Epidemiology and control of Sclerotinia · Epidemiology and control of Sclerotinia Dr John Clarkson...
Transcript of Epidemiology and control of Sclerotinia · Epidemiology and control of Sclerotinia Dr John Clarkson...
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Warwick Crop Centrewww.warwick.ac.uk/go/wcc
Epidemiology and control of
Sclerotinia
Dr John Clarkson
Warwick Crop Centre, University of Warwick
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Talk Outline
• Sclerotinia sclerotiorum in the UK
• Approaches to control:
• Sclerotia, apothecial production and
biofumigation
• Ascospores, infection and disease
forecasting
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Ascospores
infect carrot
Lifecycle of Sclerotinia sclerotiorum
Carpogenic germination
of sclerotia near soil
surface to produce
apothecia
Apothecia release
ascospores
Sclerotia survive
in soil over winter
Further sclerotia
form and released
into soil
> 400 host species
including: oilseed rape, lettuce,
carrot, potatoes, beans, peas,
sunflower, celery…etc
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Sclerotinia disease in the UK
• Caused by Sclerotinia sclerotiorum• Little S. minor
• Some S. subarctica in Scotland
• Significant losses in all susceptible crops
• Incidence is increasing year on year
• Shortened rotations
• Problem in timing fungicides
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Oilseed Rape (600,000 ha)
• Occasional outbreaks with 50-80% loss
• Average 18% of crops affected each year
• 10% incidence = loss of 0.2t / ha
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Lettuce (6000 ha)
• Typical losses are 5-10% each year but can be up to 50%
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Carrot (10,000 ha)
• Losses up to 50%
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Wild hostsRanunculus (buttercup)Cirsium (thistle)
Urtica (nettle) Chenopodium
(fat hen)
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The ‘bank’ of sclerotia
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Number of sclerotia produced per plant
0
20
40
60
80
100
120
140
160
Lettuce Bean Carrot Potato OSR
Scle
roti
a p
rod
uce
d p
er
pla
nt
L44
L17
L6
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Weight per sclerotium
0
0.02
0.04
0.06
0.08
0.1
0.12
Lettuce Bean Carrot Potato OSR
We
igh
t o
f in
div
idu
al s
cle
roti
um
(g) L44
L17
L6
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Number of sclerotia produced per square metre
0
500
1000
1500
2000
2500
3000
3500
Lettuce Bean Carrot Potato OSR
Nu
mb
er
of
sc
lero
tia
pro
du
ce
d p
er
sq
ua
re m
etr
e
673
427
3035
1435
188
Crop density (plants m-2): Lettuce = 8 , Bean = 40 , Carrot = 150, Potato = 3, OSR = 28
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Effect of sclerotial size on germination
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Approaches for Sclerotinia control
• Reduce soil inoculum (sclerotia)• Perlka – calcium cyanamide
• Biocontrol – Contans
• Biofumigation
• Kill ascospores – improve fungicide timing by forecasting
• Plant resistance• Tolerant varieties only?
• Breeding efforts focussed on brassica
• Warwick project on lettuce resistance
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Biofumigation
• Biofumigants e.g. mustards
• Growth and / or incorporation stimulates beneficial microorganisms
• Glucosinolates converted to fungitoxic isothiocyanates (ITCs)
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ITCs inhibit mycelial growth of S. sclerotiorum
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Volatiles directly affect germination of sclerotia
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Effect of biofumigants on germination of S.
sclerotiorum sclerotia (microcosms)
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S. sclerotiorum polytunnel experiment
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Forecasting Sclerotinia
• How can we assess disease risk ?
1) Direct detection of airborne ascospores
• PCR test has now been developed (Rothamsted)
2) Monitoring / predicting production of apothecia
• model developed which predicts germination of sclerotia using weather data.
3) Predicting infection
• Infection / disease development dependent on RH / temperature.
4) Crop growth stage models
• Senescent material important in Sclerotinia disease development
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Spore trap monitoring by PCR in oilseed rape
(Rothamsted)
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Monitoring sclerotial germination
But when to bury
sclerotia ?
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Two processes control germination of sclerotia
Production of apothecia by germinating sclerotia involves two processes:
1) ConditioningChilling required for rapid production of apothecia (optimum <7C)Requires soil moisture ( > - 100 kPa)Varying response between isolates
2) GerminationTemperature dependent (optimum 15-18C) Requires soil moisture ( > - 100 kPa)
We produced a prediction model based on soil temperature and moisture.
Defra funded project in collaboration with Caroline Young, ADAS. Model published: Clarkson et al., Phytopathology 2007.
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Germination of sclerotia in the field driven by temperature / moisture
0
10
20
30
40
50
60
70
80
90
100
Mar Apr May Jun Jul Aug Sep Oct Nov
% g
erm
ina
tio
n
winter 23/12
burial 1 04/3
burial 3 31/3
burial 5 28/4
burial 6 12/5
burial 7 27/5
burial 8 10/6
0
10
20
30
40
Mar Apr May Jun Jul Aug Sep Oct Nov
Te
mp
soil temp
soil moisture
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Model Prediction and observed germination
0
5
10
15
20
25
30
35
40
45
50
16/10 30/10 13/11 27/11 11/12 25/12 08/01 22/01 05/02 19/02 04/03 18/03 01/04 15/04 29/04 13/05 27/05 10/06 24/06
Date
Tem
pera
ture
(°C
) /
Rain
fall
(m
m)
0.00
0.20
0.40
0.60
0.80
1.00
1.20
Mo
del
Pro
gre
ss
Soil temperature
Rainfall
Predicted germination progress
Observed germination
Predicted
germination
25th April
Observed
germination
2nd May
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Spray timing and Sclerotinia infection in lettuce
0
2
4
6
8
10
12
14
untreated 1 spray 2 spray 3 spray model (1 spray)
% p
lan
ts w
ith
Scle
roti
nia
G's
Merrymacs
Site 1
Site 2
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The Future: regional prediction of sclerotial germination
BASF PhD
• Further development:
• Improve prediction of soil moisture from
rainfall for the model
• Understand what happens to sclerotia buried
at different depths
• Determine any effect of isolate variation
• Envisage network of weather stations
combined with direct ‘depot’ observations
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Predicting infection by ascospores
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Predicting infection: humidity and disease
development
0
10
20
30
40
50
60
70
80
90
100
0 5 10 15 20 25 30 35 40 45 50 55 60 65
days
% p
lan
ts in
fec
ted
wit
h S
cle
roti
nia
100% RH
90% RH
80%RH
70%RH
60%RH
50%RH
Disease development on lettuce at 20C
• Sclerotinia infects lettuce in the
absence of free water as
microsites around stem base
are still conducive to infection.
• BUT ambient RH modifies no.
of effective microsites and rate
of disease development
• Reducing RH in crop canopies
will reduce the number of
infections
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Clipping carrot foliage
Source: Root Crop Consultancy Limited with support from BASF
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Predicting infection: temperature and disease
development
0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50 60 70 80 90 100 110 120
% p
lan
ts in
fec
ted
wit
h S
cle
roti
nia
days
25C
20C
15C
10C
7C
Disease development at 85%RH
Sklero Pro model developed for oilseed rape
(Koch et al., 2007)
23 continuous hours required where
RH > 80%
Air temperature > 7C
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Combined approach to Sclerotinia prediction in
oilseed rape (ADAS)
• Inputs
• Crop growth stage (flowering)
• Sklero Pro infection criteria met
• Petal testing (by plating or PCR)
• Presence of airborne inoculum
(Sklero Pro)
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Population Structure and Diversity
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Why study diversity and population structure?
• No previous studies in the UK or on wild hosts.
• Is diversity related to biological variation in• isolate aggressiveness
• ability of sclerotia to germinate and produce apothecia
• Are certain Sclerotinia genotypes adapted to particular hosts?
• Is there genotype exchange between wild and agricultural hosts?
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carrot celery pea
lettuce oilseed rape buttercup
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S. sclerotiorum microsatellite genotypes
• Sclerotinia is diverse: 386 genotypes from 687 isolates (multiple isolates just in one field)
• Genotypes are shared between crop plants and / or wild hosts: prevalent: 56 genotypes shared between one or more population, 29 genotypes shared between crop plants and buttercup.
• One genotype is common and widespread:prevalent genotype comprises 10% of isolates and is found year after year at different locations
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Back to biology: pathogenicity variation for 18
S. sclerotiorum isolates from crop plants and buttercup
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Acknowledgements
Project funding: Defra, HDC
UK Collaboration: Caroline Young (ADAS), Jon West
(Rothamsted)
Norway Collaboration: Berit Nordskog and Andrea Ficke
Helen Carter Xinyang Hong Emma CoventryAngela Foster Rachel
Warmington
Andrew Taylor
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Thank you
for listening
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Cavity spot on carrot
• The major disease of carrot in the UK:
losses of £3-5 million per season
• Caused primarily by the oomycete P.
violae, in the UK and to a lesser extent P.
sulcatum
• Investigating biology of Pythium spp.
involved, artificial inoculation techniques
and further developing specific PCR test
(PhD).
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Fusarium on onion
• Caused by Fusarium oxysporum f. sp.
cepae (FOC)
•One of the biggest problems for UK onion
growers and set producers
• Infects roots and basal plate
• Fundamental research investigating genetic
basis for pathogenicity and development of
resistant onion lines with Hazera Seeds
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European Innovation Partnership 'Agricultural
Productivity and Sustainability'
• Focus group: IPM practices for soil-borne diseases
suppression in vegetables and arable crops
• http://ec.europa.eu/eip/agriculture/en/content/ipm-practices-
soil-borne-diseases-suppression-vegetables-and-arable-crops