Epstein-Barr Virus · Epstein–Barr virus (EBV) is one of the most common viruses in humans. The...
Transcript of Epstein-Barr Virus · Epstein–Barr virus (EBV) is one of the most common viruses in humans. The...
LAB Epstein-Barr VirusLyophilized Real-Time PCR mix for the detection and quantification of EBV
LAB
Lyophilized Real-Time PCR mix for the detection and quantification of EBV
Epstein–Barr virus (EBV) is one of the most common viruses in humans. The virus lives in a person’s salivary glands and can be spread through saliva, such as by kissing or sharing drinking glasses or eating utensils. About half of all five-year-old children and almost 90% of adults have evidence of previous EBV contagion. EBV infection is best known as the cause of infectious mononucleosis, but it is also associated with particular forms of cancer, a higher risk of certain autoimmune diseases and transplants complications.
STAT-NAT® EBV kit proved its effectiveness for the detection and quantification of EBV DNA in samples. The high-sensitivity and specificity, linearity and quantitation performances of STAT-NAT® EBV kit, associated with the ready-to-use and room temperature storage, are key features for the early and correct management of the affected patients.
Standard Curve (104, 103, 102 and 10 EBV copies/reaction)
Benefits of the kit:• Detection and Quantification of Epstein-Barr Virus (EBV)
specific DNA• Ready to use kit including internal Control and
Quantification Standards• Large range of samples: whole blood, serum, plasma,
CSF, saliva• Optimal performances: high sensitivity and specificity
Epstein-Barr Virus
Ref. Description Kit size Validated on
1N011 STAT-NAT® EBV Lyophilized Real-Time PCR mix for the detection and quantification of EBV 48 tests • CFX96 (Bio-Rad)
• QS5 (ABI)
STAT-NAT® EBV quant
Specificity ≥95%
Analytical sensitivity (LoD) 10 cps/reaction
DPL
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Patent No. W02010133628 A1
Sentinel CH. SpAVia Robert Koch, 2 � 20152 Milan - Italy Phone: +39 02 3455141 � Fax: +39 02 34551464 [email protected] � www.sentineldiagnostics.com
FlexibleSTAT-NAT® can improve test sensitivity even with low concentrated DNA samples.
UniversalSTAT-NAT® technology yields very good performances in all the most diffused molecular biology techniques.
Environmentally sustainableSTAT-NAT® needs neither cumbersome packaging nor temperature controlled shipment.
Room temperature storageSTAT-NAT® is freeze-dried and guarantees long term storage at room temperature.
EasySTAT-NAT® contains all the reaction components. The enzyme (Hot Start Polymerase) is already included.
Performance improvementSTAT-NAT® is a ready-to-use product that minimizes analytical variables.
Molecular Biology made easy!
Amplification
EBV
RFU
Internal control
Cycles0 5 10 15 20 25 30 35
8000
7000
6000
5000
4000
3000
2000
1000
0
Standard Curve
Cq
Log Starting Quantity
FAM E=96,8% R^2=0,999 Slope=-3,400 y-int=30,076
Standard
1,0 1,5 2,0 2,5 3,0 3,5 4,0
26
24
22
20
18
16
Availablefrom
June 2019