ECPGR Pescia

22 March 2010 – Pescia, Italy

ECPGR updateECPGR updateL. MaggioniL. Maggioni


ECPGR - ECPGR - www.ecpgr.cgiar.org

A cooperative programme for conservation and use of PGR in Europe for breeding and research

43 member countries- owners- contributing funds and

implementing activities- coordination within country


ECPGRECPGR objectivesobjectives

• Facilitate in situ and ex situ conservation, and increased use of PGR in Europe

• Strengthen collaboration in Europe• Increase planning of joint activities, including

development of joint project proposals• Encourage sharing of responsibilities for PGR

conservation in Europe• Promote public awareness on PGR conservation

and use


Phase VIII (2009-Phase VIII (2009-2013)2013)

5-year budget: 2.76 Mo. Euro – Network operations – Coordination– AEGIS coordination and activities– Contribution to EURISCO

4 priority areas– Task sharing and capacity building (top priority)– Characterization and evaluation – In situ and On-farm conservation and management– Documentation and Information


ECPGR Structure ECPGR Structure


Italian partners collaborating with Italian partners collaborating with ECPGRECPGR

National Coordinator: Carlo FideghelliCRA - Centro di Ricerca per la Frutticoltura, Roma

Working Group members: Avena and Barley: V. Terzi and ?? - CRA - Fiorenzuola d'Arda (PC)Wheat: A. Brandolini - CRA - S. Angelo Lodigiano (MI)Forages and In situ Network: V. Negri - Università degli Studi di PerugiaMalus/Pyrus and Prunus: M. Bergamaschi and D. Giovannini - CRA – ForlìVitis: M. Gardiman - CRA – Conegliano (TV)Grain Legumes and Potato: A. Carboni and B. Parisi - CRA – BolognaBeta and Fibre Crops (Flax and Hemp): G. Mandolino CRA- RovigoMedicinal and Aromatic Plants: C. Vender - CRA- Villazzano (TN)Allium: V. Miccolis - Università degli studi della Basilicata, PotenzaBrassica: F. Branca - Universita’ di CataniaCucurbits and Umbellifer Crops: N. Ficcadenti and N. Acciarri - CRA-

Ascoli PicenoLeafy Vegetables: A. Falavigna - CRA – Montanaso Lombardo (MI)Solanaceae: G. Polignano - Istituto di Genetica Vegetale, CNR, Bari


Italian partners Italian partners - Specific roles, activities - Specific roles, activities

Ex situ National Inventory Focal Point: P. Engel - CRA-Roma: provider of national data to Eurisco: 21,000 accessions

On-farm National Inventory Focal Point: V. Negri, Universita’ di Perugia

In situ National Inventory Focal Point: VacantChair of Brassica WG: F. Branca, Universita’ of CataniaChair of On-farm conservation WG: V. Negri, Universita’ di PerugiaMember of Inter-regional Cooperation NCG: P. Engel, CRA-Roma Managers of ECPGR Central Crop Databases:

CRA- Bologna: Hemp Universita’ di Firenze: Minor Fruit TreesIstituto di genetica vegetale, Bari: Vicia spp.

Universita’ di Perugia: On farm / in garden contacts


Cereals – Avena WG Cereals – Avena WG

Planned activities: • Avena WG meeting – October 2010, Romania(Implementing AEGIS, selection of MAA,

procedures for Avena wild relatives multiplication)

• Survey of wild oat populations:• Cyprus and Greece(2009)• Italy and Spain (2010)


Cereals – Barley WGCereals – Barley WG

Planned activities: • Barley WG meeting – 2011, Cyprus

(Preparation for AEGIS)

• Definition of MAAs, with focus on accessions originating from the own country of the holding genebanks;

• Database updated, C&E data searchable, coordination with other systems using web service technology;

• Inventory of barley genetic stocks collections in Europe


Cereals - Wheat WG Cereals - Wheat WG

Planned activities: Wheat WG – May 2011, Slovakia

(Preparation for AEGIS)

• Definition of MAA accessions;• Agreement on crop quality standards;• Improvement of the wheat database by introduction of

20 descriptors;• Overview of genebank work relating to traits

associated with climatic change


Cereals - other Cereals - other

Planned activities:• Pre-breeding workshop – Nov. 2009, Sweden

(Establish a pre-breeding cooperation between breeders, genebanks and researchers)

• Re-establish contacts with contributors to Maize DB


Forages WG Forages WG

Planned activities:• Forages WG meeting: 28-29 April 2010, Germany

• Documentation and information: Forage CCDBs – Carry out identification of primary holder for remaining

CCDBs + safety storage– Poa, Lolium, Trifolium, Festuca, Dactylis, etc.:Reduction of

unnecessary seed multiplication through task sharing (responsibility for maintenance of special acc. (MOS and PRIMCOLL determined)

– Enter characterization and evaluation data to CCDBs– Create „forage crop portals“ to raise public awareness and

use of forages genetic resources (with EPGRIS3) – Lolium


Forages WG Forages WG

• In situ & on-farm conservation and management

– Recommendations for set of forage CWRs for in situ conservation sites

• Characterisation and evaluation

– Support genotyping initiatives with the aim of identifying similar accessions and understanding diversity of European forage crops

– Increased knowledge amongst the WG members on the use of molecular markers for forages in identifying similar accessions


Fruit – Prunus WG Fruit – Prunus WG

Planned activities:• Prunus WG meeting – Forli, 7-9 Sept 2010• Prunus ad hoc meeting on AEGIS, to be defined• Ad hoc meeting of Fruit DB managers, 2011

• Implementation of AEGIS (Complete databases; identify MAAs, Implement Quality System, Safety duplication, Health status monitoring)

• Prunus DB development and maintenance


Fruit – Malus/Pyrus WG Fruit – Malus/Pyrus WG

Planned activities:• Malus/Pyrus WG meeting – jointly with ad hoc meeting on fruit

synonyms and descriptors - 2011

• Harmonization of the Pyrus and the Malus ECCDBs in accordance with the Cherry DB structure & Updating of the Malus & Pyrus Databases

• Standardization of the use of Molecular markers as results of the workshop (with Prunus and Vitis people) at EMR East-Malling, 7-8 December 2006. Publication of the validated results and start a first set of accessions from different collections.

• Update and improvement of characterization and evaluation descriptors for Malus and Pyrus and their inclusion in the DBs

• Finalizing standard guidelines for inclusion of photographs in the Malus and Pyrus databases

• Implementation of case studies of characterization and evaluation descriptors on apple and pear


Fruit – Malus/Pyrus WG Fruit – Malus/Pyrus WG

Planned activities:• Validation of the Pyrus data of ‘Synonyms’ • Defining and publishing common guidelines for the in

situ and on-farm conservation of Malus/Pyrus (in collaboration with the Prunus and Vitis WG’s)

• Safety duplicate the unique material by sharing responsibilities

• Stimulate the actions of the different TF : (1) “European Pomology Bibliography TF”; (2) “Traditional Knowledge TF”; (3) “Descriptors TF”; (4) “Malus and Pyrus species TF”


Fruit – Vitis WG Fruit – Vitis WG

Planned activities:

• Vitis WG meeting, Sept. 2012, Siebeldingen, Germany

• Vitis DB development and maintenance


Oil and Protein Crops Oil and Protein Crops NetworkNetwork

Planned activities:•Grain legumes WG meeting, 22-23 April 2010, Antalya, Turkey

– Review progress of Phase VIII projects and workplan

– Discuss development and engagement with AEGIS project

– Prepare proposals for new workplan

•Other outputs:– Grain Legume Climate Change Monitoring Network: collecting

data on insect pests and flowering time data– Uploading of revised grain legume CCDB to web

– Development of in situ workplan for Grain legumes


Sugar, Starch and Fibre Sugar, Starch and Fibre Crops NetworkCrops Network

Planned activities:• Network Coordinating Group and ECCDB Manager Meeting, Sept. 2009, Quedlinburg, Germany

• Network Coordinating Group meeting March 2013

• Participation in the Meeting of the In Situ Network, Madeira, Portugal, September 2010


Sugar, Starch and Fibre Sugar, Starch and Fibre Crops Network - Potato WGCrops Network - Potato WG

Planned activities:

• Potato WG meeting, 2011– Update of central DB– Definition of MAA accessions– Agreement on task sharing– Discuss molecular & phenotypic

characterization issues


Sugar, Starch and Fibre Sugar, Starch and Fibre Crops Network - Beta WGCrops Network - Beta WG

Planned activities:

•Beta WG meeting, Cappelle-en-Pévèle, France, 2011

– Discussion on in situ management data recording using CyberTracker.

– Development of an implementation plan for Beta genetic reserves– Drafting of project proposals (collecting, genetic diversity, evaluation, data

management, in situ management). A focus may be set on Beta nana (possible indicator for climate change) and other Beta species with limited distribution area.

– Monitoring of a genetic reserve of Beta patula. Discuss further collaboration on Beta and future collaboration on genera of mutual interest (Avena, Brassica, Vicia and others)

– Sampling of Beta nana for geneflow analysis five years after last visit of the Olympos site. This action is actively supported by the USDA/ARS


Sugar, Starch and Fibre Sugar, Starch and Fibre Crops Network - Medicinal Crops Network - Medicinal And Aromatic Plants WGAnd Aromatic Plants WG

Planned activities:• MAP WG meeting, Turkey, October 2009

• The Origanum project: “Conservation and characterization of oregano (Origanum vulgare L.) wild populations in Europe”


Sugar, Starch and Fibre Sugar, Starch and Fibre Crops Network - Fibre crops Crops Network - Fibre crops (flax and hemp) WG(flax and hemp) WG

Planned activities:

• Fibre Crops WG meeting, Sumperk, Czech Republic, 7-9 July 2010


Vegetables Network -Vegetables Network -

Planned activities:

• Vegetables Network meeting, Catania, Italy, 10-12 November


Issues of Network-wide Issues of Network-wide interestinterest (VEGNET meeting, November (VEGNET meeting, November 2009)2009) • Updating information on stakeholder’s practices – in

support of the AEGIS Quality System • Role of National Coordinators – recommendation to WG

members to proactively interact with them• EURISCO and CCDBs – Recommendation that WG

member encourage NC to provide data to EURISCO• Informing scientific community of WG achievements –

WGs encouraged to submit papers to scientific journals on behalf of WG

• Merging of Vegetable WGs – Negative reply of VEGNET• Establishment of Network on Cryopreservation –

suggestion


Allium WGAllium WG

Planned activities:• Meeting with EURALLIVEG (3 members), 2010 • Allium WG meeting, Greece, 2011• Garlic project - fingerprinting of 158 accessions

– discussion of results:MAAs identified for garlic


Brassica WG – Phase VIIIBrassica WG – Phase VIII

Planned activities:• Brassica WG meeting• Morphological and nutraceutical characterization of B.

rapa and wild Brassica species• Molecular characterization of the above (AFLP, SSR)


Cucurbits WGCucurbits WG

Planned activities:• Cucurbits WG meeting, Georgia, October 2010

– Workplan reviewed and new tasks agreed, mainly related to AEGIS implementation

• Implementation of safety duplication • Development and implementation of specific

algorithms to facilitate the identification of duplicates• Strengthen collaborations with the In situ and on farm

Network


Leafy vegetables WGLeafy vegetables WG

Planned activities:• Leafy vegetables WG meeting, Slovenia, October

2011• Project activities to develop a unified

molecular characterization protocol for lettuce


Solanaceae WGSolanaceae WG

Planned activities:• Solanaceae WG meeting, Izmir, Turkey, March 2011• Development and implementation of specific

algorithms to facilitate the identification of duplicates • Implementation of safety duplication


Umbellifer Crops WGUmbellifer Crops WG

Planned activities:• Umbellifer Crops WG meetings, 2011 and 2013• Regeneration of collections• Conservation of collections• Characterization and evaluation• Studies on minor crops• Database update• Wild Umbellifer relatives project• Implementation of AEGIS on carrot as a model crop


Documentation and Information Documentation and Information Network Network

Planned activities:• Inter-regional workshop, Uganda, March 2009

(Capacity building in documentation and information in other regions) • Training of National Inventory Focal Points, Prague, June 2009

(Training on establishing and further developing the ex situ National Inventories; Update NI and improve the quality and quantity of the data; Registration of material in the MLS of the ITPGR and sMTA reporting; Improve uploading NI to EURISCO)

• NCG meeting, Rome, February 2010(Review of progress in the D&I network)

• In situ NI Focal Points, Bonn, June 2010(Training on establishing and further developing the National Inventories - in situ and on farm)

• Inter-regional Workshop, 2010 or 2011(Capacity building in documentation and information in other regions)

• NCG meeting, 2013(Review of progress in the D&I network)


In situ and on-farm In situ and on-farm conservation Networkconservation Network

Planned activities:– First meeting of the two Working Groups ("Wild

species in genetic reserves" and "On-farm conservation" - jointly with AEGRO meeting 13-16 September 2010, Madeira, Portugal

– 2nd Meeting of Working Group on On-farm conservation and management (on European On-farm methodologies) – Sept. 2012


In situ and on-farm In situ and on-farm conservation Networkconservation Network

Documentation and information:

– Europe wide national landrace inventories – Publication of European On-farm experience and

methodologies meeting proceedings– Publication of Landrace Newsletters – Publication of Inter-sector Conservation and

Application meeting proceedings In situ & on-farm conservation and management

– Identification of European sites for on farm conservation within AEGRO project in cooperation with the Forages WG


Inter-regional cooperation Inter-regional cooperation NetworkNetwork

Planned activities:

• Inter-regional workshops on IT implementation Uganda, March 2009

• Meeting of Inter-regional Cooperation Network Coordinating Group


Other relevant ECPGR Other relevant ECPGR activities during Phase activities during Phase VIIIVIII

• ECPGR External Independent Review: July 2010

• All Network Coordinating Groups meeting in February 2011?

• Mid-term Steering Committee meeting in June or October 2011, Vienna

• End of Phase Steering Committee meeting in 2013


http://eurisco.ecpgr.orghttp://eurisco.ecpgr.org


• Provides information about ex situ plant collections

maintained in Europe

• Based on a European network of 44 ex situ National

Inventories (NIs)

• Current content: passport data on > 1 million samples

from 41 countries

• Service to International Treaty– Registration of accessions in the MLS and sMTA

reporting


EURISCO EURISCO (Roles and responsibilities)(Roles and responsibilities)

1. Responsible of data sources & merges2. Ensure data quality & accuracy 3. Decide on data availability 4. Commit to provide data in the EURISCO-

MCPD data format Nat

ion

alN

atio

nal

1. Automatic checking that NI data conform to the standard 2. Provide feedback to National Partners 3. Import NI data into EURISCO4. Develop and maintain Web siteC

entr

alC

entr

al


Inclusion of Material in the MLS by Inclusion of Material in the MLS by the Contracting Parties to the the Contracting Parties to the ITPGRFAITPGRFA(via EURISCO) (via EURISCO)

Austria 7.268 acc.Czech Republic 32.597 acc.Germany 108.562 acc.Ireland 1.117 acc.Netherlands 16.441 acc.Nordic countries 14.494 acc.Poland 14.758 acc.Portugal 813 acc.Spain 15.755 acc.

Total (November 2009) = 211.805 accessionsSource:http://eurisco.ecpgr.org


National Focal Point

regional

global

national

CGIAR / SINGER

NLD ISL NOR DNK SWE FIN USA

CGN GRINNGB

DEU

BLE

FRA

BRG

RUS

VIR

....

NFPs/NIs..

CAN

North America / GRIN

Institutes IPK JKI others

Existing information Existing information infrastructure (global)infrastructure (global)

Global-IS, Treaty Art 17 FAO/Bioversity International/Trust

GENESYS

Europe / EURISCO


C&E data from EuropeC&E data from Europe

A concept to include C&E data in EURISCO, developed by the ECPGR Documentation and Information Network

(see http://www.epgris3.eu/):

• Simple exchange format containing: Experiment; Trait; Genotype; Score in order to gather whatever non-standardized C&E data already exists

• Uploading mechanism (aligned with current EURISCO upload mechanism)

• Downloading mechanism (to be adapted to different user group needs)

• Implementation to be carried out in collaboration with Bioversity (GIGA project) or EU-funded project


(www.aegis.cgiar.org)(www.aegis.cgiar.org)

A European Genebank Integration System


AEGIS ObjectiveAEGIS Objective

To create A European Genebank Integrated System for plant genetic resources for food and agriculture, aimed at conserving the genetically unique and important accessions for Europe and making them available for breeding and research.


Perceived Benefits of Perceived Benefits of AEGISAEGIS

• Improved quality standards of conserved

material

• Improved security of germplasm through long-

term commitment and systematic safety-

duplication

• Facilitated access to and availability of

germplasm

• Increased effectiveness in regeneration

• Cost efficient conservation activities

• Reduced duplication of germplasm material

• Improved sharing of knowledge and information


Key components of AEGISKey components of AEGIS

1. A Strategic Framework for the Implementation of a European Genebank Integrated System - A Policy Guide

2. Formal agreement with countries (MoU)

and institutions within countries (Asso-

ciate Membership) – 17 members

3. European Collection (contains identified and agreed MAAs for each crop; in public domain; readily available) (to be developed)

4. Technical and operational quality standards (to be developed)


Key components of AEGISKey components of AEGIS

5. Quality management system (reporting; monitoring; capacity building) (to be developed)

6. EURISCO as information portal for European Collection (to be further developed)


AEGIS and the European AEGIS and the European CollectionCollection

• European Collection will consist of dispersed accessions (MAAs), i.e. a virtual European genebank

• Through signing the MoU countries accept responsibilities for long-term conservation and availability of European accessions, and to

• conserve/manage according to quality standards• Conservation/management strategies for each crop

are prepared by respective Crop WG/NCG and approved by SC


AQUAS – AEGIS quality AQUAS – AEGIS quality systemsystem

• Development of a quality management system, including generic and crop operational standards, reporting and monitoring

• Discussion paper endorsed by SC; on the AEGIS website

• Brassica, Forages and Grain legumes WGs requested to test the template for an operational genebank manual

• Draft of generic genebank standards by Secretariat


Competitive AEGIS GrantCompetitive AEGIS Grant

A total of € 103,000 has been made available for Phase VIII of the ECPGR programme

To support activities that will directly contribute to the establishment and/or operation of AEGIS

17 proposals were received by the deadline. Only 3 will be funded. Another call at the end of 2010– Assessment of umbellifers unique material– Pea germplasm management– Cryopreservation of garlic inflorescences


Thank you

ECPGR Pescia

Technology

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