Ecoletter Biolasting eng 02 · 2015-01-23 · ECOLETTER Periodic publication of trials that test...
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ECOLETTER Periodic publication of trials that test the efficacy and properties of the products of Futureco Bioscience Evaluation of the efficacy of Biolasting® as a biofungicide for the control of Monilia laxa on peach at post-harvest laboratory conditions. Almazán, M., Lara, J.M., and Fernández, C. Issue 13, 22 January 2015 Introduction Biolasting® is a product of natural origin formulated with Citric Extracts with tested fungicide action. In post-harvest applications, Biolasting® is efficient for the control of fungi, expanding the life of fruits and vegetables, that uses to be shortened during post-harvest storage by fungi infections. In the case of storage of fruits such as Peach, a common disease is the brown rot caused by Monilia laxa, one of the most important diseases of peach and other fruits of the genre Prunus. Infection by this fungus can occur in either in the field as also in the harvested fruits during its storage and commercialization, and can give significant economic losses. In the European Union applications of phytosanitary products for the control of this pathogen are only authorized in pre-harvest, with methods that have been demonstrated to be ineffective for the control of this pathogen. In this trial, Futureco Bioscience tests the efficacy of Biolasting® in the control of Monilia laxa in peach, in post-harvest laboratory conditions at 4º C and at 18ºC. Materials and Methods Efficacy of Biolasting® for the control of Monilia laxa was evaluated in the variety of peach “Yellow Calanda” in laboratory conditions at four doses (1, 2, 3 y 4% v/V). The treatment was performed by immersion of the fruits in every concentrate of Biolasting® during 5 minutes, 1 hour later from the artificial inoculation of the peaches with a suspension of M. laxa at a concentration of 10 4 conidia/mL. Control samples were added to the trial, as a negative control (not inoculated nor treated) and a positive control (inoculated but not treated). After 26 days of incubation (26 days post-inoculation, 26 DPI) at 4ºC, the damaged area due to the pathogen on the peaches was calculated (expressed as severity from 0 to 1) and the quality of the fruits through the measurement of Total Soluble Solids (TSS) on 5 fruits per treatment. The rest of fruits were maintained 5 days more at 18ºC, when the damaged area was again calculated, as also the efficacy of each treatment. Percentage of efficacy regarding the percentage of the damaged area was calculated with the Abbot formula: %Efficacy=[(Non treated-treated)/Non treated]·100 Toxicity was evaluated according to the guide EPPO PP 1/135 (3) and expressed through the ERWC scale (Non toxic=1, Acceptable limit=4, Inacceptable=6). Statistical test LSD (Least significant difference) was applied in order to discard treatment differences that could occur with the test ANOVA (method Newman-Keuls) previously performed with a 95% confidence. Results BIOLASTING® is a product from natural origin that acts as an organic Fungicide-Bactericide. After harvest, BIOLASTING® has an antimicrobial response against a wide range of fruit pathogens, including fungi and bacteria. Additionally, BIOLASTING® is rich in organic acids (ascorbic acid and bioflavonoids); both compounds have antioxidant properties, allowing a synergistic effect that helps to preserve the natural aroma of fruits and vegetables in storage Table 1. Damaged surface par treatment (4 and 18ºC) and Total Soluble Solids. Parameter % Damage area Efficacy % Damage area Efficacy TSS Measurement unit 0-100% 0-100% 0-100% 0-100% ºBRIX Fruits 12 12 7 7 7 Evaluation interval 26 DPI (4ºC) 26 DPI (4ºC) 31 DPI (18ºC) 31 DPI (18ºC) 26 DPI # Name Dose 1 6 1 Control (+) 0,000 a 100,00 0,000 100,00 13,13a 2 Control (-) 87,868b 0,00 98,897 0,00 11,74b 3 Biolasting® 1%v/V 72,794b 17,15 89,916 9,081 12,52ab 4 Biolasting® 2%v/V 59,559bc 32,22 80,672 18,428 12,55ab 5 Biolasting® 3%v/V 24,559 ac 72,05 31,765 67,881 12,82a 6 Biolasting® 4%v/V 18,984 a 78,39 31,988 67,655 12,99a LSD 37,702 - 56,421 - 0,876 Standard Deviation 42,741 - 44,614 - 0,625 CV 94,381 - 75,644 - 0,049 Media 45,010 - 59,640 - 12,623 Graphic 1. Percentage of efficacy on the damaged surface at 4ºC and 18ºC
Transcript of Ecoletter Biolasting eng 02 · 2015-01-23 · ECOLETTER Periodic publication of trials that test...
ECOLETTER Periodic publication of trials
that test the efficacy and properties of the products of
Futureco Bioscience
Evaluation of the efficacy of Biolasting® as a biofungicide for the control of Monilia laxa on peach at post-harvest laboratory conditions.
Almazán, M., Lara, J.M., and Fernández, C.
Issue 13, 22 January 2015
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Introduction Biolasting® is a product of natural origin formulated with Citric Extracts with tested fungicide action. In post-harvest applications, Biolasting® is efficient for the control of fungi, expanding the life of fruits and vegetables, that uses to be shortened during post-harvest storage by fungi infections.
In the case of storage of fruits such as Peach, a common disease is the brown rot caused by Monilia laxa, one of the most important diseases of peach and other fruits of the genre Prunus. Infection by this fungus can occur in either in the field as also in the harvested fruits during its storage and commercialization, and can give significant economic losses. In the European Union applications of phytosanitary products for the control of this pathogen are only authorized in pre-harvest, with methods that have been demonstrated to be ineffective for the control of this pathogen. In this trial, Futureco Bioscience tests the efficacy of Biolasting® in the control of Monilia laxa in peach, in post-harvest laboratory conditions at 4º C and at 18ºC.
Materials and Methods Efficacy of Biolasting® for the control of Monilia laxa was evaluated in the variety of peach “Yellow Calanda” in laboratory conditions at four doses (1, 2, 3 y 4% v/V). The treatment was performed by immersion of the fruits in every concentrate of Biolasting® during 5 minutes, 1 hour later from the artificial inoculation of the peaches with a suspension of M. laxa at a concentration of 104 conidia/mL. Control samples were added to the trial, as a negative control (not inoculated nor treated) and a positive control (inoculated but not treated). After 26 days of incubation (26 days post-inoculation, 26 DPI) at 4ºC, the damaged area due to the pathogen on the peaches was calculated (expressed as severity from 0 to 1) and the quality of the fruits through the measurement of Total Soluble Solids (TSS) on 5 fruits per treatment. The rest of fruits were maintained 5 days more at 18ºC, when the damaged area was again calculated, as also the efficacy of each treatment. Percentage of efficacy regarding the percentage of the damaged area was calculated with the Abbot formula: %Efficacy=[(Non treated-treated)/Non treated]·100 Toxicity was evaluated according to the guide EPPO PP 1/135 (3) and expressed through the ERWC scale (Non toxic=1, Acceptable limit=4, Inacceptable=6). Statistical test LSD (Least significant difference) was applied in order to discard treatment differences that could occur with the test ANOVA (method Newman-Keuls) previously performed with a 95% confidence.
Results
BIOLASTING® is a product from natural origin that acts as an organic Fungicide-Bactericide. After harvest, BIOLASTING® has an antimicrobial response against a wide range of fruit pathogens, including fungi and bacteria. Additionally, BIOLASTING® is rich in organic acids (ascorbic acid and bioflavonoids); both compounds have antioxidant properties, allowing a synergistic effect that helps to preserve the natural aroma of fruits and vegetables in storage
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Table 1. Damaged surface par treatment (4 and 18ºC) and Total Soluble Solids.
Parameter %
Damage area
Efficacy % Damage area
Efficacy TSS
Measurement unit 0-100% 0-100% 0-100% 0-100% ºBRIX
Fruits 12 12 7 7 7
Evaluation interval 26 DPI (4ºC)
26 DPI (4ºC)
31 DPI (18ºC)
31 DPI (18ºC) 26 DPI
# Name Dose 1 6
1 Control (+) 0,000 a 100,00 0,000 100,00 13,13a
2 Control (-) 87,868b 0,00 98,897 0,00 11,74b
3 Biolasting® 1%v/V 72,794b 17,15 89,916 9,081 12,52ab
4 Biolasting® 2%v/V 59,559bc 32,22 80,672 18,428 12,55ab
5 Biolasting® 3%v/V 24,559 ac 72,05 31,765 67,881 12,82a
6 Biolasting® 4%v/V 18,984 a 78,39 31,988 67,655 12,99a
LSD 37,702 - 56,421 - 0,876
Standard Deviation 42,741 - 44,614 - 0,625
CV 94,381 - 75,644 - 0,049
Media 45,010 - 59,640 - 12,623
Graphic 1. Percentage of efficacy on the damaged surface at 4ºC and 18ºC
For more information about this article please contact
[email protected] For more information about Biolasting®:
Futureco Bioscience SA, Avenida del Cadí 19-23
Sant Pere Molanta 08799 Olèrdola (Barcelona)
Spain
ECOLETTER #13 22.01.2015. Evaluation of the efficacy of Biolasting® as a bio-fungicide for the control of Monilia laxa on peach at post-harvest laboratory conditions.
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Images of the trials at 26 DPI at 4ºC and 18º C
Control -
Control +
Biolasting 1%
Biolasting 2%
Biolasting 3%
Biolasting 4%
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Graphic 2. Total Soluble Solids (TSS) evaluated in the juice obtained by each fruit.
Discussion and Conclusions
The results show that Biolasting® is significantly efficient in the control of the brown rot of the peach caused by Monilia laxa on peaches of the variety “Amarillo Calanda”, at the highest doses tested (3 and 4% v/v), either at 4ºC than at 18ºC although the percentage of efficacy observed at 18ºC is a 10% lower than at 4ºC.
The parameter of quality evaluated through the measurement of the Total Soluble Solids (TSS), remains at a level of negative control (not inoculated nor treated) in the treatment with Biolasting® at 3 and 4%, being significantly higher than those of the positive control. In summary, we can conclude that the best doses for the control of brown rot caused by Monilia laxa in peach is a 3% in volume to prevent putrefaction development and enlarge the life of the peach to optimize its sales and prevent economic loss.
More results of trials ran on field and more extensive post-harvest conditions are expected in order to demonstrate definitely the competitive advantage of this product of residue 0 in the market.
