Enzymes Notes: Part II. Enzymes and their Environment All enzymes have an ideal environment that...
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Transcript of Enzymes Notes: Part II. Enzymes and their Environment All enzymes have an ideal environment that...
Effects on EnzymesEnzymes Notes: Part II
Enzymes and their EnvironmentAll enzymes have an ideal environment that they function in
Think of them as organisms adapted to their specific environment and nicheWho would survive better in Antarctica, a polar bear or a camel?What enzyme would be more effective in an acidic environment,
a protease in your stomach or amylase in your mouth?
What effects enzymes?TemperaturepHEnzyme/Substrate Concentration
DenaturingWhen an enzyme loses its structure and becomes non-
functional due to drastic changes in temperature or pH
TemperatureEvery enzyme has an ideal temperature
range that it functions inOutside of this range, the enzyme may
become denatured
Enzymes in the Human Body
pHWhat is pH?
Measure of acidic or basic a solution is
pH and EnzymesEvery enzyme has an ideal pH range it is
used to working in based on its environmentOutside of this range, it can become
denatured
Enzymes in the Human Body
pH ScaleLet’s learn more about the pH Scale!
http://www.brainpop.com/science/matterandchemistry/phscale/
ConcentrationHow much stuff (solute) is dissolved in a
certain amount of solution (solvent)How much enzyme and substrate there are
in a solution can effect how productive the enzyme is
ConcentrationIf enzyme concentration is kept constant,
and substrate levels are increased, then, the reaction rate will increase until it reaches its maximum level
Enzyme Denaturation Review!http://www.youtube.com/watch?v=3IL_Df5ou
Uc
http://www.kscience.co.uk/animations/model.swf
Enzyme Concentration Review!
Do NowIdentify the BEST pH for the following three
enzymes and identify what pH ranges they would become denatured.
Example Questions!Which of the following most accurately
represents the effects of temperature on enzyme reactions in humans?
Example Questions!Which of the
following most accurately represents the effects of temperature on enzyme reactions in a thermophilic bacteria?
Example Questions!The reaction catalyzed by the bacterial enzyme ß-galactosidase forms a dark-
colored end-product when the cells are grown on a particular agar medium. As more product is formed, the cells become darker. Students performed an experiment to determine the optimum pH for activity of this enzyme. Their results are shown in the illustration of bacterial colonies below.
Based on these data, the students should conclude that ß-galactosidase functions best at which pH?A. 5 B. 7 C. 9 D. 11
Example Questions!All of the following statements are true
about enzymes EXCEPT:A. Enzymes are specific to one substrate and
reactionB. Once enzymes are used once, they can be
used over and over againC. Enzymes raise the activation energy of a
reactionD. Enzymes speed up the rate of reactionsE. Temperature and pH changes can cause
enzymes to lose their function