EFFECTS OF AN ELECTROMAGNETIC FIELD ON BOAR SPERM PERMEABILITY Alita Loveless, PGY 4 M. Sellers, L....
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Transcript of EFFECTS OF AN ELECTROMAGNETIC FIELD ON BOAR SPERM PERMEABILITY Alita Loveless, PGY 4 M. Sellers, L....
![Page 1: EFFECTS OF AN ELECTROMAGNETIC FIELD ON BOAR SPERM PERMEABILITY Alita Loveless, PGY 4 M. Sellers, L. Welch, S. Prien.](https://reader036.fdocuments.us/reader036/viewer/2022062423/5697c0281a28abf838cd6934/html5/thumbnails/1.jpg)
EFFECTS OF AN ELECTROMAGNETIC FIELD
ON BOAR SPERM PERMEABILITY
Alita Loveless, PGY 4M. Sellers, L. Welch, S. Prien
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Introduction Electromagnetic fields are everywhere
Produced by alternating current
Can affect and possibly damage human tissues by thermal effect and disruption of molecular structure
Questionable links to various medical problems
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Previous Experiments Exposure to extremely high EMFs caused a
decrease in semen parameters
Parameters are indicative of cell function
Short-term Effects of EM Fields on Sperm Motility in Culture
010203040506070
0 h 0.5 h 1 h 1.5 h 2 h 2.5 h 3 h
time
Mot
ility
(%)
Control
EMF
P < .001
Short-term Effects of EM Fields on Sperm Forward Progress in Culture
00.5
11.5
22.5
33.5
4
0 h 0.5 h 1 h 1.5 h 2 h 2.5 h 3 h
time
Forw
ard
Pro
gres
sion
(1
-5 s
cale
)Control
EMF
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Questions A way to detect signs of cell damage
before cell function was altered
Mechanism leading to decreased cell function
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Theoretically… Long-term depolarization of a cell membrane
when subjected to an EMF
Shift in membrane permeability
Potential effect on osmoregulation and disruption of cell function
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Common Lab Equipment Hood 8 mG Centrifuge 36 mG Incubator
Upper shelf 86 mG Middle shelf 32 mG Lower shelf 2 mG
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Materials and Methods Porcine semen samples Incubated at 37 degrees Celcius with and
without exposure to an EMF Removed samples, combined them with
fluoroscein Examined the samples under a fluorescent
microscope to determine percentage permeabilized
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Results Decreases in standard
semen parameters
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Results Effects on path
straightness and linearity
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Results Increased cell permeability with EMF exposure
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Conclusion Short-term exposure
to an EMF appears to increase sperm permeability and alter cell physiology
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Clinical Relevance Assisted Reproductive
Technology labs Environment is critical Could basic equipment
be affecting our success rates?
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Resources and Thanks… Thanks to Lisa Welch, Matt Sellers, and Dr.
Prien Previous research: Dr. Naghma Farooqi WHO EMF Project
http://www.who.int/peh-emf/en/