Epstein-Barr Virus · Epstein–Barr virus (EBV) is one of the most common viruses in humans. The...

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LAB Epstein-Barr Virus Lyophilized Real-Time PCR mix for the detection and quantification of EBV

Transcript of Epstein-Barr Virus · Epstein–Barr virus (EBV) is one of the most common viruses in humans. The...

Page 1: Epstein-Barr Virus · Epstein–Barr virus (EBV) is one of the most common viruses in humans. The virus lives in a person’s salivary glands and can be spread through saliva, such

LAB Epstein-Barr VirusLyophilized Real-Time PCR mix for the detection and quantification of EBV

Page 2: Epstein-Barr Virus · Epstein–Barr virus (EBV) is one of the most common viruses in humans. The virus lives in a person’s salivary glands and can be spread through saliva, such

LAB

Lyophilized Real-Time PCR mix for the detection and quantification of EBV

Epstein–Barr virus (EBV) is one of the most common viruses in humans. The virus lives in a person’s salivary glands and can be spread through saliva, such as by kissing or sharing drinking glasses or eating utensils. About half of all five-year-old children and almost 90% of adults have evidence of previous EBV contagion. EBV infection is best known as the cause of infectious mononucleosis, but it is also associated with particular forms of cancer, a higher risk of certain autoimmune diseases and transplants complications.

STAT-NAT® EBV kit proved its effectiveness for the detection and quantification of EBV DNA in samples. The high-sensitivity and specificity, linearity and quantitation performances of STAT-NAT® EBV kit, associated with the ready-to-use and room temperature storage, are key features for the early and correct management of the affected patients.

Standard Curve (104, 103, 102 and 10 EBV copies/reaction)

Benefits of the kit:• Detection and Quantification of Epstein-Barr Virus (EBV)

specific DNA• Ready to use kit including internal Control and

Quantification Standards• Large range of samples: whole blood, serum, plasma,

CSF, saliva• Optimal performances: high sensitivity and specificity

Epstein-Barr Virus

Ref. Description Kit size Validated on

1N011 STAT-NAT® EBV Lyophilized Real-Time PCR mix for the detection and quantification of EBV 48 tests • CFX96 (Bio-Rad)

• QS5 (ABI)

STAT-NAT® EBV quant

Specificity ≥95%

Analytical sensitivity (LoD) 10 cps/reaction

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Patent No. W02010133628 A1

Sentinel CH. SpAVia Robert Koch, 2 � 20152 Milan - Italy Phone: +39 02 3455141 � Fax: +39 02 34551464 [email protected] � www.sentineldiagnostics.com

FlexibleSTAT-NAT® can improve test sensitivity even with low concentrated DNA samples.

UniversalSTAT-NAT® technology yields very good performances in all the most diffused molecular biology techniques.

Environmentally sustainableSTAT-NAT® needs neither cumbersome packaging nor temperature controlled shipment.

Room temperature storageSTAT-NAT® is freeze-dried and guarantees long term storage at room temperature.

EasySTAT-NAT® contains all the reaction components. The enzyme (Hot Start Polymerase) is already included.

Performance improvementSTAT-NAT® is a ready-to-use product that minimizes analytical variables.

Molecular Biology made easy!

Amplification

EBV

RFU

Internal control

Cycles0 5 10 15 20 25 30 35

8000

7000

6000

5000

4000

3000

2000

1000

0

Standard Curve

Cq

Log Starting Quantity

FAM E=96,8% R^2=0,999 Slope=-3,400 y-int=30,076

Standard

1,0 1,5 2,0 2,5 3,0 3,5 4,0

26

24

22

20

18

16

Availablefrom

June 2019