Environmental Analysis Tips withwith

EnviroQuant Software

John PellerinGC/MS Technical SpecialistGC/MS Technical Specialist February 11, 2009

Slide 1

Software Revision Used

Slide 2

Topics

• Easy ID

• Mixed Curve Fit ReportsMixed Curve Fit Reports

• Normalized TIC Overlays

El t i PDF R t• Electronic PDF Reports

• Custom Report Database

Slide 3

Easy ID Updates Method Parameters

• Set Retention Times

• Set Signal Extraction Windows

• Manually Update Reference Spectra

• Determine Resolution of Two Peaks

Slide 4

Changed Retention Time for Demonstration

Slide 5

Easy ID ToolBar

Slide 6

Easy ID Menu Item

Slide 7

Mouse Actions

Shift Double Click Left Mouse Button for Start Extraction Time Window

Shift Double Click Right Mouse for End Extraction Time Window

Click and Drag Right Mouse Button for Manual Integration to Obtain Peak Retention Time

Slide 8

Set Extraction Window and Retention Time

Slide 9

New Values

Slide 10

Update Method

Slide 11

Method Updated

Slide 12

Display Reference Spectra

Slide 13

Incorrect Reference Spectrum

Slide 14

QREF Updates Reference Spectra from Data File

Slide 15

Reference Spectra Stored in Methods Folder

Slide 16

Reference Spectra Set Under Reporting

Slide 17

Library Entries Can Be Used For Ref. Spectra

Slide 18

Example Library Reference Spectra

Slide 19

SAV6 is One Spectrum from a Data File

Slide 20

Obtain Correct Spectrum and Update

Slide 21

New Reference Spectrum Saved to SAV6

Slide 22

Resolution Calculation

Slide 23

Manually Integrate Each Peak

Slide 24

Resolution Calculated

Slide 25

Multi Curve Fit Calibration Report

• Enhance 2 Command Toggles to Enhance Mode

Enhance 1 Command Toggles to EnviroQuant Mode• Enhance 1 Command Toggles to EnviroQuant Mode

• Slog 1 Logs Code Executed

• Endlog Displays Logfile

• Hammers Used for Customization

Slide 26

EnviroQuant Menu

Slide 27

Enhanced 2 Toggled to Enhanced Menu

Slide 28

Under Calibrate Select List

Slide 29

Select Calibration Report

Slide 30

Multi Curve Fit Calibration

Slide 31

Slog 1

Slide 32

Endlog

Slide 33

Enh_callist Executes Dialogue Box

Slide 34

Enh_callist

Slide 35

wwcal Generates Report

Slide 36

Report Generated

Slide 37

Enhance 1 Toggle Back to EnviroQuant

Slide 38

Custom Tool (Hammer)

Slide 39

Custom Tool1 Undefined

Slide 40

Replace Lines

Slide 41

Hammer 1 Generates Multi Curve Fit Report

Slide 42

Normalize Chromatograms

• Clwv020 (x 10)

• Clwv050 (x 4)

• Clwv100 (x 2)

• Clwv150 (x 1.33)

• Clwv200Clwv200

Slide 43

Clwv20 is Loaded

Slide 44

Select TIC

Slide 45

Select Files

Slide 46

Files Overlaid

Slide 47

Internal Standards Same Amount

Slide 48

Zoomed IS

Slide 49

Shift-Alt Right Mouse Double Click Toggle

Slide 50

(Select TIC) CTRL-ALT Right Mouse Double Click

Slide 51

Perform for Each TIC

Slide 52

Normalized Chromatogram

Slide 53

Zoomed IS

Slide 54

PDF File Generation

Need to Purchase Adobe Acrobat Standard/Professional

Slide 55

Adobe Acrobat Installed Print Driver

Slide 56

Interactive PDF Generation

Slide 57

Name File and Select Location

Slide 58

View PDF File

Slide 59

Automatic Generation of PDFs

Slide 60

PDFWIZ.mac

Slide 61

Msdchem.ini

Slide 62

Tune Files (versioned)

Slide 63

Tune Files

Slide 64

PDF Tune File

Slide 65

Run Method Printer

Slide 66

Report Versioned

Slide 67

PDF Quant Report

Slide 68

PDF Quant Report

Slide 69

PDF Quant Report

Slide 70

Custom Report Database

MDL Example

Slide 71

Start Custom Reports

Slide 72

Create New Database

Slide 73

Add Amount for All Compounds

Slide 74

Select Data File to Populate Database

Slide 75

Save Database

Slide 76

Data Entered

Slide 77

Select Compounds

Slide 78

Paste into Excel

Slide 79

Select Amounts

Slide 80

Paste into Excel

Slide 81

Copy Compounds and Amounts to Sheet 2

Slide 82

Use Paste Special to Paste with Transpose

Slide 83

Data Transposed in Sheet2

Slide 84

MDL Report

Slide 85

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