Engineering BiologyChee 450

Prof. Ron NeufeldDupuis 230, 533-2785

neufeld@chee.queensu.ca

Acknowledgments

Many images in this course originate from the web. The source maybe determined by placing your cursor over the image. In a few cases,images were scanned from textbooks or literature journals. The sourceof any image may be obtained by contacting neufeld@chee.queensu.ca.A few of the images actually belong to the instructor.

Instructor before hebusted his electricshaver

Office hours and location

• 7:48 am to 5:39 pm, Monday to Friday if I am there

• e-mail any time – response usually same day

• office over front “smoking” entrance of Dupuis Hall

• lab office Biosciences 1443

Course web page

• reminders• course outlines (and notes)• assignments (and solutions)• test and exam dates

Course objectives

• Biochemical Engineering is the application of Chemical Engineering principles and approaches to biologically-based systems and processes

• Biological Engineering will examine the underlying biological and biochemical principles

Course text

• Zen and the Art of Motorcycle Maintenance, by Ronald DiSanto

Reference material

• Shuler and Kargi, Bioprocess Engineering: Basic Concepts

• Blanch and Clark, Biochemical Engineering

• Bailey and Ollis, Biochemical Engineering Fundamentals

More reference material

• Palsson, Hubbell, Plonsey and Bronzino, Tissue Engineering

• Lanza, Langer and Vacanti, Principles of Tissue Engineering

• Prescott, Harley and Klein, Microbiology (section on Industrial Microbiology)

Search for Stem Cells on:• http://www.eurekalert.org/

EurekAlert is run by the American Association for the Advancement of Sciences.

• http://scitechdaily.com/Range of news articles, features, and commentaries about science and technology

• http://www.sciam.com/index.cfmScientific American website

• http://www.reuters.com/news.jhtml?type=science Reuters news site for stories about science. Search, then select "News and Pictures."

Course evaluation

• mid-term test: 25%

• assignments: 20%

• design project/report: 15%

• final exam: 40%

• contributions of cookies and muffins to class: according to level of class participation

History and overview of the products of Biochemical Engineering

Citric acid AntibioticsBrewing/Bioethanol Amino acids Monoclonal antibodies Therapeutic proteinsFarmaceuticalsSite directed mutagenesis

Principles of Cell Culture

Properties and structure of the cell

NomenclatureBacteriophageGrowth behaviorDetermining cell massChemical composition of cells

Tissue engineering

Tissue culture with mammalian and plant cellsProducts and medical applicationsLarge-scale mammalian cell culture systems

Metabolism and product formation

Metabolic pathways – catabolic/anabolicBiosynthesisStoichiometry and energeticsControl and regulationIndustrial strain improvementScreening for new metabolitesMolecular genetics and control

Enzymes and enzyme kinetics(versus Monod growth kinetics)

Naming and classificationApplications – purification of racemic mixtures of amino acidsReaction kinetics (Michaelis-Menten)InhibitionBatch and continuous reactor kinetics

Immobilized biocatalysts

Immobilization methodsImmobilized enzyme/cell kineticsMass transfer resistance, effectiveness

factors

Product separation and purification

Downstream process scheme in protein purification

Design project:A downstream separation train will be examined in detail and each of you assigned one particular unit operation within the purification protocol for a detailed design. The design will be presented in class, followed by a written report.