Effect of The Different Auxins and Cytokinins in Callus Induction, Shoot,and Root Regeneration in...

8/13/2019 Effect of The Different Auxins and Cytokinins in Callus Induction, Shoot, and Root Regeneration in Sugarcane

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EFFECT OF THE DIFFERENT AUXINS

AND CYTOKININS IN CALLUS

INDUCTION, SHOOT,AND ROOT REGENERATION IN

SUGARCANE

Presented by:Arghya Narendra Dianastya

5620000087

Departement of Agronomy

Kasetsart University, Kamphaeng Saen


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INTRODUCTION

Modern sugarcane varieties are breeding of

species, involving crosses between the

species Saccharum officinarumL. and

S. spontaneumL (Heinze,1987).

Sugarcane is a tall growing monocotyledonous

crop.

Sugarcane has ability to store high

concentrations of sucrose, or sugar, in the

internodes of the stem.


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SUGARCANE PRODUCER

In 2010, Brazil was the largest producer at 719 million tonnes

(FAO, 2013)


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GLOBAL PRODUCTION AND

CONSUMPTION

FAOscurrent estimate for world sugar production in 2012/13, at 180million tonnes, (new record production)


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SUGARCANE AREA

HARVESTED


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INTRODUCTION

What can we do to improve the quality and

productivity of sugarcane


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IMPROVEMENT OF SUGARCANE PRODUCTIVITY AND

QUALITY

Plant improvement

Conventional methode

Plant biotechnology (ex: plant tissue

culture)

Cultural practice improvement


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ADVANTAGES OF TISSUE

CULTURE

Plant regeneration through tissue

culture technique would be a betteralternative for improving the quality,

production, and improvement of crop

through the rapid production of clones(Jabbot & Atkin, 1987)


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WHAT IS TISSUE CULTURE ?

Plant tissue culture can be defined as aprocedure used to mantain and grow plant tissue(callus, cells, protoplasm) and organ (stem, root,

embryos) in aseptic or in vitroculture.

Basic Concept is Totipotency:Totipotency ability in individual plant cells that can

be regenerated to a whole plant by modulatingculture conditions(Reinert, 1959).


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(Hartmann et.al.,1990 )

PATHWAY OF PLANT TISSUE AND CELL

CULTURE


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OBJECTIVES OF THE

RESEARCH

To analyze the effect of different concentration

of auxins and cytokinins in Callus Induction,

Shoot,

and Root Regeneration in Sugarcane.

To analyze the effect of different level of pH and

sucrose in root regenenation of sugarcane.


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MATERIAL

Sugarcane Cultivar CO 671(3-5 months old)

MS Medium - Murashige and skoog medium

Cytokinin

BAP

Kn

Auxin IBA

IAA

NAA


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METHODOLOGY


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RESULT


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CALLUS INDUCTION

Effect of different concentration, auxin and 2,4-D on callus inductionfrom leaf sheath explants of sugarcane


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CALLUS INDUCTION

The best cal lus induct ion sup lement

3.0mg/L of 2,4-D = 85% explant produced callus

No callus induction IBA


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SHOOT REGENERATION

Effect of the cytokinin (BAP,Kn) and the auxin (IBA,NAA) at different

concentrations and combination is Ms medium on shoot on shootregeneration from the callus tissue of sugarcane


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SHOOT REGENERATION

cont..

Effect of the cytokinin (BAP,Kn) and the auxin (IBA,NAA) at different

concentrations and combination is Ms medium on shoot on shootregeneration from the callus tissue of sugarcane


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SHOOT REGENERATION

BAP+IBA

The best respond 1.0+0.5 mg/L = 95

% produced shoot

BAP+NAA

The best respond 1.0+0.5 mg/L =

94% produced shoot


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ROOT REGENERATION

Effect of different auxin on formation of root on the invitro grownmicro shoots Cultured


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ROOT REGENERATION

The Best Root ing Media

strength of MS medium + 3mg/L NAA = 95%

explant produced root

Comparison Media Used

NAA and IBA were better response than IAAfor

producing roots.


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CONCENTRATION OF

SUCROSE

Effect of sucrose concentration on rooting of invitro differentiatedshoots of sugarcane


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CONCENTRATION OF

SUCROSE

The Best Sucrose Concentrat ion

50 gram/L of sucrose = 96% explant

produced root


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pH LEVEL

Effect of different pH level on rooting of in v i t rodifferentiated Shoots ofsugarcane


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pH LEVEL

The Best pH Level

pH 5.7 =96% explant produced root

The Poorest pH Level

pH 3 = 0 % explant produced root


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CONCLUSION

The best callus induction was observed at 3.0 mg/L of 2,4-D

The best shoot regeneration was observed

at 1.0 mg/L and 0.5 mg/L of (BAP + NAA)

The best root regeneraration was observed at 3.0 mg/L of NAA

The best sucrose concentration 50 gram/L of Sucrose

The best pH level

pH 5.7


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REFERENCE

Gopitha A., Lakshmi B., Senthilmanickam J. 2010. Effect

of the different auxins and cytokinins in callus induction,

shoot, root regeneration in sugarcane. International

Journal of Pharma and Bio Science. Vol.1/Issue-3/Jul-

Sep.2010.


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Reinert J (1959) The control of morphogenesisand induction of adventitious embryos in cellcultures of carrots. Planta53:318333.

Jabbott A.J and Atkin R.A. 1987 ImprovingVegetatively Propagated Crop. Academic PressLimited.

Hartmann H.T., Kester D.E., Davies F.T., andGeneve R.L. 1997. Plant Propagation: Principlesand Practices, Sixth Edition. Prentice HallInternational, Inc.


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ACKNOWLEDGMENTS

Kasetsart University AdvisorProf. Sontichai Chanprame, Ph.DProf. Sermsiri Chanprame, Ph.D

Jember University AdvisorDr. Kacung Haryono

CAB (Center of Agriculture Biotechnology)

And for All that have helped me to study in Kasetsart University


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THANK YOUFOR

YOUR ATTENTION

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