

Beta-lactamase-enzymes

Extended

Spectrum Bete-Lactamase

ABCD

A

TEMSHV CTX-M

ESBL

SHV TEM

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1118

ESBLs

CTX-M

)CTX-M-9 CTX-M-8 CTX-M-25CTX-M-2 (CTX-M-1

.E. coli

ESBL

E. coli

.

.

CTX-M

ESBL

.

E.coli

CTX-M-1PCR

يروش بررس

E. coli

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1119

EMB

MRVPSIMTSI

Escherichia coli

E. coli

CLSI (Clinical & Laboratory Standards Institute) M100-

A20 (2010)

CTX-M-1

DNA boiling

PCR

SPSS

ها يافته

E. coli

.

.

Combined disk

E. coli

.

CTX-M-1

E. coliESBLs

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1120

CTXM-1ژن يمرهايپرا: 1جدول

850bp F: 5GGTTAAAAAATCACTGCGTCʹ 3 R: 5TTGGTGACGATTTTAGCCGCʹ 3

CTXM-1

تر شود ح شکل کامليتوض CTX-M-1: يژن مورد بررس PCRجه ي: نت2شکل

تر شود ح شکل کامليتوض مثبتESBLs مولدين فنوتيپي تشخيص: 1شکل

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1121

زوله ها. يا يکيوتيب يدرصد مقاومت آنتنمودار : 1نمودار

بحث

Enterobacteriaceae

Enterobacteriaceae ESBL

CTX-M-1PCR

ESBL

SHVTEM CTX-M

blaCTX-M

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1122

.

ESBL

CTX-M-1

CTX-M

E. coli

ESBLs

CTX-M-1

E.coli

MIC

PCR blaCTX-M

blaSHV blaTEM

CTX-M

E. coli

DADPCR ESBL

TEM

160 E.coli

CTX-M PCR

CTX-M-1 CTX-M-5

CTX

CTX-M

SHVTEM

CTX-M-3

PCR

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يريگ جهينت

ESBL

ESBL

تقدير و تشکر

IR.YUMS.REC.139494

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19.Shahcheraghi F, Nasiri S, Noveiri H. The survey of genes encoding beta-lactamases, in escherichia coli resistant to beta-lactam and non-beta-lactam antibiotics. Iranian Journal of Basic Medical Sciences 2007; 13(1): 230-7 20.Dallal M, Sabbaghi A, Fallah J, Aghamirzaei HM, Lari A, Eshraghian MR, et al. Evaluation of presence of the bla-SHV and bla-AmpC (CITM, FOX) β-lactamase genes in clinical isolates of Escherichia coli. Journal of Medical Council of Islamic Republic of Iran 2010; 28(3): Pe269-76. 21.Ho PL, Wong R, Chow KH, Yip K, Wong S, Que TL. CTX-M type beta-lactamases among fecal Escherichia coli and Klebsiella pneumoniae isolates in non-hospitalized children and adults. Journal of Microbiology 2008; 41(5): 428-32 . 22.Jonas Bonnedahl , Mirva Drobni , Michel Gauthier-Clerc, Jorge Hernandez, Susanne Granholm, Yves Kayser, Åsa Melhus, Gunnar Kahlmeter, Jonas Waldenström, Anders Johansson, Björn Olsen. Dissemination of Escherichia coli with CTX-M type ESBL between humans and yellow-legged gulls in the south of France. PloS One 2009; 4(6): 23.Shahcheraghi F, Noveiri H, Nasiri S. Detection of bla TEM and bla SHVgenes among clinical isolates of E. coli from Tehran hospitals. Iranian Journal of Medical Microbiology 2007; 1(3): 1-8. 24.Mirzaee M, Pourmand MR, Chitsaz M, Mansouri S. Antibiotic resistance to third generation cephalosporins due to CTX-M-Type extended-spectrum β-lactamases in clinical isolates of Escherichia coli. Iranian Journal of Public Health 2009; 38(1):10-17

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Armaghane-danesh, Yasuj University of Original Article

Medical Sciences Journal (YUMSJ)

Frequency of CTX-M-1 Gene in

Escherichia coli Isolates of

ESBL-Producing Enzyme in Clinical

Samples by Polymerase Chain Reaction

(PCR) in Yasuj

Khishvand Z1, Hosseini M

2, Khoram Rooz SS

1, Sharifi A

1, Tabatabaei SM

3, Khosravani SAM

1*

1Cellular and Molecular Research Center, yasuj University of Medical Sciences, yasuj, Iran,

2Student

Research Committee, yasuj University of Medical Sciences, yasuj, Iran, 3Department of Genycology, Yasuj

University of Medical Sciences, Yasuj, Iran

Received: 03 Feb 2019 Accepted: 03 Des 2018

Abstract Background & aim: Resistance to β-lactam antibiotics in the clinical isolates, in most cases is caused by β-

lactamase enzymes. In recent years, The incidence of broad-spectrum β-lactamase enzymes (ESBLs) among clinical isolates especially E.coli is greatly increased, since the β-lactamase have several subfamilies, using universal primers designed to detect the following complete families could be useful. β-lactamase producing enzymes (ESBLs) of E. coli has created many problems for patients. β-lactamase CTX-M-1 gene is the cause of resistance. The aim of this study was to evaluate CTX-M-1gene in E.coli. Methods: In this practical study, susceptibility of isolated bacteria to 13 antibiotics were indicated by disk

diffusion method according to CLSI guidelines and strains were analyzed for the presence of widespread β-lactamase enzymes via two-disc synergy method. Thus، the prevalence of CTX-M1 ESBL gene samples were determined using PCR and the data were analyzed using ANOVA. Results: A total of 200 isolates of E.coli were isolated. The presence of CTX-M-1 gene were also isolated

using the PCR method. From 200 strains studied, 62 (31%), of strains produced ESBL. After PCR processing of 62 produced ESBL, 43 isolates (69.4%) were identified as CTX-M-1 genes. Also, antibiotic susceptibility test showed the highest percentage of resistance to Cotrimoxazole antibiotic (50%) and the lowest antibiotic resistance to imipenem (0%). Conclusion: The results of this study showed the high percentage of β-lactamase resistance among of E.coli

strains. This is a serious public hazard that should be pointed out to measures for preventing this hazard. Considering the sensitivity of the studied beta-lactam resistant isolates and isolates in Iran to imipenem, a carbapenem with a non-beta-lactam antibiotic is recommended for the treatment of nosocomial infections caused by these strains. Keywords: E.coli, ESBL, PCR, CTXM1

*Corresponding author: Khosravani SAM, Cellular and Molecular Research Center, yasuj University of

Medical Sciences, yasuj, Iran Email: khosravani2us@yahoo.com Please cite this article as follows: Khishvand Z, Hosseini M, Khoram Rooz SS, Sharifi A, Tabatabaei SM, Khosravani SAM. Frequency of CTX-M-1 Gene in Escherichia Coli Isolates of ESBL-Producing Enzyme in Clinical Samples by Polymerase Chain Reaction (PCR) in Yasuj. Armaghane-danesh 2020; 24(6): 1116-1126.

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