ELISA Brochure

Enzyme-Linked ImmunoSorbent Assay

See more with KPL ELISA products —Get higher signal and lower background.

SEE MORE with KPL!

ELISA

Sometimes it is difficult to distinguish your protein of interest

from background. However, with KPL Protein DetectorTM ELISA

products you get strong, clean signal every time. KPL’s ELISA

products offer consistent, high quality results and enable

sensitive immunodetection of protein in a microwell format.

Choose from a broad line of sensitive substrates, high quality

kits and stand-alone reagents for detecting and quantifying specific

antigens in a complex protein mixture. Combine these products

with KPL’s extensive line of secondary antibodies to develop

reproducible and sensitive ELISAs.

• Substrates for colorimetric or chemiluminescent detection

• Secondary antibodies unlabeled or labeled with either

horseradish peroxidase (HRP) or alkaline phosphatase (AP)

• Support reagents – blocking solutions, coating buffers, wash

solutions, conjugate stabilizers, stop solutions

• Universal ELISA kits formatted with HRP- or AP-labeled

secondary antibodies

The foundation of KPL’s Protein Detector ELISA offering is a

broad line of chemiluminescent and chromogenic substrates

for use with HRP and AP conjugates that provide varied levels

of sensitivity. For highly sensitive colorimetric peroxidase detec-

tion, choose SureBlue Reserve™ Microwell Substrate with long

shelf life and convenient 1-component format. New FirePhos™

Microwell Substrate expands our selection of sensitive sub-

strates for phosphatase detection. LumiGLO Reserve™

Chemiluminescent Substrate offers the ultimate in sensitivity

via chemiluminescent reaction with HRP. No matter which you

choose, KPL’s ELISA substrates are all tested to assure lot-to-lot

consistency, reproducibility and long-term stability.

An extensive line of high-quality secondary antibody conjugates and

labeled streptavidin are also available. Each is affinity purified to

assure excellent signal-to-noise results in ELISAs. KPL support

reagents are optimized for use in microwell ELISA and tested

to verify that they meet strict specifications. They include

conjugate stabilizers, diluents, blocking and wash solutions, all

formulated to ensure accurate and reproducible results.

Protein Detector Kits provide a convenient starting point for

the development of ELISA protocols. Each kit contains

enzyme-labeled secondary antibody, corresponding substrate,

as well as unique diluent/blocking and wash solutions.

See more with KPL ELISA Products

KPL ELISA products helpyou to optimize your assayand SEE MORE!

1.800.638.3167 www.kpl.com

Praying Mantis

See more with KPL ELISA products —Get higher signal and lower background.

Substrates are the cornerstones for

developing ELISAs with high signal

and low background. KPL, one of the

first companies to produce stable, liquid

substrates, offers a variety of standard

and proprietary HRP and AP substrates.

See Table 1 for a listing of chromogenic

and chemiluminescent substrates.

ChromogenicPeroxidase Substrates

Choose from among three TMB (3,3’,5,5’-

tetramethylbenzidine) products and two

ABTS (2,2”-azino-di-(3-ethylbenzthiazoline

-6-sulfonate) formulations. All provide

guaranteed performance and reliability.

Excellent Signal-to-Noise with SureBlueTM

• High sensitivity

• Consistent lot-to-lot performance

minimizes assay re-optimization

• 2-year stability

KPL’s SureBlue TMB Microwell Substrate is

a 1-component formulation. It develops a

deep blue soluble product when reacted

with HRP-labeled conjugates. SureBlue

TMB provides excellent signal-to-noise,

stability, and consistency, making it the

ideal substrate for use in most ELISA

applications.

Superior Sensitivity with

SureBlue Reserve

• Highest sensitivity: 50% more sensitive

than SureBlue TMB (Figure 1)

• Consistent lot-to-lot performance

minimizes assay re-optimization

• 2-year stability

• Ready-to-use 1-component

formulation

SureBlue Reserve TMB takes ELISA

to a new performance level. It offers

maximum sensitivity with exceptionally

low background enabling detection of

low levels of target protein. SureBlue

Reserve TMB provides the same great

benefits as KPL’s original SureBlue TMB:

low lot-to-lot variation, 2-year stability,

ready-to-use 1-component solution, and

excellent linearity.

TMB Microwell Substrate in a Highly

Stable 2-Component Formulation

• Longest shelf life due to the

2-component system

• Higher sensitivity than ABTS

TMB Microwell 2-Component Substrate

offers excellent stability and sensitivity.

It is the most economical choice when

ready-to-use convenience is not required.

Like other TMB substrates, it rapidly

develops a deep blue color that is

measurable at 650 nm.

Broad Dynamic Range with

ABTS Microwell Substrates

• Excellent linearity; provides the

widest working range of any

chromogenic HRP substrate

• Extremely stable

• Ideal for kinetic assays

KPL’s ABTS Microwell Substrates offer

performance, low background, and a

wider linear range than other chro-

mogenic substrates. Supplied either

as a ready-to-use formulation or as an

extremely stable 2-component product,

ABTS substrates develop an intense

blue-green color in the presence of

peroxidase-labeled conjugates. ABTS

is recommended when low background

is required and moderate sensitivity

is acceptable.

ELISA Substrates

Table 1. KPL Protein Detector ELISA Substrates

Sensitivity Stability Convenience Enzyme Detection Determination

SureBlue Reserve ••• ••• ••••• HRP Chromo Kinetic or Endpoint

SureBlue •• ••• ••••• HRP Chromo Kinetic or Endpoint

TMB 2-C •• •••• ••• HRP Chromo Kinetic or Endpoint

ABTS 1-C • •••• ••••• HRP Chromo Kinetic or Endpoint

ABTS 2-C • ••••• ••• HRP Chromo Kinetic or Endpoint

BluePhos •• •• ••• AP Chromo Kinetic or Endpoint

FirePhos •• •• ••• AP Chromo Kinetic or Endpoint

pNPP • ••••• • AP Chromo Kinetic or Endpoint

LumiGLO Reserve ••••• •• ••• HRP Chemi Endpoint

LumiGLO •••• •• ••• HRP Chemi Endpoint

PhosphaGLO •••• ••• ••••• AP Chemi Kinetic or Endpoint

PhosphaGLO Reserve ••••• ••• ••••• AP Chemi Kinetic or Endpoint

ELISA

C = Component • = Lowest ••••• = Highest

Chromogenic PhosphataseSubstrates

For economical chromogenic

phosphatase detection that provides

sensitivity and convenience, KPL offers

three substrates that produce a variety of

colors, new FirePhos, BluePhos® and

pNPP Substrates.

Excellent Overall Performance

with FirePhosTM and BluePhos®

• Ideal for applications where sensitivity

greater than pNPP is required

• Stable for 24 months

• Convenient and quick: mix and use

• Wider dynamic range than pNPP

• Lower background than pNPP

FirePhos and BluePhos Microwell

Substrates are based on a soluble, pro-

prietary form of 5-bromo-4-chloro-3-

indolyl phosphate (BCIP) and produce

intense colors (Figure 3). Both substrates

offer greater sensitivity and linearity of

signal than pNPP (Figure 2) and can be

used for both kinetic and endpoint

ELISAs.

Economy and Performance with pNPP

• Wide linear range

• Excellent stability

pNPP Phosphatase Substrate contains

para-nitrophenylphosphate. KPL’s formu-

lation provides one of the most sensitive

pNPP substrates available. Both kinetic and

endpoint determinations can be per-

formed. It is available in tablet form

with a separate buffer solution for shelf

stability.

ChemiluminescentSubstrates for Peroxidaseand Phosphatase

LumiGLO® and LumiGLO Reserve

Chemiluminescent Peroxidase

Substrates

• Unsurpassed sensitivity with

LumiGLO Reserve

• Excellent stability

• Fast ramp-up of signal

• Stable for 24 hours after mixing

• Light emission sustained for 1-2 hours

LumiGLO and LumiGLO Reserve sub-

strates are luminol-based. In the presence

of hydrogen peroxide, they are converted

to an excited intermediate dianion which

emits light on return to its ground state.

Both LumiGLO Reserve and LumiGLO

substrates offer rapid and sensitive detec-

tion. The working range of primary anti-

body that yields an acceptable signal-to-

noise ratio is much wider with these sub-

strates than with chromogenic substrates.

Both substrates are supplied as 2-component

liquids.

PhosphaGLOTM and PhosphaGLO

ReserveTM Chemiluminescent

Phosphatase Substrates

• Femtogram sensitivity with

PhosphaGLO Reserve

• One-component, ready-to-use

• Stable at 4o C for two years.

PhosphaGLO Reserve and PhosphaGLO

substrates provide great sensitivity and

unmatched convenience. No special

blockers are required to achieve low back-

ground.

Comparison of SureBlue and SureBlue Reserve

O.D.at

450nm

HRP Concentration (ng/mL)

SureBlue ReserveSureBlue

2.0

1.5

1.0

0.5

0.00 0.5 1 1.5 2

Comparison of AP Colorimetric Substrates for ELISA

Figure 1. Dilutions of HRP conjugate were added tomicroplate wells and the conjugate in each well reactedwith either SureBlue or SureBlue Reserve. The reactionswere stopped with TMB Stop Solution, and optical densityreadings were taken at 450 nm. Data points represent theaverage of triplicate samples at each HRP concentration.The data demonstrate greater sensitivity of SureBlue Reserveat low concentrations of HRP.

Figure 2. Human IgG was serially diluted in an ELISA plate anddetected with goat ani-human IgG labeled with alkaline phos-phatase. KPL’s substrates, FirePhos, BluePhos and pNPP wereadded to the plate. The reactions were stopped with APstopSolution and read at the recommended wavelength for eachsubstrate. The results indicate that FirePhos and BluePhosproduce a significantly higher signal-to-noise relative to pNPP.

1.800.638.3167 www.kpl.com

RedPhos BluePhos pNPP

Figure 3.A Choice of Colors for Phosphatase Detection

Signal:Background

Antigen Concentration (ng/mL)

BluePhosFirePhospNPP

20

15

10

5

00 200 400 600 800 1000

Choose from a wide selection of secondary

antibodies to immunoglobulins from over

20 animal species labeled with horseradish

peroxidase or alkaline phosphatase, the

most widely used enzymes for ELISA

signal generation. HRP has been shown

to be more sensitive than AP primarily

due to its faster catalytic rate. However,

HRP reactions may be self-limiting due

to substrate inhibition of enzyme. In

contrast, AP exhibits a slower catalytic

rate, but is not self-limiting. Reaction rates

remain linear over long periods of time;

therefore, sensitivity can be improved

by allowing the reaction to proceed for

a longer time.

Whatever enzyme conjugate you choose,

all antibody conjugates are of the same

exceptionally high quality you have come

to expect from KPL. As always, you get

results with high signal-to-noise.

Universal ELISA Kits

KPL’s Protein Detector ELISA Kits are

designed to be an excellent and convenient

starting point for development of ELISA

protocols. Each kit contains all the

reagents necessary to establish an ELISA

procedure except for primary antibodies.

The kits also include a comprehensive

manual to facilitate assay development.

HRP ELISA Kits

KPL’s Protein Detector HRP ELISA

Kits contain goat anti-mouse IgG and

anti-rabbit IgG, or anti-human IgG HRP

conjugates along with ABTS substrate,

blocking, coating, substrate, stop and

wash solutions.

AP ELISA Kits

Included in the Protein Detector

AP ELISA kits are goat anti-mouse

IgG, anti-rabbit IgG, or anti-human

IgG AP conjugates as well as BluePhos

substrate, blocking, coating, stop and

wash solutions.

ELISA Support Reagents

KPL’s support reagents are optimized

for use in microwell ELISA and tested to

verify that they meet strict specifications.

They include conjugate stabilizers,

diluents, blocking and wash solutions,

and stop reagents. Blocking and wash

solutions have been designed to reduce

background and to ensure a clear signal.

KPL stabilizers provide convenient storage

solutions for your antibody-enzyme

conjugates and ensure their performance.

Spend your time developing your assay

instead of worrying about making

reagents. Choose time-tested, stable

support reagents from KPL.

ELISA Antibody Conjugates See More...

Try KPL’s ELISA Kitsand Reagents

KPL has extensive experience

in antibody purification and the

development of stable liquid

substrates. This long-standing

expertise is the foundation of our

line of Protein Detector ELISA

systems and ensures reliable product

performance from lot-to-lot.

Try our kits and reagents and get the

results you’re looking for: a strong,

clean signal without background,

every time.

Your eyes aren’t playing tricks on

you. You’ve found the finest line of

ELISA systems and reagents at KPL.

LumiGLO and BluePhos are registered trade-

marks and LumiGLO Reserve, PhosphaGLO,

PhosphaGLO Reserve, SureBlue Reserve,

SureBlue, FirePhos, ReserveAP, BlueSTOP and

Protein Detector are trademarks of KPL, Inc.

ELISA

1.800.638.3167 www.kpl.com

• Antibody Sandwich (Capture) ELISA• Indirect Antibody ELISA• Direct ELISA• Hybridoma Screening

Protein DetectorTM ELISA Kit HRP System

offers a convenient starting point for the

development of microwell procedures for

antigen or antibody detection. The kit

provides pretested components including

HRP-labeled anti-mouse and anti-rabbit or

anti-human secondary antibodies along with

blocking, coating and wash solutions which

substantially reduce the time and effort need-

ed to develop a reproducible assay.

This kit also contains ABTS®, a peroxidase

substrate that provides reliability, conven-

ience and excellent overall performance. A

comprehensive manual for developing

enzyme immunoassay procedures is also pro-

vided. Users only need to provide primary

antibody and plates to run an ELISA.

Protein Detector ELISA kits offer the follow-

ing benefits:

Excellent Dynamic Range

Excellent linearity: ABTS provides the widest

working range of any chromogenic HRP sub-

strate.

Extremely Stable

All kit components are stabilized for ease-of-

use and reliability. Very low background,

long shelf life.

Convenient

Non-hazardous, no special handling or dis-

posal required. Optimized liquid stystem

makes your ELISA experiments faster and

easier. Ideal for use in a variety of ELISA

techniques.

Protein DetectorTM Horseradish Peroxidase (HRP) ELISA Kit

HRP ELISA Kits

SEE MORE with KPL!

0.000

0.100

0.200

0.300

0.400

0.500

500

250

125

62.5

31.3

15.6

7.8

ABTS unstopped

ABTS stopped

Performance of ABTS Substrate

Ab

sorb

ance

Conjugate Concentration (ng/mL)

Ordering Information

HRP ELISA Kits

Gaithersburg, MD 20878

Phone: 800.638.3167

Fax: 301.948.0169

www.kpl.com

Catalog No. Description Size

54-62-10 Protein Detector ELISA Kit, HRP System 2000 tests

Anti-Human Antibody


Anti-Mouse Antibody


Anti-Rabbit Antibody

Related Products

55-81-50 Protein Detector ELISA Kit, AP System 2000 tests

Anti-Mouse and Anti-Rabbit Antibodies


Anti-Human Antibody

To order or for more information on KPL’s full line of protein and nucleic acid detection

products, contact us at 800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at

www.kpl.com.

ML285-04

© 2003 KPL, Inc. All rights reserved.

Each kit contains:1. Coating Solution Concentrate

2. BSA Diluent/Blocking Solution Concentrate

3. Wash Solution Concentrate

4. 50% glyceral

5. HRP-labeled antibody, .1 mg

6. 2-component ABTS Substrate

7. Stop Solution Concentrate

8. Manual

Storage: Store kit reagents at 2 - 8° C.

Stability: Kit is stable for a minimum of 1 year

from date of receipt.

ABTS is a registered trademark of the Roche Group.

ABTS offers a broad working range prior to stoppingand after stopping.

Features of ABTS Substrate

Substrate

Type

Kit Components

Detection Limit

Kinetics

Stability of Working Solution

Hazards

Color Before Stopping

Absorbance Before Stopping

Stop Reagent

Color After Stopping

Absorbance After Stopping

Amplification After Stopping

ABTS

Chromogenic

2

10-13 moles of HRP

Slow

24 hours

None

Blue-Green

405-410 nm

ABTS Stop Solution

Blue-Green

405-410 nm

None

SEE MORE with KPL!

ISO 9001:2000 Registered

• Antibody Sandwich (Capture) ELISA• Indirect Antibody ELISA• Direct ELISA• Hybridoma Screening

Protein Detector AP ELISA Kits offer an

excellent and convenient starting point for

the development of ELISA protocols. These

kits provide pretested goat anti-mouse, anti-

rabbit or anti-human AP conjugates as well

as blocking, coating and wash solutions. KPL

kits substantially reduce the effort needed to

develop a sensitive and reproducible assay.

All components needed to establish an

ELISA except for primary antibodies are

provided.

AP ELISA kits utilize BluePhos®, a phos-

phatase substrate that provides superior per-

formance over pNPP. A comprehensive man-

ual for developing enzyme immunoassay

procedures is also provided.

Protein Detector AP ELISA kits offer the

following benefits:

Highly Sensitive

Detect at least 2 ng of protein and less than

0.5 pg of phosphatase in free solution.

Extremely Stable

Very low background, long shelf life.

Excellent Dynamic Range

BluePhos offers improved signal-to-noise

ratio and linearity over pNPP.

Convenient

Optimized liquid system makes your ELISA

experiments faster and easier. Non-

hazardous, no special handling or disposal

required. Ideal for use in a variety of ELISA

techniques.

Protein DetectorTM Alkaline Phosphatase (AP) ELISA Kits

AP ELISA Kits

SEE MORE with KPL!


Methodology

Mouse IgG was coated ontotwo different ELISA plates.The starting dilution for theBluePhos plate was 1 mg/mL,while the pNPP plate started at10 μg/mL. The antigen wasdiluted 2-fold eight times.Phosphatase-labeled goat anti-mouse IgG was added to eachwell at a concentration of 500ng/mL. The plate was washed,and substrate applied. Basedon the background readings attime 0, and the final readingsat time 15 minutes, theamount of protein was deter-mined by dividing the dilutionconcentration by 100 μL (thevolume used when coating thewell). Therefore, the amountof protein detected byBluePhos (point 7) is 1.56 ng,while the amount detected bypNPP (point 5) is 62.4 ng.

Conclusion

The graph shows thesuperiority of BluePhos inboth increased sensitivity andin producing a wider dynamicrange than pNPP.

AP ELISA Kits


Phone: 800.638.3167

Fax: 301.948.0169

www.kpl.com

Catalog No. Description Size


Anti-Mouse and Anti-Rabbit Antibodies


Anti-Human Antibody

Related Products


Anti-Human Antibody


Anti-Mouse Antibody


Anti-Rabbit Antibody

Each kit contains: Coating Buffer Concentrate, BSA Diluent/Blocking Solution Concentrate, Wash SolutionConcentrate, 50% Glycerol Solution, Enzyme-labeled Secondary Antibody, 2-Component Chromogenic SubstrateSystem (Substrate components may vary depending upon kit.), and Stop Solution.

To order or for more information on KPL’s full line of protein and nucleic acid detection

products, contact us at 800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at

www.kpl.com.

0.5

1.0

1.5

2.0

2.5

3.0

Performance Comparison of BluePhos and pNPP

O.D

.

Natural Log (Dilution)

01 2 876543

BluePhos

pNPP

ML271-06

BluePhos is a registered trademark and Protein Detector is a trademarks of KPL, Inc.© 2003 KPL, Inc. All rights reserved.

SEE MORE with KPL!


Lighten your work load with KPL’s new line of quality

buffers. Offered as convenient liquid concentrates, they

eliminate the need for time-consuming buffer preparation.

KPL buffers are manufactured and carefully controlled for

quality and consistent performance with our ISO 9001:2008-

registered quality management system. All buffers are con-

ductivity controlled. KPL offers a variety of buffers designed

for use in general immunoassay applications such as Western

blotting, gel electrophoresis, ELISA and sample preparation.

Save preparation time with KPL’s new line of quality buffers!

Immunoassay Buffers

SEE MORE with KPL!

Description 1X Composition Applications

10X Tris-Glycine 25 mM Tris, 192 mM glycine. For preparing a standard Western blot transfer buffer Transfer Buffer pH 8.3 (Towbin) and as a gel electrophoresis buffer for native

Tris-glycine gels without SDS. .

10X Tris-Glycine-SDS 25 mM Tris, 192 mM glycine, Running buffer for sodium dodecyl sulfate – polyacrylamide0.1% SDS. pH 8.3 gel electrophoresis (SDS-PAGE) of proteins.

10X Phosphate-Buffered 10 mM Phosphate,150 mM NaCl. Wash buffer for general immunoassay applicationsSaline (PBS) pH 7.4 and sample preparation. Where applicable, Tween 20

is a nonionic detergent that reduces nonspecificPhosphate-Buffered 10 mM Phosphate, 150 mM NaCl, binding and protein-protein interaction during wash steps.Tween 20, (PBST) 0.05% Tween 20. pH 7.4

10X Tris-Buffered Saline 50 mM Tris, 150 mM NaCl. (TBS) pH 7.6

10% Tween 20 10% Tween 20 Nonionic detergent additive for PBS and Western blotting wash solutions. Reduces nonspecific binding and protein- protein interactions.

See reverse side.

Catalog# Description Size

51-10-01 10X Tris-Glycine Transfer Buffer 1 L

51-10-02 5 L

51-11-01 10X Tris-Glycine SDS Buffer 1 L

51-11-02 5 L

51-13-01 10X Phosphate-Buffered Saline (PBS) 1 L

51-13-02 5 L

51-14-01 10X Phosphate-Buffered Saline with 200 mL

51-14-02 Tween 20 (PBST) 1 L

51-17-01 10X Tris-Buffered Saline (TBS) 1 L

51-17-02 5 L

51-12-01 10% Tween 20 200 mL

51-12-02 1 L

51-20-01 5% SDS 200 mL

50-86-05 20X SSC 1 L

51-15-01 10X Dulbecco's PBS (D-PBS) 1 L

51-15-02 5 L

51-16-01 10X Dulbecco's PBS with Tween 20 (D-PBST) 200 mL

51-16-02 1 L

51-18-01 10X Tris Buffered Saline with 0.5% Tween 20 200 mL

51-18-02 (TBST) 1 L

51-19-01 10X Tris Buffered Saline with 10% Tween 20 200 mL

51-19-02 (TBST) 1 L


To order or for more information on KPL’s full line of protein detection products, contact us at800.638.3167 / 301.948.7755, fax 301.948.0169 or visit us at www.kpl.com.

Other Immunoassay Support

Reagents Available from KPL

ELISA and Western Blotting

Applications:

• AP and HRP Conjugate Stabilizers

• 10% BSA Diluent/Blocking Solution

Concentrate

• Milk/Diluent Blocking Solution

Concentrate

• 20X Wash Solution Concentrate

Western Blotting Applications:

• Detector Block

• Biotin Wash Kit

ELISA Applications:

• Coating Solution Concentrate

• Stop Solutions

Immunoassay Buffers


Phone: 800.638.3167

Fax: 301.948.0169

www.kpl.com

ML363-03

©2009 KPL, Inc. All rights reserved.SEE MORE with KPL!


KPL Quality Buffers (cont’d)

Take the time and effort out of your buffer preparation. Choose from our line of popular buffers andenjoy the benefits - convenience, reliability and confidence - that KPL’s pretested, quality buffersbring to your research.

Description 1X Composition Applications

5% SDS 5% Sodium Detergent surfactant used in preparing proteins for Dodecyl Sulfate sodium dodecyl sulfate – polyacrylamide gel electro-

phoresis (SDS-PAGE) and as an additive to transfer buffers in Western blotting. SDS increases the elution rate of proteins from the gel.

20X SSC 15 mM sodium citrate, Used in a variety of molecular biology applications. 150 mM NaCl Facilitates transfer of nucleic acids to membranes. in DEPC water. pH 7.0

10X Dulbecco's 8.5 mM sodium phosphate, For sample preparation and as a wash buffer in general PBS (D-PBS) 1.5 mM potassium phosphate, immunoassay, tissue, and cell culture applications.

137 mM NaCl. pH 7.4 Not formulated with magnesium or calcium salts. Where applicable, Tween 20 is a non-ionic detergent that reducesnonspecific binding.

10X Dulbecco's 8.5 mM sodium phosphate,with Tween 20. 1.5 mM potassium phosphate,(D-PBST) 137 mM NaCl, 2.7 mM KCl.

0.05% Tween 20. pH 7.4

10X Tris-Buffered 50 mM Tris, 150 mM NaCl, As a general wash buffer in immunoassay applications. Saline with 0.5% 0.05% Tween 20. pH 7.6 Tween 20 is a non-ionic detergent that reduces nonspecific Tween 20 (TBST) binding. The 10% Tween 20 formulation provides a more

stringent wash than standard TBST formulations. 10X Tris-Buffered 50 mM Tris, 150 mM NaCl,Saline with 10% 1.0% Tween 20. pH 7.6 Tween 20 (TBST)

The success of an ELISA (enzyme-linked immunosor-

bent assay) depends on a number of factors, includ-

ing the performance characteristics of the substrate.

KPL’s line of peroxidase (HRP) color substrates pro-

vides a range of options to match individual perform-

ance requirements, including high signal and sensitiv-

ity, low background, stability and convenience.

As the most preferred peroxidase substrate, TMB

(3,3’,5,5’ tetramethylbenzidine) provides high sensi-

tivity and low background in microwell immunoas-

says. KPL offers a choice of three TMB substrates,

starting with SureBlueTM (1-Component), ideal for

most ELISA applications. Our TMB Peroxidase

Substrate (2-Component) provides equivalent sensi-

tivity at an economical price. At the high end of sen-

sitivity, SureBlue ReserveTM (1-Component ) is the

substrate of choice when ultimate performance is

required. All three substrates are recommended for

both kinetic and endpoint ELISA. Upon reaction

with peroxidase, they turn blue and are read at 650

nm.

KPL’s ABTS (2,2’-azino-di-(3-ethylbenzthiazoline-6-

sulfonate) peroxidase substrates offer reliable per-

formance and low background in ELISA. They turn

an intense blue-green in the presence of peroxidase

that is measureable between 405 and 415 nm.

Choose the substrate that suits your needs:

• SureBlue ReserveTM TMB SubstrateSureBlue Reserve provides maximum sensitivity while

maintaining low background, high consistency and

24-month stability in a convenient 1-component for-

mulation.

• SureBlueTM TMB Microwell SubstrateSureBlue TMB offers high sensitivity, consistency and

stability in a convenient 1-component solution.

• TMB Peroxidase SubstrateTMB Peroxidase Substrate offers equivalent perform-

ance to SureBlue in an easy mix-and-use, 2-component

formulation.

• ABTS Peroxidase SubstrateABTS substrates are supplied as stable 1- and 2-com-

ponent formulations. They are recommended when

low background is required and moderate sensitivity

is acceptable.

Reliable performance and a range of options

HRP ELISA Color Substrates

SEE MORE with KPL!

As the graph above demonstrates, our TMB substrates (SureBlue Reserve, SureBlue and TMB)produce high O.D. values, with SureBlue Reserve exhibiting the highest sensitivity. SureBlue andTMB demonstrate lower O.D. values and are comparable in performance. ABTS 1- and 2-compo-nent yield lower O.D. values than TMB while exhibiting similar performance. In summary, KPL’scolorimetric substrates provide a range of sensitivity options to meet a variety of needs.

Comparison of KPL’s HRP Microwell Substrates


To order or for more information on KPL’s line of protein detection products, contact

us at 800.638.3167 / 301.948.7755, fax 301.948.0169 or visit us at www.kpl.com.

Catalog# Description Size52-00-01 SureBlue TMB Microwell Substrate (1-Component) 100 mL

52-00-02 SureBlue TMB Microwell Substrate (1-Comp) 400 mL

52-00-03 SureBlue TMB Microwell Substrate (1-Comp) 1 L

53-00-01 SureBlue Reserve TMB Microwell Substrate (1-Comp) 100 mL

53-00-02 SureBlue Reserve TMB Microwell Substrate (1-Comp) 400 mL

53-00-03 SureBlue Reserve TMB Microwell Substrate (1-Comp) 1 L

50-76-00 TMB Microwell Substrate (2-Comp) 600 mL

50-76-03 TMB Microwell Substrate (2-Comp) 2700 mL

50-66-18 ABTS Microwell Substrate (1-Comp) 100 mL

50-66-00 ABTS Microwell Substrate (1-Comp) 600 mL

50-66-06 ABTS Microwell Substrate (1-Comp) 1 L

50-62-00 ABTS Substrate System (2-Comp) 600 mL

50-62-01 ABTS Substrate System (2-Comp) 2700 mL

Stop Solutions50-85-30 TMB BlueSTOP Solution 400 mL

50-85-41 TMB BlueSTOP Solution 1 L

50-85-05 TMB Stop Solution 400 mL

50-85-06 TMB Stop Solution 1 L

50-85-01 ABTS Stop Solution 200 mL

• TMB BlueSTOPTM

- formulated to stop color develop-

ment in microwell assays and to

maintain the blue color of TMB.

Unlike traditional acid-based TMB

stopping solutions, the O.D. value

of TMB does not change upon

addition of TMB BlueSTOP. The

stopped substrate is stable for an

extended period for reading at 650

nm. A single 650 nm filter can be

used pre- and post-stopping.

• TMB Stop Solution

- changes the blue color of TMB

substrate to yellow and increases

absorbance values 2 - 3 times.

Stopped TMB is read at a wave-

length of 450 nm.

• ABTS Stop Solution

- specifically designed for use with

KPL’s ABTS Peroxidase Substrates

to stop color development in

micro-well assays. ABTS retains the

green color after stopping.

TMB Stop Solutions


Phone: 800.638.3167

Fax: 301.948.0169

www.kpl.com

SureBlue, SureBlue Reserve and TMB BlueSTOP are trademarks of KPL. ABTS is a registered trademark of Roche Biochemicals.©2008 KPL, Inc. All rights reserved.ML352-02

SEE MORE with KPL!


Prefer vibrant red or brilliant blue? Choose

between new FirePhosTM, BluePhos® or pNPP

AP Microwell Substrates for sensitive ELISA

detection! KPL offers a choice of three sensitive

substrates for the detection and quantification

of phosphatase (AP) activity in microwell assays.

New FirePhosTM AP Microwell Substrate

BluePhos® AP Microwell Substrate

pNPP Phosphatase Substrate

Whichever substrate you choose, enjoy the

benefits of excellent signal-to-noise with higher

sensitivity and lower background than that of

other AP microwell substrates. They produce

strong colors that can be read visually. When

combined with KPL’s APstopTM Solution, signal

development is completely stopped for end-

point determinations. The fast rate of color gen-

eration also permits accurate, quantitative meas-

urement in kinetic ELISA.

Extended stabilityAll three AP substrates are stable for one year.

ConvenienceFirePhos and BluePhos are offered as liquid

two-component solutions. To prepare mix

equal parts and use.

SensitivityFirePhos and BluePhos provide greater sensi-

tivity than pNPP and can detect less than 0.5

pg of phosphatase. (See data on reverse side.)

KPL’s pNPP substrate is one of the most sensi-

tive pNPP formulations available, showing

enhanced performance over competitive prod-

ucts. The color produced is stable and linear

over a wide dynamic range.

ReproducibilityAll KPL AP colorimetric substrates are opti-

mized for consistent results from lot-to-lot,

assay-to-assay.

See your ELISA results in vivid colors!

SEE MORE with KPL!

AP ELISA Color Substrates

Comparison of AP colorimetric substrates for ELISAHuman IgG was serially diluted in an ELISA plate and detected with goat anti-human IgG labeledwith alkaline phosphatase. KPL’s substrates, FirePhos, BluePhos, pNPP and a competitor pNPPwere added to the plate. The reactions were stopped with APstop Solution and read at the recom-mended wavelength for each substrate. The results indicate that FirePhos and BluePhos produce asignificantly higher signal-to-noise relative to pNPP.

Phosphatase Substrates for ELISA


To order or for more information, contact us at 800.638.3167 / 301.948.7755,

fax 301.948.0169 or visit us at www.kpl.com.


FirePhosTM AP Microwell Substrate

50-87-10 FirePhos Microwell Phosphatase Substrate System 50 mL



50-87-40 FirePhos Microwell Phosphatase Substrate System 2.7 L

BluePhos® AP Microwell Substrate

50-88-02 BluePhos Phosphatase Substrate for ELISA 50 mL



pNPP Phosphatase Substrates

50-80-00 pNPP Phosphatase Substrate Kit, 100 tabs & DEA Buffer 500 mL

50-80-01 pNPP Substrate, 5 mg tablets 100 tabs

Stop Solutions

50-89-00 APstop Solution (10X) (formerly BluePhos Stop Solution) 200 mL

ReserveAPTM

Conjugates

ML347-02

For research use only.BluePhos is a trademark of KPL and is covered by U.S. Patent No. 5,916,746.© 2007 KPL, Inc. All rights reserved.

Improve your assay performance even furtherwith high potencyReserveAPTM Antibodies

KPL’s new ReserveAPTM Alkaline

Phosphatase-labeled Conjugates

exhibit high potency and consis-

tent performance in immunoas-

says. These conjugates are the

result of advances in our conjuga-

tion technology and offer higher

signal and lower background than

our current line of alkaline phos-

phatase conjugates while meeting

the same standards for stability

and reproducibility. They are ideal

for immunoassays that require

high detection sensitivity in an AP

system, including ELISA, Western

blotting and immunohistology.


Phone: 800.638.3167

Fax: 301.948.0169

www.kpl.com


SEE MORE with KPL!

KPL’s new ReserveAPTM Alkaline Phosphatase

(AP)-labeled antibody conjugates exhibit high

potency and consistent performance in

immunoassays. These conjugates are the result

of advances in our conjugation technology and

offer higher signal than our current line of AP

conjugates while meeting the same standards

for low background, stability and reproducibili-

ty. They are intended for demanding

immunoassays that require high detection sen-

sitivity, including ELISA, Western blotting and

immunohistology.

Higher Potency

ReserveAP Conjugates are affinity purified and

conjugated to the highest grade of alkaline

phosphatase. In our studies, they generate two-

to-three fold higher values than our current

line of AP conjugates in ELISA and outperform

AP conjugates offered by other manufacturers.

Higher conjugate dilutability is also observed

without loss of linearity, enabling precious

antigen or primary antibody conservation.

Consistent Performance

Reproducible antibody conjugation and consis-

tent performance are verified according to our

ISO 9001:2000-registered quality assurance

system. Lot consistency studies in which three

lots were studied by ELISA indicated minimal

variability.

Excellent Value

ReserveAP Conjugates provide high perform-

ance at an economical price.

Spice up your assay with our red hot high potency ReserveAPTM Conjugates!

ReserveAPTM Conjugates

SEE MORE with KPL!

ReserveAPTM Conjugates Ordering Information


0751-1001 Goat Anti-Human IgA (α) 1.0 mg

0751-1002 Goat Anti-Human IgG (γ) 1.0 mg

0751-1003 Goat Anti-Human IgM (µ) 1.0 mg

0751-1004 Goat Anti-Human IgE (ε) 1.0 mg

0751-1006 Goat Anti-Human IgG (H+L) 1.0 mg

0751-1007 Goat Anti-Human IgA+IgG+IgM (H+L) 1.0 mg

2151-1002 F(ab’)2 Anti-Human IgG (γ) 0.5 mg

4751-1002 Goat Anti-Human IgG (γ) liquid 1.0 mg

4751-1003 Goat Anti-Human IgM (µ) liquid 1.0 mg

4751-1006 Goat Anti-Human IgG (H+L) liquid 1.0 mg

0751-1802 Goat Anti-Mouse IgG (γ) HSA 1.0 mg

0751-1803 Goat Anti-Mouse IgM (µ) HSA 1.0 mg

0751-1806 Goat Anti-Mouse IgG (H+L) HSA 1.0 mg

0751-1809 Goat Anti-Mouse IgG +IgM (H+L) HSA 1.0 mg

4751-1802 Goat Anti-Mouse IgG (γ) HSA, liquid 1.0 mg

4751-1806 Goat Anti-Mouse IgG (H+L) HSA, liquid 1.0 mg

151-18-01 Goat Anti-Mouse IgA (α) HSA 0.5 mg

0751-1807 Goat Anti-Mouse IgA+IgG+IgM (H+L) HSA 1.0 mg

0751-1506 Goat Anti-Rabbit IgG (H+L) 1.0 mg

0751-1516 Goat Anti-Rabbit IgG (H+L) HSA 1.0 mg

4751-1506 Goat Anti-Rabbit IgG (H+L), liquid 1.0 mg

4751-1516 Goat Anti-Rabbit IgG (H+L) HSA, liquid 1.0 mg

HSA=Human Serum Adsorbed

AP Substrates


Phone: 800.638.3167

Fax: 301.948.0169

www.kpl.com


ML349-04

For research use only.©2007 KPL, Inc. All rights reserved.

SEE MORE with KPL!

Visit our website at www.kpl.com for a complete listing of ReserveAP conjugates. To

order or for more information on KPL’s protein research products, contact us at

800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at www.kpl.com.

KPL offers a range of sensitive sub-

strates for the detection and quantifi-

cation of phosphatase (AP) activity.

They provide a choice of intense col-

ors for ELISA and blotting applica-

tions.

ELISA

• FirePhosTM AP Microwell

Substrate

• BluePhos® AP Microwell

Substrate

• pNPP Phosphatase Substrate

Blotting

• FirePhos AP Membrane

Substrate

• BCIP/NBT Phosphatase Substrate

• PhosphaGLO AP Substrate

• PhosphaGLOTM Reserve AP

Substrate

Whichever substrate you choose,

enjoy the benefits of excellent signal-

to-noise with higher sensitivity and

lower background than that of other

AP substrates. Visit www.kpl.com

for more information.

Chemiluminescent ELISA: A con-stant amount of antigen (mouse IgG)was coated onto a microtiter plate.The plate was probed with varyingconcentrations of goat anti-mouseIgG (H+L) AP conjugate, includingKPL’s standard AP conjugate andReserveAP™ conjugate, followed byKPL’s PhosphaGLO™ Chemilum-inescent AP Substrate.

Conclusion: The results indicate that ReserveAP™ conjugate offers superior performance to our stan-dard AP conjugate with a ~3-fold lower amount of conjugate required for detection.

KPL produces a unique line of BacTrace® anti-

bacteria antibodies for the detection of food

pathogens and other infectious agents affecting

humans and animals. They are purified to

offer excellent specificity and sensitivity in

high performance immunoassays such as

ELISA, Western blotting, lateral flow and

microscopic analysis. Many leading diagnostic

kit manufacturers use BacTrace antibodies in

their diagnostic kits. See our growing line of

BacTrace antibodies:

• Anti-Salmonella

• Anti-Salmonella, Molecular Grade

• Anti-E.coli O157:H7

• Anti-E.coli O157:H7, Molecular Grade

• Anti-Listeria, genus-specific

• Anti-Campylobacter, genus specific

• Anti-Helicobacter pylori

• Anti-Borrelia species

• Anti-Borrelia burgdorferi

• Anti-Renibacterium salmoninarum

BacTrace antibodies offer a range of benefits:

• Sensitivity: nanogram detection of bacterial

antigens within hours.

• Specificity: recognize both cell surface and

intracellular antigens of bacterial cells.

• Versatility: ideal for screening assays in the

research lab, environmental monitoring and

for food and beverage analysis in the indus-

trial setting.

• Positive controls available for each

BacTrace antibody.

New molecular grade antibodies to Salmonella

and E. coli O157:H7 are recent additions to our

BacTrace line. These antibodies are specially

processed and QC-tested to ensure that con-

taminating Salmonella or E. coli DNA is not

detectable by real-time PCR or other molecular

methods.

BacTrace Antibodies to Food Pathogens and Other Infectious Agents

BacTrace® Anti-Bacteria AntibodiesBacTrace® E.coli O157:H7 Antibody labeled with DyLight 488 (green) directly detects E.coli O157:H7. BacTrace Salmonella (CSA-1) Antibody labeled with DyLight549 (red) directly detects Salmonella. (DyLight labeling performed on a custom basis.)

SEE MORE with KPL!

Anti-Salmonella and E.coli O157:H7, Molecular Grade


To order or for more information on KPL’s line of protein detection products, contact usat 800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at www.kpl.com.


Anti-Salmonella (CSA-1) Common Structural Antigen01-91-99 Unlabeled 1.0 mg01-90-99-MG Unlabeled, molecular grade 1.0 mg05-91-99 Phosphatase-labeled 0.1 mg 04-91-99 Peroxidase-labeled 0.1 mg02-91-99 Fluorescein-labeled 0.5 mg50-74-01 Salmonella typhimurium Positive Control Antigen 1.0 mL

Anti-E. coli O157:H701-95-90 Unlabeled 1.0 mg01-95-90-MG Unlabeled, molecular grade 1.0 mg05-95-90 Phosphatase-labeled 0.1 mg 04-95-90 Peroxidase-labeled 0.1 mg02-95-90 Fluorescein-labeled 0.5 mg50-95-90 E. coli O157:H7 Positive Control Antigen 1.0 mL

Anti-Listeria, genus specific01-90-90 Unlabeled 1.0 mg05-90-90 Phosphatase-labeled 0.1 mg 04-90-90 Peroxidase-labeled 0.1 mg02-90-90 Fluorescein-labeled 0.5 mg50-90-90 Listeria, genus specific Positive Control Antigen 1.0 mL

Anti-Campylobacter, genus specific01-92-93 Unlabeled 1.0 mg05-92-93 Phosphatase-labeled 0.1 mg 04-92-93 Peroxidase-labeled 0.1 mg02-92-93 Fluorescein-labeled 0.5 mg50-92-93 Campylobacter jejuni Positive Control Antigen 1.0 mL

Anti-Borrelia burgdorferi01-97-91 Unlabeled 1.0 mg05-97-91 Phosphatase-labeled 0.1 mg 04-97-91 Peroxidase-labeled 0.1 mg02-97-91 Fluorescein-labeled 0.5 mg

Anti-Borrelia species01-97-92 Unlabeled 1.0 mg04-97-92 Peroxidase-labeled 0.1 mg02-97-92 Fluorescein-labeled 0.5 mg50-97-91 Borrelia burgdorferi Positive Control Antigen 1.0 mL

Anti-Helicobacter pylori01-93-94 Unlabeled 1.0 mg04-93-94 Peroxidase-labeled 0.1 mg02-93-94 Fluorescein-labeled 0.5 mg50-93-94 Helicobacter pylori Positive Control Antigen 1.0 mL

Anti-Renibacterium salmoninarum01-96-91 Unlabeled 1.0 mg05-96-91 Phosphatase-labeled 0.1 mg 04-96-91 Peroxidase-labeled 0.1 mg02-96-91 Fluorescein-labeled 0.5 mg50-96-91 R. salmoninarun Positive Control Antigen 1.0 mL

BacTrace®


Phone: 800.638.3167

Fax: 301.948.0169

www.kpl.com

ML366-01

For research use only.BacTrace is a registered trademark of KPL © 2008 KPL, Inc. All rights reserved.

SEE MORE with KPL!

ISO 9001:2000 RegisteredAll BacTrace antibodies are made in goat.

KPL has a large production capacity and offers flexible services to meetyour specific needs.

• Multi-gram quantities from the same purification lot• Labeling options, including peroxidase, phosphatase, fluorescein• Customized antibody formulations to tailor specificity and sensitivity• Custom conjugation to other reporter molecules (fluorophores, enzymes, etc.)

Anti-Salmonella (CSA)

•Broadly reactive; detects 23 Salmonella species

•Optimized for greatest specificity to Salmonella groups B,C,D and E

•Molecular grade antibody avail-able for testing by PCR or other molecular methods.

•Salmonella typhimurium positive control available

Anti-E.coli O157:H7

•Highly specific; minimal cross-reactivity to non-O157 serotypes of E.coli

•Molecular grade antibody avail-able for testing by PCR or other molecular methods

•E.coli O157:H7 positive control available

Since 1979 KPL has provided quality affinity

purified antibodies to researchers worldwide.

Over the years we have refined our production

process to provide antibodies with high potency

and consistent performance in immunoassays.

From the start KPL gives careful consideration

to immunogen preparation, using a highly

purifed formulation to generate antiserum. KPL

pools antiserum from multiple animals to

reduce natural animal to animal serum variabili-

ty. During the purification process our ISO

9001:2008-certified quality procedures impose

rigorous standards for potency and cross-reac-

tivity. The result is standardized antibody lots

with excellent reproducibility.

Our extensive line of peroxidase (HRP) conju-

gates is available across a range of animal

species, including human, mouse, rabbit and rat

antibodies, as well as other animal species and

streptavidin. They are affinity purified and in

some cases further adsorbed to minimize cross-

reactivity between animal species or shared

reactivity with other immunoglobulin classes.

HRP-labeled F(ab’)2 fragment antibodies are

offered for assays requiring extremely low back-

ground and absence of F(c)-mediated binding.

KPL reacts HRP of the highest quality with

affinity purified antibodies and streptavidin

using the periodate method of Nakane and

Kawaoi. Special features of HRP include:

• faster catalytic rate than alkaline phosphatase

• generates more product in shorter incubation

times

• provides maximum sensitivity, low nonspe-

cific binding

• ideal for ELISA, Western blotting and

immunohistology applications.

KPL Peroxidase Conjugates: Time-tested, Sensitive and Reliable

Peroxidase Conjugates

SEE MORE with KPL!

Peroxidase Conjugates Ordering Information(Partial listing)

Peroxidase Conjugates


Phone: 800.638.3167

Fax: 301.948.0169

www.kpl.com


For research use only.©2009 KPL, Inc. All rights reserved.ABTS is a registered trademark of Roche Biochemicals.ML371-01

SEE MORE with KPL!

Visit our website at www.kpl.com for a complete listing of HRP-labeled antibodies.

To order or for more information on KPL’s protein research products, contact us at

800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at www.kpl.com.

KPL offers a range of sensitive

substrates for use with HRP conju-

gates. They provide a choice of

intense colors for ELISA, blotting

and cell staining applications.

ELISA

• ABTS® 1- and 2-Component

Microwell Peroxidase Substrates

• SureBlueTM TMB Peroxidase

Substrate

• SureBlue ReserveTM TMB

Peroxidase Substrate

• TMB Peroxidase Substrate

Blotting

• 4 CN Peroxidase Substrate

• TMB Membrane Peroxidase

Substrate

• LumiGLO®Chemiluminescent

Substrate

• LumiGLO ReserveTM

Chemiluminescent Substrate

Whichever substrate you choose,

enjoy the benefits of excellent signal-

to-noise and reproducibility.


04-10-06 HRP-labeled Goat Anti-Human IgG (H+L) 0.1 mg

04-10-17 HRP-labeled Goat Anti-Human IgA+IgG+IgM (H+L), MSA 0.1 mg

04-10-20 HRP-labeled Goat Anti-Human IgG (Fc) 0.1 mg

074-1002 HRP-labeled Goat Anti-Human IgG (γ) 1.0 mg

074-1003 HRP-labeled Goat Anti-Human IgM (μ) 1.0 mg

074-1004 HRP-labeled Goat Anti-Human IgE (ε) 1.0 mg

074-1006 HRP-labeled Goat Anti-Human IgG (H+L) 1.0 mg

074-1007 HRP-labeled Goat Anti-Human IgA+IgG+IgM (H+L) 1.0 mg

14-10-01 HRP-labeled Goat Anti-Human IgA (α) 0.5 mg

214-1002 HRP-labeled F(ab’)2 Goat Anti-Human IgG (γ) 0.5 mg

214-1003 HRP-labeled F(ab’)2 Goat Anti-Human IgM (μ) 0.5 mg

214-1006 HRP-labeled F(ab’)2 Goat Anti-Human IgG (H+L) 0.5 mg

474-1002 HRP-labeled Goat Anti-Human IgG (γ), Liquid 1.0 mL

474-1003 HRP-labeled Goat Anti-Human IgM (μ), Liquid 1.0 mL

474-1006 HRP-labeled Goat Anti-Human IgG (H+L), Liquid 1.0 mL

04-18-06 HRP-labeled Goat Anti-Mouse IgG (H+L), HSA 0.1 mg

04-18-15 HRP-labeled Goat Anti-Mouse IgG (H+L), RtSA, HSA 0.1 mg

04-18-18 HRP-labeled Goat Anti-Mouse IgG (H+L), RbSA, HSA 0.1 mg

074-1802 HRP-labeled Goat Anti-Mouse IgG (γ), HSA 1.0 mg

074-1803 HRP-labeled Goat Anti-Mouse IgM (μ), HSA 1.0 mg

074-1806 HRP-labeled Goat Anti-Mouse IgG (H+L), HSA 1.0 mg

074-18-061 HRP-labeled Goat Anti-Mouse IgG (H+L), XSA 1.0 mg

074-1807 HRP-labeled Goat Anti-Mouse IgA+IgG+IgM (H+L), HSA 1.0 mg

074-1809 HRP-labeled Goat Anti-Mouse IgG+IgM (H+L), HSA 1.0 mg

14-18-01 HRP-labeled Goat Anti-Mouse IgA (α), HSA 0.5 mg

214-1802 HRP-labeled F(ab’)2 Goat Anti-Mouse IgG (γ), HSA 0.5 mg

214-1806 HRP-labeled F(ab’)2 Goat Anti-Mouse IgG (H+L), HSA 0.5 mg

474-1802 HRP-labeled Goat Anti-Mouse IgG (γ), HSA, Liquid 1.0 mL

474-1806 HRP-labeled Goat Anti-Mouse IgG (H+L), HSA, Liquid 1.0 mL

074-1506 HRP-labeled Goat Anti-Rabbit IgG (H+L) 1.0 mg

074-15-061 HRP-labeled Goat Anti-Rabbit IgG (H+L), XSA 1.0 mg

074-1516 HRP-labeled Goat Anti-Rabbit IgG (H+L), HSA 1.0 mg

214-1516 HRP-labeled F(ab’)2 Goat Anti-Rabbit IgG (H+L), HSA 0.5 mg

474-1506 HRP-labeled Goat Anti-Rabbit IgG (H+L), Liquid 1.0 mL

474-1516 HRP-labeled Goat Anti-Rabbit IgG (H+L), HSA, Liquid 1.0 mL

14-30-00 HRP-labeled Streptavidin 0.5 mg

474-3000 HRP-labeled Streptavidin, Liquid, Molecular Grade 1.0 mL


Catalog # Description Size

Protein Detector ELISA Kits

Each kit includes an AP- or HRP-labeled Conjugate, CoatingSolution, Wash Solution Concentrate, 10% BSA Diluent/BlockingSolution Concentrate, Stop Solution and Substrate.

Phosphatase

55-81-10 AP ELISA Kit, Anti-Human 20 plates

55-81-50 AP ELISA Kit, Anti-Rabbit andAnti-Mouse 20 plates

Peroxidase

54-62-10 HRP ELISA Kit, Anti-Human 20 plates

54-62-18 HRP ELISA Kit, Anti-Mouse 20 plates

54-62-15 HRP ELISA Kit, Anti-Rabbit 20 plates

Substrates for ELISA

Phosphatase Chromogenic Substrates

50-88-02 BluePhos Microwell Substrate Kit 50 mL

50-88-00 Same as above 600 mL

50-88-01 Same as above 2.7 L

50-87-10 FirePhos Microwell Substrate Kit 50 mL




50-80-00 pNPP Phosphatase Substrate System 500 mL

50-80-01 pNPP Phosphatase Tablets 100 tablets

Peroxidase Chromogenic Substrates

52-00-01 SureBlue TMB1-Component Substrate 100 mL


52-00-03 Same as above 1 L

53-00-01 SureBlue Reserve TMB1-Component Substrate 100 mL



50-76-00 TMB 2-Component Substrate Kit 600 mL


50-66-18 ABTS Microwell Substrate1-Component 100 mL



50-62-00 ABTS Substrate System2-Component 600 mL


Peroxidase Chemiluminescent Substrates

54-61-00 LumiGLO ChemiluminescentSubstrate Kit 240 mL



54-71-00 LumiGLO ReserveChemiluminescent Substrate Kit 2400 cm2

54-71-01 Same as above 600 cm2

Catalog # Description Size

Phosphatase Chemiluminescent Substrates

55-60-03 PhosphaGLO AP Substrate 30 mL


55-60-01 PhosphaGLO Reserve AP Substrate 30 mL


Antibody Conjugates

All antibodies listed below are produced in goat. For a completeantibody listing, visit www.kpl.com or refer to KPL's ProductCatalog.

Phosphatase

075-1006 ReserveAP Anti-Human IgG (H+L) 1.0 mg

075-1806 ReserveAP Anti-Mouse IgG (H+L), HSA 1.0 mg

075-1506 ReserveAP Anti-Rabbit IgG (H+L) 1.0 mg

Peroxidase

074-1006 Anti-Human IgG (H+L) 1.0 mg

074-1806 Anti-Mouse IgG (H+L), HSA 1.0 mg

074-1506 Anti-Rabbit IgG (H+L) 1.0 mg

Biotin-Labeled

16-10-06 Anti-Human IgG (H+L) 0.5 mg

16-18-06 Anti-Mouse IgG (H+L), HSA 0.5 mg

16-15-06 Anti-Rabbit IgG (H+L) 0.5 mg

Labeled Streptavidin

15-30-00 AP-labeled 0.5 mg

14-30-00 HRP-labeled 0.5 mg

Assay Support Reagents

50-84-00 Coating Solution Concentrate 50 mL

50-63-00 Wash Solution Concentrate 800 mL

Blocking Solutions

50-61-00 10% BSA Diluent/Blocking Solution 200 mL

50-61-10 10% BSA Diluent/Blocking Solution 1 L

50-82-01 Milk Diluent/Blocking Solution 200 mL

Conjugate Stabilizers

55-15-00 APStabilizer 200 mL

54-15-01 HRPStabilizer 200 mL

Stop Solutions

50-85-01 ABTS Stop Solution 200 mL

50-89-00 APstop Solution 200 mL

50-85-05 TMB Stop Solution 400 mL

50-85-06 TMB Stop Solution 1 L

50-85-30 TMB BlueSTOP Solution 400 mL

50-85-41 TMB BlueSTOP Solution 1 L

To order, call 800.638.3167 or 301.948.7755, fax 301.948.0169,online at www.kpl.com or contact your local KPL Sales Partner.ISO 9001:2000 RegisteredML299-03

HSA: Human serum adsorbed

SEE MORE with KPL!

ELISA Brochure

Documents

Transcript of ELISA Brochure